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User instructions
Fluorescence microscope
KERN
OBN-14
Version 1.0
01/2015
OBN-14-BA-e-1510
KERN & Sohn GmbH
Ziegelei 1
D-72336 Balingen
E-mail: info@kern-sohn.com
OBN 141, OBN 147, OBN 148
Tel: +49-[0]7433- 9933-0
Fax: +49-[0]7433-9933-149
Internet: www.kern-sohn.com
Importør:
Impex Produkter AS
Gamle Drammensvei 107
1363 Høvik
www.impex.no
info@impex.no
Tel.: 22 32 77 20
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Summary of Contents for KERN OBN-14
- Page 1 KERN & Sohn GmbH Ziegelei 1 Tel: +49-[0]7433- 9933-0 D-72336 Balingen Fax: +49-[0]7433-9933-149 E-mail: info@kern-sohn.com Internet: www.kern-sohn.com User instructions Fluorescence microscope Importør: Impex Produkter AS KERN OBN-14 Gamle Drammensvei 107 1363 Høvik www.impex.no info@impex.no Tel.: 22 32 77 20 OBN 141, OBN 147, OBN 148 Version 1.0...
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Page 3: Table Of Contents
KERN OBN-14 Version 1.0 01/2015 User instructions Fluorescence microscope Table of contents Before use ..................3 General notes ..........................3 Notes on the electrical system ....................3 Storage ............................4 Maintenance and cleaning ....................... 5 Nomenclature ................7 Technical data / Features ............9 Assembly .................. -
Page 4: Before Use
Suitable replacement fuses are included with the delivery. When carrying out any procedures whereby you come into contact with the electrical system of the device, such as, for example, changing the bulb or fuse, only carry out these procedures when the power is disconnected. OBN-14-BA-e-1510... -
Page 5: Storage
The best way to store accessories which consist of optical elements, such as, for example, eyepieces and objectives, is in a dry box with desiccant. OBN-14-BA-e-1510... -
Page 6: Maintenance And Cleaning
Special cleaner for optical lenses Special optical cleaning cloths Bellows Brush When handled correctly and checked regularly, the microscope should give many years of efficient service. Should repairs still be necessary, please contact your KERN dealer or our Technical Department. OBN-14-BA-e-1510... - Page 7 NOTE: The Fluorescence microscopes of the series OBN-14 can be divided into two main components: Compound microscope (KERN Professional Line) Fluorescence reflected light unit The following user manual refers at first merely to the compound microscope. The disquisition on the fluorescence reflected light unit is carried out at the end of chapter 5.
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Page 8: Nomenclature
Microscope head Object holder Specimen Control dial stage X – Y axis Condenser Specimen stage Aperture adjustment Dimmer Condenser Centring screw Condenser Fine adjustment knob Field lens with Coarse Field diaphragm adjustment knob Centring screw Adjusting screw Condenser Condenser OBN-14-BA-e-1510... - Page 9 Rear view Carrying handle Coarse adjustme nt knob Fine adjustment knob Main switch Fuse Power connection Adjusting ring torque Condenser Focus dial OBN-14-BA-e-1510...
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Page 10: Technical Data / Features
Objectives: 4x / 10x / 20x / 40x / 100x Product dimensions: 970x345x440 mm Packaging dimensions: 530x220x490 mm Net weight: 17 kg Gross weight: 23 kg Input voltage: AC 100-240V, 50-60Hz Output voltage: DC 1.2-6V Fuse: 2A 5x20 mm OBN-14-BA-e-1510... - Page 11 OBN-14-BA-e-1510...
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Page 12: Assembly
4 Assembly OBN-14-BA-e-1510... -
Page 13: Microscope Head
A blue colour filter is already an integral part of the condenser. It is screwed onto the underside using a ring bracket. It can be removed at any time. In order to use suitable filters without this holder ring, these can simply be placed in the ring bracket of the field lens. OBN-14-BA-e-1510... -
Page 14: Condenser
(see illustration on the left). To move the sample into the beam path, you must use the adjustment wheels on the right of the angle table (see illustration on the right). You can position a total of two object holders at the same time. OBN-14-BA-e-1510... -
Page 15: Pre-) Focussing
Important: In order to avoid damaging to the focussing system, the left and right adjustment wheels for the coarse and fine adjustment knob must never be rotated at the same time in opposite directions. OBN-14-BA-e-1510... -
Page 16: Adjusting The Interpupillary Distance
3. Then look through the left eyepiece with the left eye and use the lefthand dioptre adjustment ring to focus the image. To do this, you just need to turn the ring in both directions (see illustration), to find out where the image is at its most focussed. OBN-14-BA-e-1510... -
Page 17: Adjusting The Magnification
If you have focussed the object to be observed for a specific magnification, then if you select the objective with the next greatest magnification, then the object will be slightly out of focus. Use the fine adjustment knob to make a slight adjustment and restore the focus. OBN-14-BA-e-1510... -
Page 18: Adjusting The Koehler Illumination
If, as with the devices in the KERN OBN-14 series, the lighting can be set in accordance with Koehler, the result is homogenous illumination of the slide and avoidance of disruptive stray light. - Page 19 5. Open the field diaphragm until it just disappears out of the field of view. If necessary, simply re-centre using the centring screws on the condenser holder. OBN-14-BA-e-1510...
- Page 20 If another magnification is selected afterwards, then the Koehler illumination does not have to be reset from scratch, only the aperture diaphragm and field diaphragm need to be adjusted as required. As a result you can always check whether the condenser needs to be re-centred. OBN-14-BA-e-1510...
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Page 21: Using Eye Cups
In order to maintain hygiene, we would therefore recommend that you clean the eye cups regularly (e.g. with a damp cloth). Eye cups High Eye Point eyepiece (identified by the glasses symbol) OBN-14-BA-e-1510... -
Page 22: Using Oil Immersion Objectives
5.8 Using oil immersion objectives The 100x objectives of the OBN-14 series are objectives which can be used with oil immersion (they are always marked with the word “OIL”). Using these generates a particularly high resolution for microscopic images. To use oil immersion correctly, please follow these steps. -
Page 23: Fluorescence Reflected Light Unit
The barrier filter finally eliminates all wave ranges out of the beam path not belonging to the observed fluorescence. Thus the resulting image is just developed by the fluorescent light emitted from the sample. OBN-14-BA-e-1510... - Page 24 Overview of wave lengths for excitation and emission per excitation filter Blue line: Wave length excitation Green line: Wave length emission OBN-14-BA-e-1510...
- Page 25 OBN-14-BA-e-1510...
- Page 26 OBN-14-BA-e-1510...
- Page 27 HBO/LED lamp housing Cover for FL module Power connection Fixing Allen screw for microscope head Screw for attaching the protective plate Main body Connection point for microscope housing Connection point for microscope head Allen wrench Fixing screw for lamp housing OBN-14-BA-e-1510...
- Page 28 Centring device for field diaphragm Screw for cover of lamp housing Control lever for field diaphragm Centring screw for lamp (vertical) Centring device for aperture diaphragm Centring screw for lamp (horizontal) Control lever for aperture diaphragm Control lever for illumination OBN-14-BA-e-1510...
- Page 29 Nomenclature (Power supply unit for HBO lamp) Basic data There are two different fluorescence reflected light units available for the microscopes of the series OBN-14. They have the following specifications. Article Illumination Excitation Centring Input voltage number filter objective power supply unit...
- Page 30 4. After placing the sample you can bring the needed objective into the beam path. 5. Choose the right position of the control wheel for the FL module. 6. When using a HBO lamp, now the control lever for the illumination can be pulled out. 7. Start the observation. OBN-14-BA-e-1510...
- Page 31 (This applies for 100W HBO lamps): o Ampere meter on the power supply unit As soon as 4.8 A are reached lamp replacement o Life time display on the power supply unit As soon as 100 h are reached lamp replacement OBN-14-BA-e-1510...
- Page 32 Impurities will benefit the risk of explosion during operation. If there should be nevertheless impurities, the lamp has to be cleaned. We recommend for this purpose to use a lint-free cloth soaked with an ether alcohol mixture (Proportion: 70/30). OBN-14-BA-e-1510...
- Page 33 The defective fuse can be removed from its housing and be replaced with a new one. After that, you just need to insert the fuse housing back into the insertion point next to the mains power supply socket. OBN-14-BA-e-1510...
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Page 34: Changing The Bulb
When doing this, it is helpful to use a screwdriver or similar tool. The defective fuse can be removed from its housing and be replaced with a new one. After that, you just need to insert the fuse housing back into the insertion point below the mains power supply socket. OBN-14-BA-e-1510... -
Page 35: Using Optional Accessories
The aperture setting of the condenser must be in position PH. For its starting position, the polariser must be turned to the position in which you can see the highest level of darkness in the field of view (without object slide). OBN-14-BA-e-1510... -
Page 36: Camera Connection
8.2 Camera connection Due to the trinocular tube, which is a standard fitting for the whole OBN-14 series, it is possible to connect microscope cameras to the device, in order to digitally record images or sequences of images of an object being observed. -
Page 37: Phase-Contrast Units
To use this, you need to replace the standard condenser of the microscope with the PH condenser. One of the objectives in the nosepiece is also replaced with the PH objective and this is positioned in the beam path. OBN-14-BA-e-1510... - Page 38 The position of the ring in the objectives cannot be altered, that of the rings in the condenser disc however can be. OBN-14-BA-e-1510...
- Page 39 Depending on the preferences of the user, using a green filter can produce a more effective and pleasant image. To achieve this, the green filter must be screwed onto the underside of the PH condenser. OBN-14-BA-e-1510...
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Page 40: Trouble Shooting
Dioptre setting has not been carried out correctly The field of view of one eye does not Different eyepieces are used for the match that of the other eye righthand and lefthand side The eyes are not used to using a microscope OBN-14-BA-e-1510... - Page 41 The stage moves down on its own The rotational resistance brake is not tight The fine adjustment knob moves on its enough When you move the table, the image The stage was not correctly fitted becomes blurred OBN-14-BA-e-1510...
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Page 42: Service
The device may only be opened by trained service engineers who have been authorised by KERN. 11 Disposal The packaging is made of environmentally-friendly materials, which you can dispose of at your local recycling centre. - Page 43 NOTES __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ __________________________________________________________ _________________________________________________________ OBN-14-BA-e-1510...