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Summary of Contents for Molecular Devices FilterMax F3
- Page 1 FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Readers User Guide 5016208 D October 2018...
- Page 2 For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
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Page 3: Table Of Contents
Contents Safety Information Warnings, Cautions, Notes, and Tips Chapter 1: Introduction Computer Integration Temperature Control Chapter 2: Setting Up the Instrument Package Contents Unpacking the Instrument Removing Transport Locks Connecting Instrument Cables Assembling and Maintaining Filters Chapter 3: Getting Started Front Panel Controls and Indicators Loading and Unloading Filter Slides Loading and Unloading Plates... -
Page 4: Safety Information
Follow the related safety information. The following user attention statements might be displayed in the text of Molecular Devices user documentation. Each statement implies the amount of observation or recommended procedure. - Page 5 For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device. 5016208 D...
- Page 6 Electrical Safety To prevent electrical injuries and property damage, inspect all electrical equipment before use and report all electrical deficiencies. Contact Molecular Devices technical support for equipment service that requires the removal of covers or panels. WARNING! HIGH VOLTAGE.
- Page 7 Safety Information WARNING! Never use the instrument in an environment where potentially damaging liquids or gases are present. Moving Parts Safety The instrument contains moving parts that can cause injury. Under normal conditions, the instrument is designed to protect you from these moving parts. WARNING! If the instrument is used in a manner not specified by Molecular Devices, the protection provided by the equipment might be impaired.
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Page 8: Chapter 1: Introduction
Chapter 1: Introduction The FilterMax™ F3 Multi-Mode Microplate Reader and the FilterMax™ F5 Multi-Mode Microplate Reader are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. The unique patented design ensures precise performance and sensitivity across all detection modes. The instrument has two slots where you insert filter slides. -
Page 9: Computer Integration
Protect skin from exposure to the light emitted by the lamp. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. -
Page 10: Temperature Control
Chapter 1: Introduction Note: For information about the computer specifications that are required to run the software, the software installation and licensing instructions, and the directions to create the software connection between the computer and the instrument, see the SoftMax Pro Data Acquisition and Analysis Software Installation Guide. To prevent data loss, turn off all sleep and hibernation settings for the hard disk, the CPU, and the USB ports. -
Page 11: Chapter 2: Setting Up The Instrument
Chapter 2: Setting Up the Instrument Before you unpack and set up the instrument, prepare a dry, flat work area that has sufficient space for the instrument, host computer, and required cables. See Instrument Specifications on page Package Contents Illustration Part Number Description SoftMax®... -
Page 12: Unpacking The Instrument
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Unpacking the Instrument The packaging is designed to protect the instrument during transportation. There is a transport lock on the plate drawer to protect the instrument from damage during shipment. You must remove the transport lock before you power on the instrument. - Page 13 Chapter 2: Setting Up the Instrument Item Description 1 Screw #1 fastens the lock to the internal frame of the instrument 2 Screw #2: Fastens the lock to the plate drawer 3 Screw #3: Fastens the lock to the plate drawer 4 ...
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Page 14: Connecting Instrument Cables
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. Required Accessories Illustration Part Number Description GU V3 244 RS-232 9-pin serial connection cable... -
Page 15: Assembling And Maintaining Filters
Chapter 2: Setting Up the Instrument To connect the instrument cables: 1. Make sure the power switch on the rear of the instrument is in the Off position. See Getting Started on page 2. Connect one end of the supplied USB cable to the USB port on the rear of the instrument and the other end to a USB port on the computer. - Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. Use the 1.5 mm hex key to loosen, but not remove, the hex screw that secures the filter to the filter slide.
- Page 17 Chapter 2: Setting Up the Instrument 4. Hold the new filter by its edges and gently place it in the slot. Excitation and emission filters are different sizes to prevent installation on the wrong type of filter slide. Note: Some excitation filters have a heat reflecting coating on one side that must face the light source.
- Page 18 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide CAUTION! Handle filter slides by the tab only. Do not touch filter surfaces with fingers. Fingerprints left on filters can cause inaccurate measurement results. To remove or install a polarization filter: 1.
- Page 19 Chapter 2: Setting Up the Instrument Cleaning Filters You can clean excitation and emission filters. To minimize the risk of surface damage, clean the filters only when the surface is noticeably dirty. CAUTION! Filter surfaces are extremely fragile and should be cleaned only when noticeable contamination, such as dust and fingerprints, are visible on the surface.
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Page 20: Chapter 3: Getting Started
Chapter 3: Getting Started Now that you installed the SoftMax Pro Software on the computer, unpacked the instrument, removed the transport lock, and connected the cables, it is time to get started. The power switch and power connection are on the rear of the instrument. Item Description 1 ... -
Page 21: Front Panel Controls And Indicators
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Front Panel Controls and Indicators The instrument front panel has three buttons and LED status indicators that provide information about instrument status. The buttons enable you to open and close the plate drawer and eject the filter slides. - Page 22 Chapter 3: Getting Started Front Panel Indicators and Controls Item Description 1 Green LED 2 Amber LED 3 - Open and close plate drawer 4 - Eject excitation filter slide EX FILTER 5 - Eject emission filter slide EM FILTER To stop the instrument in case of emergency, simultaneously press the EX FILTER buttons.
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Page 23: Loading And Unloading Filter Slides
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Loading and Unloading Filter Slides The buttons on the instrument front pane enable you to exchange filter slides at any time between reads. Each filter slide has an identification code built into the slide that enables the SoftMax Pro Software to recognize the filter configuration. -
Page 24: Loading And Unloading Plates
Chapter 3: Getting Started To load filter slides: 1. Power on the instrument and wait until the initialization process completes. 2. Open the filter compartment door. 3. Grasp the filter slide by the tab. Excitation filter slides have EX or EXP printed on the slide tab. Emission filter slides have EM or EMP printed on the slide tab. - Page 25 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Plate Drawer With Plate Loaded Item Description 1 open and close the plate drawer 2 Well A1 on the plate for landscape orientation 3 Plate drawer 3. Press to close the plate drawer.
- Page 26 Chapter 3: Getting Started Plate Recommendations The instrument can read SBS standard plates. The FilterMax F3 can read 96-well and 384-well plates. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well plates. Note: When an application specifies a surface treatment, use only plates with the correct treatment.
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Page 27: Chapter 4: Maintenance And Troubleshooting
Chapter 4: Maintenance and Troubleshooting Perform only the maintenance tasks described in this guide. Contact a Molecular Devices service engineer to inspect and perform a preventive maintenance service on the instrument each year. See Obtaining Support on page Before operating the instrument or performing maintenance operations, make sure you are familiar with the safety information in this guide. -
Page 28: Replacing Fuses
If these checks fail to remedy the loss of power, replace the fuses. You can obtain replacement fuses from Molecular Devices. For fuse specifications and part numbers, see Physical Specifications on page CAUTION! Do not touch or loosen screws or parts other than those specifically designated in the instructions. - Page 29 7. Plug the power cord into the power port. 8. Power on the instrument. Note: If the instrument still does not power on after you change the fuses, contact Molecular Devices technical support. See Obtaining Support on page 5016208 D...
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Page 30: Replacing The Deuterium Lamp In The Filtermax F5
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Replacing the Deuterium Lamp in the FilterMax F5 The FilterMax F5 has a deuterium lamp for use in measurements made at wavelengths between 230 nm and 359 nm. You need to replace the lamp when the SoftMax Pro Software warns that lamp energy is too low to perform measurements. - Page 31 Chapter 4: Maintenance and Troubleshooting 4. On the top of the instrument case, use the 2 mm hex key to remove the two hex screws that secure the lamp housing cover. Item Description 1 Lamp housing cover 2 Hex screws 3 Lamp power cable and connector 4 ...
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Page 32: Packing The Instrument
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide 8. After the deuterium lamp cools, remove the lamp and discard it. CAUTION! Do not touch the glass of the new lamp. Fingerprints on the glass can shorten the life of the lamp. - Page 33 Chapter 4: Maintenance and Troubleshooting To pack the instrument: 1. Follow applicable decontamination procedures as instructed by your laboratory safety officer. 2. On the front panel of the instrument, press to eject the excitation filter slide. EX FILTER Item Description 1 ...
- Page 34 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide 7. On the front panel of the instrument, press to move the plate drawer outside of the instrument. 8. Remove the plate from the drawer and leave the plate drawer open.
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Page 35: Troubleshooting
21. Close the box and seal it with packing tape. Troubleshooting CAUTION! Maintenance procedures other than those specified in this guide must be performed by Molecular Devices. When service is required, contact Molecular Devices technical support. Then The green LED is not Inspect the fuses. -
Page 36: Obtaining Support
WARNING! BIOHAZARD. It is your responsibility to decontaminate components of the instrument before you return parts to Molecular Devices for repair. Molecular Devices does not accept items that have not been decontaminated where it is applicable to do so. If parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated. -
Page 37: Appendix A: Instrument Specifications
Appendix A: Instrument Specifications This appendix provides specifications for the FilterMax F3 and FilterMax F5. Measurement Specifications Table A-1: Absorbance Measurement Specifications Item Description Wavelength range 340 nm to 650 nm (FilterMax F3) Wavelength range 230 nm to 650 nm (FilterMax F5) Accuracy <±2.0 nm and ±10 mOD at 2.0 OD (at 405 nm) Reproducibility <±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm) <±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm) - Page 38 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Table A-2: Fluorescence Intensity Top Measurement Specifications Item Description Wavelength range Excitation: 340 nm to 630 nm (FilterMax F3) Emission: 390 nm to 650 nm Wavelength range Excitation: 230 nm to 650 nm (FilterMax F5) Emission: 390 nm to 700 nm Detection limit, guaranteed 5 fmol/200 μL fluorescein (25 pM), black 96-well plate;...
- Page 39 Appendix A: Instrument Specifications Table A-3: Fluorescence Intensity Bottom Measurement Specifications (FilterMax F5) Item Description Wavelength range Excitation: 300 nm to 630 nm Emission: 390 nm to 650 nm Detection limit, guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL fluorescein (200.3 pM), black 384-well plate; 400 ms integration time Detection limit, optimized 8 fmol/200 μL fluorescein (40 pM), black 96-well plate;...
- Page 40 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Table A-5: Fluorescence Polarization Specifications (FilterMax F5) Item Description Wavelength range Excitation: 300 nm to 650 nm Emission: 390 nm to 700 nm Uniformity, guaranteed 3 mP at 10 nM fluorescein, black 96-well plate; 1 second integration time 4 mP at 10 nM fluorescein, black 384-well plate;...
- Page 41 Filter slide positions Supplied wavelengths Excitation: 370 nm Emission: 625 nm Note: TRF measurements can be done only with the 370 nm broadband filter supplied by Molecular Devices. Use of this filter is required to achieve the specified performance. Light source High power LEDs Detector...
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Page 42: Physical Specifications
FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Physical Specifications Physical Specifications For The FilterMax F3 and FilterMax F5 Item Description Environment Indoor use only Power requirements 100 VAC to 240 VAC ±10%, 3 amps, frequency range: 50/60 Hz Dimensions 39 cm W x 24 cm H x 58 cm D (15.4 in. -
Page 43: Appendix B: System Diagram And Dimensions
Appendix B: System Diagram and Dimensions Dimensions of the FilterMax F3 and FilterMax F5 Item Description 1 Width: 39 cm (15.4 in.) 2 Height: 24 cm (9.5 in.) 3 Depth: 58 cm ( 22.9 in.) 5016208 D... -
Page 44: Appendix C: Available Standard Filter Slides
The following is a partial list of the standard filter slides that are available from Molecular Devices for the FilterMax F3 and FilterMax F5. Note: You should not reconfigure standard filter slides. FilterMax F3 Standard Filter Slides EX3 Excitation Filter Slide Position Wavelength Read Mode... - Page 45 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide EXP 1 Excitation Filter Slide (Polarization Capable) Position Wavelength Read Mode Status 535 nm Enabled 485 nm Enabled 485 nm Enabled 0 nm Empty 370 nm Enabled 0 nm Empty EMP 1 Emission Filter Slide (Polarization Capable)
- Page 46 Appendix C: Available Standard Filter Slides EX 6 Genomic Filter Slide With PathCheck (Part Number 5011297) Position Wavelength Read Mode Status 260 nm Enabled 280 nm Enabled 320 nm Enabled 0 nm Empty 900 nm PathCheck Enabled 998 nm PathCheck Enabled EX 7 PathCheck filter Slide I (Part Number 5011295) Position Wavelength Read Mode...
- Page 47 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide Available Validation Packages Validation Packages Part Numbers Part Item Name Compatible Instruments Number 0200- SpectraTest ABS1 SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 6117 Absorbance Validation 384, 340PC 384, 190, ABS, ABS Plus, VersaMax, FlexStation 3...
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Page 48: Appendix D: Electromagnetic Compatibility
Appendix D: Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ISM Equipment Classification (Group 1, Class A) This equipment is designated as scientific equipment for laboratory use that intentionally generate and/or use conductively coupled radio-frequency energy for internal functioning, and are suitable for use in all establishments, other than domestic and those directly connected to a low voltage power supply network which supply buildings used for domestic... - Page 49 The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. ©2018 Molecular Devices, LLC. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents All rights reserved. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES...
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