Related Manuals for Harvard Apparatus QuikPrep ElectroPrep 74-1196
Summary of Contents for Harvard Apparatus QuikPrep ElectroPrep 74-1196
- Page 1 ElectroPrep System ™ USER’S MANUAL ElectroPrep System 74-1196 Publication 9511-073 REV-1.0...
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Page 2: Table Of Contents
Table of Contents SUBJECT PAGE # Warranty Information Safety Information Product Overview Using the ElectroPrep Unit 7-11 Assembly and Use How to Select Your Chamber and 10-11 Membrane Configuration ElectroPrep Protocols 12-15 Electrodialysis through Simultaneous Exchange of Buffers Selective Electrofiltration/Concentration/Separation Based on Different Charges of Biomolecules Rapid and Selective Electrofiltration or Concentration Electroelution and Electrofractionation of DNA,... -
Page 3: Warranty Information
Harvard Apparatus warranties the QuikPrep ElectroPrep System for a period of one year from the date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials. This warranty does not... - Page 4 Repair service will be billed on the basis of labor and materials. A complete statement of time spent and materials used will be supplied. Shipment to Harvard Apparatus should be prepaid. Your bill will include return shipment freight charges.
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Page 5: Safety Information
Observe all Warning Labels on Product Read all labels on product to ensure proper usage. If there are any questions about the operation of this instrument, call Harvard Apparatus Technical Service at 800-272-2775, or 508-893-8999. ElectroPrep System Publication 9511-073 REV-1.0... - Page 6 Safety Information Caution Notice The QuikPrep ElectroPrep System is intended for laboratory use only and may be used in research and development applications. These systems have been designed to meet the applicable safety requirements for electrical equipment for measurement, control, and laboratory use.
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Page 7: Product Overview
Product Overview The QuikPrep® ElectroPrep™ Electrodialysis System is an extremely versatile patented sample preparation technology that is capable of separating samples by both size and charge. It is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. Membranes of different MWCO (molecular weight cut off), from 100 to 300,000 daltons, can be used for selective buffer exchange, dialysis, filtration, concentration, fractionation and elution. -
Page 8: Using The Electroprep Unit
Using the ElectroPrep Unit Assembly and Use A functional ElectroPrep System consists of the ElectroPrep Tank, power supply, and one or more Dialyzer Units. The ElectroPrep Tank (74-1196) is supplied with a tank, lid and connectors, and a replacement gasket. Power supply and Dialyzer Units must be purchased separately. The ElectroPrep System uses at least one Dialyzer Unit to perform a sample electrodialysis. - Page 9 Using the ElectroPrep Unit Dialyzer Units can be configured in a number of more complex ways to perform different applications using a combination of components: • Dialysis Chamber: the major receptacles for either samples or dialyzed materials. Includes one main chamber with two open ports and two open end caps. The sample chambers are made of PTFE, an inert material especially suited for high sample recovery and are available in a range of 50 µl to 1500 µl volumes.
- Page 10 Using the ElectroPrep Unit • Dialysis Membranes are added at one or both ends or between Dialysis Chamber and/or Link Chambers and Unions. Membranes with molecular weight cut off points (MWCOs), ranging from 500 to 300,000 daltons, may be used in combination with different Dialysis and Link Chambers for selective elution, filtration, dialysis, fractionation and concentration of complex samples.
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Page 11: How To Select Your Chamber And Membrane Configuration
Using the ElectroPrep Unit Open End Caps: Secondary Link Chamber: Primary Link Chamber: Lock membranes Can be connected to a Can be connected to a into position. Primary Link Chamber, and Dialysis Chamber, and joined with an Open End Cap joined with a Secondary Link at the other end. - Page 12 Using the ElectroPrep Unit 3. Choose Dialysis Membranes of suitable size, type and MWCO depending on the application being done and the molecular weight of the biological molecule of interest. a. Membrane Type: Take into account the membrane’s suitability for use in aqueous or organic solvents.
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Page 13: Electroprep Protocols
ElectroPrep Protocols Electrodialysis through Simultaneous Exchange of Buffers (Configuration #1) Applications • Rapidly remove 100% of primer after PCR reaction (5 to 10 minutes). • De-salting of neutral materials that do not move in an electric field (such as sugars) or charged molecules at their isoelectric point. -
Page 14: Selective Electrofiltration/Concentration/Separation Based On Different Charges Of Biomolecules
ElectroPrep Protocols Selective Electrofiltration/Concentration/Separation Based on Different Charges of Biomolecules (Variation of Configuration #2) Applications • Separation and purification of biomolecules with unknown isoelectric potential In Configuration #2 the sample is placed in a union between two membranes (b), both of which should have a MWCO larger than the desired biomolecules. -
Page 15: Rapid And Selective Electrofiltration Or Concentration
ElectroPrep Protocols Rapid and Selective Electrofiltration or Concentration (Variation of Configuration #2) Applications • Concentration of small samples and/or selective filtration In this Configuration #2 variant, the sample is placed in the sample compartment comprised of a Dialysis Chamber (1) and the Union connected to a receiving Dialysis Chamber (2) of same or smaller volume. The MWCO of membrane (b) should be larger than the molecular weight of the biomolecules and the MWCO of membranes (a) and (c) should be smaller. -
Page 16: Electroelution And Electrofractionation Of Dna
ElectroPrep Protocols Electroelution and Electrofractionation of DNA, Proteins or Other Biomolecules from Gel Pieces (Variation of Configuration #2) Applications • Elution of DNA, proteins, or other biomolecules from one or more gel pieces. Optionally, electrofraction of eluted biomolecules by size may be done at the same time. Using the ElectroPrep system in this Configuration #2 variant, elution of DNA, proteins, or any other biomolecules from a gel slice/plug can be achieved quickly and easily with excellent recovery. -
Page 17: Specifications
Specifications TECHNICAL SPECIFICATIONS Maximum Power 50 W Maximum Voltage 1000 V Maximum Current 500 mA Maximum Liquid Temperature 45°C Unit Dimensions (L x H x W) 9.8 x 5.2 x 5.5 in (25 x 13.2 x 13.14 cm) Weight 1.9 lb (0.86 kg) Maximum Buffer Volume 1000 ml Power Output Connectors... -
Page 18: General Care & Maintenance
DISTILLED WATER ONLY to the maximum fill line. Any leakage will be seen on the tissue paper. If any leakage is seen DO NOT ATTEMPT TO REPAIR OR USE THE APPARATUS, but notify Harvard Apparatus immediately. • The replacement platinum electrodes are partially shrouded for protection. However, when cleaning the main tank DO NOT use cleaning brushes in the electrode area. -
Page 19: Ordering Information
Ordering Information Description Item # ElectroPrep Hardware ElectroPrep Tank, with lid, gasket, 4 mm red and black 74-1196 connector cables ElectroPrep Replacement Connector Cables, 4 mm, red and 74-1197 black (1 each) Power Supply for Electroprep, 300 V, 500 mAmp, 90W 74-1103 Replacement Gasket, Qty. - Page 20 Ordering Information Description Item # 25 kDa MWCO 7410-RC25K 50 kDa MWCO 7410-RC50K Cellulose Acetate 500 Da MWCO 7410-CA500 1 kDa MWCO 7410-CA1K 2 kDa MWCO 7410-CA2K 5 kDa MWCO 7410-CA5K 10 kDa MWCO 7410-CA10K 25 kDa MWCO 7410-CA25K 50 kDa MWCO 7410-CA50K 100 kDa MWCO 7410-CA100K...
- Page 21 Ordering Information Description Item # 10 kDa MWCO 7416-CA10K 25 kDa MWCO 7416-CA25K 50 kDa MWCO 7416-CA50K 100 kDa MWCO 7416-CA100K 300 kDa MWCO 7416-CA300K Polycarbonate 0.01 µm Pore Size 7416-PC01 0.05 µm Pore Size 7416-PC05 0.10 µm Pore Size 7416-PC10 0.60 µm Pore Size 7416-PC60...
- Page 22 Ordering Information Description Item # Polycarbonate 0.01 µm Pore Size 7425-PC01 0.05 µm Pore Size 7425-PC05 0.10 µm Pore Size 7425-PC10 0.60 µm Pore Size 7425-PC60 ElectroPrep Membranes for Secondary Link Chambers (50 to 100 µl, 7/16” Diameter) Type and MWCO Regenerated Cellulose 1 kDa MWCO 7424-RC1K...
- Page 23 Notes ElectroPrep System Publication 9511-073 REV-1.0...
- Page 24 Notes ElectroPrep System Publication 9511-073 REV-1.0...
- Page 25 E-mail support@hbiosci.com www.harvardapparatus.com Spain Panlab S.L.U. Canada C/ Energia, 112 08940 Cornellà (Barcelona), Spain Harvard Apparatus, Canada Phone +46 8 470 10 00 6010 Vanden Abeele Street Saint Laurent, Quebec, H4S 1R9 +46 8 470 10 50 Phone (514) 335-0792 E-mail info@panlab.com...