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Related Manuals for KERN OBL 146
Summary of Contents for KERN OBL 146
- Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-Mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Transmitted light phase contrast microscope KERN OBL-14, OBL-15 OBL 146, OBL 155, OBL 156 Version 1.2 01/2023 OBL-14-15-BA-e-2312...
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Page 2: Table Of Contents
KERN OBL-14, OBL-15 Version 1.2 01/2023 User instructions Phase contrast microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................6 Technical data / Features ............ -
Page 3: Before Use
1 Before use 1.1 General notes You must open the packaging carefully, to make sure that none of the accessories in the packaging fall on the floor and get broken. In general, microscopes should always be handled carefully because they are sensitive precision instruments. -
Page 4: Storage
Under no circumstances should you touch the integrated halogen bulbs either during operation or directly after use. These bulbs produce significant heat and therefore there is a risk that the user could be severely burnt. So before handling the bulbs, you must check that they have cooled down. -
Page 5: Maintenance And Cleaning
• Special cleaner for optical lenses • Special optical cleaning cloths • Bellows • Brush When handled correctly and checked regularly, the microscope should give many years of efficient service. Should repairs still be necessary, please contact your KERN dealer or our Technical Department. OBL-14-15-BA-e-2312... -
Page 6: Nomenclature
2 Nomenclature OBL-14-15-BA-e-2312... - Page 7 Rear view Carrying handle Adjusting ring torque Fine adjustment knob Main switch Coarse adjustment knob Fuse Power connection Adjusting ring torque Condenser Focus dial OBL-14-15-BA-e-2312...
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Page 8: Technical Data / Features
395x200x380 mm Packaging dimensions: 400x280x435 mm Net weight: 6,7 kg Gross weight: 8 kg Input voltage: AC 100-240V, 50-60Hz Output voltage DC 1,2-12V OBL 155: Output voltage DC 5V OBL 146 / OBL 156: Fuse OBL 155: 2A 5x20mm OBL-14-15-BA-e-2312... - Page 9 OBL-14-15-BA-e-2312...
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Page 10: Assembly
4 Assembly 4.1 Microscope head Inside the packaging the microscope head is already mounted but inclined towards the rear. In order to turn it to the front you must loosen the fixing screw on the tube connection point and after the turning fix it again with the screw. In case of removing the head completely from the housing, you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures. -
Page 11: Ph Condenser + Ph Slide
4.5 PH condenser + PH slide The condenser is firmly fixed onto a holding ring (condenser holder) underneath the microscope stage. There is the ability to adjust the height of the condenser, but not to centre it. We recommend that you use the coarse adjustment knob to bring the specimen stage to its uppermost position when you need to remove the condenser. -
Page 12: Pre-) Focussing
5.2 (Pre-) focussing When you are observing an object, you must have the correct distance to the objective to achieve a sharp image. In order to find this distance at the beginning (without other default settings of the microscope) place the objective with the lowest magnification in the beam path, look through the right eyepiece with the right eye and turn it slowly using the coarse adjustment knob (see illustration). -
Page 13: Adjusting The Interpupillary Distance
5.3 Adjusting the interpupillary distance With binocular viewing, the interpupillary distance must be adjusted accurately for each user, in order to achieve a clear image of the object. While you are looking through the eyepieces, use your hands to hold the righthand and lefthand tube housing firmly. -
Page 14: Adjusting The Magnification
5.5 Adjusting the magnification After prefocussing has been carried out using the objective with the lowest magnification (see section 5.2), you can then adjust the overall magnification using the nosepiece, as necessary. By turning the nosepiece you can bring any one of the four other objectives into the beam path. -
Page 15: Adjusting The Koehler Illumination
If, as with the devices in the KERN OBL-14 and OBL-15 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 16 4. Open the field diaphragm until it just disappears out of the field of view. If necessary, simply re-centre using the centring screws on the condenser holder. 5. Use the aperture diaphragm of the condenser to find the very best compromise between contrast and resolution for the microscopic image.
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Page 17: Using Eye Cups
5.7 Using eye cups The eye cups supplied with the microscope can basically be used at all times, as they screen out intrusive light, which is reflected from light sources from the environment onto the eyepiece, and the result is better image quality. But primarily, if eyepieces with a high eye point (particularly suitable for those who wear glasses) are used, then it may also be useful for users who don’t wear glasses, to fit the eye cups to the eyepieces. -
Page 18: Using Oil Immersion Objectives
5.8 Using oil immersion objectives The 100x objectives of the OBL-14 and OBL-15 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. -
Page 19: Phase Contrast Unit
5.9 Phase contrast unit Simple phase-contrast unit This consists of a simple PH condenser, a PH objective with a specific magnification (10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a centring telescope and a green filter. The standard outfit of the models of the series OBL-14 and OBL-15 always includes a 10x and 40x phase contrast unit. - Page 20 To use this, you need to position the desired PH objective in the beam path and furthermore to place the corresponding PH slide into the insertion point of the PH condenser. With the surface marked with the magnification information facing up, the PH slider is pushed into the insertion point on the PH condenser until stop.
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Page 21: Changing The Bulb
Grease and dust residue can have a negative effect on the light quality and service life. LED (OBL 146 / OBL 156) Before changing the bulb the device must be switched off and unplugged. To change the bulb, tip the device carefully to the back or side. When doing this, please make sure that all microscope components are firmly fixed. -
Page 22: Changing The Fuse
7 Changing the fuse (OBL 155) The fuse housing is on the rear of the microscope below the mains power supply socket. With the device switched off and unplugged, you can pull out the housing. When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse can be removed from its housing and be replaced with a new one. -
Page 23: Trouble Shooting
9 Trouble shooting Problem Possible causes The mains plug is not correctly plugged in There is no power at the socket The bulb does not light Defective bulb Defective fuse The bulb blows immediately The specified bulb or fuse has not been used The aperture diaphragm and/or field diaphragm are not opened wide enough The selector switch for the beam path is set... - Page 24 Problem Possible causes The aperture diaphragm is not opened wide enough The condenser is too low The objective does not belong to this microscope Blurred details The front lens of the objective is dirty An immersion object has been used without Bad image immersion oil Bad contrast...
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Page 25: Service
The device may only be opened by trained service engineers who have been authorised by KERN. 11 Disposal The packaging is made of environmentally-friendly materials, which you can dispose of at your local recycling centre. - Page 26 NOTES __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ _________________________________________________________ OBL-14-15-BA-e-2312...
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