Motic Panthera Series Operation Manual
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Motic Panthera Series Operation Manual

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Panthera Series
Scientific Instrument
Operation Manual
The clear knowledge of this Instruction Manual is needed to operate Motic Panthera Series Microscopes at
maximum performance and to ensure safety at all specified operations. Please familiarize yourself with the use
of this microscope and pay special attention to the safety hints given in this manual. This Document is not subject
of a update routine, please download a newer version from the Motic website, if needed. Keep this instruction
manual in reach and easily accessible for future user reference. All Specifications, Illustrations and items in this
Manual are subject to changes.
WWW.MOTIC.COM
MOTIC HONG KONG LIMITED
E250223
If the equipment is used in a manner
not specified by the manufacturer,
the protection provided by the
Note
equipment may be impaired.

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  • Page 1 This Document is not subject of a update routine, please download a newer version from the Motic website, if needed. Keep this instruction manual in reach and easily accessible for future user reference. All Specifications, Illustrations and items in this Manual are subject to changes.
  • Page 2 English: Please familiarize yourself with the instruction manual provided in English. Versions in other languages are available for download at: http://www.motic.com/Panthera/Panthera_Eng_OP.zip...

  • Page 3: Table Of Contents

    TABLE OF CONTENTS General notes on Microscope Safety 1.1 General safety notes and instruction 1.2 Instrument safety, FCC and EMC conformity 1.3 Transporting, unpacking, storage of the microscope 1.4 Microscope disposal 1.5 Use of the microscope 1.6 Intended use of the microscope 1.7 Microscope warranty Nomenclature 2.1 Panthera E2 (Binocular Version)
  • Page 4 Usage of Microscope Components 5.1 Coarse and fine focusing 5.2 Coarse focus torque adjustment 5.3 Coarse focus quick stop 5.4 Stage upper limit stop adjustment 5.5 Interpupillary distance adjustment 5.6 Diopter adjustment 5.7 Adjustment of brightfield (Only for Panthera C2) 5.7.1 Full Kohler illumination 5.8 Use of aperture diaphragm...
  • Page 5 Troubleshooting Table 9.1 Optical 9.2 Electrical 10. Selection of the Power Supply Cord 11. Table 2 HAR Flexible Cord 12. Microscope Terminology...

  • Page 6: General Notes On Microscope Safety

    Please familiarize yourself with this operation manual before using the microscope. If additional information or support is needed, please contact Motic after sales Service. To ensure safe operation and optimal function of the instrument, please adhere to the precautions and warnings outlined in this manual.

  • Page 7: Instrument Safety, Fcc And Emc Conformity

    Risk of burn – Do not touch the lamp during or immediately after period of operation. Don’t pick the microscope up from the bottom during equipment operation. Proper handling of the microscope will ensure years of trouble-free service. If repair becomes necessary, please contact your Motic agency or our Technical Service directly.

  • Page 8: Transporting, Unpacking, Storage Of The Microscope

    Please always observe the following safety notes when using the microscope: Motic cannot assume any liability for applications other than its intended use, including the included modules and components. This includes service or repair work that is not carried out by authorized Motic service personnel.
  • Page 9 The operation of the microscope in explosion-risk environments is not allowed. Immersion oil can be harmful. Please only use Motic immersion oil and follow the safety instructions given. Immersion oil is to be used for microscope application only. Please do not...

  • Page 10: Intended Use Of The Microscope

    Motic must be notified of possible defects for steps to be taken to minimize damage. ● If notified of such a defect, Motic will evaluate the defect and if within warranty, rectify it at user’s discretion, either by repairing the microscope or by delivering a replacement.

  • Page 11: Nomenclature

    2. NOMENCLATURE 2.1 Panthera E2 (Binocular Version)
  • Page 12 Input Switch The switch on the back of the Panthera E2 is utilized to easily switch power supply methods for the microscope. Flicking the switch to the left (labeled as “USB 5v input”) powers the microscope with a built-in battery pack and flicking it to the right (labeled as “DC 5v input”) powers the microscope with the main power supply.

  • Page 13: Panthera C2 (Binocular Version)

    Panthera C2 (Binocular Version)
  • Page 14 Objective Lenses: Magnification N.A. W.D (mm) Plan UC 2X 0.05 Plan UC 4X 30.5 Plan UC 10X 0.25 17.4 Plan UC 20X 0.45 Plan UC 40X 0.65 Plan UC 60X 0.35 Plan UC 100X/1.25 Oil 1.25 0.16 Plan UC 100X/0.8 Working distance (WD): The distance from the cover glass top surface to the nearest point of the objective.

  • Page 15: Setting Up The Microscope

    ■ Light Tracer, digital light control ■ Trino, Plan UC optic ■ Rackless stage Please familiarize yourself with the instructions given in this operation manual. In case of unresolved questions, please contact Motic after sales service or consult Motic Webservices for further instructions.

  • Page 16: Assembling The Microscope

    4. ASSEMBLING THE MICROSCOPE 4.1 Verifying input voltage ● The automatic voltage selection works with a broad range of settings, please check the power rating of your country before the use of the microscope. • Always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards.

  • Page 17: Panthera C2

    4.3.2 Panthera C2 Panthera C2 Stage is fitted with a rackless stage providing more clearance for the user and avoids the possibility to scratch the user’s hand. ● Remove specimen holder for fast hand scanning of slides. ● Left-handed and right-handed operation stages are available as options. Left hand operation stages are available as options.

  • Page 18: Condenser

    4.6 Condenser ● Raise the stage by turning the coarse focus knob. ● Completely lower the condenser carrier by turning the condenser focus knob. (Fig. 4-1) ● Insert the condenser into the dovetail mount with aperture scale facing forward towards the user. Secure it with the condenser clamp screw.

  • Page 19: Eyepieces

    4.8 Eyepieces ● Use the same magnification eyepieces for both the eyes. ● To secure the eyepiece in the eyepiece sleeve, tighten the clamp screws. ● Twist the eyepiece (anti-clockwise or clockwise) with 20~30 degree (Fig. 6.1) and pull the eyepieces gently out when removing the eyepiece.

  • Page 20: Power Cord

     Filter selection: Filter Function ND2 (T=50%) ND4 (T=25%) For brightness adjustment in photomicrography ND16 (T=6.25%) Blue filter (colour balance filter) For routine microscopy and photomicrography For phase contrast and contrast adjustment with black and Green interference (546nm) white film For colour photomicrography of HE stained specimen with HE (didymium filter) tungsten type film...

  • Page 21: Usage Of Microscope Components

    5. USAGE OF MICROSCOPE COMPONENTS 5.1 Coarse and fine focusing (Fig. 10-1) ● Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope stand. ● The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs.

  • Page 22: Coarse Focus Quick Stop

    5.3 Coarse focus quick stop (Fig. 11) ● The coarse focus quick stop makes the stage can fixed at any position at which the specimen is in focus (i.e: by using the handle to lock the coarse focus knob.) ● With the specimen in focus, turn the handle to fix the knob.

  • Page 23: Interpupillary Distance Adjustment

    5.5 Interpupillary distance adjustment Due to every eye being uniquely different, adjustment is necessary to evoke the best performance from the microscope. Interpupillary distance adjustment enables the user to observe the specimen with both eyes without fatigue. ● Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective. ●...

  • Page 24: Diopter Adjustment

    5.6 Diopter adjustment Every human eye is different, so adjusting the instrument is necessary in order to ensure the best performance. ● Set the diopter on both eyepieces to the “0” position. ● Change to 10x magnification and bring the image of the specimen into focus using one eye only. ●...

  • Page 25: Adjustment Of Brightfield (Only For Panthera C2)

    5.7 Adjustment of Brightfield (Only for Panthera C2) 5.7.1 Full Kohler Illumination ● Place contrast-rich specimen slide into the slide holder (1) and swing in a 10x Objective Lens. ● Move any darkfield or phase contrast slider (2) out of the beam path, if equipped with it. ●...

  • Page 26: Use Of Aperture Diaphragm

    5.8 Use of aperture diaphragm ● The condenser aperture diaphragm adjusts the NA of the illumination system, which controls the resolution, contrast, depth of focus, and brightness of the image. ● Stopping it down will lower the resolution and brightness but increase the contrast and depth of focus.

  • Page 27: Illumination Brightness Adjustment

    5.11 Illumination brightness adjustment 5.11.1 Panthera E2: To adjust the brightness turn the light intensity control knob and observe the change in brightness until the desired level has been reached. (Fig. 15) There is no light manager or standby mode available in this model 5.11.2 Panthera C2 with light manager ●...

  • Page 28: Restore Factory Default For Intensity (Panthera C2)

    5.12 Restore factory default for intensity (Panthera C2) ● Turn the microscope off by switching the main switch on the backside of the microscope to off. Keep the intensity knob pressed and switch microscope on. Keep holding the intensity control knob for 10 seconds. The system has been reset to factory values.

  • Page 29: Standby Mode (Panthera C2)

    5.14 Standby mode (Panthera C2) Standby mode self activates after 15 minutes of idle time in order to conserve energy and protect the specimen from overexposure. ● To activate standby mode, double click the intensity knob or leave the microscope alone for 15 minutes.

  • Page 30: Photomicrographic Procedure

    6. PHOTOMICROGRAPHIC PROCEDURE ● To ensure vibration-free operation, set the microscope on a sturdy vibration-free table or a bench with a vibration-proof device. ● Pull the optical path selection lever of the trinocular eyepiece tube all the way out to its limit; the ratio of light entering the observation tube and standard phototube will be 20:80.

  • Page 31: Using Oil Immersion Objectives

    Oil immersion objectives are labelled as “Oil” and are to be immersed in oil between the specimen and the front of the objective. ● The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a refractive index of 1.515. ●...

  • Page 32: Care And Maintenance

    Store the objectives, eyepieces and filters in a container or desiccators with drying agent. ● Proper handling of the microscope will ensure years of trouble-free service. ● If repair becomes necessary, please contact your Motic agency or our Technical Service direct.

  • Page 33: Bulb Replacement

    Note: ● If the microscope is used in a manner not specified by the manufacturer, the warranty may be void. ● To avoid getting wet, do not use the microscope near water. 8.5 Bulb replacement ● The lamp and the lamphouse may become very hot during and after a period of operation. Risk of burn –...

  • Page 34: Panthera C2

    (Fig. 18-3) (Fig. 18-4) 8.5.2 Electrical Specifications: Panthera E2: Camera USB Output: 5V / 1A Input: AC100~240V, 50~60Hz 0.55A Bulb: 1W LED, color temperature: 5700K Panthera C2: Camera USB Output: 5V / 0.8A Input: AC100~240V, 50~60Hz 1.2A Bulb: 3W LED or 6V 30W Halogen LED module, high color temperature: 5500K~ 6000K LED module, low color temperature: 3000K ~ 3500K...
  • Page 35 9. TROUBLESHOOTING TABLE As you use your microscope, you may occasionally experience a problem. The troubleshooting table below contains the majority of frequently encountered problems and the possible causes. 9.1 Optical Problem Possible Cause Lamp not installed properly Lamp not centered Diffuser is in intermediate position Condenser not mounted correctly Condenser is not centered...
  • Page 36 Stage installed on inclined plane Unequal focus Specimen holder not fixed securely on stage Specimen not secured in position Lamp voltage is set too low Image tinged yellow Blue filter is not being used Slide is upside down Focusing is not possible with high magnification objectives Cover glass is too thick Slide is upside down...
  • Page 37 Motic’s microscopes are certified and tested for safety and environmental conformity. Only power supply cords which conform with the below listed certification marks and countries are applicable. CAUTION : Do not use non-approved power cord for Motic products, Motic can no longer warrant the electrical safety of the equipment.
  • Page 38 11. TABLE 2 HAR FLEXIBLE CORD Approval organizations and cordage harmonization marking methods Alternative marking Printed or embossed utilizing black-red-yellow harmonization marking (may thread (Length of color Approval organization be located on jacket or section in mm) insulation of internal wiring) Black Yellow Comite Electrotechnique Belge...
  • Page 39 12. MICROSCOPE TERMINOLOGY Abbe Condenser Magnification A two-lens sub-stage condenser located below The number of times by which the size of the the stage of a microscope and functions to collect image exceeds the original object. Another name light and direct it onto the object being examined. often used is “lateral magnification”.
  • Page 40 Diopter adjustment Resolving Power The adjustment of the eyepiece of a microscope A measure of an optical system's ability to that accommodates for the differences in produce an image which separates two points or eyesight for each individual observer. parallel lines on the object. Depth of Focus Resolution The axial depth of the space on both sides of the...
  • Page 41 Fax: 86-0592-562 7855 © 2002-2021 Motic China Group Co., Ltd. All rights reserved. Motic is a registered trademark and service mark of Motic China Group Co., Ltd. Microsoft Windows logo is a registered trademark of Microsoft Corporation. All other trademarks are the property of their respective owners.

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