Shodex RSpak DS-613 Operation Manual

Shodex RSpak DS-613 Operation Manual

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(Please read this manual carefully to achieve the best and consistent column performance for a long time)
Important Handling Instructions
Caution!
· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column
and understand their proper handling methods to prevent potential health hazards or death
from occurring.
· Please wear appropriate personal protective equipment such as lab goggles and gloves
when handling organic solvents and acid and alkaline reagents. Avoid any direct physical
contact to prevent chemical injuries.
Before Using the Column
(1) Please visually inspect the package and outside of the column for any damage.
(2) Please check if product name and serial number written on the column package, column name tag, and
enclosed Certificate of Analysis (CoA) are matching and correct.

1. Introduction

Thank you for purchasing the Shodex product. Shodex RSpak DS-613 is a polymer-based reversed phase
chromatography column. It can be used in a wide pH range from acidic to alkaline (pH 2 - 12).

2. Column Components

3. Column Specifications

Product
Product Name
Code
F7001001
RSpak DS-613
F6700140
RSpak DS-G
Base Material
: Spherical porous particles of styrene divinylbenzene copolymer
Column Housing : SUS-316
Screw Type
: Internally-threaded type No.10-32 UNF
Shipping Solvent : Water/Acetonitrile/THF = 30/40/30
Operation Manual
TM
Shodex
RSpak
Column Size (mm)
I.D.
6.0
4.6
SHOWA DENKO K.K. (https://www.shodex.com/)
TM
DS-613
Particle
Size (μm)
Length
150
6
10
10
ver.210702E
Theoretical Plate Number
(Per Column)
≥ 6,500
(Guard Column)
1

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Summary of Contents for Shodex RSpak DS-613

  • Page 1 Certificate of Analysis (CoA) are matching and correct. 1. Introduction Thank you for purchasing the Shodex product. Shodex RSpak DS-613 is a polymer-based reversed phase chromatography column. It can be used in a wide pH range from acidic to alkaline (pH 2 - 12).
  • Page 2 (3) Recommended injection volume is 20 μL per column. (4) For the samples containing proteins, remove the proteins prior to the sample injection. Note · Use of guard column is recommended to protect the analytical column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 3 This may result in creating an empty space in the column and deteriorates the column. Note · It is recommended to set the pump limiter to avoid exceeding the maximum pressure. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 4 (a cool and dark space is recommended). Refer to section 7.3 Solvent Exchange for how to replace the eluent. Attention! · Never allow inside of the column to dry. It can damage the column. SHOWA DENKO K.K. (https://www.shodex.com/)
  • Page 5 (2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications. For additional assistance, contact the distributor from whom you purchased the column or contact your regional Shodex...
  • Page 6 11. Warranty (Ver. 4COLUMN) (1) Showa Denko K. K. warrants that the Shodex product, at the time of delivery to the user, will conform to the specification of the attached CERTIFICATE OF ANALYSIS, if the Shodex product is used in accordance with the attached operating manual.

This manual is also suitable for:

Rspak ds-gF7001001F6700140

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