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Oxford Instruments ANDOR Mosaic3 Hardware Manual

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Mosaic3
Version 3.5 revised 8 May 2019
Hardware Guide
(covers Mosaic 3 and Infinity USB 3.0 models)
andor.com
© Andor Technology Ltd. 2019

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Summary of Contents for Oxford Instruments ANDOR Mosaic3

  • Page 1 Mosaic3 Version 3.5 revised 8 May 2019 Hardware Guide (covers Mosaic 3 and Infinity USB 3.0 models) andor.com © Andor Technology Ltd. 2019...

  • Page 2: Table Of Contents

    Mosaic3 TABLE OF CONTENTS SECTION 1: INTRODUCTION ............. 25 TECHNICAL SUPPORT ..................26 DISCLAIMER ....................27 COPYRIGHT AND PROTECTIVE NOTICES ............. 27 TRADEMARKS AND PATENT INFORMATION ..........27 SUPPLIED COMPONENTS ................28 OPTIONAL COMPONENTS ................28 SECTION 2: PRODUCT OVERVIEW ........... 29 GENERAL ......................
  • Page 3 Mosaic3 2.6.3 S ..........36 yStem igHt patH nverted iCroSCope 2.6.4 n ...................... 36 oteS SYSTEM CONTROL ..................37 SOFTWARE REQUIREMENTS ................37 INTERLOCK SAFETY SYSTEM ................ 38 SECTION 3: HARDWARE DESCRIPTION ........39 MOSAIC DMD HEAD ..................39 3.1.1 p .....................
  • Page 4 Mosaic3 SECTION 4: INSTALLATION ............41 LOCATION AND MOUNTING ................41 VENTILATION ....................41 ASSEMBLY ......................41 POWER CONNECTION AND PROTECTIVE EARTHING ........41 MOSAIC CABLING .................... 42 MOSAIC OPTICAL FIBRE ................. 42 SOFTWARE INSTALLATION ................42 REMOTE INTERLOCK ..................42 SYSTEM COMPONENTS AND ACCESSORIES ..........
  • Page 5 Mosaic3 5.4.7 o .................... 47 verLap 5.4.8 e ................... 47 xternaL riggering 5.4.9 e ................ 47 xternaL rame ddreSSing 5.4.10 d (dmd a ) ........... 47 eviCe ynCHronization Ctive UtpUt 5.4.11 B ..............47 pLitter peration 5.4.12 L ) ........ 48 oUSe iLterS not avaiLaBLe on...
  • Page 6 Mosaic3 SECTION 8: TECHNICAL SPECIFICATIONS ......57 AC/DC POWER SUPPLY REQUIREMENTS ............ 57 POWER CONSUMPTION .................. 57 ELECTRICAL SPECIFICATIONS (I/O) ............... 57 OPERATING CONDITIONS ................58 STORAGE AND TRANSPORTATION ..............58 PERFORMANCE SPECIFICATIONS ..............58 LASER SOURCES ..................... 58 APPENDIX A: MECHANICAL DRAWINGS ......... 59 APPENDIX B: GLOSSARY ............
  • Page 7 Mosaic3 Revision History Version Released Description 24 Sep 2010 Initial Release 15 Feb 2012 Conversion into InDesign format 09 Jul 2013 Minor updates (All sections) 10 Oct 2013 Updated Laser Safety Information. Updated presentation (All sections) Added PDF Functionality to improve navigation 19 Dec 2013 Fully revised and updated information to match latest Mosaic 3 model features and functionality (all sections)
  • Page 8 Mosaic3 Safety and Warning Information PLEASE READ THIS INFORMATION FIRST CAUTION – USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE. If the equipment is used in a manner not specified by Andor, the protection provided by the equipment may be impaired.
  • Page 9 Mosaic3 19. This product has been designed and tested to perform successfully in a normal (basic) electromagnetic environment, e.g. a typical life science test laboratory, as per the EU EMC Directive. It is not designed to operate in a harsh electromagnetic environment, e.g. close to the following equipment: EMI/RFI generators, electrostatic field generators, electromagnetic or radioactive devices, plasma sources, arc welders, x-ray instruments, intense pulsed sources, or other similar sources of high energy fields whose emissions are not within the normal range expected under the EU EMC Directive.
  • Page 10 Mosaic3 aser afety peciaL arnings CAUTION – USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE This product includes lasers or is designed to be upgraded with lasers, so you must be aware of the hazards associated with the use of the powerful laser radiation that can be emitted by this product.
  • Page 11 Mosaic3 ecommended esponsibiLities of a aser afety fficer These include, but are not restricted to, the following. However, national and industry guidelines should also be referred to, e.g. IEC 60825-14 Section 3.3. Ensure all personnel requiring access to the product are fully trained in both using the product and the general use of Class 3B and Class 4 lasers Ensure users are familiar with the hazardous properties of lasers, especially that laser safety hazards differ from those of normal light sources as they are highly-concentrated, very low divergence beams of radiation...
  • Page 12 Mosaic3 uideLines for peration of aser roducts Read the safety instructions supplied with all equipment in the system Never look into a laser beam either directly or indirectly Do not attempt to disassemble the unit housing the lasers or any part of the system. If there is a problem please contact Andor directly (see Section 1.1, “Help and Technical Support”) Restrict and control access to the area(s) where laser(s) are in use to those persons who are trained in the dangers of lasers and trained on the safety precautions to be observed when working with lasers...
  • Page 13 Mosaic3 aser afety and roduct ompLiance abeLs eneraL ymboLs WARNING: Laser radiation hazard This product has been tested to the requirements of CAN/CSA-C22.2 No. 61010-1, 2nd Edition, including Amendment 1, or a later version of the same standard incorporating the same level of testing requirements.
  • Page 14 Mosaic3 Lass This laser radiation can damage your eye, but will not normally damage your skin, and will be identified as follows: WARNING – CLASS 3B LASER RADIATION – AVOID EXPOSURE TO BEAM These are defined by IEC 60825-1 Annex C.2 as “laser products that are normally hazardous when intrabeam ocular exposure occurs (i.e.
  • Page 15 Mosaic3 xpLanatory abeLs With roduct Lassification adiation utput and tandards nformation Each Mosaic laser product will have one of these labels located on the microscope as close as reasonably practicable to the laser aperture at the stage. In some older models, the same information will be located on the Fibre Collimator, where the optical fibre enters the main product.
  • Page 16 Mosaic3 aser perture abeLs A number of these labels are located at various points where laser is emitted from an aperture either deliberately at the microscope objective or potentially as a result of disassembly during installation or servicing. nternationaL aser perture abeLs icroscope...
  • Page 17 Mosaic3 2: c otentiaL aser perture abeL overed ncovered daLaser utput 3: h otentiaL aser perture abeL omogeniser pticaL ibre utput Version 3.5 rev 8 May 2019...
  • Page 18 Mosaic3 4: c otentiaL aser perture abeL overed ncovered ibre oLLimator utput This is the part that through which laser radiation from the optical fibre enters the main Mosaic unit. Version 3.5 rev 8 May 2019...
  • Page 19 Mosaic3 5: s otentiaL aser perture abeL tandard daptor utput The standard, or non-infinity, versions of Mosaic connect to the microscope via its epi-port and have a potential laser aperture if installers/ service personnel disconnect the system at this point. Version 3.5 rev 8 May 2019...
  • Page 20 Mosaic3 6: i otentiaL aser perture abeL nfinity icroscope daptor oWer ransmission There is a potential laser aperture where the Infinity versions of Mosaic connect to the microscope, if installers/ service personnel disconnect the system at this point. Version 3.5 rev 8 May 2019...
  • Page 21 Mosaic3 ccess aneLs Access panels are defined in IEC 60825-1 as “part of the protective housing which provides access to laser radiation when removed or displaced.” In most cases, you should have no reason to open these and only service personnel will need to gain access, but the laser standards direct us to warn you of access using the following labels.
  • Page 22 Mosaic3 2: o ccess aneL abeL pticaL ight nput Lock nterLock over There is a tool-accessible panel to allow access to an interlock for installation/service personnel as follows: Version 3.5 rev 8 May 2019...
  • Page 23 Mosaic3 3: o ccess aneL abeL pticaL ight nput Lock econdary Lanking Late 4: o ccess aneL abeL pticaL ocus djustment Version 3.5 rev 8 May 2019...
  • Page 24 Mosaic3 5: i ccess aneL abeL nfinity icroscope daptor pper mission There is a potential access point where the Infinity versions of Mosaic connect to the microscope, if installers/ service personnel disconnect the system at this point. cdrh c ertification abeL This self-certifies that we comply with the U.S.

  • Page 25: Section 1: Introduction

    Mosaic3 INTRODUCTION SECTION 1: INTRODUCTION Built around MEMS Digital Micromirror Devices (DMD), the Mosaic family (Mosaic 3 and Mosaic Infinity) is a patented instrument for targeted illumination (photostimulation) on microscopy systems. High speed frame switching (5,000 fps) makes the Mosaic suitable for many dynamic applications including optogenetics, bleaching, uncaging, photo-switching and constrained illumination.

  • Page 26: Technical Support

    Mosaic3 INTRODUCTION 1.1 t echnicaL upport If you have any questions regarding the use of this equipment, please contact the representative* from whom your system was purchased, or: Europe Andor Technology Ltd. Andor Technology 7 Millennium Way 300 Baker Avenue Springvale Business Park Suite # 150 Belfast...

  • Page 27: Disclaimer

    Andor and the Andor logo are trademarks of Andor Technology Ltd. Andor is an Oxford Instruments company. All other marks are property of their owners. The system and various components in the system are the subject of the following US patents: 6,885,492 6,072,892 and 7,034,983 other patents pending.

  • Page 28: Supplied Components

    Mosaic3 INTRODUCTION 1.5 s uppLied omponents Refer to the packing list supplied with your system and inspect against the components specified at time of ordering. 1.6 o ptionaL omponents There are a range of optional components available for the Mosaic 3. Please contact your nearest Andor Sales Representative for further information on these and other system components available.

  • Page 29: Section 2: Product Overview

    Mosaic3 PRODUCT OVERVIEW SECTION 2: PRODUCT OVERVIEW 2.1 g eneraL The Mosaic is highly configurable so the exact configuration of your product will depend on the options specified at the time of purchase. The main components are described in the following sections: Microscope interface Figure 2: Mosaic Components (Mosaic 3 shown) This houses all of the control electronics and the DMD Array and provides all of the electrical and control...

  • Page 30: Mosaic Infinity Configuration

    Mosaic3 PRODUCT OVERVIEW 2.2 m osaic nfinity onfiguration The Mosaic Infinity version enables the mosaic to be integrated into an upright microscopy set-up. The features and functionality of the infinity version are the same as the Mosaic 3 (Section 2.1 ) apart from as detailed in the following sections.

  • Page 31: Overview Of Dmd Array Technology

    Mosaic3 PRODUCT OVERVIEW 2.3 o dmd a vervieW of rray echnoLogy The core of Mosaic is the Digital Micro Mirror Device (DMD), a high speed and highly efficient semiconductor-based “light switch” array (800 x 600), of hinge-mounted, addressable, tiltable, microscopic mirrors. When a DMD chip is co- ordinated with a digital video or graphic signal, a light source, and beam delivery optics, its mirrors reflect a digital image of the illumination mask onto the sample.

  • Page 32: System Components

    Mosaic3 PRODUCT OVERVIEW 2.4 system components 2.4.1 usb 405 aser ontroLLer If the Mosaic is supplied with a 405 nm Laser, then this interface allows manual power control of the 405 nm Diode Laser or selection of USB control to allow the control software interface to set the laser’s output power level. 2.4.2 L aser omogenizer...

  • Page 33: System Overview

    Mosaic3 PRODUCT OVERVIEW 2.5 s ystem vervieW An example of a typical system configuration is shown below: USB etc. Computer Camera Light USB 3.0 Source RS232 USB 3.0 cable Camera Port Lamp Shutter PS-12 +12V Eyepieces house Controller tube Microscope Mosaic 3 Optical Safety...

  • Page 34: 2.6 Optical Layout

    Mosaic3 PRODUCT OVERVIEW 2.6 Optical Layout 2.6.1 m osaic nternaL ight aths There are two main variants of the Mosaic device. Standard (Single Input Block). Duet (Dual Input Blocks). The Internal Light Paths, in this example for Mosaic 3, are shown below: Figure 7: Internal light path of Mosaic 3 Standard version Figure 8: Internal light path of Mosaic 3 Duet version Version 3.5 rev 8 May 2019...

  • Page 35: S Ystem L Ight Path : U Pright M Icroscope

    Mosaic3 PRODUCT OVERVIEW 2.6.2 s ystem ight path pright icroscope An example for an upright microscope is shown below: Figure 9: Upright Microscope light path (Mosaic Infinity) Version 3.5 rev 8 May 2019...

  • Page 36: S Ystem L Ight Path - I Nverted M Icroscope

    Mosaic3 PRODUCT OVERVIEW 2.6.3 s ystem ight path nverted icroscope An example for an inverted microscope system is shown below: Fluorescent Sample Laser Excitation Light and or Widefield Light Mosaic 3 Eyepiece Interlock Sensor (Activated) Camera Eyepiece’s Light Path Blocked (Prism is in Camera Port Position) Fluorescent Emitted Light...

  • Page 37: System Control

    Mosaic3 PRODUCT OVERVIEW 2.7 s ystem ontroL The Mosaic must be interfaced to a workstation to allow control. The workstation communicates with the Mosaic via a USB 3.0 connection. Control software is required to interface with and control the device*. *(an SDK is available for system integrators or advanced users, please contact your Andor Sales Representative to apply for access).

  • Page 38: Interlock Safety System

    Mosaic3 PRODUCT OVERVIEW 2.9 i nterLock afety ystem If the Mosaic is supplied with a laser source it will incorporate an interlock safety system to protect the users of the system. The Mosaic contains no user serviceable parts- do not attempt to disassemble the system or the enclosure. Disassembly is only to be performed by trained and certified Andor Service Personnel.

  • Page 39: Section 3: Hardware Description

    Mosaic3 HARDWARE DESCRIPTION SECTION 3: HARDWARE DESCRIPTION 3.1 m dmd h osaic This section describes the connectors, controls and indicators on the Mosaic DMD Head and relevant signal specifications. (This is common between Mosaic 3 and Mosaic Infinity models). Power Switch External interface Power Ext Trigger...

  • Page 40: External Interface

    Mosaic3 HARDWARE DESCRIPTION 3.1.5 e xternaL nterface The following is a description of the 26-way High Density Female D-type (all other pins are not connected): Figure 12: 26-way High Density Female D-type Signal Ext Trigger DMD Active 17 - 10 Frame Address [7:0] 3, 6, 18, 20, 25, 26 The Frame Address is an 8-bit address input that allows the user to choose which frame is displayed in...

  • Page 41: Section 4: Installation

    Mosaic3 INSTALLATION SECTION 4: INSTALLATION Important: Please move all equipment to the installation area at least 24 hours prior to the arrival of the Installation Technician. This product must be installed by an Installation Technician or a trained Systems distributor authorised by Andor.

  • Page 42: Mosaic Cabling

    Mosaic3 INSTALLATION 4.5 m osaic abLing All device interconnection cables will be installed by the Andor Installation Technician and should not be removed by the user. The External Trigger and DMD Active Input and Output use SMA connectors. If connecting a new cable carefully align the cable’s central pin with the coupler on the Mosaic head then gently insert the cable into the coupler.

  • Page 43: Section 5: Operation

    Mosaic3 OPERATION SECTION 5: OPERATION If the equipment is used in a manner not specified by Andor, the protection provided by the equipment may be impaired. 5.1 e mergency isconnection In case of emergency, the disconnecting point is the mains power cord connected to the external power supply, or the mains socket switch.

  • Page 44: Using The Mosaic

    Mosaic3 OPERATION 5.4 u sing the osaic 5.4.1 c ontroL The device is fully controlled by a software Graphical User Interface (GUI). The features available are unique to the control software used e.g. Andor iQ, MetaMorph or NIS-Elements. Please refer to the applicable User Manual for the software you are using.

  • Page 45: M Ask R Eferencing M Odes

    Mosaic3 OPERATION 5.4.3 m eferencing odes If masks are loaded onto the Mosaic’s on-board memory then there are two ways of referencing which mask to select from memory to expose: Software A software command from the control PC identifies the required mask for Exposure. Hardware The required mask is referenced by an 8 bit binary address input through the D-SUB External Interface Connector.

  • Page 46: I Nvert M Ode

    Mosaic3 OPERATION 5.4.5 i nvert This modifier will invert the binary mask as it uploads it to the Mosaic head. This can be used with both Single Shot and Mask Memory modes. 5.4.6 s equencing Each software GUI will implement sequencing in its own manner, please refer to the applicable User Manual for guidance.

  • Page 47: O Verlap M Ode

    Mosaic3 OPERATION 5.4.7 o verLap This applies to Sequencing operational mode and will remove any gaps between exposures so there will continually be a mask displayed and a minimum possible gap during mask transition. The DMD Active output signal will give a short (~5 µs) dip to allow mask counting or the synchronisation of other hardware.

  • Page 48: Amp Ouse Ilters Not Available On Osaic Nfinity )

    Mosaic3 OPERATION 5.4.12 L ouse iLters not avaiLabLe on osaic nfinity The Lamp House adapters of the Mosaic 3 model have a removable 38 mm filter holder which is usually fitted with a Heat Shield Filter. To remove the lamp house filter, grip the holder and pull upwards as shown in figure 17 below. Figure 14: Heat Shield Filter Warnings: •...

  • Page 49: Conditions Required For Laser Emission

    Mosaic3 OPERATION 5.5 c onditions equired for aser mission If supplied with a laser, laser radiation can only be emitted from the Mosaic when all the conditions below have been satisfied: All cables are correctly connected to the control PC and between the Mosaic and its system components. AC Power is applied to the Mosaic, its system components and the Power Switches (key switch) are ‘ON’.

  • Page 50: C Entration Of The L Aser I Llumination On The Dmd

    Mosaic3 OPERATION While the focus and size are not critical, they deserve some attention. The first image (left) shows a picture of a poorly focused spot and the second (right) shows a sharply focused large spot. Figure 16: (left) Small but poorly focused spot, (right) Larger but sharply focused spot 5.6.2 c entration of the aser...

  • Page 51: C Entration Of The I Llumination To The M Icroscope

    Mosaic3 OPERATION 5.6.3 c entration of the LLumination to the icroscope When a laser illumination source is installed, the microscope will have a Fluorescent Target installed in the nosepiece turret. Rotate the turret to the fluorescent target’s position. In the case of an inverted microscope, remove any slide carrier or shield such that the entire window of the fluorescent target may be viewed.

  • Page 52: Changing The Dichroic Position (Mosaic Infinity)

    Mosaic3 OPERATION 5.7 c hanging the ichroic osition osaic nfinity The Mosaic Infinity Dichroic slider enables the selection between three positions. Move the Slider between the positions as required- . These are defined in the adjacent label (Figure 22) to indicate which dichroic option is available at each position.

  • Page 53: Section 6: Maintenance

    Mosaic3 MAINTENANCE SECTION 6: MAINTENANCE WARNINGS: Important: The system should be powered-down prior to performing the maintenance checks, except for the interlock checks. Contact your Andor Support Representative with any queries or issues. Do not use equipment that is damaged. Leaning and econtamination The most critical aspect of maintenance by the user is to ensure that the system is in a clean environment that is suitable...

  • Page 54: Annual Electrical Safety Checks

    Mosaic3 MAINTENANCE 6.3 a nnuaL LectricaL afety hecks It is advisable to check the integrity of the insulation and protective earth of the product on an annual basis, e.g. U.K. PAT testing. 6.4 c hanging the ichroic osaic nfinity The Mosaic Infinity features a manual dichroic slider (see also section 5.7) enabling the user to switch between the installed dichroic filters.

  • Page 55: Section 7: Troubleshooting

    Mosaic3 TROUBLESHOOTING SECTION 7: TROUBLESHOOTING 7.1 t roubLeshooting xampLes Refer also to the operation and troubleshooting information provided with your other system components. Contact your Andor customer support representative if you require further assistance. Version 3.5 rev 8 May 2019...

  • Page 56: Problem Reporting Form

    Mosaic3 TROUBLESHOOTING 7.2 p robLem eporting Please have the following information when connecting customer support: Owner Information Institution Name Vendor Information Vendor Contact Equipment Information Model Serial No. Check List Cable Connections Mains Power Summary What is the nature of the problem? Any other observations Version 3.5 rev 8 May 2019...

  • Page 57: Section 8: Technical Specifications

    Mosaic3 TECHNICAL SPECIFICATIONS SECTION 8: TECHNICAL SPECIFICATIONS 8.1 ac/dc p oWer uppLy equirements Parameter Specification Mains Input Voltage Range 100 – 240 V a.c. (typical) Mains Input Frequency Range 43 – 67 Hz (typical) Mains Input Steady State Current 0.1 A max. based on typical voltage range above Low Voltage Output 12 V d.c.

  • Page 58: Operating Conditions

    Mosaic3 TECHNICAL SPECIFICATIONS 8.4 o perating onditions The product has been designed for the following operating conditions: Parameter Specification Location Indoor use Operating Temperature +10 to +30 °C Relative Humidity 80 % non-condensing Altitude Up to 2000 m Rated Pollution Pollution Degree 2.

  • Page 59: Appendix A: Mechanical Drawings

    Mosaic3 MECHANICAL DRAWINGS APPENDIX A: MECHANICAL DRAWINGS All Dimensions: mm [inches] Mosaic 3 Head (all models) Mosaic 3 Mosaic 3 Duet Version 3.5 rev 8 May 2019...
  • Page 60 Mosaic3 MECHANICAL DRAWINGS Mosaic Infinity (1.0X) Mosaic Infinity (0.5X) Version 3.5 rev 8 May 2019...

  • Page 61: Appendix B: Glossary

    Mosaic3 GLOSSARY APPENDIX B: GLOSSARY Alternating Current (Mains) AC/DC An electronic device that converts AC electricity (usually mains) into a DC voltage (usually a safe low voltage) Analogue-to-Digital Converter: Converts an analogue voltage to a digital signal. CLSM Confocal Laser Scanning Microscopy Direct Current Digital Micro-mirror Device Electrophysiology...

  • Page 62: Appendix C: Other Information

    Mosaic3 OTHER INFORMATION APPENDIX C: OTHER INFORMATION erms and ondiTions of ale and arranTy nformaTion The terms and conditions of sale, including warranty conditions, will have been made available during the ordering process. The current version may be viewed at: www.andor.com/pdfs/literature/Andor_Standard_Warranty.pdf 2006 (Weee) asTe...

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