PSI Polypen Aqua Instruction Manual
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PSI Polypen Aqua Instruction Manual

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  • Page 2 This document and its parts can be copied or provided to a third party only with the express permission of PSI. The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.

  • Page 3: Table Of Contents

    ABLE OF ONTENT Information before using PolyPen-Aqua device ....................4 General Description ............................5 Technical Specification ..............................6 Device Description ............................7 List of equipment and customer information......................9 Care and maintenance ..............................9 Getting started ............................... 10 Calibration ................................10 Measurement .................................11 Menu description ............................12 PC communication ............................

  • Page 4: Information Before Using Polypen-Aqua Device

    NFORMATION BEFORE USING QUA DEVICE Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide. Always follow corresponding manuals while working with the PolyPen-Aqua device or doing the maintenance.

  • Page 5: General Description

    ENERAL ESCRIPTION PolyPen-Aqua PA 210 is a portable, handheld and reasonably priced spectrophotometer intended for measuring spectra in suspensions. It can be used favorably in a wide range of applications – biotechnology, limnology, ecology, molecular biology, chemistry, forensic science etc. The device is convenient both for standard laboratory work and for field work, well using its inbuilt GPS unit. The PolyPen- Aqua measures absorbance and transmittance spectra based on a single beam method;...

  • Page 6: Technical Specification

    2.1 T ECHNICAL PECIFICATION Spectral range 340 nm - 790 nm Spectral response half width 8 nm Spectral straylight -30 dB Scanning speed About 100 ms High gain: 1:4300 Dynamic range Low gain: 1:13000 Size of aperture 7 mm Light source Xenon incandescent lamp Light source spectral range 340-790 nm...

  • Page 7: Device Description

    EVICE ESCRIPTION Fig. 1 Device description. Page | 7...
  • Page 8 Cuvette slot The sample is measured in a plastic cuvette inserted into an optical head and covered by a top lid. Colour touch screen display PolyPen is operated via a touch screen by using a supplied stylus. The software operation instructions are available on page 19. No sharp objects should be used when operating the PolyPen-Aqua via touch screen.

  • Page 9: List Of Equipment And Customer Information

    Do not keep the battery at full charge for long periods of time. Allow for it to discharge. • High temperatures shortens battery life. • If the battery can no longer be charged, please contact PSI for replacement battery and installation instructions. Page | 9...

  • Page 10: Getting Started

    ETTING STARTED To switch ON the PolyPen-Aqua, press the Power button until the device logo shows up. Follow next steps to perform the measurements. In case the device battery is not charged, connect the PolyPen-Aqua with USB cable via USB port to the PC and charge the battery. The measurements with the PolyPen-Aqua cannot be made until the calibration is performed.

  • Page 11: Measurement

    Fig. 3 Measure icon. Please remember or mark the orientation of the cuvette in the device. For repeated measurements it is recommended to position the cuvette always in the same orientation in the PA 210 cuvette holder. 4.2 M EASUREMENT Besides the whole spectrum information, the PolyPen-Aqua incorporates pre-defined formulas for calculation of optical density directly in its software.

  • Page 12: Menu Description

    ENU DESCRIPTION Device • Calibrate – calibration can be run any time by pressing Device > Calibrate after inserting cuvette with clear media. (Fig. 5). Fig. 5 Calibration of the device. Data • Browse – displays data browse dialog box. The user can browse the list of stored data, select the set of data files and view the light spectra in Transmittance or Absorbance mode (Options >...
  • Page 13 Options • Settings – function is used to set various variables for the light measurements and for the device settings. Indexes - Select indexes to be calculated and displayed on the main screen (Fig. 7). Fig. 7 Settings - Indexes. Optical density indices –...
  • Page 14 Fig. 8 Settings - Graph. Time - Set the actual time and date (all data files are stored by time and date signature). To change time, touch on one of the values and adjust it using the arrows (Fig. 9). Fig.
  • Page 15 Fig. 10 Settings - LCD. Device ▪ Average – Average function is used for adjusting the number of scans for each reading. Averaging of more scans results in a higher signal-to-noise ratio but increases the time required for each reading that appears on the screen.

  • Page 16: Pc Communication

    6 PC COMMUNICATION 6.1 USB CONNECTION PolyPen-Aqua comes with the USB cable that is required for charging of the Li-ion battery and can also be used for data transfer to the PC after completion of measurements. To connect the USB cable with the PolyPen-Aqua device follow the picture instructions below. Please note that a lock in system is used to secure the USB cable to the PolyPen-Aqua and extreme caution has to be used when setting up this connection to avoid damage to the cable pins.
  • Page 17 Navigate through System and security, System to Device manager (Fig. 14) Fig. 14 System window. • Connect the PolyPen to the PC. You should see that PSI USB Device appears in the list. Right click on it and select Update Driver Software…(Fig. 15) Fig. 15 PSI USB device.
  • Page 18 Installation of the driver is now complete (Fig. 17) Fig. 17 Complete installation. In case this driver installation is not successful the driver may be downloaded directly from PSI websites www.psi.cz. Once the device is properly connected to the computer, the indicating icon appears in upper right corner on the PolyPen display (Fig.

  • Page 19: Spectrapen Software

    PECTRA EN SOFTWARE 7.1 S OFTWARE INSTALLATION Copy the SpectraPen software provided on the USB flash disk to your computer and launch the SpectraPen program. To connect and recognize the PolyPen-Aqua device in the SpectraPen software, proceeds first with the registration of the SpectraPen software (Fig.
  • Page 20 Fig. 21 PolyPen-Aqua connect to the SW. Fig. 22 Connection failed. Page | 20...

  • Page 21: Software Menu

    7.2 S OFTWARE MENU MENU: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format. Export to JSON Exports data in JavaScript Object Notation. Close Closes the current experiment. Close All Closes all open experiments.

  • Page 22: Data Transfer And Visualization

    7.3 D ATA TRANSFER AND VISUALIZATION After completion of measurement PolyPen-Aqua may be connected to PC for data download and further analysis. Launch the SpectraPen software on the computer and Connect the device. Go to Device> Connect. To transfer your data from the PolyPen-Aqua device to your PC use the Download function. Select icon on left side of screen or go to File and select Load if you want to process data stored on PC.
  • Page 23 Fig. 24 Option for list of data. In the graph marker feature is available, which enables display of the numeric values for wavelength and transmittance or absorbance for the selected wavelength of the scan (Fig. 25). Use the mouse to select the given point. In top right corner of the graph (green rectangle) is displayed exact value for the selected point on the x-axis and y-axis.
  • Page 24 To zoom in on the data displayed in the graph select an area of the displayed graph. To reverse these steps and return to original display use minus icon in the corners of the zoom area marked with green rectangles in Fig. 26. Fig.
  • Page 25 Fig. 28 Transmittance – reflectance indices. 11. To Save the experiment select File>Save. All data stored in the device memory will be saved irrespective of the data selection in the SpectraPen software. The file will be stored as .spec. Spec files stores all Transmittance and numeric data. 12.

  • Page 26: Online Control

    7.4 O NLINE CONTROL Menu Online Control This function can be used for remote - online control of the PolyPen-Aqua device after connection with the PC. Online control enables to make changes in device setting and also perform remote measurement using PC. Select: Menu >...

  • Page 27: Firmware Update

    Settings (Fig. 33) Here the following functions may be set up for remote operation of the PolyPen-Aqua: • Time synchronization of the device and PC (Fig. 33 - 1) • GPS built-in module activation (Fig. 33 – 2; more information about the GPS module in chapter 8) •...

  • Page 28: Gps Module

    8 GPS MODULE PolyPen-Aqua device has integrated GPS module which may be turned on during the measurement for mapping of the collected data to specific field position. When GPS module is turned on, the map coordinates will be automatically saved with all collected data and will be downloaded during data download.
  • Page 29 Fig. 37 Data with GPS coordinates. Page | 29...

  • Page 30: Warranty Terms And Conditions

    The customer is responsible for shipping and insurance charges (for the full product value) to PSI. The manufacturer is responsible for shipping and insurance on return of the instrument to the customer. •...

  • Page 31: Appendix

    11 A PPENDIX 11.1 P ROGRAMMING CUSTOM INDEX IN The SpectraPen software enables programming custom indexes, which can be used for wide range of calculation based on the measured spectrum. Go to the main SpectraPen folder in your PC (Usually in Program Files). Open the file Config >...

  • Page 32: Function Description

    Fig. 39 Downloaded data with newly defined index. 11.1.1 F UNCTION DESCRIPTION Different mathematical functions can be used in SpectraPen software syntax to create new custom formulas. min, max - min(value1, value2) value1 - number, variable, function value2 – number, variable, function - only one value can be function! min(array) array –...
  • Page 33 example1: ln(5) example2: ln(Transmitance[760nm]) example3: ln(max(Transmitance[550nm], Transmitace[480nm])) example4: ln((5+4)*4) __________________________________________________________________________ – the logarithm of specified number in a specified base. logB(value) B – base - number value – number, variable, function example1: log2(5) example2: log5(Transmitance[760nm]) example3: log10(max(Transmitance[550nm], Transmitance[480nm])) example4: log10((5+4)*4) __________________________________________________________________________ sqrt –...
  • Page 34 function_values – input values for integral compute from,to – limit values example1: integral(IrradianceL, 360nm, 700nm) example2: integral(IrradianceE, 360nm, 700nm) * IrradianceE[450] Page | 34...

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