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Related Manuals for Analytik Jena Biometra TAdvanced Series
Summary of Contents for Analytik Jena Biometra TAdvanced Series
- Page 1 Operating Manual Biometra TAdvanced High-performance thermal cycler for DNA amplification by PCR...
- Page 2 Operating manual valid from software version ME 1.70 – RE 1.46 Follow these instructions for proper and safe use. Keep for future reference. 34-3800-000-23-e Documentation number B (11/2021) Edition Analytik Jena GmbH Implementation of the Technical Documentation © Copyright 2021, Analytik Jena GmbH...
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Page 3: Table Of Contents
Biometra TAdvanced Contents Contents Basic information ....................... 9 User manual notes ......................9 Intended use ........................10 Safety instructions ....................11 General notes ........................11 Safety markings on the Biometra TAdvanced ..............11 Technical condition ......................12 Requirements for the operating personnel ..............12 Safety instructions: transport and installation ............... - Page 4 Contents Biometra TAdvanced Block status ........................38 Quick start function ......................39 Incubation mode ......................42 Creating, editing and saving programs ..............47 Programming modes ....................... 47 Creating a new program/using a program template ............. 49 Assigning program names ....................51 Setting the heated lid temperature ................
- Page 5 Biometra TAdvanced Contents 11.3.3 Extended self-test log file ..................... 109 11.3.4 Error log file ........................110 11.4 Extended self-test ......................111 11.5 Service info file (SINF) ....................114 11.6 Backup ........................... 114 11.7 Cycler Information ......................115 11.8 Contact........................... 116 Adaptation of programs ..................
- Page 6 Figures Biometra TAdvanced Fig. 18 Incubation mode screen (Biometra TAdvanced Mono) ..........43 Fig. 19 Incubation mode screen (Biometra TAdvanced Twin) ........... 44 Fig. 20 Multiblock selection query (1) ..................45 Fig. 21 Multiblock selection query (2) ..................46 Fig. 22 Spreadsheet programming .....................
- Page 7 Biometra TAdvanced Figures Fig. 75 Automatic user logout screen ..................94 Fig. 76 Beeper configuration screen ................... 95 Fig. 77 Network settings screen, IP settings via static ............... 96 Fig. 78 Network settings screen, IP settings via DHCP .............. 96 Fig.
- Page 8 Figures Biometra TAdvanced...
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Page 9: Basic Information
Biometra TAdvanced Basic information Basic information User manual notes The Biometra TAdvanced is intended for operation by qualified specialist personnel ob- serving this operating manual. For the Biometra TAdvanced, there are 12 different models available. In the text below these devices are collectively called Biometra TAdvanced. Differences are explained in the corresponding sections. -
Page 10: Intended Use
Basic information Biometra TAdvanced Intended use The Biometra TAdvanced is an end-point thermal cycler. It is designed to amplify nucleic acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reac- tion. The thermal cycler is developed as product for general laboratory use. The Biometra TAdvanced combines modern design with user-friendly software. -
Page 11: Safety Instructions
Biometra TAdvanced Safety instructions Safety instructions General notes For your own safety and to ensure error-free and safe operation of the Biometra TAd- vanced, please read this section carefully before commissioning. Besides the safety instructions in this user manual and the local safety regulations that apply to the operation of the device, the general applicable regulations regarding acci- dent prevention, occupational health and safety and environmental protection have to be observed and complied with. -
Page 12: Technical Condition
Safety instructions Biometra TAdvanced Keep ventilation slits clear Keep the ventilation slits on the underside and at the rear of the device clear. Make sure that there are no objects underneath the device (e.g., paper) that may be sucked against the ventilation slot and thus inter- fere with ventilation. -
Page 13: Safety Instructions - Operation
Biometra TAdvanced Safety instructions Safety instructions – operation 2.6.1 General The operator of the Biometra TAdvanced must make sure before each commissioning that the condition of the device including the safety equipment is sound. The following has to be observed: ... -
Page 14: Handling Of Samples, Auxiliary And Operating Materials
Safety instructions Biometra TAdvanced The electrical components must be checked regularly by a qualified electrician. Any defects, such as loose connections, faulty or damaged cables, must be repaired with- out delay. Before opening the device it must be switched off at the power switch and the mains plug must be disconnected from the mains outlet! ... -
Page 15: Behavior During Emergencies
Biometra TAdvanced Safety instructions Behavior during emergencies In case of danger or accidents, immediately switch off the Biometra TAdvanced using the main switch on the equipment back plate. Disconnect the mains plug from the power supply! -
Page 16: Function And Setup
Function and setup Biometra TAdvanced Function and setup The Biometra TAdvanced thermal cycler is available in the following models: Model Biometra TAdvanced 96 with aluminum block Biometra TAdvanced 96 G with aluminum block with gradient function Biometra TAdvanced 96 S with silver block Biometra TAdvanced 96 SG with silver block... -
Page 17: High Speed Thermoblock
Biometra TAdvanced Function and setup Fig. 1 The Biometra TAdvanced Thermal Cycler The Biometra TAdvanced thermal cycler features an automatic restart. If a power failure occurs during the run, the device will continue the run as soon as power is restored. In case of long-term power failure (longer than 30 minutes), the device will keep the sam- ple block at 4 °C (freeze step) and the user can decide to repeat the run with the same samples or to discard them. -
Page 18: High Performance Smart Lid
Function and setup Biometra TAdvanced A rubber seal on the heated lid forms an encapsulated space around the sample block as soon as the lid is closed. The closed space serves to improve the temperature uniformity of the sample block and avoids the formation of condensed water at the final PCR cool- ing step. -
Page 19: Easy Programming
Biometra TAdvanced Function and setup comprehensive overview of the protocol structure without the need to access program- ming screens. The program preview is also available before the quick start of programs. Easy programming Creating new PCR programs takes a lot of time if for each single step all parameters have to be set manually. -
Page 20: Open System Philosophy
Function and setup Biometra TAdvanced device creates and stores log files for each single run. For long-term storage, the log files and extended self-test protocols can be exported as a proprietary file format. Open system philosophy The Biometra TAdvanced is an open system regarding consumables and reagents. De- pending on the sample block, the system can be used with single tubes, strips or plates. -
Page 21: Order Numbers
Biometra TAdvanced Function and setup Order numbers Instruments incl. block and base unit Model Block type Gradient range Block exchange Order numbers Biometra TAdvanced 96 Aluminum – 846-x-070-211 Biometra TAdvanced 96 G Aluminum 30 °C 846-x-070-201 Biometra TAdvanced 96 S Silver –... -
Page 22: Installation And Commissioning
Installation and commissioning Biometra TAdvanced Installation and commissioning Scope of delivery The scope of delivery includes: Thermal cycler Mains cable Operating instructions Quick reference guide Please keep the original packaging in case the device needs to be returned. The shipping box for the Biometra TAdvanced thermal cycler contains a specially developed foam sys- tem for the transport of high quality electronic devices. -
Page 23: Commissioning
Biometra TAdvanced Installation and commissioning Commissioning 4.4.1 General setup WARNING Danger of electric shock! Prior to commissioning, please make sure that the operating voltage set on the device is the same as the mains voltage (see section "Setting the oper- ating voltage"... - Page 24 Installation and commissioning Biometra TAdvanced For an alternative direct connection of the thermal cycler, the thermal cycler must be changed to a network connection with a fixed IP (e.g. IP 192.168.0.30, subnet mask 255.255.255.0) (please see chapter "Network“ 11.1.4). The network card must also be changed to a fixed IP(V4) via the network adapter settings under Windows (e.g.
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Page 25: Control Elements
Biometra TAdvanced Control elements Control elements Biometra TAdvanced front view Fig. 3 Front view of the Biometra TAdvanced Dial for adjusting the contact pressure Display Lid closing button USB port Biometra TAdvanced rear view The Ethernet port, mains plug and mains switch are located at the rear of the Biometra TAdvanced. -
Page 26: Quick Block Exchange
Control elements Biometra TAdvanced Quick block exchange The option for quick block exchange is available for all model types. To change the block: 1. Switch off the device. 2. Lift release lever on the backside (1 in Fig. 5) then pull block slowly to the back (2). 3. -
Page 27: Opening The Lid
Biometra TAdvanced Control elements NOTICE The strength of the lid contact pressure has been designed for a fully populated block. If only a few samples are to be used in the block, place an additional two (empty) tubes of the same height in the four corner positions of the block (see Fig. 6). Otherwise the sample tube may be damaged from excess contact pressure. -
Page 28: The Biometra Tadvanced Software
Control elements Biometra TAdvanced The Biometra TAdvanced software The Biometra TAdvanced thermal cycler features a 7" color touchscreen and a conven- ient software for operation. 5.5.1 Keyboard For some applications, names, addresses or passwords have to be defined. For this pur- pose, the Biometra TAdvanced software offers a keyboard to enter characters, digits and special characters. -
Page 29: Keypad
Biometra TAdvanced Control elements 5.5.2 Keypad In some screens of the Biometra TAdvanced software digits have to be entered. For this purpose, the software features a keypad integrated in some screens: Fig. 8 Keypad The keypad can be used to enter digits, dots, minus signs and infinite. In addition the following special keys are available: Delete single character Delete all characters... -
Page 30: List Of Commands And Icons Used
Control elements Biometra TAdvanced 5.5.5 List of commands and icons used The Biometra TAdvanced software uses the following icons: Command Icon Home Next Back Right Left Down Confirm Cancel Login User management User management ON/OFF New user Delete user Edit user Protocol Wizard New Polymerase Edit Polymerase... - Page 31 Biometra TAdvanced Control elements Command Icon Annealing temper- ature calculator Edit primer concen- tration Edit primer Calculate primer Save annealing temperature Ta Protocol Wizard Info Incubation Start incubation Programs New program/new from template Open template Copy program/copy all programs Delete program/ delete all programs Edit program Start program...
- Page 32 Control elements Biometra TAdvanced Command Icon Save program Save as Pause program Start program/ con- tinue program Skip step Choose block start program Block stop Graphical view Spreadsheet view Gradient display Edit step Insert step Delete step Temperature incre- ment Time increment Heating rate Gradient...
- Page 33 Biometra TAdvanced Control elements Command Icon Standard gradient programming Preheating OFF Preheating ON Tools Settings Date and time Automatic user log- Beeper Network Display brightness Display darker Display brighter Touchscreen cali- bration Factory settings Documentation Run log file Run log file over- view Save run log file to...
- Page 34 Control elements Biometra TAdvanced Command Icon Save all run log files to USB View run log file View program Power on log file Self-test log file Error log file Extended self-test Start extended self- test Stop extended self- test Service info file Backup Save backup file Load backup file...
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Page 35: Operation
Biometra TAdvanced Operation Operation Power on self-test After the Biometra TAdvanced is switched on, the device performs an automatic self- test (power on self-test). During the test the device shows the following screen: Fig. 9 Start screen For the power on self-test a log file is created and stored in the thermal cycler memory (see section "Power On Logfile"... -
Page 36: Login
Operation Biometra TAdvanced Biometra TAdvanced Mono Biometra TAdvanced Twin Fig. 10 Login screen Login After starting the Biometra TAdvanced, the login screen is displayed. To log in as an ex- isting user, press Login. Login In the next screen select a user by pressing the corresponding button. Fig. -
Page 37: Home Screen
Biometra TAdvanced Operation Touch the Password input field and enter the password using the Biometra TAdvanced keyboard (see section "Keyboard" p. 28). The default password for the Admin adminis- trator is "Admin". Please note that the password query is case sensitive. Fig. -
Page 38: Block Status
Operation Biometra TAdvanced Incubate For running a sample block at a constant See section "Incubation temperature mode" p. 42 Tools Settings, documentation, extended self- See section "Tools" p. 91 test, cycler information and diagnosis mode Logout User logout Block status In the login screen (see section "Login screen"... -
Page 39: Quick Start Function
Biometra TAdvanced Operation Running Yellow Program view Pause Blue Program view Quick start function Note: Programs can only be started by users with the right Start/stop programs acti- Biometra TAdvanced vated (see section "Editing user settings" p. 102). Mono Enable a user to start or stop programs The Biometra TAdvanced software offers a quick start function for previously used pro- grams. -
Page 40: Fig. 15 Program Quick Start Screen (Biometra Tadvanced Mono)
Operation Biometra TAdvanced Fig. 15 Program quick start screen (Biometra TAdvanced Mono) Select the program for quick start in the table and press the Start button to start the se- lected program. If necessary, the program can be edited before the start or a new pro- gram can be created based on a template (see section "Programming modes"... -
Page 41: Fig. 16 Program Quick Start Screen (Biometra Tadvanced Twin)
Biometra TAdvanced Operation screen (see section "Login screen" p. 35) or in the home screen (see section "Home screen" p. 37). Note: The quick start function can only be activated if the current block status is Free (see section "Quick start function" p. 39). If the status is Running or Pause, a touch of the button will lead to the program view screen instead. -
Page 42: Incubation Mode
Operation Biometra TAdvanced To start a program on both sample blocks press the following button: Choose block start program By activating the checkboxes select the sample blocks for program start. Fig. 17 Multiblock selection query Note: Programs offered for quick start can only be edited or deleted by users with the right Write/Delete own programs activated (see section "Editing user settings"... -
Page 43: Fig. 18 Incubation Mode Screen (Biometra Tadvanced Mono)
Biometra TAdvanced Operation Incubate Note: To set the time to endless, use the button: Endless time To start incubation, press the Start button Start Fig. 18 Incubation mode screen (Biometra TAdvanced Mono) During incubation the following commands can be used: Stop Pause Continue... -
Page 44: Fig. 19 Incubation Mode Screen (Biometra Tadvanced Twin)
Operation Biometra TAdvanced Note: Programs can only be started by users with the right Start/stop programs acti- Biometra TAdvanced Twin vated (see section "Editing user settings" p. 102). Enable a user to start or stop programs The incubation mode allows samples to be incubated at a constant temperature for an indefinite period of time. -
Page 45: Fig. 20 Multiblock Selection Query (1)
Biometra TAdvanced Operation Sample block 1 Sample block 2 To start incubation on both sample blocks press the following button: Choose block start program By activating the checkboxes select the sample blocks for program start. Fig. 20 Multiblock selection query (1) During incubation the following commands can be used: Stop Block Stop... -
Page 46: Fig. 21 Multiblock Selection Query (2)
Operation Biometra TAdvanced Fig. 21 Multiblock selection query (2) -
Page 47: Creating, Editing And Saving Programs
Biometra TAdvanced Creating, editing and saving programs Creating, editing and saving programs Programming modes The Biometra TAdvanced software offers spreadsheet or graphical programming. Use the buttons to toggle between the two modes. The following screenshots show the spreadsheet and graphical programming mode. Graphical view Spreadsheet view Fig. -
Page 48: Fig. 23 Graphical Programming
Creating, editing and saving programs Biometra TAdvanced Fig. 23 Graphical programming Both programming modes show the same information but in different ways. In the spreadsheet mode all parameters are summarized in a table. In the graphical mode sym- bols are displayed for some parameters (see the table below). Toggle to the spreadsheet mode to view the settings for these parameters. -
Page 49: Creating A New Program/Using A Program Template
Biometra TAdvanced Creating, editing and saving programs If a program has more than 6 steps, use the cursor keys to navigate in the spreadsheet or the graphical display. Creating a new program/using a program template Note: Only when the right Write/delete own programs is activated, ... -
Page 50: Fig. 25 Blank Program Template
Creating, editing and saving programs Biometra TAdvanced 4. The program template screen opens (see Fig. 26). Search for a program template us- ing the cursor keys and press the corresponding button to select it. Down Note: The Biometra TAdvanced offers several program templates for different purposes. You can either use a template and modify it as desired or select the option Blank. -
Page 51: Assigning Program Names
Biometra TAdvanced Creating, editing and saving programs Fig. 26 Program template screen Assigning program names Each template has a specific name which is superimposed in the programming screen. To enter a name, touch the Name input field and enter a new name using the Biometra TAdvanced keyboard. -
Page 52: Preheating The Heated Lid
Creating, editing and saving programs Biometra TAdvanced Fig. 27 Heated lid temperature setting screen NOTICE The heated lid automatically switches off at a temperature difference of >75 °C between the block and the heated lid. At these low block temperatures, sample condensation on the tube lid is no longer to be expected. -
Page 53: Editing All Parameters Of A Step
Biometra TAdvanced Creating, editing and saving programs Parameter Description and limits Temperature Temperature for a step in °C. The target temperature can be set between 3.0°C and 99.0°C in tenths of a degree. Hold time Hold time for a step in h:mm:ss. Values between 0 and 9 hours, 0 and 59 minutes and 0 to 59 seconds can be programmed. -
Page 54: Programming Loops
Creating, editing and saving programs Biometra TAdvanced Fig. 28 Program step editing screen Note: To program a step at least the temperature and the hold time must be defined. 3. To set the temperature, touch the corresponding input field and enter a value be- tween 3.0°C and 99.0°C. -
Page 55: Programming A Temperature Increment
Biometra TAdvanced Creating, editing and saving programs 2. To define the number of repetitions, enter a number in the Cycles field (see Fig. 28). For example, to program the loop for the following protocol, select step 4 and enter the value 2 in the Go To input field and the value 35 in the Cycles input field. -
Page 56: Adjusting The Ramp Rate
Creating, editing and saving programs Biometra TAdvanced Time increment (∆t) Note: Time increments only become active if they are in loops. If a time increment has been programmed, the small symbol will be shown at the corresponding step in the graphical programming mode. -
Page 57: Fig. 30 Temperature Gradient On A 96 Well Sample Block
Biometra TAdvanced Creating, editing and saving programs Fig. 30 Temperature gradient on a 96 well sample block In a specific column all wells have the same temperature but the temperature differs from column to column. The temperature gradient can be applied in both directions; the highest temperature can be either in column 1 or column 12. -
Page 58: Fig. 31 Linear Gradient Tool Screen (Biometra Tadvanced Mono)
Creating, editing and saving programs Biometra TAdvanced Fig. 31 Linear Gradient Tool screen (Biometra TAdvanced Mono) To create a gradient using the standard gradient programming screen, enter a tempera- ture for the first column and the last column in the sample block (see Fig. 32). The ef- fective temperatures for all the other columns are automatically calculated and displayed as a bar chart. -
Page 59: Fig. 33 Temperature Gradient On A 48 Well Sample Block
Biometra TAdvanced Creating, editing and saving programs Fig. 33 Temperature gradient on a 48 well sample block In a specific line all wells have the same temperature but the temperature differs from line to line. The temperature gradient can be applied in both directions; the highest temperature can be either in line 1 or line 8. -
Page 60: Fig. 34 Linear Gradient Tool Screen (Biometra Tadvanced Twin)
Creating, editing and saving programs Biometra TAdvanced Fig. 34 Linear Gradient Tool screen (Biometra TAdvanced Twin) To create a gradient using the standard gradient programming screen, enter a tempera- ture for the first line and the last line in the sample block (see Fig. 35). The effective temperatures for all the other lines are automatically calculated and displayed as a bar chart. -
Page 61: Direct Programming Of Program Parameters For A Step
Biometra TAdvanced Creating, editing and saving programs Fig. 36 Temperature gradient query 7.6.2 Direct programming of program parameters for a step The Biometra TAdvanced software allows individual parameters (temperature, hold time, loops, temperature and time increment, as well as the ramp rate) for a step to be edited directly in the graphical or spreadsheet view. -
Page 62: Fig. 38 Graphical Program View
Creating, editing and saving programs Biometra TAdvanced ⑦ Ramp rate ∆R (°C/s) To edit program parameters in the graphical view, press the corresponding input field for the parameter that you want to edit for the step (see Fig. 38). Program options (loops, temperature and time increment, as well as the ramp rate) must be activated using a button to the left of the graphical view (see Fig. -
Page 63: Fig. 39 Individual Program Parameter Editing
Biometra TAdvanced Creating, editing and saving programs Fig. 39 Individual program parameter editing If the sample block is gradient-enabled, you also have the option of programming a gra- dient step. To do this, when editing the temperature, press the Grad button. Gradient A gradient step can be programmed here (see Fig. -
Page 64: Inserting A Step
Creating, editing and saving programs Biometra TAdvanced Inserting a step To insert a step, select a program step on the Biometra TAdvanced spreadsheet or graphical programming screen and press the Insert button. Insert When you press the Insert button, the screen for editing program steps opens (see sec- tion "Editing steps"... -
Page 65: Fig. 41 Program Overview Screen
Biometra TAdvanced Creating, editing and saving programs Note: If a new program is to be saved, the first free storage location in the directory of the currently logged in user is automatically selected. However, the storage location can be changed using the cursor keys above and below the lists. If the selected storage loca- tion is already in use, the stored program will not be overwritten;... -
Page 66: Fig. 42 Quick Selection Screen
Creating, editing and saving programs Biometra TAdvanced Fig. 42 Quick selection screen Before saving the program there is still the possibility of changing the program name. To do this, press the Save as button and enter the new program name using the Bi- ometra TAdvanced keyboard (see section "Assigning program names"... -
Page 67: Starting, Coping And Deleting Programs
Biometra TAdvanced Starting, coping and deleting programs Starting, coping and deleting programs Starting a program Note: Programs can only be started by users with the right Start/stop programs acti- vated (see section "Editing user settings" p. 102). Additionally the right Read all pro- grams defines, if a user is allowed to read all programs or if the access is limited to own programs. -
Page 68: Fig. 44 Program Overview Screen With Preview
Starting, coping and deleting programs Biometra TAdvanced Touch the corresponding buttons to select a user directory and a stored program. If the number of user directories or programs is higher than the maximum number that can be displayed in the list, the buttons above and below the lists can be used to scroll up and down. -
Page 69: Copying A Program
Biometra TAdvanced Starting, coping and deleting programs Copying a program Note: Users can only copy and save their own programs when the right Write/delete own programs is activated. Programs of others can be copied and saved with the right Write/delete all programs activated (see section "Editing user settings" p. 102). Enable a user to copy and save own programs Enable a user to copy and save all pro-... -
Page 70: Copying All Programs
Starting, coping and deleting programs Biometra TAdvanced Fig. 46 Program overview screen Copying all programs Note: Users can only copy and save their own programs when the right Write/delete own programs is activated. Programs of others can be copied and saved with the right Write/delete all programs activated (see section "Editing user settings"... -
Page 71: Deleting A Program
Biometra TAdvanced Starting, coping and deleting programs Note: The programs will be copied into the selected user directory beginning at the first free storage location. Fig. 47 Program overview screen Deleting a program Note: Users can only delete their own programs when the right Write/delete own pro- grams is activated. -
Page 72: Deleting All Programs
Starting, coping and deleting programs Biometra TAdvanced Fig. 48 Confirmation prompt Note: If a program is deleted, all the following programs will be moved one storage loca- tion lower. The storage location number for these programs will decrease by -1. Deleting all programs Note: Users can only delete their own programs when the right Write/delete own pro- grams is activated. -
Page 73: Fig. 49 Confirmation Prompt
Biometra TAdvanced Starting, coping and deleting programs Fig. 49 Confirmation prompt... -
Page 74: Running, Pausing, Continuing And Stopping Programs
Running, pausing, continuing and stopping programs Biometra TAdvanced Running, pausing, continuing and stopping programs To switch the display of the sample blocks during a run press the button with the num- Biometra TAdvanced Twin ber of the corresponding sample block. Sample block 1 Sample block 2 Display during a run... -
Page 75: Fig. 51 Spreadsheet View
Biometra TAdvanced Running, pausing, continuing and stopping programs Fig. 51 Spreadsheet view Fig. 52 Display during the run in a graphical, spreadsheet and gradient view... -
Page 76: Fig. 53 Graphical View
Running, pausing, continuing and stopping programs Biometra TAdvanced Biometra TAdvanced Twin Fig. 53 Graphical view Fig. 54 Spreadsheet view... -
Page 77: Pausing A Program
Biometra TAdvanced Running, pausing, continuing and stopping programs Fig. 55 Display during the run in a graphical, spreadsheet and gradient view The following parameters are summarized in the headline in the table: Program name Sample block temperature Estimated end ... -
Page 78: Fig. 56 Spreadsheet View
Running, pausing, continuing and stopping programs Biometra TAdvanced Pause The word "Pause" is shown and the pause time is counted in the Remain. Time field. Biometra TAdvanced Mono Fig. 56 Spreadsheet view Fig. 57 Display screen during a program pause (graphical view) -
Page 79: Fig. 58 Spreadsheet View
Biometra TAdvanced Running, pausing, continuing and stopping programs Biometra TAdvanced Twin Fig. 58 Spreadsheet view Fig. 59 Display screen during a program pause (graphical view) Note: Pressing the Pause button requires manual interaction. The Biometra TAdvanced software also allows pauses to be programmed (see section "Editing steps" p. 52), then the pause step is automatically started by the device. -
Page 80: Continuing The Program
Running, pausing, continuing and stopping programs Biometra TAdvanced Continuing the program To continue a paused program, press the Continue button on the Biometra TAdvanced run display screen (see Fig. 57 p. 78). Continue The Biometra TAdvanced will continue the program from exactly the point at which it was paused. -
Page 81: Fig. 60 Confirmation Prompt
Biometra TAdvanced Running, pausing, continuing and stopping programs Fig. 60 Confirmation prompt After confirmation of the confirmation prompt the Biometra TAdvanced stops the cur- rent program. If two sample blocks are running the Biometra TAdvanced offers the option to stop only Biometra TAdvanced Twin one block or to stop both blocks simultaneously. -
Page 82: Protocol Wizard
Program templates for up to 8 polymerases can be stored by the TAdvanced soft- ware. Fig. 63 Select polymerase screen Pre-installed in the software is the program template for Analytik Jena Innupure poly- merases. To create a PCR program select a polymerase from the list and press the Next button. Next In the next screen (see section "Enter a new polymerase or edit a polymerase"... -
Page 83: Enter A New Polymerase Or Edit A Polymerase
Biometra TAdvanced Protocol Wizard 10.2 Enter a new polymerase or edit a polymerase To enter program templates for new polymerases or to edit preset program templates for polymerases press the corresponding button: New polymerase Edit polymerase In the next screen (see Fig. 64) touch the corresponding input boxes to enter a name for the polymerase and set parameters for all requested program steps. -
Page 84: Delete A Polymerase
Protocol Wizard Biometra TAdvanced number of polymerases than can be displayed in the list, the cursor key function above and below the polymerase list can be used to scroll up and down. Fig. 65 Select polymerase screen Note: If a new or edited polymerase is to be saved, the first free storage place in the pol- ymerase list is pre-selected. -
Page 85: Protocol Wizard Parameter Screen
Biometra TAdvanced Protocol Wizard Delete Polymerase Note: The Template InnuTaq cannot be deleted. 10.4 Protocol Wizard parameter screen For automatic calculation of PCR programs in the protocol wizard parameter screen (see Fig. 67) some parameters have to be set by the user: 1. -
Page 86: Fig. 67 Protocol Wizard Parameter Screen
Protocol Wizard Biometra TAdvanced Fig. 67 Protocol wizard parameter screen 7. After setting all program parameters press the Next button. Next The next screen lists the all steps of the program that will be created by the software: Fig. 68 Protocol wizard view parameter screen Press the create program button to enter the TAdvanced programming screen (see sec- tion "Creating, editing and saving programs"... -
Page 87: Primer Annealing Temperature Calculator
Biometra TAdvanced Protocol Wizard Create program Note: Programs have to be saved before they can be started. If the program has been created but should not be saved confirm the following security query to discard the pro- gram: Fig. 69 Security query 10.5 Primer Annealing Temperature Calculator Based on the primer sequences the Biometra TAdvanced protocol wizard can calculate... -
Page 88: Fig. 70 Primer Annealing Temperature Calculator Screen
Protocol Wizard Biometra TAdvanced Fig. 70 Primer annealing temperature calculator screen To adjust the primer or salt concentration press the Edit concentration button. Edit concentration In the next screen the primer concentration, salt concentration and magnesium concen- tration can be adjusted. Touch the corresponding input field and enter values using the keypad. -
Page 89: Fig. 71 Edit Primer Concentration Screen
Biometra TAdvanced Protocol Wizard Fig. 71 Edit primer concentration screen Press the Edit primer button to toggle to the primer annealing temperature calculator screen (see Fig. 70) or press the Calc. primer button to calculate the theoretical primer annealing temperature (T Edit Primer Calc. - Page 90 Protocol Wizard Biometra TAdvanced Where w, x, y and z are the number of the bases A, T, G and C in the primer se- quence, respectively. 2. The average T value is calculated from the T values for both primer sequences. 3.
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Page 91: Tools
Biometra TAdvanced Tools Tools The Biometra TAdvanced software provides the following tools: Settings Defines basic device settings See section "Configuration" p. 91 User Management Tool for user management See section "User management" p. 99 Documentation Access to stored log files See section "Documentation"... -
Page 92: Setting The Date And Time
Tools Biometra TAdvanced The Biometra TAdvanced software offers the following setting options: Date and time Sets the date and time See section "Setting the date and time" p. 92 Automatic user logout Switches automatic user logout on or off and See section "Automatic user logout"... -
Page 93: Automatic User Logout
Biometra TAdvanced Tools Fig. 74 Date and time entry screen Activate the corresponding input field and use the keypad on the right side of the date and time entry screen to enter digits. The following buttons may also be helpful when completing the fields: Delete single character Delete all characters... -
Page 94: Configuring The Beeper
Tools Biometra TAdvanced Automatic logout On Automatic logout Off If automatic user logout is switched on, the time after which the user is logged out from the system can be set. You can enter values between 30 and 60 minutes. Fig. -
Page 95: Network
Biometra TAdvanced Tools Fig. 76 Beeper configuration screen 11.1.4 Network The Network menu item can be used to configure basic settings or show a list of net- work users. Press the corresponding button Network settings Network user Note: The network settings can only be configured by users with the right System con- Network settings figuration activated (see section "Editing user settings"... -
Page 96: Fig. 77 Network Settings Screen, Ip Settings Via Static
Tools Biometra TAdvanced Go to the next input field Fig. 77 Network settings screen, IP settings via static Fig. 78 Network settings screen, IP settings via DHCP Note: The network cable must be at least performance class Cat 5e and the cable config- uration has to be STP. -
Page 97: Display Brightness
Biometra TAdvanced Tools Live monitoring of PCR runs on network-integrated thermal cyclers- Starting and stopping PCR protocols are stored on the thermal cycler Notification system for monitoring status changes on selected blocks of a thermal cy- cler Store PCR protocols on the terminal. This can be stored on a PC using a USB stick ... -
Page 98: Screen Calibration
Tools Biometra TAdvanced 11.1.6 Screen calibration Note: The touch screen can only be calibrated by users with the right System configura- tion activated (see section "Editing user settings" p. 102). Enable a user to configure the system configuration To calibrate the screen, follow the instructions and touch the screen at the center of the displayed circle. -
Page 99: User Management
Biometra TAdvanced Tools Fig. 81 Confirmation prompt Using the factory settings tool will delete all users and programs! Use a backup file to re- store the system (see section "Backup" p. 114). 11.2 User management The Biometra TAdvanced can manage up to 90 user directories. User management al- lows new users to be created and user rights to be set. -
Page 100: Fig. 82 User Management Screen
Tools Biometra TAdvanced Fig. 82 User management screen In the next screen activate the corresponding checkbox to configure the Biometra TAd- vanced user management: Fig. 83 Configure user management screen If the user management is switched off, all new users have all rights and can access all software functions. -
Page 101: Creating A User
Biometra TAdvanced Tools Write/delete all pro- grams Write/delete own pro- grams Read all programs Start/stop programs Show your own pro- grams to other users * An administrator can create users of all groups and modify their rights settings. A user with generic rights is only allowed to create users with generic or limited rights but no administrator. -
Page 102: Editing User Settings
Tools Biometra TAdvanced New user In the next screen enter the using the Biometra TAdvanced keyboard (see section "Key- board" p. 28) Username (up to 13 characters or numbers) Initials (2 to 3 characters) Password (up to 13 characters, numbers or special characters) ... -
Page 103: Fig. 85 User Data Screen
Biometra TAdvanced Tools Edit User Account On the next screen the user data and the language settings can be changed. Fig. 85 User data screen Note: For the administrator Admin the user name and the initials ADM cannot be modi- fied. -
Page 104: Fig. 86 User Properties Screen
Tools Biometra TAdvanced Fig. 86 User properties screen Right Function System configuration Provides access to several system settings like date and time, screen saver, beeper, network settings, display brightness, touch screen calibration and factory settings Edit user Allows to modify the user name, user initials, password, lan- guage setting and user rights Create user Allows to create new user accounts. -
Page 105: Delete User
Biometra TAdvanced Tools 11.2.3 Delete user Note: User accounts can only be deleted by users with the right Delete User activated. Enable a user to delete users To delete a user, select the user on the user management screen (see section "User man- agement"... -
Page 106: Run Log File
Tools Biometra TAdvanced Fig. 88 Documentation screen Press the corresponding button to access the desired function. 11.3.1 Run log file The Biometra TAdvanced lists up to 24 run log files for the programs that were last started. Fig. 89 Run log file overview screen The run log files are sorted by date and time. -
Page 107: Fig. 90 Run Log File Screen
Biometra TAdvanced Tools If more than 6 run log files have been created, you can use the following cursor keys to navigate through the table with the run log files (see Fig. 89). Down To view the collected data, select a run log file by activating the corresponding row in the table and press the View Run Log File button. -
Page 108: Fig. 91 Program View Screen
Tools Biometra TAdvanced The Biometra TAdvanced offers the option to show the program in graphical from or as a spreadsheet (see section "Programming modes" p. 47). To toggle between the graph- ical and spreadsheet view, use the buttons: Graphical view Spreadsheet view Fig. -
Page 109: Power On Logfile
Biometra TAdvanced Tools 11.3.2 Power On Logfile With each system start, the Biometra TAdvanced performs an initial self-test and stores the results in a power on logfile. In the power on logfile screen the following information is listed: Power-on by date and time ... -
Page 110: Error Log File
Tools Biometra TAdvanced Fig. 93 Extended self-test log file screen The test result can be Passed or Failed and is listed in the last row of the table for each test. 11.3.4 Error log file The Biometra TAdvanced records all errors by date and time. The latest error is shown at the first position in the error table. -
Page 111: Extended Self-Test
Biometra TAdvanced Tools 11.4 Extended self-test NOTICE The Extended Self-Test must be performed under the following conditions to achieve re- producible and meaningful results: Ambient temperature in the range 20 °C to 25 °C Mains voltage depending on the voltage type of the device: 100 V/115 V/230 V, ±10 % each ... -
Page 112: Fig. 95 Sample Block Loaded With Plasticware For The Extended Self-Test
Tools Biometra TAdvanced 96 well sample block (Biometra TAdvanced Mono) 48 well sample block (Bioemtra TAdvanced Twin) Fig. 95 Sample block loaded with plasticware for the extended self-test When the plasticware has been properly inserted and the heated lid is closed, the ex- tended self-test can be started. -
Page 113: Fig. 97 Extended Self-Test Overview Screen (Biometra Tadvanced Mono)
Biometra TAdvanced Tools Cancel Self Test If the extended self-test is canceled, either no log file or an incomplete log file will be created. Fig. 97 Extended self-test overview screen (Biometra TAdvanced Mono) Fig. 98 Extended self-test overview screen (Biometra TAdvanced Twin) The results of the extended self-test are summarized in a log file, stored by the device and can be viewed by the user (see section "Extended self-test log file"... -
Page 114: Service Info File (Sinf)
Tools Biometra TAdvanced 11.5 Service info file (SINF) The service info file is a helpful tool for remote fault diagnosis by the Biometra service department. Fig. 99 Service info file creation screen To create a service info file, connect a USB stick to the Biometra TAdvanced and press the Info File to USB button. -
Page 115: Cycler Information
Biometra TAdvanced Tools Fig. 100 Backup file screen Connect a USB stick to the device and press the Save backup file button to create a backup file. Save backup file To load a backup file, select it in the list and press the Load backup file button. Load backup file Backup files that are no longer used can be deleted using the functions: Delete all backup files... -
Page 116: Contact
Tools Biometra TAdvanced Protocol version Software version IO The information is summarized in two screens. Press the System/Cycler Info button to toggle between the two screens. System/Cycler Info Fig. 101 Cycler Info screen 11.8 Contact Shows contact details for the Biometra service department. Fig. -
Page 117: Adaptation Of Programs
Biometra TAdvanced Adaptation of programs Adaptation of programs If programs are exchanged between different thermal cycler models or the sample block is exchanged, programs might need to be adapted to the installed sample block type prior to the start or during editing. The following table provides an overview of possible program adaptations: Cause Example... -
Page 118: Quick Reference Guide
Quick reference guide Biometra TAdvanced Quick reference guide WARNING Before initial operation check the correct operating voltage is set on the voltage selec- tion switch on the underside of the device. Do not open the housing of the device unless you are authorized to do so. - Page 119 Biometra TAdvanced Quick reference guide Press Logout to log out. Home screen for Biometra TAdvanced Mono for Biometra TAdvanced Twin Press Programs on the home screen to open the program overview: Editing, saving and copy- ing programs To edit a template, press New from template, select a template from the list and press Open template.
- Page 120 Quick reference guide Biometra TAdvanced To switch the heated lid preheating on or off, press the corresponding button. Select a program step and press Edit. The screen for editing program steps opens: Use the cursor keys above the keyboard to switch between program steps. The num- ber of the currently activated step is displayed on a blue background in the table at the top of the screen.
- Page 121 Biometra TAdvanced Quick reference guide the graphical view press on the desired parameter edit field. Program options (loops, temperature and time increment, heating and cooling rate) must be activated using the button to the left of the graphical display. To insert a program step, select a step and press Insert. In the next screen enter the desired parameters and confirm the settings by pressing .
- Page 122 Quick reference guide Biometra TAdvanced Spreadsheet view Graphical view...
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Page 123: Fault Removal
Biometra TAdvanced Fault removal Fault removal 14.1 Administrator password forgotten If the administrator has forgotten his password, use the Factory settings function (see section "Factory settings" p. 98) to reset the system and download a backup file for re- covery (see section "Backup" p. 114). 14.2 Slow heating and cooling The Biometra TAdvanced is equipped with strong fans that remove the heat from the heat sink. -
Page 124: Adaptation Of Programs From Other Thermal Cyclers
Fault removal Biometra TAdvanced 14.5 Adaptation of programs from other thermal cyclers Since the Biometra TAdvanced thermal cycler is a very fast device, it may be necessary to reduce the heating and cooling ramp when running protocols from other (slower) ther- mal cyclers (see section "Adjusting the ramp rate"... - Page 125 Biometra TAdvanced Fault removal Note: This applies to usual PCR conditions with up to 95 °C for denaturation and with a room air humidity up to 70 %. In case you are using higher denaturation temperatures than 95 °C or if the local room air humidity is higher than 70 %, then you should set at least the same lid temperature you are using as highest block temperature.
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Page 126: Maintenance And Care
Maintenance and care Biometra TAdvanced Maintenance and care The Biometra TAdvanced is mainly maintenance-free. The care and maintenance tasks which can be performed by the customer are limited to the cleaning and disinfection of the housing and sample block. All maintenance work and repairs beyond this scope must only be performed by Bi- ometra GmbH customer service personnel or authorized and trained persons. -
Page 127: Disinfecting The Device
Biometra TAdvanced Maintenance and care 15.2 Disinfecting the device WARNING Biological hazard! Clean the Biometra TAdvanced with particular care after analyzing potentially infectious material. Wear suitable protective equipment, e.g., protective gloves. NOTICE The only suitable cleaning method for the housing is wipe disinfection. When using spray disinfectants there is a risk that the liquid enters the sensitive elec- tronic system through the ventilation slits. -
Page 128: Transport
16.1 Device return information WARNING Risk of damage to health due to improper decontamination! Perform a professional de- contamination before returning the device to Analytik Jena AG. NOTICE Biometra GmbH must refuse acceptance of contaminated devices. The sender may be liable for any damage caused by inadequate decontamination of the device. -
Page 129: Packing The Biometra Tadvanced
Biometra TAdvanced Transport 16.2 Packing the Biometra TAdvanced Biometra uses an extra designed packaging system with inserts of foamed material. NOTICE The thermal cycler is only protected from transport damage if the original packaging is used and the packing instructions are followed. Biometra is not liable for transport dam- age due to incorrect packaging. -
Page 130: Disposal
Disposal Biometra TAdvanced Disposal The user of the Biometra TAdvanced must dispose of any waste materials that occur during measurements (sample materials) in accordance with statutory and local regula- tions. At the end of its service life, the Biometra TAdvanced and all its electronic components must be disposed of as electronic waste in accordance with valid regulations. -
Page 131: Specifications
Biometra TAdvanced Specifications Specifications 18.1 Model specific data There is a range of 12 models with monoblocks or twinblocks, with specific capacities and block materials as well as with and without gradient function. Order number 846-x-070-211 846-x-070-201 Model Biometra TAdvanced 96 Biometra TAdvanced 96 G Capacity 96 x 0.2 ml tubes, 96 well micro plates or 12 x 8 well strips... - Page 132 Specifications Biometra TAdvanced Order number 846-x-070-251 846-x-070-241 Model Biometra TAdvanced 96 S Biometra TAdvanced 96 SG Capacity 96 x 0.2 ml tubes, 96 well micro plates or 12 x 8 well strips Block material Silver Block coating Gold Block exchange (optional) Quick-X-change Block exchange time less than 10 sec...
- Page 133 Biometra TAdvanced Specifications Order number 846-x-070-210 846-x-070-200 Model Biometra TAdvanced 60 Biometra TAdvanced 60 G Capacity 60 x 0.5 ml tubes Block material Aluminum Block coating Special alloy (gold color.) Block exchange (optional) Quick-X-change Block exchange time less than 10 sec Max.
- Page 134 Specifications Biometra TAdvanced Order number 846-x-070-224 846-x-070-214 Model Biometra TAdvanced 384 Biometra TAdvanced 384 G Capacity 384 well micro plates Block material Aluminum Block coating Special alloy Block exchange (optional) Quick-X-change Block exchange time less than 10 sec Max. heating rate* 4.0 °C/sec Max.
- Page 135 Biometra TAdvanced Specifications Order number 846-x-070-212 846-x-070-202 Model Biometra TAdvanced Twin 48 Biometra TAdvanced Twin 48 G Capacity Two blocks with 2 x 48 x 0.2 ml tubes, 2 x 48 well micro plates or 2 x 6 x 8 well strips Block material Aluminum Block coating...
- Page 136 Specifications Biometra TAdvanced Order number 846-x-070-213 846-x-070-215 Model Biometra TAdvanced Twin 30 Biometra TAdvanced Twin combi Capacity Two blocks with Two blocks with 2 x 30 x 0.5 ml tubes 2 x 48 x 0.2 ml tubes, 2 x 48 well micro plates 2 x 6 x 8 well strips or 2 x 35 x 0.5 ml tubes** Block material...
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Page 137: Specifications For All Models
Biometra TAdvanced Specifications 18.2 Specifications for all models The following specifications apply to all models: Software User-specific quick start option for the five most recent programs; pro- gram preview before start; option for toggling between spreadsheet and graphical programming mode; Linear Gradient Tool , service info file (SINF) generation;... -
Page 138: Declaration Of Conformity
Declaration of conformity Biometra TAdvanced Declaration of conformity... -
Page 139: Index
Biometra TAdvanced Index Index Auto restart 118 Administrator 35 Backup 110 Forgotten 118 Block module exchange 24 User 98 Block status 37 Personnel 12 Care 121 Power on self-test 34 Cleaning the housing 121 Program Configuration 87 Continue 77 Declaration of conformity 133 Copy 67 Decontamination 122 Copy all 68... - Page 140 Index Biometra TAdvanced Safety instructions 11 Tools 87 Settings Contact 112 Automatic user logout 90 Cycler Info 111 Beeper 91 Extended self-test 106 Date and time 89 Service info file 109 Display brightness 93 Touch screen Factory settings 95 Calibration 94 Network 91 User management 95 Screen calibration 94...
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