Metrohm 6.5330.210 Manual
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Metrohm 6.5330.210 Manual

Metrohm 6.5330.210 Manual

Ic equipment: inline ultrafiltration-mipt
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IC equipment: Inline Ultrafiltration – MiPT (6.5330.210)
Manual
8.110.8039EN / 2019-10-25

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Summary of Contents for Metrohm 6.5330.210

  • Page 1 IC equipment IC equipment: Inline Ultrafiltration – MiPT (6.5330.210) Manual 8.110.8039EN / 2019-10-25...
  • Page 3 Metrohm AG CH-9100 Herisau Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 info@metrohm.com www.metrohm.com IC equipment IC equipment: Inline Ultrafiltration – MiPT (6.5330.210) Manual 8.110.8039EN / 2019-10-25...
  • Page 4 Technical Communication Metrohm AG CH-9100 Herisau techcom@metrohm.com This documentation is protected by copyright. All rights reserved. This documentation has been prepared with great care. However, errors can never be entirely ruled out. Please send comments regarding possible errors to the address above.

  • Page 5: Table Of Contents

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents Table of contents 1 Introduction Description of the IC equipment: Inline Ultrafiltration – MiPT ..................1 About the documentation ........... 2 1.2.1 Symbols and conventions ............3 2 Overview Parts of the IC equipment: Inline Ultrafiltration – MiPT ..................
  • Page 6 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents 6 Technical specifications Ultrafiltration cell (6.2729.110) ......... 33 Filtration membrane (6.2714.020) ........33 Liquid Handling Station (6.2841.120) ....... 33 Dosing unit 2 mL (6.3032.120) ......... 33 7 Accessories Index ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT...
  • Page 7 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures Table of figures Figure 1 IC equipment: Inline Ultrafiltration – MiPT – Overview ....... 2 Figure 2 IC equipment: Inline Ultrafiltration – MiPT – Parts ......4 Figure 3 Couplings, adapters and screws of the IC equipment: Inline Ultrafiltra- tion –...

  • Page 9: Introduction

    1 Introduction Description of the IC equipment: Inline Ultrafiltra- tion – MiPT The IC equipment: Inline Ultrafiltration – MiPT (6.5330.210) contains all the required accessory parts for the Inline Ultrafiltration and for the Metrohm intelligent Partial Loop Injection Technique (MiPT).

  • Page 10: About The Documentation

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1.2 About the documentation Figure 1 IC equipment: Inline Ultrafiltration – MiPT – Overview About the documentation This manual describes the installation of the IC equipment: Inline Ultrafil- tration – MiPT. CAUTION Please read through this documentation carefully before putting the equipment into operation.

  • Page 11: Symbols And Conventions

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction 1.2.1 Symbols and conventions The following symbols and formatting may appear in this documentation: Cross-reference to figure legend The first number refers to the figure number, the sec- ond to the instrument part in the figure. Instruction step Carry out these steps in the sequence shown.

  • Page 12: Parts Of The Ic Equipment: Inline Ultrafiltration - Mipt

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.1 Parts of the IC equipment: Inline Ultrafiltration – MiPT 2 Overview Parts of the IC equipment: Inline Ultrafiltration – MiPT Figure 2 IC equipment: Inline Ultrafiltration – MiPT – Parts ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT...

  • Page 13: Components Of The Ultrafiltration Cell

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview Figure 3 Couplings, adapters and screws of the IC equipment: Inline Ultrafiltration – MiPT Components of the ultrafiltration cell Figure 4 Ultrafiltration cell – Parts Stopper Upper chamber ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT...

  • Page 14: Connectors Of The Ultrafiltration Cell

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2.3 Connectors of the ultrafiltration cell Sealing ring Lower chamber Washers Screws For joining the upper and the lower part of the cell. Connectors of the ultrafiltration cell Figure 5 Ultrafiltration cell – Connectors Upper chamber Lower chamber Filtrate outlet Sample inlet Labeled OUT.

  • Page 15: Components Of The Liquid Handling Station

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview Components of the Liquid Handling Station Figure 6 Liquid Handling Station – Parts Cover Rinsing unit For the mixing vessel. Main body Rinsing unit inlet With magnetic stirrer dummy. Sealed with threaded stopper. Clamping fastener Base plate Platform Cable clip Waste connector...

  • Page 16: Mode Of Operation Of The Intelligent Partial Loop Injection

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Mode of operation of the intelligent Partial Loop Injection Technique Emptying the dosing unit At the beginning of the determination, the 2-mL dosing unit of the Dosino is emptied. Aspirating sample The peristaltic pump between the sample changer and the ultrafiltra- tion cell aspirates the sample.
  • Page 17 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Mode of operation of the intelligent Partial Loop Injection Technique Filling the sample loop a. The injection valve is switched to the Fill position. b. The 800 Dosino doses the injection volume from the transfer capillary to the 250 µL sample loop. The 800 Dosino doses the required ultrapure water from the ultrapure water bottle to the transfer capillary and the sample loop.

  • Page 18: Installing The Ultrafiltration Cell

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Installing the ultrafiltration cell 4 Installation Installing the ultrafiltration cell Figure 7 Connecting the ultrafiltration cell – Overview 4.1.1 Preparing the ultrafiltration cell Inserting the filtration membrane Required accessories Ultrafiltration cell (6.2729.110) ￭ Filtration membrane (6.2714.020) ￭ 5 mm hex key for IC Sample Processors (6.2621.070) ￭...
  • Page 19 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Plastic tweezers (6.2831.010) ￭ 1 Removing the stoppers Remove the 3 green stoppers. ￭ Turn the ultrafiltration cell around and place it on a table. The ￭ screws face upwards. 2 Removing the screws Loosen the screws with the hex key. ￭...
  • Page 20 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Installing the ultrafiltration cell Remove the upper chamber of the ultrafiltration cell. ￭ Remove the sealing ring. ￭ 4 Cleaning the ultrafiltration cell CAUTION Damage to the ultrafiltration cell Organic solvents (e.g. acetone) corrode and damage the ultrafiltra- tion cell material (PMMA).
  • Page 21 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Using the tweezers, take a new filtration membrane out of the ￭ package. Place the filtration membrane in a petri dish filled with ultrapure ￭ water and allow to hydrate for approx. 2 minutes. 6 Inserting the filtration membrane NOTICE The water-soaked filtration membrane must not dry out before it is inserted.

  • Page 22: Connecting The Ultrafiltration Cell

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Installing the ultrafiltration cell 8 Screwing the ultrafiltration cell together Screw the 5 screws with the washers in the ultrafiltration cell by ￭ hand first. Then firmly tighten them with the hex key in crosswise sequence. ￭ 4.1.2 Connecting the ultrafiltration cell Figure 8 Connecting the ultrafiltration cell...
  • Page 23 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Connecting the ultrafiltration cell Required accessories Ultrafiltration cell (6.2729.110) ￭ PEEK capillary, 0.5 mm ID / 70 cm (6.1831.160) ￭ PTFE capillary, 0.5 mm ID / 20 cm (6.1803.050) ￭ PTFE capillary, 0.97 mm ID / 5 m (6.1803.020) ￭...
  • Page 24 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Installing the ultrafiltration cell 3 Connecting capillaries to the ultrafiltration cell CAUTION Use only PVDF pressure screws (6.2744.000) to tighten capillaries to the ultrafiltration cell. PEEK pressure screws are too hard and can damage the ultrafiltra- tion cell material. Tighten the PTFE capillary (6.1803.050) to the inlet labeled IN of ￭...

  • Page 25: Inserting The Ultrafiltration Cell

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Tighten the free end of the PEEK capillary (6.1831.060) to Port 1 ￭ of the injection valve in the ion chromatograph (see manual for the ion chromatograph). Either tighten the free end of the PTFE capillary (6.1803.020) to ￭...
  • Page 26 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.1 Installing the ultrafiltration cell NOTICE The entire filtration system must be completely connected prior to the rinsing procedure. Rinsing the ultrafiltration cell 1 Settings in the software Immerse the needle of the Sample Processor into the rinsing solu- ￭...

  • Page 27: Equipping The Ultrapure Water Bottle

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Equipping the ultrapure water bottle Mounting the bottle cap Required accessories Eluent bottle / 2 L / GL 45 (6.1608.070) filled with ultrapure water ￭ Bottle cap for eluents and reagents GL 45 (6.1602.160) ￭ Adsorber tube for dosing unit (6.1619.000) ￭...

  • Page 28: Installing The Dosino

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.3 Installing the Dosino 3 Mounting the adsorber tube Fill the adsorber tube with some cotton and adsorber material. ￭ Place the adsorber tube onto the adapter. ￭ Insert the adapter into the SGJ opening of the eluent bottle cap. ￭...

  • Page 29: Figure 11 Mounting The Dosino

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation CAUTION Please read through the correct procedure in the manual for the 800 Dosino before you attach the Dosino to the dosing unit. 1 Attach the Dosino to the dosing unit (see 8.800.8002 manual for the 800 Dosino). Attaching the dosing unit to the ion chromatograph Required accessories Dosing unit 2 mL (6.3032.120)

  • Page 30: Connecting The Dosing Unit With The Ultrapure Water Bottle

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.3 Installing the Dosino 2 Attaching the Dosino to the holder Attach the Dosino with the dosing unit onto the Dosino holder. ￭ Fasten the dosing unit to the Dosino holder by tightening the ￭ thread adapter from below. 3 Connecting the Dosino to the ion chromatograph NOTICE The ion chromatograph must be switched off when the Dosino is...

  • Page 31: Connecting The Dosing Unit To The Injection Valve

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Figure 12 Mounting the FEP tubing Tighten one end of the FEP tubing in the M6 opening of the elu- ￭ ent bottle cap. Tighten the other end of the FEP tubing in Port 2 of the Dosino. ￭...

  • Page 32: Connecting The Dosing Unit To The Waste Container

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.3 Installing the Dosino Figure 13 Installing the transfer capillary 1 Mounting the adapter Tighten the adapter to port 1 of the dosing unit. ￭ 2 Installing the transfer capillary Tighten one end of the transfer capillary to the adapter using a ￭...

  • Page 33: Figure 14 Connecting To The Waste Container

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation Figure 14 Connecting to the waste container 1 Mounting the adapter Tighten the adapter (6.1808.280) to port 4 of the dosing unit. ￭ 2 Mounting the FEP tubing Connect one end of the FEP tubing to the adapter (6.1808.280). ￭...

  • Page 34: Exchanging The Sample Loop

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.4 Exchanging the sample loop Exchanging the sample loop Required accessories PEEK sample loop 250 µL (6.1825.290) ￭ 1 Replace the sample loop on the injection valve with the 250 µL sam- ple loop (see the manual for the ion chromatograph). Connecting the Liquid Handling Station Figure 15 Connecting the Liquid Handling Station –...
  • Page 35 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation NOTICE In order to keep dead volume to a minimum, make sure that the ￭ capillaries are as short as possible. The capillaries that are used for transferring the filtrate are thinner ￭ than the capillaries used for transferring the sample. To prevent the capillaries leading into the ion chromatograph from ￭...
  • Page 36 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4.5 Connecting the Liquid Handling Station 3 Connecting the capillaries to the pump tubing A PTFE capillary (6.1803.020) was connected to the bottle ￭ (6.1608.070) (see chapter 4.2, page 19). Tighten this PTFE capil- lary (6.1831.020) to the inlet of the pump tubing with yellow stoppers (6.1826.390) using a pressure screw (6.2744.070).

  • Page 37: Operation And Maintenance

    The following table lists some sample types that were filtered with the ultrafiltration cell (6.2729.110) and a filtration membrane (6.2714.020) with a pore size of 0.2 µm and subsequently analyzed on a Metrohm ion chromatograph. The concentration of the following seven anions was –...

  • Page 38: Selecting The Filtration Membrane

    Aqueous earth extracts 5.1.2 Selecting the filtration membrane You can apply existing sample preparation procedures to the Metrohm ultrafiltration cell (6.2729.110). If you use a different filtration membrane than the one supplied, please note that, even if the particle size is known, selecting a membrane with a suitable pore size does not automatically yield the desired results.

  • Page 39: Maintenance

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5 Operation and maintenance Except for this membrane, all membranes were from the same manufac- turer. Due to the lower filter thickness the retention capacity of filtration mem- branes may be lower than that of filters with the same pore size but a higher filter thickness.

  • Page 40: Maintenance Of The Dosing Unit (6.3032.120)

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 5.2 Maintenance Tighten the PTFE capillary (6.1803.050) to the inlet labeled IN of ￭ the ultrafiltration cell's lower chamber using a PVDF pressure screw (6.2744.000). Tighten the PTFE capillary (6.1803.020) to the outlet labeled OUT ￭ of the ultrafiltration cell's lower chamber using a PVDF pressure screw (6.2744.000).

  • Page 41: Technical Specifications

    ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 6 Technical specifications 6 Technical specifications Ultrafiltration cell (6.2729.110) Material PMMA (poly(methyl methacrylate)) Solvent compati- Water or water-ethanol mixture (70:30) bility (no other organic solvents) Cell volume 240 µL (each from inlet opening to outlet opening) Filtration membrane (6.2714.020) Pore diameter 0.2 µm Membrane diame-...

  • Page 42: Accessories

    Downloading the accessories list 1 Enter https://www.metrohm.com/ into your Internet browser. 2 Enter the article number (e.g. 6.5330.210) into the search field. The search result is displayed. 3 Click on the product. Detailed information regarding the product is shown on various tabs.
  • Page 43 ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Index Index Select ......... 30 Bottle Replace Service life ......29 Equip ........19 Filtration membrane ... 31 Bottle cap Rinse Mount ........ 19 Injection valve Ultrafiltration cell ....17 Connect ......23 Insert Capillary connection ....26 Sample loop Ultrafiltration cell ....

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