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VWR
PerfectBlue
Systems Twin S, M, L & ExW S
European Catalogue Numbers:
Twin S
Twin S w/o gel caster
Twin M
Twin M w/o gel caster
Twin L
Twin L w/o gel caster
Twin ExW
Twin ExW w/o gel caster
Version:
1
Issued:
10,04,2018
™
Vertical Dual Gel
Instruction Manual
700-0931
700-0937
700-0955
700-0961
700-1080
700-1205
700-0980
700-0986
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Table of Contents
Related Manuals for VWR PerfectBlue Twin S
Summary of Contents for VWR PerfectBlue Twin S
- Page 1 ® ™ PerfectBlue Vertical Dual Gel Systems Twin S, M, L & ExW S Instruction Manual European Catalogue Numbers: Twin S 700-0931 Twin S w/o gel caster 700-0937 Twin M 700-0955 Twin M w/o gel caster 700-0961 Twin L 700-1080 Twin L w/o gel caster 700-1205 Twin ExW...
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Page 2: Table Of Contents
Tris-Tricine SDS-PAGE Troubleshooting Technical Support and Ordering Information PerfectBlue™ Dual Gel System Twin S PerfectBlue™ Dual Gel System Twin M PerfectBlue™ Dual Gel System Twin L PerfectBlue™ Dual Gel System Twin ExW S Power Supplies VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 3: Legal Address Of Manufacturer
VWR warrants that this product will be free from defects in material and workmanship for a period of three (3) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period. -
Page 4: Packaging List
1 SpacerPlacer for easy spacer alignment 1 SpacerPlacer for easy spacer alignment Twin ExW S (700-0980) Twin L (700-1080) as the 700-0986 but including casting base as the 700-1205 but including casting base VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 5: System Overview
500 V 15 - 75 mA 60 - 180 min 700-1205 700-0980/ Twin ExW S 20 x 10 cm 650 ml up to 500 V 30 - 45 mA 30 - 90 min 700-0986 VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 6: Short Instruction
Invert casting base and turn out the cams. Wet the silicone mats with e.g. water or buffer to prolong their life span. Place UBC on the gaskets and turn the handles at the same time, half a turn. VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 7: Assembling The System And Electrophoresis
Place safety lid onto the unit and start the run. After the run is completed turn off the power supply and remove lid. Lift UBC from LBC, loosen side clamps to remove gel assembly for further analysis. VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 8: General Instructions
4. After aligning and clamping the glass plate assembly the UBC will be removed and the casting base has to be turned around so that the black silicone mats are facing upward. Wet the silicone mats with e.g. water or buffer to prolong their life span. VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 9: Assembling The System And Electrophoresis
6 mm, the outer diameter is 9 mm. Note the marks indicating 'in' and 'out' at the adapters to assure the right flow direction. Water flow should not exceed 2 L per minute for the water tap and 12 L per minute for the recirculator. VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 10: Cleaning
Each part should get completely dry before storage. Do not use ethanol or other organic solvents to clean acrylic products, because organic solvents cause acrylic to 'craze' or crack! VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 11: Required Materials And Recipes
Required Materials and Recipes Required Gel Volumes These volumes have been calculated for glass plates and spacers available at VWR. PerfectBlue™ Dual Gel System Twin S Twin M Twin ExW S Twin L 0.8 mm Spacer 8 ml 18 ml... -
Page 12: Tris-Tricine Sds-Page
3 M Tris-HCl (pH 8.45) 0.62 1.24 1.86 3.10 6.20 10 % SDS 0.02 0.03 0.06 10 % Ammonium persulfate (APS) 0.005 0.01 0.015 0.025 0.05 TEMED 0.001 0.001 0.002 0.005 0.01 All amounts in ml VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 13: Troubleshooting
Leakage of buffer through Do not move spacers after the gel polymerized. Make sure gel sides/spacers that the glass plates are seated firmly against the gasket. Always use combs and spacers of the same thickness. VWR PerfectBlue Twin ver 1 10,5,2018... - Page 14 Always use combs and spacers of spacers. the same thickness. VWR PerfectBlue Twin ver 1 10,5,2018...
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Page 15: Technical Support And Ordering Information
Technical Support and Ordering Information For technical questions and more detailed information on VWR’s products please contact your local VWR office or visit the VWR website at vwr.com. Visit the VWR website at vwr.com... -
Page 16: Perfectblue™ Dual Gel System Twin M
20 x 20 cm (W x L), with casting base 700-1080 Casting base L including silicone gaskets and cams 700-1087 Gel Caster L complete system for sealing up to 2 gels 700-1093 VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 17: Perfectblue™ Dual Gel System Twin Exw S
1.5 mm 25 teeth 80 µl* 700-0978 0.8 mm 10 teeth 150 µl* 700-0968 0.8 mm 15 teeth 90 µl* 700-0973 0.8 mm 20 teeth 60 µl* 700-0975 0.8 mm 25 teeth 40 µl* 700-0977 VWR PerfectBlue Twin ver 1 10,5,2018... -
Page 18: Power Supplies
3 pieces 700-0994 * volumes are calculated for a well length of 14 mm Power Supplies Do not hesitate to contact us for advice on which Power Supply is most suitable for your application. VWR PerfectBlue Twin ver 1 10,5,2018... - Page 19 Your Distributor Austria Germany Poland VWR International GmbH VWR International GmbH VWR International Sp. z o.o. Graumanngasse 7 Hilpertstraße 20a Limbowa 5 1150 Vienna D - 64295 Darmstadt 80-175 Gdansk Tel.: +43 01 97 002 0 Freecall: 0800 702 00 07 Tel.: +48 058 32 38 200...