What You Will Need - Agilent Technologies InfinityLab LC Series User Manual

3 Running an Isocratic Analysis

What You Will Need

What You Will Need

Instruments
Column
Solvents
Sample
The descriptions are based on Agilent 1100/1200 systems. The Agilent 1290
NOTE
Infinity System may have other requirements on the setup and material.
G4208A User's Guide
Agilent Series isocratic, binary or quaternary pump, an autosampler and a
UV- detector.
A 125 mm × 4.0 mm Hypersil ODS, 5 μm (Agilent Technologies part number
7982618- 564).
For the isocratic pump, a solvent mixture of LC grade bidistilled water (35 %)
and acetonitrile (65 %).
The Agilent Technologies isocratic standard sample (Agilent Technologies
part number 01080- 68704). This contains 0.15 wt.% dimethylphthalate,
0.15 wt% diethylphthalate, 0.01 wt.% biphenyl and 0.03 wt.% o- terphenyl
dissolved in methanol.
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