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ElectroPrep Protocols
Electroelution and Electrofractionation of DNA, Proteins or Other
Biomolecules from Gel Pieces (Variation of Configuration #2)
Applications
• Elution of DNA, proteins, or other biomolecules from one or more gel pieces. Optionally, electrofraction
of eluted biomolecules by size may be done at the same time.
Using the ElectroPrep system in this Configuration #2 variant, elution of DNA, proteins, or any other biomolecules
from a gel slice/plug can be achieved quickly and easily with excellent recovery. Using a Union, Chambers can be
joined in any combination necessary to accommodate the required gel volume. Samples can be concentrated if
desired, by choosing a receiving Chamber of suitable smaller volume. The MWCO of the membranes (a and b) can
also be chosen to achieve very selective filtration or size fractionation during the electroelution process.
Steps
1. Choose desired elution and sample Dialyzer Chambers, Union and Membranes.
2. Assemble Dialyzer Unit (beginning with Dialyzer Chamber that will be the elution sample collection
chamber). Place Membrane (a), lower MWCO than your biomolecule, on the membrane platform of the
Dialyzer chamber (2) and add an Open End Cap (hand tighten).
3. Add buffer to the elution sample collection Dialyzer Chamber.
4. Place membrane (b), MWCO larger than your biomolecule, into the elution sample collection Dialyzer
Chamber. Add Union onto other end.
5. Assemble second Dialyzer Chamber (1) to the Union to create a combined large compartment, and add gel
slice(s) and buffer to the combined large compartment.
6. Place your next Membrane (c), lower MWCO than your biomolecule on the other side of the combined
large compartment and assemble End Cap.
7. Gently push the assemblage of chambers through the ElectroPrep tank gasket to secure the dialysis unit in place.
8. Fill the Electroprep tank with electroelution buffer and ensure the Dialysis Chambers are covered, but the
fluid does not flow over the partition. Chambers and caps must be completely immersed in buffer so that
no air bubbles are present. Keeping all parts submersed in buffer prevents the introduction of air bubbles
into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Set current and voltage settings as
required for the sample, gel, and buffer (15 mA suggested as a starting point). Elution time can be
calculated by measuring the time required for the biomolecule to migrate 1 cm during gel electrophoresis.
Electro-Elution of DNA,
Proteins or Other Biomolecules
from Gel Pieces
ElectroPrep System
Publication 9511-073 REV-1.0
Key
ElectroPrep Dialyzer
Open End Cap
Membrane
Union
Gel
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