Appendix C Evos Light Cubes; Led Illumination; Evos ™ Light Cubes - Thermo Scientific Invitrogen Countess 3 FL User Manual

C

LED illumination

™
The Countess
by the proprietary, user-interchangeable LED light cube (see "EVOS
Because the LED light source is as close as possible to the objective, the number of optical elements
in the channel is minimized. High-intensity illumination over a short channel increases the efficiency of
fluorophore excitation, providing better detection of weak fluorescent signals.
™
EVOS light cubes
Each user-interchangable, auto-configured EVOS
filters. In addition to the brightfield channel dedicated to cell count and cell viability assays using trypan
blue, the Countess
multiple-fluorescence research applications
The following table lists some of the common fluorescent and specialty EVOS
from Thermo Fisher Scientific. For a complete list, go to
contact Technical Support. For instructions on changing the LED light cubes, see Chapter 10, "Change
EVOS ™ light cubes".
Light Cube
DAPI
TagBFP
CFP
GFP
YFP
Countess ™ 3 FL Automated Cell Counter User Guide
3 FL Automated Cell Counter utilizes an adjustable intensity LED light source provided
™
3 FL Automated Cell Counter can accommodate two fluorescent light cubes for
DAPI, Hoechst
TagBFP
ECFP, Lucifer Yellow, Evans Blue
GFP, Alexa Fluor
EYFP, acridine orange + DNA
EVOS light cubes
™
light cubes"
™
light cube contains an LED, collimating optics, and
www.thermofisher.com/evoslightcubes
Dyes
™
, BFP
™
488, SYBR GREEN
on page 61).
™
light cubes available
™
, FITC
or
61