PSI Polypen Aqua Manual
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PSI Polypen Aqua Manual

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Summary of Contents for PSI Polypen Aqua

  • Page 1 Page | 1...
  • Page 2 Manual Version: 2021/01 © PSI (Photon Systems Instruments), spol. s r.o. www.psi.cz This document and its parts can be copied or provided to a third party only with the express permission of PSI. The contents of this manual have been verified to correspond to the specifications of the device.

  • Page 3: Table Of Contents

    ABLE OF ONTENT Warnings and Safety Precautions ................4 Technical specifications ..................... 5 General Information ....................6 Components of the PolyPen-Aqua Device ..............7 Description of the PolyPen-Aqua Device ..............8 Care and Maintenance ....................9 Operation Instruction ....................10 Calibration ........................

  • Page 4: Warnings And Safety Precautions

    ARNINGS AND AFETY RECAUTIONS Read this manual carefully before operating the device. If you are not sure about anything in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide.

  • Page 5: Technical Specifications

    ECHNICAL PECIFICATIONS Spectrum measurement Spectral range 340 nm - 790 nm Spectral response half width 8 nm Spectral straylight -30 dB Scanning speed About 100 ms High gain: 1:4,300 Dynamic range Low gain: 1:13,000 Size of aperture 7 mm Light source Type Xenon incandescent lamp Spectral range...

  • Page 6: General Information

    ENERAL NFORMATION PolyPen-Aqua PA 210 is a portable, handheld and reasonably priced spectrophotometer intended for measuring spectra in suspensions. It can be used favorably in a wide range of applications – biotechnology, limnology, ecology, molecular biology, chemistry, forensic science etc. The device is convenient both for standard laboratory work and for field work, well using its inbuilt GPS unit.

  • Page 7: Components Of The Polypen-Aqua Device

    If you find any damage, notify the carrier and the manufacturer immediately. The carton and all packing materials should be retained for inspection by the carrier or insurer. For customer support, please write to: support@psi.cz Page | 7...

  • Page 8: Description Of The Polypen-Aqua Device

    ESCRIPTION OF THE EVICE PolyPen-Aqua is a compact, durable and lightweight device operated by single Turn ON/OFF button and touch screen (Fig. 1 PolyPen-Aqua device.Fig. 1). It features an innovative user interface with an integral computer, a full color touch screen display and an in-built light source. The sample is measured in a plastic cuvette inserted into an optical head and covered by a top lid.

  • Page 9: Care And Maintenance

    ARE AND AINTENANCE • Never submerge the device in water! • The device should not come in contact with any organic solvents, strong acids or bases. • Battery life is approximately 48 hours when the PolyPen-Aqua is operated continuously. • Use the provided stylus for the touch-screen operation.

  • Page 10: Operation Instruction

    PERATION NSTRUCTION This chapter provides instructions on an initial set-up operation of the PolyPen-Aqua PA 210 device as well as detailed, step-by-step description of a measurement procedure. To switch the PolyPen-Aqua ON, press the POWER BUTTON until the device logo shows up. Follow next steps to perform the measurements.
  • Page 11 • Calibration is automatically stored into the device memory. • After the calibration step is performed, Main window icon Calibrate will change into Measure (Fig. 3). • Remember that the calibration is done for the particular cuvette. If you want to use another cuvette, you should make the calibration with this one.

  • Page 12: Measurement

    7.2 Measurement • Fill the cuvette with a sample of algae or cyanobacteria and close the cuvette with the stopper. Minimal volume of sample is 2 ml. • Put cuvette with sample to PolyPen-Aqua device. • Close the cover. • In the main window select >...

  • Page 13: Main Menu

    7.3 Main Menu In this chapter the settings of the optimal device parameters and features of displayed data are described. Device Calibrate – calibration can be run any time by pressing Device > Calibrate after inserting cuvette with clear media (Fig. 5). Fig.

  • Page 14: Options

    Fig. 6 Menu Data, Browse data. Options Settings – function is used to set various variables for the light measurements and for the device settings (Fig. 7). Fig. 7 Settings > Indexes. Page | 14...
  • Page 15 • Indexes - Select indexes to be calculated and displayed on the main screen (Fig. 8). Optical density indices – values are calculated as OD = -log(I/I - where is the irradiance that is transmitted through the cuvette filled with clear medium. This quantity must be measured as the reference.
  • Page 16 Absorbance – switches to absorbance mode of spectrum visualization, the raw spectral data are calculated according to the formula: A = log(I /I). Wavelength range – defines the range of wavelengths considered for light scope and light meter measurements. Desired wavelength range can be adjusted by selecting the wavelength and by using the arrows up and down.
  • Page 17 Fig. 10 Time setting. Fig. 11 Display intensity and time-out settings. Page | 17...
  • Page 18 • Device – enables the GPS module. The GPS coordinates cannot be displayed at the device display, but they are exported together with data and visualized in SpectraPen software (Fig. 12, Fig. 13). Average – Average function is used for adjusting the number of scans for each reading. Averaging of more scans results in a higher signal-to-noise ratio but increases the time required for each reading that appears on the screen.

  • Page 19: Spectrapen Software

    2. Navigate through System and security and System Device manager. 3. Connect the PolyPen-Aqua to the PC. You should see that PSI USB Device appears in the list. Right click on it and select Update Driver Software… Page | 19...
  • Page 20 4. Click Browse my computer for driver software and select Driver folder on the PolyPen- Aqua installation disk. Allow the installation even the warning table appears. 5. Installation of the driver is now complete. Page | 20...

  • Page 21: Software Installation

    8.2 Software Installation 1. Save the SpectraPen software provided on the USB flash disk to your computer and launch the SpectraPen program (for PolyPen-Aqua is the same software like for SpectraPen). 2. To connect and recognize your PolyPen-Aqua device in the SpectraPen software proceed with the registration of your SpectraPen software first.
  • Page 22 Please follow the next steps on Fig. 16: Outlet connector on PolyPen-Aqua device. Inlet part on the USB cable. C) – E) Position the cable horizontally, plug in the inlet and screw the securing screw. Correct connection of the USB cable and Pen device. Fig.
  • Page 23 Not connected will occur in the bottom left corner (Fig. 18). Repeat the entire procedure again first and if it does not solve the problem please contact our customer support at support@psi.cz. Fig. 18 Failed PolyPen-Aqua connection Page | 23...

  • Page 24: Spectrapen Software Menu

    8.3 SpectraPen Software Menu Menu: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format. Export JSON Exports data in JavaScript Object Notation. Close Closes the current experiment. Close All Closes all running experiments. Exit Exits the program.

  • Page 25: Data Transfer And Visualization

    Menu: Help Registration Used for the SpectraPen software registration. About Offers basic information about the SpectraPen application. Online Commands Online commands description. 8.4 Data Transfer and Visualization To download, visualize and further process of the measured data, please follow the next instructions: 1.
  • Page 26 • Transmittance: The measured spectral data are normalized to clear medium calibration by the linear calculation: T = I/I , where is the irradiance transmitted through the cuvette with algal or cyanobacterial suspension and is the irradiance that is transmitted through the cuvette filled with clear medium. Fig.
  • Page 27 Fig. 21 Transmittance 8. Draw rectangle by left mouse click around the area you would like to zoom in (Fig. 23). If you want to reverse these steps and return to original display, use minus icon in the corners of the zoom area as depicted by green arrows in Fig. 23. Fig.
  • Page 28 Fig. 23 Zoom feature 9. Go to Absorbance or Transmittance bookmark to view the measured data. 10. To view the spectral data in parameter values ( ) click to button with two arrows (Fig. 21). If you want to calculate your own index, you can do it from the raw data in Excel or you can add this formula to the Config/Formulas.txt in the PolyPen-Aqua program folder.
  • Page 29 • Spectrum Transmittance – transmittance data for all measurements are exported. The transmittance data are normalized to clear medium calibration. • Spectrum Absorbance – absorbance data for all measurement are exported. Data in absorbance mode are normalized to clear medium calibration by logarithmic calculation.

  • Page 30: Online Control

    Fig. 26 Export – Computed data selection and choosing of exported values 8.5 Online Control Online control serves for operation of the PolyPen-Aqua device in SpectraPen software after the connection with PC. The measurement, changes in settings and measurement repetition can be set through online control.
  • Page 31 Fig. 27 Setting of reflectance or absorbance measurements in online control Settings • Time synchronization with computer time (Fig. 28-1). Device > Online Control > Settings > Sync > OK • GPS – After GPS module is enabled, it begins acquiring satellite signals. Exported data then has GPS coordinates mark (Fig.

  • Page 32: Firmware Update

    8.6 Firmware Update All data in the PolyPen-Aqua memory are erased during the firmware update! Before starting any firmware update, export all your data from the PolyPen- Aqua memory into your computer! 1. Starting Update • Select: Device > Update Firmware 2.

  • Page 33: Gps Module

    9 GPS M ODULE PolyPen-Aqua device has integrated GPS module which can be turned on during the measurement and the GPS coordinated will be added to the exported data in *txt file. 9.1 GPS / PolyPen-Aqua Operation Step 1: Setting Time Check the time setting: Options >...
  • Page 34 Fig. 32 GPS icon Step 3: Measurement Provide the calibration of the device (if not done previously) and measure the reflectance indexes and scopes of your sample. Step 4: Export the data with GPS coordinates Export the computed data from the SpectraPen software. All the measured data will have GPS coordinate mark (Fig.

  • Page 35: How Can I Program My Own Index In The Polypen-Aqua Pa 210

    10 H ROGRAM NDEX PA 210 Two possibilities exist to define a custom index: 1. Offline calculation of the desired index from raw spectral data in Excel (independently on SpectraPen software). 2. Adding the custom formula to the list of pre-defined parameters in the SpectraPen software.

  • Page 36: Function Description

    Fig. 35 Downloaded data with new index 10.1 Function Description It is possible to use different mathematical functions for definition of new indices: min, max min(value1, value2) value1 - number, variable, function value2 – number, variable, function - only one value can be function! min(array) array –...
  • Page 37 example1: log2(5) example2: log5(Transmitance[760nm]) example3: log10(max(Transmitance[550nm], Transmitance[480nm])) example4: log10((5+4)*4) __________________________________________________________________________ sqrt – the square root of a specified number sqrt(value) value – number, variable, function example1: sqrt(5) example2: sqrt(Transmitance[760nm]) example3: sqrt(max(Transmitance[550nm], Transmitance[480nm])) example4: sqrt(((5+4)*4) + 6) ____________________________________________________________________________ - spedified number raised to the specified power value^power value –...

  • Page 38: Warranty Terms And Conditions

    The customer is responsible for shipping and insurance charges (for the full product value) to PSI. The manufacturer is responsible for shipping and insurance on return of the instrument to the customer.

  • Page 39: Troubleshooting And Customer Support

    12 T ROUBLESHOOTING AND USTOMER UPPORT In case of troubles and for customer support, please, visit on our websites, write to support@psi.cz or contact your local distributor. Page | 39...

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