GE BioProcess LPLC Operating Instructions Manual page 62

Low pressure liquid chromatography or medium pressure liquid chromatography columns
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5 Operation
5.5 Connecting a packed column
Final considerations
Once the column is connected, the following should be taken into consideration.
62
Make sure to activate the system alarm(s) again after the filling sequence.
When the pump is started, the system must be in Column UpFlow position.
If the column will be run in downwards mode after the connection, the tubing con-
nected to the column top must also be filled with liquid before it is connected to the
column top.
LPLC and MPLC Columns Operating Instructions 28-9638-66 AC

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