Product Overview - Harvard Apparatus QuikPrep ElectroPrep 74-1196 User Manual

Table of Contents

Advertisement

Product Overview

The QuikPrep® ElectroPrep™ Electrodialysis System is an extremely versatile patented
sample preparation technology that is capable of separating samples by both size and
charge. It is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and
other biomolecules. Membranes of different MWCO (molecular weight cut off), from
100 to 300,000 daltons, can be used for selective buffer exchange, dialysis, filtration,
concentration, fractionation and elution.
The ElectroPrep System provides faster dialysis times due to movement of charged
molecules in an electric field during dialysis, thus combining electrophoresis with dialysis.
With a run time of 5 to 10 minutes, ElectroPrep provides speed and convenience, even at
the very low currents (5 to 10 mA) used with this system.
Applications
• Electroelution from gels and solutions, e.g. gel extraction of vector and
insert during cloning
• Electrodialysis (with an average buffer exchange time of 5 to 10 minutes)
• Electroconcentration
• Selective electrofiltration
• Size fractionation of DNA and proteins from complex lysates
• Primer removal following PCR amplification
• Salt removal from DNA mini-preps
• Detergent removal
• Dye-terminator removal
ElectroPrep System
6
Publication 9511-073 REV-1.0

Advertisement

Table of Contents
loading

Table of Contents