Daily Cleaning - R-Biopharm KOBRA CELL Instruction Manual

R-Biopharm Rhone Ltd

Daily Cleaning

6.2
The HPLC system can be left running overnight at
a reduced flow rate (e.g. 0.1 ml/min) and with the
fluorescence detector and KOBRA® CELL power
source turned off, if necessary. Alternatively, a better
practice to prolong the life of the system is to clean
each day, as follows:
1. Turn off KOBRA® CELL power pack and
fluorescence detector.
2. Switch off HPLC pump.
3. Disconnect the KOBRA® CELL from the HPLC
system and reconnect the HPLC column directly
to the fluorescence detector.
4. Change the mobile phase to 5 % solvent and put
the HPLC column discharge to waste.
5. Switch on the HPLC pump and flush the system
for at least 30 minutes before switching off the
system.
HPLC Column
Out
'Fingertight'
Fittings
'Union' Fittings
Fig 6. KOBRA CELL Switched On for Analysis
®
6. Manually flush the KOBRA® CELL through with
distilled water several times using a syringe.
7. Store the KOBRA® CELL overnight with water
inside the cell by closing off both ports on each
housing using the storage plugs and capillary
tubing supplied with the cell.
8. The next day, change the mobile phase from
5 % solvent back to the normal mobile phase of
potassium bromide and nitric acid, and flush the
HPLC system through for at least 30 minutes
before re-connecting the KOBRA® CELL.
Caution: 100 % acetonitrile will damage the
KOBRA® CELL membrane.
9. Reconnect the KOBRA®
KC # 123
KC # 123
Solvent waste
16
CELL (Fig. 6).
Detector
Inlet
Outlet