Introduction; Overview - Omni 26-022 User Manual

Plant DNA Purification Kit

INTRODUCTION

The Omni Plant DNA Purification Kit is designed for efficient recovery of
genomic DNA up to 40 kb in size from fresh, frozen, or dried plant tissue
samples rich in polysaccharides or having a lower DNA content. Up to 100
mg wet tissue (or 30 mg dry tissue) can be processed in less than 1 hour.
The system combines the reversible nucleic acid-binding properties of the
Omni Mini DNA Column matrix with the speed and versatility of spin column
technology to eliminate polysaccharides, phenolic compounds, and enzyme
inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction
digestion, and hybridization applications.

OVERVIEW

If using the Omni Plant DNA Purification Kit for the first time, please read this
booklet to become familiar with the procedures. This procedure relies on the
well-established properties of the cationic detergent, cetyltrimethyl ammonium
bromide (CTAB), in conjunction with the selective DNA binding of the Omni Mini
DNA Column matrix, to isolate high-quality DNA. Samples are homogenized
and lysed in a high salt buffer containing CTAB and extracted with chloroform
to remove polysaccharides and other components that interfere with many
routine DNA isolations and downstream applications. Binding conditions are
adjusted, and DNA is purified using an Omni DNA Mini Column. Salts, proteins,
and other contaminants are removed to yield high-quality genomic DNA
suitable for downstream applications such as endonuclease digestion, thermal
cycle amplification, and hybridization applications.
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