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R-Biopharm Rhone Ltd
Installation Instructions
4.4
Caution: Do not over tighten. Cell connections
should be made with plastic ferrules, not metal.
1. Unpack the KOBRA® CELL and check that all of
the components are present.
2. Add 119 mg of potassium bromide and 350 µl of
4 M nitric acid to 1 l of mobile phase.
3. Immerse the tubing from the HPLC pump into
the mobile phase.
4. Disconnect the storage plugs and capillary
tubing from the KOBRA® CELL. Connect the
HPLC column discharge to the KOBRA® CELL
inlet using plastic ferrules (hand tightened) and
a length of PEEK
tubing supplied with the kit.
TM
5. Connect the KOBRA® CELL outlet using ferrules
(hand tightened) and a length of PEEK
to the detector inlet according to the table
below.
Caution: Use a guillotine tubing cutter to
prevent distortion of the diameter of the tubing.
Determination of Reaction Coil / PEEK
Flow Rate
0.5 ml/min
Internal
0.2 mm
diameter
0.25 mm
of reaction
0.4 mm
coil /
PEEK
TM
0.5 mm
tubing (id)
0.6 mm
0.8 mm
1.0 mm
tubing
TM
0.6 ml/min
106.1 cm
127.3 cm
67.9 cm
81.5 cm
26.5 cm
31.8 cm
17.0 cm
20.4 cm
11.8 cm
14.1 cm
6.6 cm
8.0 cm
4.2 cm
5.1 cm
6. Connect the detector outlet to the KOBRA® CELL
inlet using plastic ferrules (hand tightened) and
PEEK
tubing.
TM
7. Connect the KOBRA® CELL outlet to waste using
plastic ferrules (hand tightened) and PEEK
tubing.
8. Switch on the HPLC pump and flush mobile
phase through the system for approximately
5 minutes.
9. Connect the KOBRA® CELL power pack to the
cell, red lead to red terminal and black lead to
black terminal.
Caution: Do not switch on without mobile
phase flowing through the KOBRA® CELL or the
membrane will be damaged.
10. After the HPLC has been allowed to stabilise
(approximately 30 minutes) the KOBRA® CELL is
ready to use.
Tubing Length
TM
0.7 ml/min
0.8 ml/min
148.5 cm
169.8 cm
95.1 cm
108.6 cm
37.1 cm
42.4 cm
23.8 cm
27.2 cm
16.5 cm
18.9 cm
9.3 cm
10.6 cm
5.9 cm
6.8 cm
12
TM
0.9 ml/min
1.0 ml/min
191.0 cm
212.2 cm
122.2 cm
135.8 cm
47.7 cm
53.1 cm
30.6 cm
34.0 cm
21.2 cm
23.6 cm
11.9 cm
13.3 cm
7.6 cm
8.5 cm
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