Using Oil Immersion Objectives - KERN OLE-1 User Instructions

5.8 Using oil immersion objectives

The 100x objectives of the OLE-1 and OLF-1 series are objectives which can be
used with oil immersion (they are always marked with the word "OIL"). Using these
generates a particularly high resolution for microscopic images.
To use oil immersion correctly, please follow these steps.
1. Put an object holder of glass between the specimen stage and the observed
object.
2. Raise the specimen stage and position the 100x objective in the beam path.
3. Put a drop of oil on top of the objective lens.
4. Bring the specimen stage or object slide to the objective very slowly until there is
slight contact.
5. Observe the object.
Please prevent to use too much oil on the objective lens, because there is the risk
that the oil running down on the objective.
The object slide and objective must not be pressed against each other. The oil
constitutes the contact layer.
If the contact is made too jerky, there is a chance that existing air bubbles in the oil
cannot escape. This would have a negative impact on image clarity.
After use or before changing the slide, any components which have been in contact
with the oil must be cleaned thoroughly. See also 1.4 Maintenance and cleaning.
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