Derivatisation Reaction - R-Biopharm KOBRA CELL Instruction Manual

R-Biopharm Rhone Ltd

Derivatisation Reaction

1.2
The aflatoxins and the mobile phase enter the
KOBRA® CELL and the electrochemical reaction
occurs generating the reactive form of bromine.
The reaction between the reactive bromine and the
aflatoxins must take place before the derivatised
aflatoxins enter the fluorescence detector. Hence
the length of the reaction coil is critical. A minimum
reaction time of 4 seconds is required.
H
Aflatoxin B1
H
H
H
Aflatoxin G1
6
H
+ Br
2
H
H
+ Br
2
H