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ElectroPrep Protocols
Electrodialysis through Simultaneous Exchange of Buffers
(Configuration #1)
Applications
• Rapidly remove 100% of primer after PCR reaction (5 to 10 minutes).
• De-salting of neutral materials that do not move in an electric field (such as sugars)
or charged molecules at their isoelectric point.
In this configuration, a sample is placed in the Dialysis Chamber between Membranes (a) and (b),
both of which have MWCOs lower than the molecular weight of the desired biomolecules. The
sample is dialyzed through the simultaneous exchange of buffers in the electric field. This method is
very fast and very effective.
Steps
1. Select the proper size Dialyzer Chamber to use as much of the available chamber volume as
possible (minimize air gaps).
2. Chose membranes, (a) and (b), that have MWCOs lower than the molecular weight of the
biomolecule(s) of interest.
3. Assemble Dialyzer Unit by placing one membrane on the platform of the Dialyzer Chamber and
hand-tightening one End Cap.
4. Place the sample in the Dialysis Chamber between the two membranes.
5. Place the second membrane and assemble the second End Cap to create the completed Dialysis
Chamber unit.
6. Install the Dialysis Chamber unit into the tank by gently pushing the unit through the ElectroPrep
tank gasket to secure in place.
7. Fill the Electroprep tank with buffer and ensure the Dialysis Chamber is covered, but the buffer does
not flow over the partition. Dialysis Chamber and caps must be completely immersed in buffer so
that no air bubbles are present. Keeping all parts submersed in buffer prevents the introduction of air
bubbles into the Dialyzer Chambers.
8. Assemble the lid with cables and connect to your power supply. Use current and voltage settings as
required for the sample and buffer (5 to 10 mA suggested as a starting point).
12
Electrodialysis
through Simultaneous
Exchange of Buffers
ElectroPrep System
Publication 9511-073 REV-1.0
Key
ElectroPrep Dialyzer
Open End Cap
Membrane
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