Using Oil Immersion Objectives - KERN OBE-1 User Instructions

5.9 Using oil immersion objectives

The 100x objectives of the OBE-1 series are objectives which can be used with oil
immersion (they are always marked with the word "OIL"). Using these generates a
particularly high resolution for microscopic images.
To use oil immersion correctly, please follow these steps.
1. Put a drop of oil on the cover glass (with standard thickness of 0.17 mm) of the
object slide.
2. Lower the specimen stage and position the 100x objective in the beam path.
3. Bring the specimen stage or object slide to the objective very slowly until there is
slight contact.
4. Observe the object.
The object slide and objective must not be pressed against each other. The oil
constitutes the contact layer.
If the contact is made too jerky, there is a chance that existing air bubbles in the oil
cannot escape. This would have a negative impact on image clarity.
After use or before changing the slide, any components which have been in contact
with the oil must be cleaned thoroughly. See also 1.4 Maintenance and cleaning.
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