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Note equipment may be impaired. The clear knowledge of this Instruction Manual is needed to operate Motic Panthera Series Microscopes at maximum performance and to ensure safety at all specified operations. Please familiarize yourself with the use of this microscope and pay special attention to the safety hints given in this manual.
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English: Please familiarize yourselves with the Instruction Manual provided in English language. Other Language versions are available as download on Motic web services under the Address: http://www.motic.com/Panthera/Panthera_Eng_OP.zip...
TABLE OF CONTENTS Genral notes on instrument safety General safety notes and Instruction Instrument safety, FCC and EMC conformity Transporting, unpacking, storage of the Instrument Instrument Disposal Use of the Instrument Intended use of the Microscope Instrument warranty Nomenclature Panthera S Panthera U / C / L / HD Setting up the instrument Operating environment...
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Digital parts (setup and operation) How to use QR-Link (For Panthera L / HD) ImagingOnDevice 5.2.1 Panthera Series Backside Connection Panel TeachingOnDevice (Panthera C / HD) 5.3.1 Panthera ImagingOnDevice realtime sharing ImagingOnDevice with QR-Link clients 5.3.2 ImageRecall Usage of microscope components...
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Care and maintenance Do not disassemble Cleaning the microscope 9.2.1 Lenses and filters 9.2.2 Cleaning of painted or plastic components Disinfecting the Microscope When not in use Bulb replacement 9.5.1 Replacing the 6V 30W halogen module or the two different LED module (For Panthera C / L / HD) 9.5.2 Electrical Specifications...
Please familiarize yourself with this Operation Manual before starting to use the Instrument. In case additional information or support is needed, please contact Motic after sales Service. To ensure safe operation and optimal function of the Instrument, strictly observe the precautions and warnings given in this Operation manual.
Risk of burn – Do not touch the lamp during or immediately after period of operation. Don’t pick the microscope up from the bottom during equipment operation. Proper handling of the microscope will ensure years of trouble free service. If repair become necessary, please contact your Motic agency or our Technical Service directly.
Please always observe the following safety notes when using the microscope: Motic cannot assume any liability for other applications then the intended use, including the included modules and components. This includes to service or repair work that is not carried out by authorized Motic service personnel.
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The operation of the instrument in explosion-risk environments is not allowed. Immersion Oil can be harmful. Please only use Motic immersion Oil and follow the safety Instructions given. I mmersion oil is to be used for Microscope application only! Please avoid skin...
If possible defects Motic must be notified immediately and steps be taken to minimize damage. ● If notified of such a defect Motic will evaluate the defect and if within warrant, rectify it at his discretion, either by repairing the instrument or by delivering a replacement.
2.2 Panthera U / C / L (Binocular Version) / HD...
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Full Kohler Illumination Fixed Kohler Illumination Objective Lenses: Magnification N.A. W.D (mm) Plan UC 2X 0.05 Plan UC 4X 30.5 Plan UC 10X 0.25 17.4 Plan UC 20X 0.45 Plan UC 40X 0.65 Plan UC 60X 0.35 Plan UC 100X/1.25 Oil 1.25 0.16 Plan UC 100X/0.8...
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Working distance WD: The distance from the cover glass top surface to the nearest point of the objective. Numerical aperture NA: The NA is a performance indicator. The higher NA, the higher the resolving power. It describes the ability of the lens to collect light under steep entry angles. Resolution: The ability to differentiate two points, or minimum distance under which two points can be differentiated.
3.SETTING UP THE INSTRUMENT Avoid placing the instrument in locations exposed to direct sunlight, dust, vibration, high temperature, high humidity and where it is difficult to unplug the power supply cord. 3.1 Operating environment ● Indoor use ● Altitude: Max 2000 meters ●...
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Panthera University ■ Light Tracer, digital light control ■ Bino, trino, Plan UC optic ■ Rackless Stage Panthera Classic ■ Bino, trino, Plan UC optic ■ Rackless Stage Panthera LifeScience ■ Light Tracer, digital light control ■ ImagingOnDevice ...
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■ QR-Link ■ ImageRecall ■ Similar to Panthera L, without Eyepiece Please familiarize yourself with the instructions given in this Operation Manual. In case of unresolved questions, please contact Motic after sales Service or consult Motic Webservices for further instructions.
4.ASSEMBLING THE MICROSCOPE 4.1 Verifying input voltage ● The automatic voltage selection works with a broad range of settings, please check the power rating of your country is admitted before the use of the Instrument under chapter specification. However, always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards.
4.3.2 For Panthera E / U / C / L / HD Panthera E, U, C, L, HD Stage is fitted with a Rackless providing more clearance for the user and avoid the possibility to scratch the users hand. ● Remove specimen holder for fast hand scanning of slides.
4.4 Specimen holder ● Attach the specimen holder, using the two mounting holes. 4.5 Objectives ● Lower the stage completely. Screw the objectives into the revolving nosepiece so that clockwise rotation of the nosepiece brings the next higher magnification objective into position. (Fig.3) 4.6 Condenser ●...
4.7 Eyepiece tube ● Loosen the eyepiece clamp screw (Fig.5-2). Insert the round dovetail mount on the eyepiece tube into the round dovetail mount on the microscope arm (Fig.5-1). Tighten the eyepiece tube clamp screw to secure the eyepiece tube in place. (Fig.5-2) (Fig.5-1) (Fig.5-2) 4.8 Eyepieces...
For Panthera C / L / HD: ● Remove the collector cover (Fig.8-1) and place the filter in the filter holder located around the field lens (Fig.8-2), screw back the collector cover (Fig.8-1), taking care that dust, dirt and fingerprints do not get on the filter and the field lens.
In case you do not have the Panthera APP yet, please scan the QR-Code on the backside Type Label to download Panthera APP in Android or IOS system, and get connected to the correct APP store via Motic servicing Servers. In case of any issue, please visit the Website http://www.motic.com/Panthera/app.html Connect the same network with Panthera.
5.2 ImagingOnDevice 5.2.1 Panthera Series Backside Connection Panel Panthera Series is providing the user with a unseen combination of build in Digital Capabilities. “Plug in your HDMI Screen, mouse and keyboard and start enjoy the simplicity of working with Motic Panthera Series.”...
● USB Keyboard, simply connect the Keyboard of your choice ● 1Gbit Lan connection port for high speed remote consultaion on Motic DssStore or simply network sharing ● Plug your personal USB stick to transport your imagery ● Connect your Panthera to the local Wifi or...
Panthera ImagingOnDevice stores relevant Data into the EXIF-Header of each Image being made. User can recall the same Illumination settings using the ImageRecall feature in order to allow a easy reconstruction of the before Experiment. Motic EXIF Header ■ Objective Lens Type with Specs ■ Brightness Settings...
6.USAGE OF MICROSCOPE COMPONENTS 6.1 Coarse and fine focusing (Fig.9-1) ● Focusing is carried out with the coarse and fine focus knobs at the left and right of the microscope stand. ● The direction of vertical movement of the stage corresponds to the turning direction of the focus knobs.
6.3 Coarse focus quick stop (Fig.10) ● The coarse focus quick stop makes the stage can fixed at any position at which the specimen is in focus i.e. by using the handle to lock the coarse focus knob. ● With the specimen in focus, turn the handle to fix the knob. ●...
6.5 Interpupillary distance adjustment Every human eye is different, to adjust the Instrument to best performance adjustment can be necessary. Interpupillary distance adjustment enables the user to observe the specimen with both eyes without fatigue ● Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective. ●...
6.6 Diopter adjustment Every human eye is different, to adjust the Instrument to best performance adjustment can be necessary. ● Set the diopter on both eyepieces to the “0” position. ● Change to 10x Magnification and the image of the specimen into focus using one eye only. ●...
6.7 Adjustment of Brightfield (Panthera C / L) 6.7.1 Full Kohler Illumination ● Place as contrast rich specimen slide into the slide holder (1) and swing in a 10x Objective Lens. ● Move any darkfield or phase contrast slider (2) out of the beam path, If equipped with it. ●...
6.8 Use of aperture diaphragm ● The condenser aperture diaphragm is to adjust the numerical aperture (N.A.) of the illuminating system, it serves the resolution of the image, contrast, depth of focus and brightness. ● Stopping it down will lower the resolution and brightness but increase the contrast and depth of focus.
6.11 Illumination brightness adjustment 6.11.1 Panthera S / E / C: To adjust the Brightness turn the Light Intensity Control Knob and observe the brightness change until the desired brightness has been reached. There is no Standby or sleep mode available in this model 6.11.2 Panthera U / L / HD: To adjust the Brightness, turn the Light Intensity Control Knob and observe the brightness change until the desired brightness has been reached.
6.13 Standby mode (For Panthera U / L / HD only) Standby mode self activates after 15 mins of idling time, in order to conserve energy, protect the specimen from overexposure. ● To activate Standby just double click the intensity knob or leave the microscope for 15 minutes, the standby mode turn on.
7.PHOTOMICROGRAPHIC PROCEDURE ● To ensure vibration free operation, set the microscope on a sturdy vibration free table or a bench with a vibration proof device. ● Pull the optical path selection lever of the trinocular eyepiece tube all of the way out to the limit, the ratio of light entering the observation tube and standard phototube will be 20:80.
Oil immersion objectives are labelled with the additional engraving “Oil” and are to be immersed in oil between the specimen and the front of the objective. ● The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a refractive index of 1.515. ●...
Store the objectives, eyepieces and filters in a container or desiccators with drying agent. ● Proper handling of the microscope will ensure years of trouble free service. ● If repair becomes necessary, please contact your Motic agency or our Technical Service direct.
Note: ● If equipment is used in a manner not specified by the manufacturer, the warranty may be void. ● To avoid getting wet, do not use the microscope near water. 9.5 Bulb replacement The lamp and the lamphouse become very hot during and after a period of operation. Risk of burn –...
(Fig.15-3) (Fig.15-4) 9.5.2 Electrical Specifications: Panthera S / E: Camera USB Output: 5V / 0.5A Input: AC100~240V, 50~60Hz 0.55A Bulb: 0.5W LED, color temperature: 5700K Panthera U: Camera USB Output: 5V / 0.5A Input: AC100~240V, 50~60Hz 0.55A Output: DC12V 1A Bulb: 3 W LED LED module, high color temperature: 5500K ~ 6000K LED module, low color temperature: 3000K ~ 3500K...
10.TROUBLESHOOTING TABLE As you use your microscope, you may occasionally experience a problem. The troubleshooting table below contains the majority of frequently encountered problems and the possible causes. 10.1 Optical Problem Possible Cause Lamp not installed properly Lamp not centred Diffuser is in intermediate position Condenser not mounted correctly Condenser is not centred...
Stage installed on inclined plane Unequal focus Specimen holder not fixed securely on stage Specimen not secured in position Lamp voltage is set too low Image tinged yellow Blue filter is not being us Slide is upside down Focusing is not possible with high magnification objectives Cover glass is too thick Slide is upside down...
Motic Instruments are certified and tested for safety and environmental conformity. Only power supply cords which are conform with below listed certification marks and countries are applicable. CAUTION : Do not use non-approved power cord for Motic products, Motic can no longer warrant the electrical safety of the equipment.
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Table 2 HAR Flexible Cord Approval Organizations And Cordage Harmonization Marking Methods Alternative Marking Utilizing Printed or Embossed Black-Red-Yellow Thred (Length Harmonization Marking (May be Approval Organization of color section in mm) located on jacket or insulation of internal wiring) Black Yellow Comite Electrotechnique Belge (CEBEC)
MICROSCOPE TERMINOLOGY Abbe Condenser Magnification A two-lens sub-stage condenser located below The number of times by which the size of the the stage of a microscope and functions to collect image exceeds the original object. Lateral light and direct it onto the object being examined. magnification is usually meant.
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Diopter adjustment Resolving Power The adjustment of the eyepiece of an instrument A measure of an optical system's ability to to provide accommodation for the eyesight produce an image which separates two points or differences of individual observers. parallel lines on the object. Depth of Focus Resolution The axial depth of the space on both sides of the...
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