Before Starting; Preparing Reagents - Omni 26-022 User Manual

Plant DNA Purification Kit

BEFORE STARTING

This method is suitable for the isolation of DNA from up to 100 mg of fresh/
frozen plant tissue and up to 30 mg of dried plant tissue.
Yields vary depending on source.
Materials and equipment to be supplied by user:
• Tabletop microcentrifuge capable of 10,000 x g
• Nuclease-free 1.5 mL microcentrifuge tubes
• Waterbath or incubator capable of 65ºC
• Bead Mill Homogenizer or vortexer
• β-mercaptoethanol (βME)
• Chloroform and isoamyl alcohol (24:1)
• Optional: RNase stock solution (20 mg/mL)
• 100% Ethanol

PREPARING REAGENTS

1. Dilute DW Buffer with 100% ethanol as follows and store at room temperature.
Kit
26-022
26-023 and 26-023B
2. Prepare or obtain a mixture of chloroform: isoamyl alcohol (24:1)
3. β-mercaptoethanol (βME) must be added to CTB Buffer before use
(20 µL βME/1 mL CTB).
4
100% Ethanol to be added
6 m L
80 mL