Omni 26-022 User Manual page 8

Plant DNA Purification Kit
9. Insert an Omni DNA Mini Column into a 2 mL Collection Tube.
10. Transfer the entire sample (including any precipitate that may have
formed) to the Omni DNA Mini Column.
11. Centrifuge at 10,000 x g for 1 minute.
12. Discard the filtrate and the Collection Tube.
13. Transfer the Omni DNA Mini Column to a new 2 mL Collection Tube.
14. Add 700 μL DW Buffer.
NOTE: DW Buffer must be diluted with 100% ethanol prior to use.
15. Centrifuge at 10,000 x g for 1 minute.
16. Discard the filtrate and reuse the Collection Tube.
17. Repeat Steps 14-16 for a second DW Buffer wash step.
18. Centrifuge the empty Omni DNA Mini Column at maximum speed
for 2 minutes to dry the membrane.
NOTE: It is critical to remove any trace of ethanol that may otherwise
interfere with downstream applications.
19. Transfer the Omni DNA Mini Column to a nuclease-free 1.5 or 2 mL
microcentrifuge tube (not provided).
20. Add 50 μL EB Buffer (or sterile deionized water) heated to 65°C.
21. Centrifuge at 10,000 x g for 1 minute.
22. Repeat Steps 20-21 for a second elution step.
23. Store DNA at -20°C.
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