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R-Biopharm Rhone Ltd
Troubleshooting
7
No Peaks Observed
7.1
•
If no peaks are observed on the HPLC
chromatogram it is probably not the KOBRA® CELL
that is at
fault (Fig. 8).
There may be a problem
with a component of the HPLC system or with the
standards used.
•
Check that the bulb in the lamp does not need
replaced. It should be replaced every 1,000 - 2,000
hours depending on the detector.
•
Check there is not a problem with the detector.
•
Check the standard has been prepared correctly,
or the standard has not deteriorated. Standards
should be prepared fresh every day.
Fig 8. No Peaks Obtained in Chromatogram
•
Check the PEEK
tubing leading to and from the
TM
KOBRA® CELL is not blocked or distorted. Replace if
required.
•
Check the internal components of the
KOBRA® CELL have been inserted in the correct
order, particularly if no peaks are observed
following the replacement of one of the
components (Fig. 1).
18
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