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ElectroPrep Protocols
Rapid and Selective Electrofiltration or Concentration
(Variation of Configuration #2)
Applications
• Concentration of small samples and/or selective filtration
In this Configuration #2 variant, the sample is placed in the sample compartment comprised of a Dialysis
Chamber (1) and the Union connected to a receiving Dialysis Chamber (2) of same or smaller volume. The
MWCO of membrane (b) should be larger than the molecular weight of the biomolecules and the MWCO
of membranes (a) and (c) should be smaller. Upon the passage of electric current, the biomolecules will
pass through membrane (b) and collect in Dialysis Chamber (2) while smaller molecules will continue to
pass through membrane (b) and (a). This is a fast and effective method for selective filtration, and for
concentrating small samples.
Steps
1. Select the proper size Dialyzer Chamber to use as much of the available chamber volume as possible
(minimize air gaps).
2. Assemble Dialyzer Unit by placing one membrane (a) on the platform of the Dialyzer Chamber (2)
and hand-tightening an End Cap.
3. Add buffer to the Dialyzer Chamber.
4. Place your next membrane (b) on the other side of the Dialyzer Chamber and assemble the Union to
create a combined large compartment.
5. Assemble second Dialyzer Chamber (1) to the Union and add your sample to the combined
large compartment.
6. Place your next membrane (c) on the other side of the combined chamber and assemble End Cap.
7. Gently push the assemblage of chambers through the ElectroPrep tank gasket to secure the dialysis
unit in place.
8. Fill the Electroprep tank with electroelution buffer and ensure the Dialysis Chambers are covered,
but the fluid does not flow over the partition. Chambers and caps must be completely immersed
in buffer so that no air bubbles are present. Keeping all parts submersed in buffer prevents the
introduction of air bubbles into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Use current and voltage settings as
required for the sample and buffer (5 to 10 mA suggested as a starting point). Elution time can be
calculated by measuring the time required for the biomolecule to migrate 1 cm during gel electrophoresis.
14
Rapid and Selective
Electro-Filtration or
Concentration
ElectroPrep System
Publication 9511-073 REV-1.0
Key
ElectroPrep Dialyzer
Open End Cap
Membrane
Union
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