THORLABS EDU-OMC1 User Manual
  1. Manuals
  2. Brands
  3. THORLABS Manuals
  4. Laboratory Equipment
  5. EDU-OMC1
  6. User manual
THORLABS EDU-OMC1 User Manual

THORLABS EDU-OMC1 User Manual

Optical microscopy course
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Optical Microscopy Course
EDU-OMC1
EDU-OMC1/M
User Guide
Rev C, October 1, 2020

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the EDU-OMC1 and is the answer not in the manual?

Questions and answers

Related Manuals for THORLABS EDU-OMC1

Summary of Contents for THORLABS EDU-OMC1

  • Page 1 Optical Microscopy Course EDU-OMC1 EDU-OMC1/M User Guide Rev C, October 1, 2020...

  • Page 3: Table Of Contents

    Software Installation and Digital Materials ..............50 Software Installation ....................50 7.1.1 ThorCam Camera Software 7.1.2 Thorlabs OSA Spectrometer Software 7.1.3 Additional Software Supplied Digital Materials ..................52 Chapter 8 Regulatory ........................53 Chapter 9 Thorlabs Worldwide Contacts ..................54...

  • Page 5: Warning Symbol Definitions

    Optical Microscopy Course Kit Chapter 1: Warning Symbol Definitions Chapter 1 Warning Symbol Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal...

  • Page 6: Safety

    Optical Microscopy Course Kit Chapter 2: Safety Chapter 2 Safety CAUTION After the QTH10 lamp warms up, the housing will reach a temperature of up to 55 °C. Be careful not to touch the lamp housing during operation. CAUTION The kit includes an IR LED (LED940E), which does not emit in the visible range. The LED emission can be checked with most cameras (camera chips are often IR sensitive).

  • Page 7: Brief Description

    They have been substantially updated and revised as part of a multiyear collaboration with Thorlabs in order to make the material more widely available; in the process, substantial new hardware has been developed to improve and/or simplify offering of the material.
  • Page 8 Optical Microscopy Course Kit Chapter 3: Brief Description User Guide Lab Notes Course Notes Instructor Notes Figure 2 Overview of the included documentation. A total amount of about 500 pages gives the physical background, step-by-step instructions of the assembly and lab procedure, as well as tips for tutoring the course.

  • Page 9: Setup Overview

    Optical Microscopy Course Kit Chapter 3: Brief Description • Lectures on Lab # • Provides (well in advance): Lecturer Course Notes and Lab Notes for entire course Do experiments in Lab # • Attend Lecture on Lab # • Refer to Lab Notes # for •...
  • Page 10 Optical Microscopy Course Kit Chapter 3: Brief Description Each experiment includes multiple evaluation tasks for students to perform, giving them experience with both qualitative observation and quantitative comparison of data with theory. Figure 4 Overview of the components on the optical rail: 1) Camera, 2) Objective, 3) Sample, 4) Condenser and Aperture Stop, 5) Neutral Density Filter Wheel, 6) Green Filter, 7) Field Stop, 8) Collector Lens, and 9) Lamp with IR Filter Figure 5 Photo of a second camera imaging the back focal plane of the objective to teach image...

  • Page 11: Lab Notes And Course Notes Overview

    Optical Microscopy Course Kit Chapter 3: Brief Description Lab Notes and Course Notes Overview This section provides a quick run through of the content of each lab with graphical illustrations of the key features. Lab Content Introduction to Optical Imaging (I) Handling Cameras and Using Camera Software Controls Imaging and Lenses;...
  • Page 12 Optical Microscopy Course Kit Chapter 3: Brief Description The Abbe Theory of Image Formation (I) Imaging the BFP: Setting Up a BFP-Imaging Camera Diffraction and the BFP Spatial Filtering in the BFP The Abbe Theory of Image Formation (II) The Modulation Transfer Function (MTF) o Incoherent MTF o Coherent MTF...

  • Page 13: Supplementary Documentation

    Assembly, Kohler, and Back Focal Plane videos at the www.thorlabs.com/OMC video tab both for a quick rundown of the assembly steps and to assist in setting up the system for the first time. Be aware that using these videos will skip most of the learning experience;...

  • Page 14: Kit Components

    Optical Microscopy Course Kit Chapter 4: Kit Components Chapter 4 Kit Components All kit components are shown below, broken out by subassembly for convenience as you set up. Unless otherwise noted, in cases where the metric and imperial kits contain parts with different item numbers, metric part numbers and measurements are indicated by parentheses.
  • Page 15 Optical Microscopy Course Kit Chapter 4: Kit Components General Tools and Optomechanics 2 x R2T Thin Slip-On Post Collar for 1 x CCS200 (CCS200/M) Ø1/2" Posts, 5 Pack Compact Spectrometer, 200 nm - 1000 nm, Software and Fiber Patch (10 Post Collars Total) Cable Included 6 x TR4 (TR100/M) 16 x TR3 (TR75/M)
  • Page 16 Optical Microscopy Course Kit Chapter 4: Kit Components 1 x MS10UW2 1 x CG00C2 Microscope Slides, 1 mm Thick, Cover Glasses, #0 Thickness, 1 x HKTS-3/16 (HKTS-5M) White Marking Region, Pack of 200 22 x 22 mm, Pack of 200 3/16"...
  • Page 17 Optical Microscopy Course Kit Chapter 4: Kit Components Lamp 1 x SM2A6 1 x FGB37M Adapter with External SM2 Mounted Ø25 mm BG40 Colored 1 x QTH10 (QTH10/M) Threads and Internal SM1 Glass Bandpass Filter, Quartz Tungsten-Halogen Lamp Threads 335 - 610 nm 1 x HSLT2 1 x SM1CP2 Passive-Heat-Sink SM1 Lens...
  • Page 18 Optical Microscopy Course Kit Chapter 4: Kit Components Spectral Filtering 1 x Colored Glass 2 x TRF90 (TRF90/M) 1 x ER1 Bandpass Filter 90° Flip Mount for Ø1" Filters and Ø6 mm Cage Assembly Rod, Ø25 mm VG9, 3 mm Thick Optics 1"...
  • Page 19 Optical Microscopy Course Kit Chapter 4: Kit Components Sample Stage 1 x Sample Stage Adapter 1 x MS1S + Metric Micrometer 1 x XYF1 (XYF1/M) Connects MS1S (MS1S/M) to (MS1S/M) XY Mount for 1" - 3" Rectangular XYF1 (XYF1/M) 6.5 mm Travel Single-Axis Optics Translation Stage with Side- Mounted Metric Micrometer...
  • Page 20 Optical Microscopy Course Kit Chapter 4: Kit Components Nikon Objective 1 x SM1A12 1 x N10X-PHE External SM1 to Internal M25 10X Nikon Phase Contrast Thread Adapter Objective, Apodized Dark Low (for Nikon Objective) Phase Type, 0.25 NA, 6.2 mm WD Beamsplitter 1 x LMR2 (LMR2/M) 1 x EBP2...
  • Page 21 Optical Microscopy Course Kit Chapter 4: Kit Components Fluorescence Emission Filter 1 x FELH0550 1 x SM1L05 550 nm Premium Longpass Filter 1 x SM1CP1 Ø1" Lens Tube, 0.50" Long (Fluorescence Emission Filter) Internally SM1-Threaded End Cap Back Focal Plane Camera 1 x SM1V10 Ø1"...
  • Page 22 Optical Microscopy Course Kit Chapter 4: Kit Components LED Mounts 4 x SMR05 (SMR05/M) 3 x LEDMT1E 1 x LEDMT1F Ø1/2" Fixed Lens Mounts with No LED Mount, 51 Ω LED Mount, 62 Ω Retaining Lip 1 x LED528EHP 2 x LED405E 2 x LED631E Epoxy-Encased LED, 525 nm, Epoxy-Encased LED, 405 nm,...
  • Page 23 Optical Microscopy Course Kit Chapter 4: Kit Components Accessories 1 x Aluminum Foil Roll 1 x Flexible Ruler 300 mm Wide, 10 m Long, 15 µm Thick 1 x Highlighter Set Pack of 4 Colors 1 x Small LED Flashlight USB Rechargeable 1 x LED Overhead Light 1 x Clear Nail Polish...
  • Page 24 Optical Microscopy Course Kit Chapter 4: Kit Components Samples 1 x T14792 Invitrogen Molecular Probes 1 x H&E Stained Histology Slides: TetraSpeck Fluorescent Carolina Biosciences No. 317120, 1 x R1L3S11P Microspheres Size Kit 317798, 318090, 316542 Positive Combined Resolution and (Mounted on Slide) Distortion Test Target, 3"...

  • Page 25: Setup And Adjustment

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Chapter 5 Setup and Adjustment This chapter discusses how to assemble the various components and explains how to set up and adjust the microscope. Screws are either contained with the components or can be found in the bag labeled Hardware Kit. Breadboard and Rail Assembly Attach the RDF1 damping feet to the bottom side of the 8"...

  • Page 26: Optics Subassemblies

    The following section shows how to assemble the optical components. Handling lenses and optics in general requires the use of powder-free gloves. You can find these in all sizes within Thorlabs’ Optics Lab Supplies Included in the kit is a benchtop organizer to store the optics and balldrivers. The balldrivers can also be used with the through holes as a lever on the posts to tighten the posts to the optics holders.

  • Page 27: Halogen Lamp Assembly

    Remove the power supply if attached and remove the end cap to access the filament socket. The cap can be unscrewed by hand using light pressure, or with special tools from Thorlabs, such as the spanner wrench Item #’s SPW909 or SPW801 (sold separately).
  • Page 28 Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Loosen the setscrew for the bulb socket and remove it from the lamp. Insert the bulb into the socket with gloves and put the socket back into the lamp, as shown below. Adjust the socket and tighten the setscrew so that the filament of the bulb is in the center of the lamp, at the cylinder axis and perpendicular to it.

  • Page 29: Collector Lens Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Add aluminum SM1CP2 end cap (not the plastic end caps) to the open side of the heat sink. Mount the lamp on a TR3 (TR75/M) post. Place the lamp's post in the benchtop organizer. The organizer also has through holes to hold the balldrivers included in the kit.

  • Page 30: Field Stop

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Add the “F = 35 mm PCX” (PCX: plano-convex lens) label to the top of the lens mount. Place the mount into the benchtop organizer for storage. 5.2.3 Field Stop The ID25 (ID25/M) field stop iris is preassembled as shown. A label “Field Iris” is provided to attach to either the field stop post or, at a later stage, on the XT34TR1 (XT34TR1/M) rail carrier it goes into during the assembly of the kit.
  • Page 31 Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Repeat steps 2 and 3 with ND10B, ND13B, ND20B and ND30B filter subsequently in this order and label them accordingly. Metallized sides of all filters should face the same direction. Add the label “Open” to the remaining filter wheel optic mount (the one with no filter installed). Attach the 1/4"-20 (M6) setscrew [1/2”...

  • Page 32: Flip Mount Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment 5.2.5 Flip Mount Assembly Use the SPW602 spanner wrench to remove the retaining rings from the two TRF90 (TRF90/M) 90° flip mounts. The mount has various locking and mounting screws shown below. A 0.05" (1.3 mm) hex key is provided with the item.

  • Page 33: Condenser Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Screw a TR3 (TR75/M) post into one of the TRF90 (TRF90/M) flip mounts with the FGV9 filter installed. Place the flip mount into the benchtop organizer for storage. Place the hex key in the plastic bin. 5.2.6 Condenser Assembly Screw the TR3 (TR75/M) post to the SM1RC (SM1RC/M) slip ring and set it aside.

  • Page 34: Sample Stage Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Insert the SM1M10 lens tube into the previously assembled SM1RC (SM1RC/M) slip ring and tighten it with the locking screw on top of the slip ring. Locking Screw Unscrew the retaining ring from the SM1V10 adjustable lens tube and insert the AC254-050-A lens with the flat side (lower curvature) towards the inner lip of the SM1V10.
  • Page 35 Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Dowel Pins Use two 8-32 (M4) cap screws, 1/2" (12 mm) long, and screw the MS1S stage adapter to the MS1S-SP (MS1S/M) stage as shown in the image. The dowel pins are tight fit and will make a precise orthogonal alignment.

  • Page 36: Pcx Objective Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment AP8E25E (AP6M4M) Adapter Screw, 1/4"-20 to 8-32 (M6 to M4) Remove the setscrew from the TR2 (TR50/M) post and screw the post upside down on the bottom of the MS1S-SP (MS1S/M) stage. To avoid damage to the ball-bearings, avoid strain on the stage during the attachment by holding the bottom part of the stage while tightening.

  • Page 37: Achromatic Objective Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Use the SM1RRC extra-thick retaining ring to fix the lens into position. The retaining ring has two sides. Use the thickest part of the retaining ring against the lens. Screw the TR3 (TR75/M) post into the LMR1 (LMR1/M) optic mount. Put the adhesive label “F = 25 mm PCX”...

  • Page 38: Back Focal Plane (Bfp) Beamsplitter Assembly

    (best would be plastic) tool to avoid scratching the optic. The EBP2 is an economy beam splitter with the two sides parallel. To minimize internal reflections, wedged beam splitters, e.g. the Thorlabs BSS16, can be used (https://www.thorlabs.com/thorproduct.cfm?partnumber=BSS16). We purposefully use Page 34...

  • Page 39: Microscope Objective (Nikon)

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Attach the TR3 (TR75/M) post Add the “EPB2 70-30” adhesive label to the top of the mount. Place the assembly into the benchtop organizer for storage. 5.2.11 Microscope Objective (Nikon) Remove the retaining ring from the LMR1 (LMR1/M) optic mount. Screw the SM1A12 M25 thread adapter into the optic mount.

  • Page 40: Polarization Mount Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Place the assembly into the benchtop organizer for storage. 5.2.13 Polarization Mount Assembly Unscrew the retaining ring from the LMR1 (LMR1/M) optic mount. Take one polarizer from the box. The polarizers have a flattened edge which is parallel to the transmission axis.

  • Page 41: Fluorescence Filter Assembly

    Trim away the excess tape. There are also inexpensive, purchasable ground-glass (not to mention higher-performance engineered) diffusers available from Thorlabs; however, learning to prototype with simple materials is a valuable skill, and one we wanted to emphasize for students in the class.

  • Page 42: Led And Multi-Purpose Mount Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Place the assembly into the benchtop organizer for storage. 5.2.16 LED and Multi-Purpose Mount Assembly Screw four SMR05 (SMR05/M) optic mounts onto four TR4 (TR100/M) posts. Use the arrow markings on the barrel of the LEDMT1F LED mount (62 Ω resistor) as a template to cut the leads of the LED631E LED to the appropriate length.

  • Page 43: Sample Camera Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Place all of the LED assemblies into the benchtop organizer for storage. 5.2.17 Sample Camera Assembly Remove the retaining ring from the SM1V10 adjustable lens tube. Add the “F = 150mm Ach” label to the adjustable lens tube. Insert the AC254-150-A achromatic lens with the larger radius of curvature (flatter) side towards the inner lip and fix the lens in place with the retaining ring.
  • Page 44 Insert the SM1L30 lens tube attached to the camera into the SM1RC (SM1RC/M) slip ring, and tighten the slip ring such that the camera is horizontally aligned (Thorlabs Zelux logo visible on the top side of the camera). The slip ring position is flexible; approximately halfway down the combined SM1 tubes is a good initial placement.

  • Page 45: Back Focal Plane (Bfp) Camera Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment 11. Put a lens cap (SM1EC2B) on the SM1V10 to cover the lens. 12. Place the assembly into the benchtop organizer for storage. 13. Two additional 1.00" (25.4 mm) SM1L10 lens tubes and one 0.5" (12.5 mm) SM1L05 lens tube are available –...

  • Page 46: 780 Nm Longpass Filter Assembly

    Insert the SM1L10 lens tube on the camera into the SM1RC (SM1RC/M) slip ring, and tighten the slip ring such that the camera is horizontally aligned (Thorlabs Zelux logo visible on the top side). The slip ring should be near the middle of the SM1L10 on the camera (see figure below).

  • Page 47: Multi-Purpose Mount Assembly

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Remove the retaining ring from the LMR1 (LMR1/M) and screw the preassembled FGL780M longpass filter on the optic mount. Add the label “FGL780 LP” to the mount. Place the assembly into the benchtop organizer for storage. 5.2.20 Multi-Purpose Mount Assembly Remove the setscrew from a TR4 (TR100/M) post.

  • Page 48: Zero Order Blocking Mask

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment 2 - 3 mm Screw the SM1SMA fiber adapter against the retaining ring and place the mount into the benchtop organizer for storage. 5.2.22 Zero Order Blocking Mask Remove the retaining ring from an SM1L03 0.3" (7.6 mm) lens tube. The Zero-Order Blocking Mask consists of an UV printed dot on a glass substrate.

  • Page 49: Thin Slip-On Post Collars

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Thin Slip-On Post Collars The R2T slip-on post collars are an important tool to secure the optics height during vertical alignment. They can be used with the included setscrews or with the additionally provided thumbscrews in the hardware kit. The thumbscrews can be a great help, providing quick access to height adjustments without the need of a hex wrench.

  • Page 50: Overhead Lamp And Led Flashlight

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Overhead Lamp and LED Flashlight Included in the kit is an LED Overhead Light (Daffodil ULT300). It can be clamped to a monitor, giving just enough light for students to take notes (and read the lab procedures) during the labs, but not interfering with low light microscopy measurements (or of other students).

  • Page 51: Plastic Bin And Spare Parts

    Optical Microscopy Course Kit Chapter 5: Setup and Adjustment Plastic Bin and Spare Parts The following items should be placed in the plastic bin before the start of the course: Flexible Ruler Tape Dispenser 2 x Additional Ø1.00" (Ø25.4 mm) Lens Tubes (See Section 5.2.17) 1 x Additional Ø1/2"...

  • Page 52: Samples

    Abbe slides used to introduce his theory (and Zeiss microscopes) c. 1900. Despite their pedagogical utility, the Abbe slide elements have been out of production for decades until Thorlabs agreed to make this target for the kit. The targets are made by plating vacuum-sputtered, low reflectivity chrome with an optical density of ≥3 at...
  • Page 53 Optical Microscopy Course Kit Chapter 6: Samples Tissue Samples H&E stained histology slides: #317120 Human Myocardial Infarct Microscope Slides #317798 Human Adenocarcinoma of Lung #318090 Human Metastatic Carcinoma to Liver #316542 Human Prostate Gland – Young Storage requirements: protect the slides from light when not in use, e.g.

  • Page 54: Software Installation And Digital Materials

    CCS Spectrometer Installation and Quick Start Manual CCS Spectrometer Manual A quick video tour through the settings needed for the kit can be found on the EDU-OMC1(/M) product page on http://www.thorlabs.com/OMC website and in the Lab Notes material. 7.1.2...

  • Page 55: Additional Software

    Zelux CS165XX Camera Manual ThorCam Camera Series Software Manual A quick video tour through the settings needed for the kit can be found on the EDU-OMC1(/M) product page on http://www.thorlabs.com/OMC website and in the Lab Notes material. The Camera has a status LED indicator: Blue: Connected to USB 3.0 Port (~ 35 FPS in full resolution)

  • Page 56: Supplied Digital Materials

    Optical Microscopy Course Kit Chapter 7: Software Installation and Digital Materials Supplied Digital Materials The included USB Stick includes the following material which can also be found on the product website: Manual – How to Set Up the Rail and Optical Components Lab Notes –...

  • Page 57: Regulatory

    Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.

  • Page 58: Thorlabs Worldwide Contacts

    Optical Microscopy Course Kit Chapter 9: Thorlabs Worldwide Contacts Chapter 9 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to-date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc.
  • Page 60 www.thorlabs.com...

This manual is also suitable for:

Edu-omc1/m

Table of Contents