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PSI FluorPen FP 110 Manual And User Manual

Portable, battery-powered fluorometer
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FluorPen FP 110
PAR-FluorPen FP 110
Monitoring Pen MP 100
Manual and User Guide
Please read this manual before operating this product
PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic
FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz

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  • Page 1 PAR-FluorPen FP 110 Monitoring Pen MP 100 Manual and User Guide Please read this manual before operating this product PSI, spol. s r. o., Drásov 470, 664 24 Drásov, Czech Republic FAX: +420 511 440 901, TEL: +420 511 440 011, www.psi.cz...
  • Page 2 © PSI (Photon Systems Instruments), spol. s r. o. (hereinafter PSI), 2018 This document and its parts can be copied or provided to a third party only with the express permission of PSI. The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.
  • Page 3 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 4: Table Of Contents

    © PSI (Photon Systems Instruments), spol. s r. o. TABLE OF CONTENTS Information Before Using FluorPen Device ........................6 Technical Specification ..............................7 General Information ............................... 9 Device description ............................... 12 List of equipment ................................13 Care and Maintenance ..............................14 Principle of Measurement ............................
  • Page 5 © PSI (Photon Systems Instruments), spol. s r. o. 12.2 Data Download ..............................51 Warranty Terms and Conditions ..........................52 Troubleshooting and Customer Support ......................... 53 Appendix .................................. 54 15.1 Battery Pack for Monitoring Pen ......................... 54 15.1.1 Standard Battery Pack ..........................54 15.1.2...

  • Page 6: Information Before Using Fluorpen Device

    © PSI (Photon Systems Instruments), spol. s r. o. 1 INFORMATION BEFORE USING FLUORPEN DEVICE Read this manual carefully before operating the device. If you are not sure about anything in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide.

  • Page 7: Technical Specification

    © PSI (Photon Systems Instruments), spol. s r. o. 2 TECHNICAL SPECIFICATION Measured / Calculated Parameters: ‘, QY, NPQ 1, NPQ 2, NPQ 3, OJIP, LC 1, LC 2, LC 3, PAR (measured as PPFD)* Cosine correction of PAR sensor*: Cosine corrected up to 80º...
  • Page 8 © PSI (Photon Systems Instruments), spol. s r. o. * Only in PAR-FluorPen FP 110. ** Windows is a registered trademark of Microsoft Corporation.

  • Page 9: General Information

    FluorPen is also an invaluable tool for large plant- screening programs. PAR-FluorPen FP 110 includes all features of the FluorPen FP 110, i.e., it measures chlorophyll fluorescence parameters Ft, QY, NPQ, OJIP, and LC (QY). Besides that, the PAR-FluorPen incorporates an integrated Light Meter for direct digital readouts of Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm, the span in which plants use energy during photosynthesis.
  • Page 10 Measures in addition to standard fluorescence protocols also Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm. The “X“ version is mounted with custom-made leaf-clip. Fig. 1a) PAR-FluorPen FP 110/S. b) PAR-FluorPen FP 110/D. c) FluorPen FP 110/P. Monitoring Pen versions: Monitoring Pen MP 100-E (Fig.
  • Page 11 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 2a) Monitoring Pen MP 100-E. b) Monitoring Pen MP 100-A.

  • Page 12: Device Description

    © PSI (Photon Systems Instruments), spol. s r. o. 3. 1 D EV IC E D E S CRI P TI ON Fig. 3 Device description.

  • Page 13: List Of Equipment

    (USB driver folder). For more information go to If any item is missing, please, contact PSI. Also check the carton for any visible external damage. If you find any damage, notify the carrier and PSI immediately. The carton and all packing materials should be retained for inspection by the carrier or insurer.

  • Page 14: Care And Maintenance

    © PSI (Photon Systems Instruments), spol. s r. o. 5 CARE AND MAINTENANCE • Never submerge the device in water! (except Monitoring Pen MP 100-A) • The device should not come in contact with any organic solvents, strong acids or bases.

  • Page 15: Principle Of Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 6 PRINCIPLE OF MEASUREMENT FluorPen is a fluorometer which can measure different fluorescence parameters of plants. It is equipped with a blue LED emitter (455 nm), optically filtered and precisely focused to deliver light intensities of up to 3,000 µmol.m measured plant tissue (Fig.
  • Page 16 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 5 Chlorophyll fluorescence. FluorPen measures: - Instantaneous Chlorophyll Fluorescence is equivalent to F if the sample is dark-adapted. QY - Quantum Yield QY is a measure of the Photosystem II efficiency. QY is equivalent to F in dark-adapted samples and to F light-adapted samples.

  • Page 17: How To Get Started

    © PSI (Photon Systems Instruments), spol. s r. o. 7 HOW TO GET STARTED For more detailed information on particular steps of FluorPen operation please refer to chapter 8. The device can work on Li-Ion battery or is charged via USB cable from PC or grid. Monitoring Pen can be charged also from battery pack (see more in chapter 15.1)
  • Page 18 © PSI (Photon Systems Instruments), spol. s r. o. Setting of optimal intensities of pulses: Flash pulse setting The optimum value of Flash pulse you can identify with QY measurement. Before QY measurement is recommended to set the intensity of Super pulse on 80 %.

  • Page 19: Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 7 OJIP measurement performed with different intensities of Super pulse. Actinic pulse setting Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application.

  • Page 20: Protocols Explanation

    © PSI (Photon Systems Instruments), spol. s r. o. 7. 3 PR OT OC O LS EXP L AN A TI O N 7.3.1 OJIP PROTOCOL The FluorPen device offers the possibility to capture rapid fluorescence transient – OJIP, which occurs during exposure of photosynthesizing organisms to high irradiance.

  • Page 21: Non-Photochemical Quenching (Npq) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. Formulas Derived From: R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P.
  • Page 22 © PSI (Photon Systems Instruments), spol. s r. o. L - indicates light adapted parameters; D - refers to dark recovery phase after switching of the actinic illumination; n - represents a sequential number of light phase; ss - steady state Calculated as (F –...
  • Page 23 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 8 NPQ Protocol.

  • Page 24: Light Curve (Lc) Protocol

    © PSI (Photon Systems Instruments), spol. s r. o. 7.3.3 LIGHT CURVE (LC) PROTOCOL The protocol called Light Curve (LC) was designed to acquire parameters for construction of Light Response Curve relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the sample exposed to a stepwise increase of light intensity.
  • Page 25 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 9 LC1 Protocol.
  • Page 26 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 10 LC2 Protocol.

  • Page 27: Multiple Measurement

    © PSI (Photon Systems Instruments), spol. s r. o. 7. 4 M UL T IP L E M E A S UR EM E NT Except single measurement you can perform multiple measurements. In this mode FluorPen repeats measurements according to parameters, which you have set in Settings > Multi (see Menu tree, page 28) Multi type –...

  • Page 28: Control Menu Tree

    © PSI (Photon Systems Instruments), spol. s r. o. 8 CONTROL MENU TREE The next pages show the structure of the operation scheme, which includes the Main Menu, first-level Sub-Menus and second-level Sub-Menus. • The blue color represents the Main Menu and its Options.
  • Page 29 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 30 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 31 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 32 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 33 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 34 © PSI (Photon Systems Instruments), spol. s r. o.
  • Page 35 © PSI (Photon Systems Instruments), spol. s r. o.

  • Page 36: Usb Connection

    If you check the Device Manager in Windows you should see the USB serial port in the device tree. In case of missing driver you may download it from PSI websites. When the driver is installed correctly you should be able to connect to the device in the FluorPen software menu Setup >...

  • Page 37: Bluetooth Connection

    © PSI (Photon Systems Instruments), spol. s r. o. 10 BLUETOOTH CONNECTION Before you set up the Bluetooth connection between the FluorPen and your PC, make sure you have these components: 1. Bluetooth enabled PC The PC with which you connect must have Bluetooth wireless technology, either built-in or through a Bluetooth card.
  • Page 38 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 12 Start Bluetooth Application. 3. Opening the Add Bluetooth Device Application • Select: “Add a device” to start searching new Bluetooth device. Be sure that the FluorPen is in discoverable mode (see step 1).
  • Page 39 © PSI (Photon Systems Instruments), spol. s r. o. 5. Starting the Pairing Process This step is different for old and new version of Bluetooth module. Old version: Your Bluetooth Pairing Code is: 0000 • Select: “Enter the device’s pairing code”.
  • Page 40 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 17 Finishing. On computer run the program FluorPen 1.1. For more information about FluorPen software see chapter 11.

  • Page 41: Fluorpen Software

    © PSI (Photon Systems Instruments), spol. s r. o. 11 FLUORPEN SOFTWARE 11 .1 SO F TW A R E IN S T AL L AT IO N 1. Save the FluorPen software provided on the USB flash disk to your computer and launch the FluorPen program.

  • Page 42: Menu And Icon Explanation

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 19 Connecting FluorPen device with Software. 11 .2 M EN U AN D IC ON E XP L A N A TI ON 11.2.1 MAIN MENU MENU: File Load Loads previously saved data files.

  • Page 43: Menu Settings

    © PSI (Photon Systems Instruments), spol. s r. o. MENU: Setup Device ID Detects the connected device. Update Firmware Used for firmware updates. Settings Used for modification of the program settings. MENU: Help About Offers basic information about the program.

  • Page 44: Menu Online Control

    © PSI (Photon Systems Instruments), spol. s r. o. Data – GPS Visible This option is active only in older FluorPen version FP 100 and Monitoring Pen MP 100. In new versions of FP 110 the GPS data are automatically downloaded and paired with protocol measurements.
  • Page 45 Set the FluorPen time a date. You can also synchronize time of FluorPen device with computer time. Online Control – Protocols Choose the protocol for measuring. FluorPen device start to measure. After measuring you can download data. Measuring of PAR is active only in the PAR-FluorPen FP 110.

  • Page 46: Data Transfer And Visualization

    © PSI (Photon Systems Instruments), spol. s r. o. 11 .3 D AT A TR AN S F ER AN D VI SU A LI ZA T IO N 1. Perform a measurement with your FluorPen. In Fig. 20 we did an OJIP and NPQ measurement.
  • Page 47 © PSI (Photon Systems Instruments), spol. s r. o. 4. To visualize measurement in the graphic mode, click the Graph field in the bottom bar. 5. The colored Graph of measured data appears (Fig. 21). Fig. 21 Graphic visualization of experiment.

  • Page 48: Firmware Update

    © PSI (Photon Systems Instruments), spol. s r. o. 11 .4 FIR M W A R E U P D AT E All data in the FluorPen memory are erased during the firmware update! Before starting any firmware update, export all your data from the FluorPen memory into your computer! 1.
  • Page 49 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 25 Select .bxn file. 4. Finishing Upload • Select: OK to start uploading of the update (Fig. 26). Fig. 26 Data loss warning. • The bottom bar indicates the upload progress (Fig. 27).

  • Page 50: Gps Module

    © PSI (Photon Systems Instruments), spol. s r. o. 12 GPS MODULE FluorPen device has integrated GPS module which can be turned on during the measurement and the GPS coordinated will be added to the downloaded data. For proper GPS reading, the time in your FluorPen and in your computer must be synchronized.

  • Page 51: Data Download

    © PSI (Photon Systems Instruments), spol. s r. o. 12 .2 D AT A D O WN LO A D 1. Enabling Communication: • Switch on the computer and the FluorPen device. Set your computer to FluorPen communication: enable Bluetooth or connect to USB port.

  • Page 52: Warranty Terms And Conditions

    The customer is responsible for shipping and insurance charges (for the full product value) to PSI. The manufacturer is responsible for shipping and insurance on return of the instrument to the customer.

  • Page 53: Troubleshooting And Customer Support

    © PSI (Photon Systems Instruments), spol. s r. o. 14 TROUBLESHOOTING AND CUSTOMER SUPPORT In case of troubles and for customer support, please, visit on our websites, write to support@psi.cz or contact your local distributor.

  • Page 54: Appendix

    © PSI (Photon Systems Instruments), spol. s r. o. 15 APPENDIX 15 .1 B AT T ER Y P AC K FO R M O N IT OR IN G P E N Battery pack serves as external battery source for Monitoring pen devices. The external battery ensures powering of devices during long-term experiments.

  • Page 55: Extended Temperature Range Battery Pack

    © PSI (Photon Systems Instruments), spol. s r. o. Fig. 32 Connectors of Standard Battery Pack. Battery exchange: 1. Unscrew 4 screws and remove the battery pack cover (Fig. 33a). 2. Disconnect the internal battery from the cables. 3. Place new battery into the pack, connect it with the cables – red cable with red marked connector and black cable with black marked connector (Fig.
  • Page 56 © PSI (Photon Systems Instruments), spol. s r. o. Extended temperature range battery pack cannot be recharged. Spare battery is offered as additional accessory. Fig. 34 Monitoring Pen MP 100-A charged via Extended Temperature Range Battery Pack. Connectors of Battery Pack: Device Serves for connection between Monitoring Pen and the battery.
  • Page 57 © PSI (Photon Systems Instruments), spol. s r. o. Fig. 35 Battery exchange of Extended Temperature Range Battery Pack.

This manual is also suitable for:

Par-fluorpen fp 110Monitoring pen mp 100

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