Related Manuals for Zeiss Focal CC
Summary of Contents for Zeiss Focal CC
- Page 1 Instruction Manual ZEISS Focal CC Charge Compensation for Serial Block Face Imaging...
- Page 2 Effective from: 03/2020 © 2021 Without the prior written consent of ZEISS, this document or any part of it must neither be translated nor reproduced or transmitted in any form or by any means - including electronic or mechanic methods, by photocopying, recording or by any in- formation or filing system.
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Page 3: Table Of Contents
Checking the Position of the Carbon Nozzle.......... 24 6.3.4 Adjusting the Height of the Carbon Nozzle ........... 25 6.3.5 Adjusting the Lateral Position of the Carbon Nozzle ......26 7 Troubleshooting ................27 Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000... - Page 4 Disposal ......................29 Decontamination ....................29 9 Technical Data and Conformity............30 Requirements to Run the Focal CC ..............30 Applicable Standards and Regulations..............30 Glossary ..................31 Index....................32 Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000...
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Page 5: General Information
1 General Information | 1.1 Text Conventions and Link Types 1 General Information This Instruction Manual is considered to be part of the Focal CC. Herein after referred to as De- vice. This Instruction Manual contains basic steps and safety information that must be observed during operation and maintenance. -
Page 6: Further Applicable Documents
Local and national health and safety regulations should be adhered to for the site and the use of health and safety the Device. Please consult with your ZEISS Sales & Service Partner in the event of these regulations regulations conflicting with the installation requirements of the Device. -
Page 7: Contact
ZEISS 1 General Information | 1.4 Contact 1.4 Contact If you have any questions or problems, please contact your local ZEISS Sales & Service Partner or one of the following addresses: Headquarter Phone: +49 1803 33 63 34 Fax: +49 3641 64 3439 Email: info.microscopy.de@zeiss.com... -
Page 8: Safety
(FESEM), equipped with an ultramicrotome stage. Focal CC is used to increase the image quality by eliminating charging effects. The effect of charg- ing of non-conducting specimens is eliminated by the injection of gaseous nitrogen above the specimen surface. -
Page 9: Safe Operating Condition
ZEISS service representative should be informed. The Device can only be operated after proper installation by a ZEISS service representative and in compliance with the operating conditions. Do not operate the Device until you have completely read and understood the entire documentation delivered with the Device. - Page 10 2 Safety | 2.3 Prevention of Hazards ZEISS To download the document: 1. Go to EIGA homepage www.eiga.eu. 2. Select Publications > EIGA Documents. 3. From the list, select Doc. 44/18. 4. Click Download. Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000...
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Page 11: Product And Functional Description Of Focal Cc
3 Product and Functional Description of Focal CC The Focal CC consists of a valved supply line and feedthrough on a defined chamber port and a precision attachment to the ultramicrotome inside the specimen chamber. The nozzle is fixed to the stage. - Page 12 3 Product and Functional Description of Focal CC | 3.2 Focal CC Ultramicrotome Attachment ZEISS Fig. 2: Focal CC attached to the ultramicrotome Fixing screws Silicone hose for nitrogen Mount Positioning spring Swing arm Pusher Knife holder Specimen Carbon nozzle Ultramicrotome Fig. 3: Focal CC, adjustment screws...
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Page 13: Internal Gaseous Nitrogen Interconnection
§ Imaging position As long as the Focal CC is activated in SmartSEM, the gas flow through the hose is always on, re- gardless which position the nozzle is in. The ultramicrotome software is not aware of the status of the Focal CC. - Page 14 3 Product and Functional Description of Focal CC | 3.3 Internal Gaseous Nitrogen Interconnection ZEISS Fig. 5: Chamber roof routing of Teflon hose and bayonet (silicone hose not shown) In all configurations, users must take care when closing the chamber door that neither the Teflon hose nor the silicone hose get squashed.
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Page 15: Principle Of Operation
Charge The Focal CC operates to avoid the charging effect. This is achieved by injecting a local flow of Compensation gaseous nitrogen onto the area of interest. -
Page 16: Installation
ZEISS 4 Installation Installation and commissioning are carried out by authorized ZEISS service representative. The in- stallation requirements are to be observed and adhered to. After installation or retrofitting, thor- oughly check that the Device is in a safe operational state, making sure in particular that all pro- tective covers (e.g. -
Page 17: First Operating Steps
Do not use the Focal CC gas flow at a default value of 100 %. This places a heavier strain on the vacuum system and will reduce the time period before expensive vacuum components need re- placement, and may speed up degradation in column reliability. -
Page 18: Exchanging The Specimen
HT to be turned on. If the microscope is fail- ing to reach system vacuum, check the status of the Focal CC gas flow since it is not automatically turned off when pumping the specimen chamber. -
Page 19: Selecting Focal Cc In The Software
Focal CC must be turned off when pumping the specimen chamber to vacuum. Info When Focal CC is selected in the software, quiet vacuum mode is not available. Fig. 7: The Charge Compensation section looks slightly different for GeminiSEM (left) and Sigma... -
Page 20: Controlling The Gas Injection
50 % CC pressure. This prevents arcing at the SE detector. The automatic switch off requires SmartSEM 6.06 or later. If you use SmartSEM 6.05 or earlier, you have to turn down the HT bias to the SE detector manually when the Focal CC is on. Prerequisite ü... -
Page 21: Stopping The Gas Injection
5.6.2 Stopping the Gas Injection Prerequisite ü The Focal CC is selected in the software, refer to Selecting Focal CC in the Software [ 19]. Procedure 1. For GeminiSEM: In the GeminiSEM Control panel, select the Control tab. -
Page 22: Care And Maintenance
Only conduct preventive measures described in the Instruction Manual. All tasks of maintenance, service and cleaning not described here must only be performed by an authorized ZEISS service representative. Any unauthorized intervention in the Device or any operation outside the scope of the intended use can lead to injuries and property damage, and voids all rights to warranty claims. -
Page 23: Maintenance Work
There is no warranty on the carbon nozzles. Info The Focal CC is supplied with a tool for star screws. Keep the tool in a safe place for future use. Fig. 8: Focal CC attached to the ultramicrotome... -
Page 24: Attaching The Silicone Hose
Refer to Attaching the Silicone Hose [ 24]. 5. Check and finely adjust the stop position, and clamp the locking screw. This should always be checked. Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000... -
Page 25: Adjusting The Height Of The Carbon Nozzle
2. Use a hex key to turn the height adjustment set screw clockwise to raise the carbon nozzle. INFO: If possible use a binocular viewer to make the height change clearly visible. 3. Tighten the fixing screws Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000... -
Page 26: Adjusting The Lateral Position Of The Carbon Nozzle
This takes advantage of the small amount of “play” in the whole structure when the fixing screws are slackened. Info The Focal CC is supplied with a tool for star screws. Keep the tool in a safe place for future use. Fig. 10: Focal CC, adjustment screws... -
Page 27: Troubleshooting
ZEISS 7 Troubleshooting 7 Troubleshooting The following table provides hints for solving common problems. If you cannot solve the problem or if you are unsure about a certain technical difficulty, contact your local ZEISS service represen- tative. Sympton Cause Measure No charge compensation Insufficient N₂... - Page 28 Alternatively, setup a multi ROI compensation required. acquisition instead and discard a small sacrificial ROI which is only used to restore equilib- rium after the beam is un- blanked. Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000...
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Page 29: Decommissioning And Disposal
They must be disposed of in accordance with applicable regulations (WEEE Directive 2012/19/EU). ZEISS has implemented a system for the return and recycling of devices in member states of the European Union that ensures suitable reuse according to the EU Directives mentioned. -
Page 30: Technical Data And Conformity
9 Technical Data and Conformity | 9.1 Requirements to Run the Focal CC ZEISS 9 Technical Data and Conformity This chapter contains important technical data as well as information on the conformity. 9.1 Requirements to Run the Focal CC §... -
Page 31: Glossary
Nitrogen Specially trained service expert, either ZEISS staff or authorized service partner Primary electron beam of ZEISS. Narrowly bundled beam of accelerated electrons that hit the specimen surface. Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000... -
Page 32: Index
19, 20, 21 Training 8 General Safety Information 8 Troubleshooting 27 Hazard Ultramicrotome 11 High pressure hazard 9 Mechanical hazard 9 Suffocation hazard 9 Hazards 9 Vacuum guidelines 17 Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000... - Page 33 ZEISS Index ZEISS Portal 7 Service agreements 22 Instruction Manual ZEISS Focal CC | en-US | Rev. 2 | 349561-8021-000...
- Page 34 Carl Zeiss Microscopy GmbH Carl-Zeiss-Promenade 10 phone: +49 1803 33 63 34 07745 Jena fax: +49 3641 64 3439 Germany info.microscopy.de@zeiss.com www.zeiss.com/microscopy...