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(Please read this operation manual carefully to achieve the best and consistent column performance for a long time.)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the column package and the column surface for any damage.
(2) Please check the product name and serial number (Serial no. or S/N) written on the column package and
adhesive label on the column body.
(3) Please download the Certificate of Analysis (CoA) for the purchased product. The CoA can be downloaded
from Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number.
1. Introduction
Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based hydrophilic
chromatography (HILIC) chlromatography column modified with diol group. It is suitable for separation and
analysis of hydrophilic compounds such as oligosaccharides and oligonucleic acids.
2. Column Components
Please refer to the Shodex website: https://www.shodex.com/en/da/07.html
3. Column Specifications
Product
Product Name
Code
F6830100
HILICpak VN-50 10E
F6711400
HILICpak VN-50G 4A
Base Material
: Spherical porous particles of polyvinyl alcohol modified with diol group
Column Housing ：SUS-316 (VN-50 10E)
PEEK (VN-50G 4A)
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile = 25/75
4. Usable Conditions
Product Name
HILICpak VN-50 10E
HILICpak VN-50G 4A
Operation Manual
Shodex HILICpak VN-50 10E
Column Size (mm)
I.D.
10.0
4.6
Flow Rate (mL/min)
Recommended
Maximum
2 - 3
-
Resonac Corporation (https://www.shodex.com/)
Particle Size
(μm)
Length
250
5
10
5
Maximum
Pressure
(MPa/column)
5
15
-
-
ver.20221202E
Theoretical Plate Number
(Per Column)
≥ 11,000
(Guard Column)
pH
Temperature
Range
Range (°C)
2 - 13
4 - 60
1
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Related Manuals for Shodex HILICpak VN-50 10E
Summary of Contents for Shodex HILICpak VN-50 10E
- Page 1 Shodex website (https://www.shodex.com/download/). You will be asked to enter the serial number. 1. Introduction Thank you for purchasing the Shodex product. Shodex HILICpak VN-50 series is a polymer-based hydrophilic chromatography (HILIC) chlromatography column modified with diol group. It is suitable for separation and analysis of hydrophilic compounds such as oligosaccharides and oligonucleic acids.
- Page 2 (3) Suggested injection volume is less than 300 μL per column. (4) When a sample contains large amount of proteins or lipids, remove them prior to the sample injection.They may adsorb on the base material and damage the column. Resonac Corporation (https://www.shodex.com/)
- Page 3 Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. Resonac Corporation (https://www.shodex.com/)
- Page 4 Securely tighten the end caps and store the column at a location with stable temperature (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside the column to dry. It can damage the column. Resonac Corporation (https://www.shodex.com/)
- Page 5 9. Column Inspection Please refer to the inspection method described in the CoA. At Shodex, “half width method” is adopted for the calculation of plate count and “asymmetry factor (Fas)” is adopted for the calculation of peak symmetry. Please refer to the Shodex website for the detail: https://www.shodex.com/en/da/07.html...
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