Related Manuals for MshOt MF53-N
Summary of Contents for MshOt MF53-N
- Page 1 Guangzhou Micro-shot Technology Co., Ltd MF53-N Inverted fluorescence microscope User manual Website：www.m-shot.com Tel:0086-20-38262481 Email: sales@mshot.com...
- Page 2 Inverted fluorescence microscope MF53-N...
- Page 3 Thank you for buying our product! This unit is a precision optical instrument. Though with high safety design, wrong usage and overlook of this manual can do harm to you and your property. Thus, to ensure the life of this unit and maintain it properly, please read this manual carefully before operating.
- Page 5 This unit employs a coarse/fine coaxial focusing mechanism. Do not turn the left/right coarse/fine focusing knob in the opposite direction. When the objectives lifting device reaches the limit of motion, do not continue to turn the coarse focusing knob, otherwise the focusing mechanism may be damaged. Storage place Caution This unit is a precision optical instrument, and improper operation or storage may cause damage or...
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Page 7: Table Of Contents
Content 1. Components……………………………………………………………………………..1 2. Main observation steps…………………………………………………………..…..3 ....................3 2-1、Bright field observation .....................5 2-2、Phase contrat observation ....................6 2-3、Fluorescence observation 3. Installation and debugging of microscope………………………..………………..8 4. Maintanence………...…………………………………………………………………12 5. Specification……………………………………………………………..….…………14 6. Troubleshooting……………………………………………………………………… 错 误！未定义书签。... -
Page 9: Components
1、Components LED light source (bright field) Phase contrast plate Condenser Stage Eyepiece Splitter lever Stage control wheel Focusing wheel Coarse and fine focusing system... - Page 10 Light barrier Nosepiece Broad spectrum LED lamp house Fluorescence module Camera port Microscope body Coarse and fine focusing wheel Broad spectrum LED power control box（fluorescence） LED light control box（bright field）...
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Page 11: Main Observation Steps
2、Main observation steps Bright field observation ❶Turn on the bright field power supply / light intensity adjustment switch knob (1), and the sound of "Ta" ① indicates that it is on. Adjust the light intensity: rotate the knob on the control box of bright field power supply to adjust the light source from "0"... - Page 12 ❺Adjust the aperture diaphragm adjusting rod ⑤ to change the intensity of light passing through the condenser. The position of the aperture should correspond to the magnification of the objective lens. For example, when observing under the 10x objective lens, it is recommended to adjust the adjusting rod of the aperture to one third to the right and one third to the left, so that the light passing through is enough to ensure the best observation effect.
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Page 13: Phase Contrat Observation
Phase contrast observation ❾On the basis of bright field observation, rotate the objective nosepiece ④ to turn the 10x phase contrast objective into the optical path. The shell of phase contrast objective has the word "pH" ④ ❿Push the phase insert plate ⑤ into the slot of the phase insert plate (refer to the installation of the phase insert plate in the installation procedure of the microscope), so that the phase difference ring of the corresponding multiple of the... -
Page 14: Fluorescence Observation
Fluorescence observation ⓭Push the condenser baffle plate into the light path. In fluorescence observation, the baffle of the condenser can reduce the stray light reflected by the condenser into the light path and affect the fluorescence observation effect ⑥ ⓮Gently press the word "play" on the left side of the fluorescent power control box to turn on the fluorescent light source, and adjust the fluorescent light intensity knob to the appropriate brightness. - Page 15 Fluorescence Mark EX wavelength（nm） DM wavelength （nm） EM wavelength（nm） 330-380 420LP Blue 460‒490 510LP Green 510‒550 590LP Neutral ⓱Adjust the focusing hand wheel ⑧ to focus the sample. ⑧ The following procedure is applicable to all observation methods ⓲After adjustment, the coarse focusing limiting hand wheel adjusting ring can prevent the object from colliding with the sample.
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Page 16: Installation And Debugging Of Microscope
⓴Adjust the pupil distance: when observing through the eyepiece, adjust the binocular until the left and right fields of view are completely consistent. The indicator dot "." indicates the pupil spacing. Write down your pupil spacing scale for next use Adjust the diopter: after focusing the right lens tube clearly, observe the left lens tube again. - Page 17 ❸ ❸Place the stage above the bracket gasket (keep the screw holes aligned), and screw the hexagon screw into the screw hole to fix the stage. ❹Place the drip plate over the stage. ❹ ❺ ❺Open the dust cover of the eyepiece tube and clip the two eyepieces into the tube respectively.
- Page 18 ❼Insert the phase contrast plate into the slot above the ❼ condenser. ❽Remove one eyepiece and insert the centering telescope into the eyepiece tube. The 10x objective is turned into the optical path, and the phase insert plate is pushed into the optical path corresponding to the 10x hole position.
- Page 19 ❿Clip the fluorescent baffle into the screw in front of ❿ the microscope bracket. The fluorescent baffle can protect the eyes of the observe. ⓫Clip the light box interface of wide spectrum LED light source into the light box bayonet behind the microscope bracket, and tighten the two screws on the side to fix ⓫...
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Page 20: Maintanence
⓮Clip the fluorescent module into the module base ⓮ from the left side of the microscope, and slowly push it along the base track to make the module clip into the template base plate. ⓯Use a hexagon key to tighten the fixing screw on the bottom plate of the module on the right side of the microscope to fix the fluorescent module on the bottom plate to avoid shaking. - Page 21 in a little alcohol ether 1:4 mixture; Alcohol, ether, etc. are highly combustible, Keep away from fire 。 Be careful not to catch fire when switching the power supply; Avoid using organic solution such as alcohol, ether or their mixture to clean the metal paint surface and electroplating surface.
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Page 22: Specification
5、Specification Binocular observation tube, 45°inclined, pupil distance adjust range 50-75mm, diopter adjustable Observation 0%/100% light pass for digital camera or eyepiece WF10X/23 plan eyepiece, high eyepoint Eyepieces Centering telescope Infinite long working distance plan achromatic: LWDPlan 4X/0.1 Infinite long working distance plan achromatic: Plan10X/0.25, working distance 4.1mm Infinite long working distance plan achromatic: Plan 40X/0.58, working distance 2.5mm... -
Page 23: Troubleshooting
Blue (B) EX:460-490nm,DM:500nm,EM:510nmLP Green (G) EX:510-550m,DM:570nm,EM:590nmLP Long working distance condenser 72mm, NA 0.30 with triple phase contrast Condenser slider 9W warm LED brightness contentiously adjustment Transmitted illu mination system LED rotary brightness control knob Camera adapter 0.75X C-mount adapter 6、Troubleshooting 11-1 Optical part Problems Reason... - Page 24 No power supply or poor Check the wire connection contact The bulb does not light when the switch is on The light bulb is broken Replace The voltage is too low Increase the voltage Voltage problem Check voltage stability Flashing or unstable light The bulb is not properly bulb Check and firmly plug it in...
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