Related Manuals for R-Biopharm KOBRA CELL
Summary of Contents for R-Biopharm KOBRA CELL
- Page 1 R-Biopharm Rhône Ltd KOBRA® CELL Art. No. K01 Instruction Manual R-Biopharm – for the safety of your analysis.
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Page 3: Table Of Contents
Contents Introduction to the KOBRA® CELL ....................4 Intended Use ..........................4 Derivatisation Reaction ........................5 KOBRA® CELL Contents ........................6 Contents ............................6 Reagents Required ......................... 6 Accessory Products ........................6 Hazards ............................6 Decontamination ........................... 6 Assembly of the KOBRA® CELL ......................7 Components .......................... - Page 4 R-Biopharm Rhone Ltd Troubleshooting ..........................17 No Peaks Observed ........................17 Reduced / No Aflatoxin B1 and G1 Peaks Observed ..............18 Abnormal Peaks Observed ......................19 Error Light Observed on Power Pack .................... 20 Leakage from KOBRA® CELL ......................20 KOBRA® CELL Causing High Pressure on the HPLC ............... 20 Storage Plug Broken Off or Lodged in KOBRA®...
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Page 5: Introduction To The Kobra® Cell
Introduction to KOBRA CELL ® Intended Use Confirmation of the presence of aflatoxins in a Post-column derivatisation with iodine also has sample by HPLC requires derivatisation of aflatoxins some limitations including the need for a second B1 and G1 in order to enhance their natural pump, water bath or oven which can be expensive. -
Page 6: Derivatisation Reaction
R-Biopharm Rhone Ltd Derivatisation Reaction The aflatoxins and the mobile phase enter the KOBRA® CELL and the electrochemical reaction + Br occurs generating the reactive form of bromine. The reaction between the reactive bromine and the Aflatoxin B1 aflatoxins must take place before the derivatised aflatoxins enter the fluorescence detector. -
Page 7: Kobra® Cell Contents
Incinerate waste if • 1 x KOBRA® CELL Installation Pack regulations permit. (Product Code: K03)* • Guard column: Inertsil ODS-3, 5 µm, 4 mm x 10 mm or equivalent * Available from R-Biopharm. Please contact your local distributor for further information. -
Page 8: Assembly Of The Kobra® Cell
R-Biopharm Rhone Ltd Assembly of the KOBRA CELL ® Components 1. 6 locating screws 2. White plastic housing with red terminal (top) Contact 3. PTFE spacer with reaction Area for area: 0.1 mm Electrodes (K09) 4. Platinum electrode (K05) 5. PTFE spacer: 0.25 mm (K07) 6. -
Page 9: Precautions When Assembling
Precautions When Assembling • To reassemble the KOBRA® CELL it is important to • Never use metal nuts or ferrules directly with the tighten the locating screws at opposite corners KOBRA® CELL. before tightening the middle screws. This will • Use a guillotine tubing cutter to prevent distortion ensure that the KOBRA®... -
Page 10: Kobra® Cell Installation
R-Biopharm Rhone Ltd KOBRA CELL Installation ® Power Pack Notes Power Pack Technical Specification It should be noted that the KOBRA® CELL power pack has been improved and as such is of a slightly Power Supply Primary 110 - 240 V different design. -
Page 11: Installation Guide
Installation Guide Detector Inlet Detector Outlet Column Detector Reaction Coil Red Terminal Working Electrode Inlet Outlet (platinum) Spacer Membrane Spacer Counter Electrode (Stainless Steel) Inlet Outlet Black Terminal Waste Membrane: Reichelt Thomapor Anion. Capillary connections: Valco 1/16 inch (do not use metal nuts or ferrules because these can damage the electrochemical properties of the cell). -
Page 12: Installation Instructions
R-Biopharm Rhone Ltd Installation Instructions Caution: Do not over tighten. Cell connections 6. Connect the detector outlet to the KOBRA® CELL should be made with plastic ferrules, not metal. inlet using plastic ferrules (hand tightened) and PEEK tubing. 1. Unpack the KOBRA® CELL and check that all of the components are present. -
Page 13: Precautions To Take Before Running The Kobra® Cell
Precautions to Take Before Operating the KOBRA® CELL Running the KOBRA CELL ® The KOBRA® CELL power pack is turned on by moving the switch to the ‘On’ position. To shut • This device has only to be used with the supplied down the device move the switch to the ‘Off’... -
Page 14: Hplc Conditions
R-Biopharm Rhone Ltd HPLC Conditions HPLC Conditions Derivatisation KOBRA® CELL at 100 µA setting Guard Cartridge Inertsil ODS-3 5 µm, 4 mm x 10 mm or (Hichrom) equivalent Analytical Column Inertsil ODS-3V 5 µm, 4.6 mm x 150 mm (Hichrom) or equivalent... -
Page 15: Kobra® Cell Maintenance
KOBRA CELL Maintenance ® Storage To fill the KOBRA® CELL with water: • The KOBRA® CELL should be switched off and 1. Fill a syringe with 5 - 10 ml of water and attach disconnected for washing and storage (Fig. 5). the tip of the syringe to a pipette tip. -
Page 16: Daily Cleaning
R-Biopharm Rhone Ltd Daily Cleaning The HPLC system can be left running overnight at 6. Manually flush the KOBRA® CELL through with a reduced flow rate (e.g. 0.1 ml/min) and with the distilled water several times using a syringe. fluorescence detector and KOBRA® CELL power source turned off, if necessary. -
Page 17: Monitoring Performance
Monitoring Performance It is necessary to regularly monitor the performance 1. Using a screw driver take off the white plastic of the KOBRA® CELL in order to detect any housing of the KOBRA® CELL by removing the deterioration in the membrane contained within. 6 locating screws. -
Page 18: Troubleshooting
R-Biopharm Rhone Ltd Troubleshooting No Peaks Observed • If no peaks are observed on the HPLC • Check the PEEK tubing leading to and from the chromatogram it is probably not the KOBRA® CELL KOBRA® CELL is not blocked or distorted. Replace if that is at fault (Fig. -
Page 19: Reduced / No Aflatoxin B1 And G1 Peaks Observed
Reduced / No Aflatoxin B1 and G1 Peaks Observed • Check the KOBRA® CELL is connected correctly to • Check there is power in the power pack. Check the HPLC (Fig 6). that the power pack has been turned on. •... -
Page 20: Abnormal Peaks Observed
R-Biopharm Rhone Ltd Abnormal Peaks Observed • If abnormal peaks are observed on the HPLC • Check the internal components of the chromatogram it is not normally the KOBRA® CELL KOBRA® CELL have been inserted in the correct that is at fault (Fig. -
Page 21: Error Light Observed On Power Pack
Leakage from KOBRA® CELL Storage Plug Broken Off or Lodged in KOBRA CELL ® • The locating screws on the KOBRA® CELL have not been tightened correctly. Please refer to 3.2, Precautions When Assembling. • Return to R-Biopharm Rhône for repair. -
Page 22: General Information
General Information Quality Warranty RBR products are developed, manufactured, R-Biopharm Rhône Ltd makes no warranty of any tested and dispatched under an ISO 9001 and ISO kind, express or implied, except that all products 13485 registered Quality Management System, made by R-Biopharm Rhône Ltd are made with guaranteeing a consistent product, which always materials of suitable quality. - Page 24 R-Biopharm Rhône Ltd R-Biopharm Rhône Ltd Block 10 Todd Campus West of Scotland Science Park Acre Road, Glasgow G20 0XA www.r-biopharm.com R-Biopharm – for the safety of your analysis.