1. Manuals
  2. Brands
  3. PSI Manuals
  4. Measuring Instruments
  5. FluorPen FP 110
  6. Instruction manual

PSI FluorPen FP 110 Instruction Manual

Hide thumbs Also See for FluorPen FP 110:
Table of Contents

Advertisement

Quick Links

Download this manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the FluorPen FP 110 and is the answer not in the manual?

Questions and answers

Related Manuals for PSI FluorPen FP 110

Summary of Contents for PSI FluorPen FP 110

  • Page 2 This document and its parts can be copied or provided to a third party only with the express permission of PSI. The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.

  • Page 3: Table Of Contents

    ABLE OF ONTENT Information before using FluorPen device ......................5 General Description ............................6 Technical Specification ..............................8 Device Description ............................11 List of equipment and customer information......................12 Care and maintenance ............................12 Principle of measurement ..........................13 Getting started ............................... 15 Pulses description and setting ..........................15 Measurement .................................18 Protocols explanation .............................18...
  • Page 4 Troubleshooting and Customer Support ......................50 Appendix ............................... 51 13.1 Battery pack for Monitoring Pen ..........................51 13.1.1 Standard battery pack ............................51 13.1.2 Extended temperature range battery pack ......................52 13.2 Installation and operation of the Monitoring Pen MP 100-A/B ................53 13.2.1 Device control .................................53 13.2.2 Device installation ..............................55...

  • Page 5: Information Before Using Fluorpen Device

    NFORMATION BEFORE USING LUOR EN DEVICE Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for clarification. By accepting the device, the customer agrees to follow the instructions in this guide. Always follow corresponding manuals while working with the FluorPen device or doing the maintenance.

  • Page 6: General Description

    FluorPen is also an invaluable tool for large plant-screening programs. PAR-FluorPen FP 110 includes all features of the FluorPen FP 110, and measures the same chlorophyll fluorescence parameters as FP110, but in addition, the PAR-FluorPen has an integrated Light Meter for direct digital readouts of Photosynthetically Active Radiation (PAR) in the range from 400 to 700 nm.
  • Page 7 Fig. 1 a) PAR-FluorPen FP 110/S. b) PAR-FluorPen FP 110/D. c) FluorPen FP 110/P. Monitoring Pen versions: Monitoring Pen MP 100-E (Fig. 2a) Monitoring Pen MP 100-E is a modified FluorPen designed for extra durability, battery-powered and intended for autonomous use in field conditions during extended experiments.

  • Page 8: Technical Specification

    2.1 T ECHNICAL PECIFICATION FluorPen and PAR-FluorPen Ft – instantaneous chlorophyll fluorescence Quantum Yield OJIP Protocols Non-photochemical quenching Light curve Photosynthetically Active Radiation (measured as PPFD) – PAR-FluorPen only LED emitter Blue (470 nm), other wavelengths on request Saturating pulse Illumination Up to 3,000 µmol(photon).m (adjustable from 10 to 100%) Actinic Illumination...
  • Page 9 Monitoring Pen Ft – instantaneous chlorophyll fluorescence Quantum Yield Protocols OJIP Non-photochemical quenching Light curve LED emitter Blue (470 nm), other wavelengths on request Saturating pulse Illumination Up to 3,000 µmol(photon).m (adjustable from 10 to 100%) Actinic Illumination Adjustable from 10 to 1,000 µmol(photon).m Measuring Illumination Up to 0,09 µmol(photon).m per pulse (adjustable from 10 to 100%)
  • Page 10 Bluetooth module compliance data: Category Country Standard FCC Part 15 Subpart B: 2008 Class B Radio FCC CRF Title 47 Part 15 Subpart C FCC ID: T9J-RN42 ETSI EN 301 489-1 V1.8.1 Europe ETSI EN 301 489-17 V2.1.1 ETSI EN 300 328 V1.7.1 Canada IC RSS-210 low power comm.

  • Page 11: Device Description

    EVICE ESCRIPTION Fig. 3 Device description. Page | 11...

  • Page 12: List Of Equipment And Customer Information

    • Battery charge lasts approximately 48 hours when the FluorPen is operated continuously. • If the battery can no longer be charged please contact PSI for replacement battery and installation instructions. Li-ion battery • Avoid fully discharging of the battery.

  • Page 13: Principle Of Measurement

    RINCIPLE OF MEASUREMENT FluorPen is a chlorophyll fluorometer and is used to measure different photosynthetic parameters in plants. It is equipped with a blue LED emitter (470 nm), optically filtered and precisely focused to deliver light intensities of up to 3,000 µmol.m-2.s-1 to measured plant tissue (Fig.
  • Page 14 Fig. 5 Chlorophyll fluorescence. FluorPen measures: Ft - Instantaneous Chlorophyll Fluorescence is equivalent to F if the sample is dark-adapted. QY - Quantum Yield QY is a measure of the Photosystem II efficiency. QY is equivalent to F in dark-adapted samples and to F in light-adapted samples.

  • Page 15: Getting Started

    ETTING STARTED For more detailed information on particular steps of FluorPen operation please refer to chapter 6. The device can is powered with built in Li-Ion battery. Ensure the battery is fully charged by plugging it into a PC via USB cable or the AC outlet via the USB cable and a USB adaptor (not included).
  • Page 16 Default setting of light color and intensities in FluorPen firmware. These may be changed according to user requirements and samples growth conditions: Flash pulse 30% = Measuring flash pulse Super pulse 80% = Saturating pulse Actinic pulse 300 µmol.m (30 %) = Actinic light Please note that those parameters are recommended by manufacturer and can be change according to user needs.
  • Page 17 Fig. 7 OJIP measurement performed with different intensities of Super pulse. Fig. 8 OJIP data - measurement performed with different intensities of Super pulse. The highest Fv/Fm value indicates the optimal intensity of Super pulse (20% in this case). Page | 17...

  • Page 18: Measurement

    Actinic pulse setting Intensity of Actinic pulse should correspond with cultivation light intensity or should be set according to application. Should Overflow be observed on the display during the measurement, lower the intensity of the used pulses. In case of Low value on display during measurement, increase the intensity of the used pulses. 5.2 M EASUREMENT No device calibration is needed before chlorophyll fluorescence measurements are made.
  • Page 19 The OJIP protocol includes the following measured and calculated parameters: Abbreviation Explanation Bckg Background , fluorescence intensity at 50 µs 50µs = fluorescence intensity at J-step (at 2 ms) = fluorescence intensity at i-step (at 30 ms) = maximal fluorescence intensity (maximal variable fluorescence) = ( F ) / ( F...

  • Page 20: Non-Photochemical Quenching (Npq) Protocols

    5.3.2 (NPQ) PHOTOCHEMICAL QUENCHING PROTOCOLS The NPQ protocol is used to quantify photochemical and non-photochemical quenching. It should be performed with dark-adapted samples. The NPQ protocol starts with measurement of minimal level of fluorescence F during a dark period. A short saturating flash of light is then applied to reduce the plastoquinone pool and measure maximum fluorescence in the dark-adapted state, F .
  • Page 21 _Ln is calculated as F / ((F – F ) / F _Ln). For more details, please refer to: Oxborough K., Baker N.R. (1997): Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components: calculation of qP and Fv’/Fm’ without measuring F0’. Photosynthesis Research 54: 135-142.
  • Page 22 Fig. 9 NPQ Protocol. Page | 22...

  • Page 23: Light Curve (Lc) Protocols

    5.3.3 (LC) IGHT CURVE PROTOCOLS The protocols called Light Curve (LC) were designed to acquire parameters for construction of Light Response Curve relating the rate of photosynthesis to photon flux density. The method is based on successive measurements of the sample exposed to a stepwise increase of light intensity.
  • Page 24 Fig. 10 LC1 Protocol. Page | 24...
  • Page 25 Fig. 11 LC2 Protocol. Page | 25...

  • Page 26: Multiple Measurement

    5.4 M ULTIPLE MEASUREMENT In addition to the single measurement of each available protocol, it is possible to perform also the multiple measurements of the same protocol over a period of time. The FluorPen may be set up to perform repeated measurements of the same parameter or protocol by selecting Settings >...

  • Page 27: Control Menu Tree

    ONTROL MENU TREE The next few pages of this manual show the structure of the menu and explain in a schematic way the operation of the FluorPen. The schematic diagrams show the Main Menu, first-level Sub-Menus and second-level Sub-Menus. • The blue color represents the Main Menu and its Options.
  • Page 28 Page | 28...
  • Page 29 Page | 29...
  • Page 30 Page | 30...
  • Page 31 Page | 31...
  • Page 32 Page | 32...
  • Page 33 Page | 33...
  • Page 34 Page | 34...

  • Page 35: Usb Connection

    PC. Both may be found on the installation disk (USB driver folder) delivered with the device. Once the USB driver is installed the Device Manager in Windows will list the USB serial port in the device tree. The USB driver may also be downloaded from PSI websites www.psi.cz .

  • Page 36: Bluetooth Connection

    LUETOOTH CONNECTION In addition to data transfer via USB the FluorPen may be connected to the software via Bluetooth for data transfer. Before setting up the Bluetooth connection between the FluorPen and the PC, ensure the following components are in place: Bluetooth enabled PC The PC must have Bluetooth wireless technology, either built-in or through a Bluetooth card.
  • Page 37 Select: “Add a device” to start searching for the new Bluetooth device. Be sure that the FluorPen is in discoverable mode (see step 1). Fig. 14 Adda device. Selecting the FluorPen • Select: PSI FluorPen icon. • Click: Next (Fig. 15). Fig. 15 Select the FluorPen. 5. Starting the Pairing Process This step is different for old and new version of the FluorPen, that are equipped with disparate Bluetooth module.
  • Page 38 Fig. 16 Pairing process. New version of FluorPen FP-110: • Select: Yes (Fig. 17). Please note that the FluorPen device does not display the verification number. The verification code is not important for the BT connection. • Select: Next. Fig. 17 Verifying of the Bluetooth pairing. Completing the FluorPen Pairing •...

  • Page 39: Fluorpen Software

    LUOR EN SOFTWARE 9.1 S OFTWARE INSTALLATION Copy the FluorPen software provided on the USB flash disk to your computer and launch the FluorPen program. To connect and recognize the FluorPen device in the FluorPen software, proceeds first with the registration of the FluorPen software (Fig. 19).

  • Page 40: Menu And Icons Explanation

    9.2 M ENU AND ICONS EXPLANATION 9.2.1 AIN MENU MENU: File Load Loads previously saved data files. Save Saves data to hard disc. Export Exports data in .txt format. Export to JSON Exports data in JavaScript Object Notation. Close Closes the current experiment. Close All Closes all running experiments.

  • Page 41: Menu Settings

    9.2.2 ENU SETTINGS MENU > Setup > Settings (Fig. After Download – Memory Erase If the box is checked the FluorPen memory is erased after each data download. Data – Inverted If the box is checked the polarity of data is inverted, e.g., multiplied by -1. This feature can be helpful for a certain type of experiment when the measured data are undesirably interpreted as negative values.
  • Page 42 Online Control – Values (Fig. The tab Values allows to set the measuring characteristics as light pulses intensities, measurement averaging and Multiple measurement. Please remember that each set value needs to be confirmed either by leaving the box or by pressing the ENTER key. The intensities of Actinic, Super and Flash Pulse light can be set in this window.
  • Page 43 Selection of the protocol for single measurements may be done under this tab in the software. Once the measurement is completed the data is saved to the device and can be downloaded to the PC at a later time. Measuring of PAR is active only in the PAR-FluorPen FP 110 (see picture below).

  • Page 44: Data Transfer And Visualization

    9.3 D ATA TRANSFER AND VISUALIZATION Once kinetic protocols data (OJIP, NPQ, LC) have been collected with the FluorPen to visualize the data it needs to be downloaded to the PC first via FluorPen software. Before data transfer can occur a successful connection between the FluorPen and the PC needs to be established via USB cable or Bluetooth module (see chapter 7 and 8 for details).
  • Page 45 Fig. 27 Graphic visualization of experiment. To export data from the FluorPen software select File > Export or Export icon. Select data type to export (Ft, QY, OJIP…) - Fig. 28. Selected only – exports only one measurement that is selected by mouse, otherwise it will export everything. Source data –...

  • Page 46: Firmware Update

    9.4 F IRMWARE UPDATE All data in the FluorPen memory are erased during the firmware update! Before starting any firmware update, download all your data from the FluorPen memory to the computer! Starting Update • Select: Setup > Update Firmware From File (Fig. 29). Fig.
  • Page 47 Fig. 31 Select .bxn file. Finishing Upload • Select: OK to start uploading of the update (Fig. 32). Fig. 32 Data loss warning. • The bottom bar indicates the upload progress (Fig. 33). Fig. 33 Upload progress. • Press: OK to finish upload (Fig. 34). Fig.

  • Page 48: Gps Module

    10 GPS MODULE The new versions of the FluorPen device (FP110) have integrated GPS module which can be turned on during the measurements. When GPS module is turned on the map coordinates will be automatically saved with all collected data and will be downloaded during data download.
  • Page 49 Fig. 36 GPS coordinates. Page | 49...

  • Page 50: Warranty Terms And Conditions

    The customer is responsible for shipping and insurance charges (for the full product value) to PSI. The manufacturer is responsible for shipping and insurance on return of the instrument to the customer. •...

  • Page 51: Appendix

    13 A PPENDIX 13.1 B ATTERY PACK FOR ONITORING Battery pack serves as an external power source for Monitoring Pen device. The external battery provides power during the long-term experiments. Please note that Monitoring Pen devices equipped with battery pack do not have internal battery, therefore it is not possible to use them without the battery pack.

  • Page 52: Extended Temperature Range Battery Pack

    Replacement of the battery: If the battery needs to be changed follow these steps: Unscrew 4 screws (in each corner of the battery casing) and remove the battery pack cover (Fig. 39 a). Disconnect the internal battery from the cables. Place new battery inside the casing, connect it with the cables –...

  • Page 53: Installation And Operation Of The Monitoring Pen Mp 100-A/B

    Connectors of Extended temperature range battery pack: Device - provides power connection between Monitoring Pen and the battery. This connection is necessary for Pen operation and data download. Serial - enables communication between Monitoring Pen and the PC for control and data transfer. Replacement of the battery: Follow these steps to replace the battery: Remove 4 screws in the corner of the case and remove the battery pack cover (Fig.
  • Page 54 Fig. 42 Online control window enables checking of the battery status. Fig. 43 Battery status. Page | 54...

  • Page 55: Device Installation

    The minimal battery voltage is 4.9 V. The device does not work at lower voltage. Please find more information about the battery pack in chapter 13.1. 13.2.2 D EVICE INSTALLATION Connect the Monitoring Pen to the cable. Place the Monitoring Pen under the water. Connect the device cable to the battery pack.

This manual is also suitable for:

Par-fluorpen fp 110Monitoring pen mp 100

Table of Contents