Related Manuals for MshOt MF43-N
Summary of Contents for MshOt MF43-N
- Page 1 Guangzhou Micro-shot Technology Co., Ltd MF43-N Research grade fluorescence microscope User Manual Website：www.m-shot.com Tel:0086-20-38262481 Email: sales@mshot.com...
- Page 2 Research grade fluorescence microscope MF43-N...
- Page 3 Thank you for buying our product! This unit is a precision optical instrument. Though with high safety design, wrong usage and overlook of this manual can do harm to you and your property. Thus, to ensure the life of this unit and maintain it properly, please read this manual carefully before operating.
- Page 4 Coarse/fine focusing knobs Warning! This unit employs a coarse/fine coaxial focusing mechanism. Do not turn the left/right coarse/fine focusing knob in the opposite direction. When the objectives lifting device reaches the limit of motion, do not continue to turn the coarse focusing knob, otherwise the focusing mechanism may be damaged. Storage place Caution This unit is a precision optical instrument, and improper operation or storage may cause damage or...
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Page 5: Table Of Contents
Content Ⅰ. Components........................1 Ⅱ. Installation and operation....................2 1. Baisc operation................................3 2. Microscope operation..............................4 3. Adjust alignment................................5 4. Adjustable aperture diaphragm..........................6 5. Focusing system................................. 7 6. Stage.................................... 8 7. Adjust the tension of the x-axis and y-axis knobs....................8 8.Adjust the alignment of fluorescent light path.....................11 Ⅲ. -
Page 7: Ⅰ.components
Ⅰ.Components Trinocular tube Fluorescence illuminator lamp house Eyepiece Fluorescence filter wheel Host Fluorescence light barrier plate X/Y stage hand wheel Field diaphragm... - Page 8 Camera adapter （need to buy separately） Fluorescence illuminator Objective Bright field illuminator power button Stage Light intensity rotate knob Focusing wheel...
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Page 9: Ⅱ.installation And Operation
Ⅱ.Installation and operation 2-1、Basic operation 1. Turn on the power supply: turn on the power supply and turn the main switch on the side of the microscope to the "-" state. 2. Adjust the light intensity: turn the knob to adjust the lighting intensity. -
Page 10: Microscope Operation
to reset the best brightness, turn off LIM switch ① (when LIM indicator light ② goes out), turn the objective lens that needs to reset the best brightness into the optical path, turn off Lim switch, and then repeat the above steps 3 and Rotating the focusing knob to lower the stage. -
Page 11: Adjust Alignment
① to focus the sample. Do not adjust the coarse and fine focusing knob during focusing system. Use the left eye to observe from the left lens, turn the left diopter adjusting ring ② to focus the sample. Use eyepiece rubber clap When wearing glasses, put the goggles in the normal folded position to prevent eye contact and scratching. -
Page 12: Adjustable Aperture Diaphragm
4. Turn the height of the condenser and adjust the knob of the condenser to raise the condenser to the highest level. 5. Rotate the field of view diaphragm adjusting ring along the direction of the arrow to reduce the diaphragm. 6. -
Page 13: Focusing System
2. Remove the eyepiece and observe from the lens barrel. The aperture image can be seen, as shown in Figure 2 above. 2-5Focusing system Coarse focus knob: move the stage up and down to focus Fine focus knob (right side of frame): fine adjustment of focusing position Fine focus knob (left side of bracket) to fine adjust the focus position Coarse focus limiting device: set the upper limit of stage position (to avoid collision) -
Page 14: Stage
2-6 Stage ①Turn the coarse focusing knob to lower the stage. ②Open the spring finger ② on the slide holder, slide one or two sample slides from the front into the stage. ③Push the slide in as far as you can, and then gently release the trigger The slide size should be 26 ×... -
Page 15: Ⅲ. Installations Steps
Adjust the height of the stage 1、Lower the stage to the lowest level, and then remove the stage from the microscope. 2、Using a hexagon screw driver, loosen the fixed screw ① of the stage bracket to remove the stage bracket. 3、Turn the coarse focus knob to raise the focus slide plate ③... -
Page 16: Install Objective Nosepiece
2、Use the hexagon key to tighten the set screw ②. 3、 Insert the plug ④ on the lamp chamber into the socket of microscope ⑤. 3-2Install the objective nosepiece 1、Turn the coarse focusing knob to lower the stage. 2、Slightly loosen the fixing screw ① of the objective turntable. -
Page 17: Adjust The Alignment Of Fluorescent Light Path
3-4 Install the condenser 1、Turn the coarse focusing knob ① to raise the stage to the highest position. 2、Turn the condenser height adjusting knob ② to minimize the condenser frame. 3、Completely loosen the fixing screw ③ of the condenser. 4、Hold the condenser with the numerical aperture number facing forward. Carefully push the condenser into the innermost part along the dovetail slot. -
Page 18: Install Fluorescence Attachment
3-7 Install the fluorescent module Clip the dovetail interface of the fluorescence module into the dovetail slot of the microscope host, ① and tighten the right inner hexagon screw ①. 3-8 Installation of fluorescent light ① Clip the mercury lamp box into the interface behind the fluorescent module, and tighten the two fixing screws ①... -
Page 19: Install Eyepiece Tube
power line. 3-10 Install the eyepiece tube ① Clip the dovetail connector of the three lens barrel into the dovetail slot above the fluorescent module, and tighten the right inner hexagon screw ①. ① 3-11 Installing eyepiece Gently insert the two eyepieces into the eyepiece barrel until the end. -
Page 20: Install The Power Cord
3-13 Install the power cord 1、Firmly insert the power cord plug ① into the input socket ② of the adapter... -
Page 21: Ⅳ、Broad Spectrum Led Light Source
Ⅳ、Broad spectrum LED light source Broad spectrum LED light sour lamp house Power control box This light source is an optional product, providing three bands of LED light sources, corresponding to UV 360-380nm, B 430-465nm and G 505-545nm respectively. 4-1 Basic operations 1、Turn off the lower light source used in the bright field observation, and insert the power plug of the power control box into the power... - Page 22 2、Turn on the switch of the power control box, and the power indicator is on. The condenser of the microscope is lowered and the aperture is reduced. 3、The knob ① is used to adjust the brightness of ② ① b-band and G-band light source, and the knob ② is used to adjust the brightness of UV band light source.
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Page 23: Ⅴ、Troubleshooting Guide
accessory of the microscope, clamp the ① wide spectrum LED light source into the connector, and then tighten the screw. 4-3 Adjusting the condenser ① Adjust the condenser in front of the light box to make the light source converge. Ⅴ、Troubleshooting guidance Problem Lighting... - Page 24 The split rod is located at Move the splitter lever to position The condenser is not Re centering the condenser properly aligned The condenser is too low Adjust the height of condenser Mercury lamp power is Turn on the switch on the mercury not on lamp power control box The light path of mercury...
- Page 25 Make sure that the objective The objective nosepiece nosepiece is in the position of the is not in place click sound card The objective nosepiece Push the sliding dovetail The field of is not installed correctly connector all the way to the inside view is not bright, even The condenser is not...
- Page 26 position The image is The objective nosepiece Push the dovetail joint all the way not obvious/ is not installed correctly to the inside The lens of the objective Clean the objective lens Poor contrast/ is dirty For oil mirrors, no Use immersion oil The details immersion is used...
- Page 27 one side click sound card Incorrect installation of Re install the stage stage Incorrect installation of Push the dovetail joint all the way objective nosepiece to the inside The condenser is out of Re-centering the condenser alignment The thickness of the Replace the sample or prepare the sample is not uniform sample again...
- Page 28 coarse collimation knob Image cannot be focused / the stage Common/ Too loose adjustment of slides Tighten the loose device of coarse fluorescen coarse collimation knob automatically collimation knob tightening device / the coarse and fine focus knob slides The coarse Common/ The coarse collimation focusing can...
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Page 29: Ⅵ、Technical Specification
eye is The diopter was not Correct adjustment of diopter different from adjusted properly that of the The eyepiece used in the other left and right eyes is Use two identical eyepieces different When viewing from the eyepiece, Not suitable for look at the whole field of view microscopic observation before focusing on the sample... - Page 30 ★ Clean the objective lens regularly. The objective lens is very sensitive to dust. ★ During the operation, low power (such as 4x / 10x) objective lens is used to search and focus the sample, and then other power objective lens is used for observation as needed.
- Page 31 Warm white LED lamp, brightness is continuously adjustable illumination Abbe condenser, N.A. 1.1 system Power line According to user’s country electrical requirement Screw driver M3.0 hexagon screw driver Accessories Immersion oil Dust cover MSHOT special dust cover Optional adapters 1X/0.5X/0.75X C-mount adapter...
- Page 32 Optional accessories Model Specification Semi-fluorescence objective Plan Fluor 20X/0.5 working distance：2mm UPLFLN4X/0.13 working distance：17mm Olympus semi plan UPLFLN10X2/0.3 working distance：10mm fluorescence objective UPLFLN20X/0.5 working distance：2.1mm （production time is not UPLFLN40X/0.75 working distance：0.51mm stable） UPLFLN100XO2/1.3 working distance：0.2mm M-PCD-BX PLCN10X/0.25PH working distance：10.6mm Phase contrast components PLCN20X/0.4PH working distance：1.2mm PLCN40X/0.65PH working distance：0.6mm...
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