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CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experi- ence, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for com- petitive purposes, nor made available to third parties.
Scope of delivery .......................... 22 Technical data ........................... 22 3.5.1 FatExtractor E-500......................... 22 3.5.2 Ambient conditions......................... 23 3.5.3 Materials .......................... 23 Transport and storage ...................... 24 Transport ............................ 24 Storage .............................. 24 Lifting the instrument ......................... 24 Operation Manual FatExtractor E-500...
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Operating the interface pro .................... 48 Layout of the interface ........................ 48 Function bar............................ 49 Menu bar............................ 49 7.3.1 Home menu ........................... 50 7.3.2 Method menu ......................... 51 7.3.3 Configuration menu........................ 51 7.3.4 Solvent menu ......................... 53 Status bar ............................ 53 Operation Manual FatExtractor E-500...
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Requirements for local network settings.................... 70 11.2 Preparing the instrument for app using.................... 70 11.3 Connecting the instrument and app using a QR code ............... 70 11.4 Connecting the instrument and app using a password.............. 71 Operation Manual FatExtractor E-500...
The following symbols are displayed in this operation manual or on the device: 1.2.1 Warning symbols Symbol Meaning General warning Hot surface Breakable items Hand injuries 1.2.2 Mark-ups and symbols NOTE This symbol draws attention to useful and important information. Operation Manual FatExtractor E-500 7/84...
Signals are marked-up like this. Trademarks Product names and registered or unregistered trademarks that are used in this docu- ment are used only for identification and remain the property of the owner in each case. 8/84 Operation Manual FatExtractor E-500...
Safety | 2 Safety Proper use The FatExtractor E-500 is designed for the extraction (solid-liquid extraction) of sam- ples with known solvents and the reduction or drying of the extract. The FatExtractor E-500 can be used in laboratories for the following tasks: ...
Safety-related incidents that occur while using the device should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.
If faults occur: Switch off the device immediately. Unplug the power cord. Inform the operator. Cool the device before taking any further action. Do not continue to use devices that are damaged. Operation Manual FatExtractor E-500 11/84...
Technical modifications to the device or accessories should only be carried out with the prior written approval of BÜCHI Labortechnik AG and only by authorized BUCHI technicians. BUCHI accepts no liability whatsoever for damage arising as a result of unauthorized modifications. 12/84...
BÜCHI Labortechnik AG Product description | 3 Product description Description of function The FatExtractor E-500 is designed to carry out the following solid-liquid extraction methods: Soxhlet Extraction (see Chapter 3.1.1 "Soxhlet Extraction", page 13) Hot Extraction (see Chapter 3.1.2 "Hot Extraction", page 14) ...
The Analyte Protection protects the analyte from overheating. NOTE The presence of beaker and solvent is always detected wether the Analyte Protec- tion is activated or deactivated. The Analyte Protection triggers under the following situations: 14/84 Operation Manual FatExtractor E-500...
3.2.3 Upper connections on the rear side Fig. 4: Connections on the top Standard BUCHI communication port Standard BUCHI communication port (COM) (COM) LAN port Port for the cooling water valve Operation Manual FatExtractor E-500 17/84...
Coolant water solvent tank in Solvent in Fuse Power supply connection Fuse Inert gas connection (option) Coolant water out Exhaust Coolant water in 10 Type plate 11 Coolant water solvent tank out 12 Solvent out 18/84 Operation Manual FatExtractor E-500...
Product description | 3 3.2.7 Pro Interface Fig. 8: Interface Pro Stop button Touch-screen display Navigation control Type plate The type plate identifies the instrument. The type plate is located at the rear of the in- strument. Operation Manual FatExtractor E-500 21/84...
Overvoltage category IP Code IP20 Pollution degree Protection Class Dimensions (W x D x H) 638 x 595 x 613 mm Weight 42 kg Total heating power 600 W (rated) Total heating power 1200 W (maximum) 22/84 Operation Manual FatExtractor E-500...
The instrument should be transported by two persons at the same time. Lift the instrument at the points indicated. NOTICE Dragging the instrument can damage the feet of the instrument. Lift the instrument when positioning or re-locating. 24/84 Operation Manual FatExtractor E-500...
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BÜCHI Labortechnik AG Transport and storage | 4 Fig. 10: Lifting the instrument Lift the instrument – this requires two persons each lifting at the points indicated on the left and right side of the instrument. Operation Manual FatExtractor E-500 25/84...
1. Provide the mains voltage and frequency specified on the type plate of the instru- ment. 2. Be designed for the load imposed by the instruments connected. 3. Be equipped with suitable fuses and electrical safety features. 4. Be equipped with a proper grounding. 26/84 Operation Manual FatExtractor E-500...
Risk of property damage and diminished performance due to use of un- suitable power cables. The power supply cables supplied with the product by BUCHI precisely match the re- quirements of the device. If other power cables that do not meet those requirements are used, the device may be damaged and/or its performance diminished.
Attach the solvent connection nuts onto the connection marked OUT and IN (SOLVENT) . Attach the inlet tube onto the connection marked OUT . Attach the drain tube onto the connection marked IN (SOLVENT) . 28/84 Operation Manual FatExtractor E-500...
Risk of broken parts by using tools Using tools for preparing the instrument can break parts. Tighten all parts with your hands. Switch the On/Off master switch to Off. Put the bottle cap on the bottle. Operation Manual FatExtractor E-500 29/84...
Condenser rack, chamber rack and beaker rack are already installed according to the ordered method. For Soxhlet Extraction use the following positions: Device Position on the installation board Beaker rack lower Chamber rack Nr. 4 Condenser rack Nr. 2 30/84 Operation Manual FatExtractor E-500...
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See Chapter 5.9.3 "Preparing the chamber for a Soxhlet Extraction", page 32 Install the protection shields. See Chap- ter 5.12.1 "Installing two protection shields (Soxhlet only)", page 37 Select the Soxhlet method on the interface. See Chapter 5.14 "Selecting a configuration", page 41 Operation Manual FatExtractor E-500 31/84...
Attach the sealing to the extraction glass chamber. The extraction glass chamber is prepared. 5.9.3 Preparing the chamber for a Soxhlet Extraction Prepare the extraction glass chamber Soxhlet. See Chapter 5.9.2 "Preparing the extraction glass chamber Soxhlet", page 32 32/84 Operation Manual FatExtractor E-500...
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Put the extraction glass chamber in the cham- ber rack. Turn the extraction glass chamber 90° coun- terclockwise. Make sure, that the extraction glass chamber is installed correctly. Connect the valve with the connection on the installation board. Operation Manual FatExtractor E-500 33/84...
Preparing the instrument Switch the On/Off master switch to Off. Attach the condenser rack to the instrument with screws. Prepare the condenser. See Chapter 5.13 "Preparing the condenser", page 39 Attach the beaker rack to the instrument with screws. 34/84 Operation Manual FatExtractor E-500...
Preparing the instrument Switch the On/Off master switch to Off. Attach the condenser rack to the instrument with screws. Prepare the condenser. See Chapter 5.13 "Preparing the condenser", page 39 Attach the chamber rack to the instrument with screws. Operation Manual FatExtractor E-500 35/84...
Attach the sealing to the extraction glass chamber. 5.11.3 Preparing the Economic Continuous Extraction chamber Prepare the extraction glass chamber. See Chapter 5.11.2 "Preparing the extraction glass chamber for an Economic Continuous Extrac- tion", page 36 Pull the chamber rack. 36/84 Operation Manual FatExtractor E-500...
Push the chamber rack in the instrument. 5.12 Installing the protection shields 5.12.1 Installing two protection shields (Soxhlet only) Make sure, that both protections shields fit to- gether. Hold both protection shields on both sides at the point indicated. Operation Manual FatExtractor E-500 37/84...
Put the front protection shield on both sides into the front slot at the same time. 5.12.2 Installing one protection shield (Hot and Economic Continuous Extraction only) Hold the protection shield on both sides at the point indicated. 38/84 Operation Manual FatExtractor E-500...
Risk of broken parts by using tools Using tools for preparing the instrument can break parts. Tighten all parts with your hands. Attach the solvent connection nut to the condenser. Connect the solvent collector tube. Operation Manual FatExtractor E-500 39/84...
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5 | Installation BÜCHI Labortechnik AG Put the condenser in the condenser rack. Secure the condenser with the flange lock. Turn the condenser. Connect the solvent collector tube to the installation board 40/84 Operation Manual FatExtractor E-500...
ð The display shows a dialog with the selectable configurations. Tap the configuration according to the installed glass setup. ð The configuration is selected. ð The dialog box closes. 5.15 Disassembling the condenser circuit Disconnect the indicated condenser connec- tion. Operation Manual FatExtractor E-500 41/84...
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Make sure, that there is no coolant in the con- denser circuit. Disconnect the other condenser connections. Remove the flange lock. Pull the condenser out of the condenser rack. Disconnect the solvent collector hose. Disconnect the solvent collector tube from the installation board. 42/84 Operation Manual FatExtractor E-500...
Pressing the control performs the assigned function on the function bar. Function bar The function bar shows available functions according to the current operation. The functions on the function bar are executed by tapping the relevant function but- tons. Operation Manual FatExtractor E-500 43/84...
Meaning Submenu/Actions Process control param- [ Home ] menu eters Editing and saving ex- [ Method ] menu traction methods Change settings [ Configuration ] menu Service menu System information 44/84 Operation Manual FatExtractor E-500...
The following configurations are method available: SOX (Soxhlet Extraction) / HE (Hot Extraction) / ECE (Economic Continuous Extraction) [ Max. Cycle time ] Enter value Maximum cycle time in minutes: 0 - (Soxhlet Extraction only) Operation Manual FatExtractor E-500 45/84...
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Explanation [ Sensors ] View All sensor signals can be observed: Flow senors Level sensors Analyte protection (option) Protection shield Valves Lift current Line voltage Line frequency 46/84 Operation Manual FatExtractor E-500...
Solvent menu The solvent menu contains the available solvents for the extraction methods. The solvent menu allows editing individual solvents. See Chapter 10 "Editing a sol- vent", page 69 Status bar Shows the actually used solvent and configuration. Operation Manual FatExtractor E-500 47/84...
The valves close. The heaters switch off. The coolant keep running 5 min. Navigation control Used for navigating the user interface. Pressing the control performs the assigned function on the function bar. 48/84 Operation Manual FatExtractor E-500...
Meaning Submenu/Actions Process control param- [ Home ] menu eters Editing and saving ex- [ Method ] menu traction methods Change settings [ Configuration ] menu Service menu System information Operation Manual FatExtractor E-500 49/84...
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On the home menu the following control buttons are available: Button Meaning Skips the actual step at the selected position. Skips the actual step for all positions. Aborts extraction on the selected extraction position. Editing the running method. 50/84 Operation Manual FatExtractor E-500...
The following parameters can be edited: Device name/DHCP/MAC address/ System IP address/Subnet mask/ Gateway [ Delete app Confirmation question Resets external connections to the connection ] instrument. [ Demo Mode ] On/Off Simulate an extraction. Operation Manual FatExtractor E-500 51/84...
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Explanation [ Extraction unit ] View The following information on the Extraction Unit is available: Serial Number Firmware version Operating hours Temperature electronics Code Line voltage Line frequency 52/84 Operation Manual FatExtractor E-500...
Solvent menu The solvent menu contains the available solvents for the extraction methods. The solvent menu allows editing individual solvents. See Chapter 10 "Editing a sol- vent", page 69 Status bar Shows the actually used solvent and configuration. Operation Manual FatExtractor E-500 53/84...
Setting the solvent of a method The following four solvents are selectable: Chloroform Diethyl ether Hexane Petroleum ether The control sets the heating level for the selected solvent automatically. Navigation path ➔ 54/84 Operation Manual FatExtractor E-500...
Tap the function [ Edit ] on the function bar. Select the action [ Extraction time ] with the navigation control. Turn the navigation control until the required value. Tap the function [ OK ] on the function bar. Operation Manual FatExtractor E-500 55/84...
Setting the Soxhlet valve open time of a method (Soxhlet only) The Soxhlet valve open time depends on the position of the level sensor and the sample structure. Set the Soxhlet valve opening time so that the Extraction glass chamber Soxhlet is completely emptied. 56/84 Operation Manual FatExtractor E-500...
Setting the rinse heating level of a method (Soxhlet and Hot Extraction only) The default setting for the rinse heating level is depending on the selected solvent. Change the rinse heating level to compensate fluctuations in ambient conditions. Operation Manual FatExtractor E-500 57/84...
Smart-Drying automatically sets the parameters for the drying step. The following parameters are set automatically: Rinse heating level Drying time Drying heating level Navigation path ➔ Navigate to the [ Method ] menu via the navigation path. 58/84 Operation Manual FatExtractor E-500...
Select the action [ Drying heating level ] with the navigation control. Turn the navigation control until the required value. Tap the function [ OK ] on the function bar. Tap the function [ Save ] on the function bar. ð The heating level is saved. Operation Manual FatExtractor E-500 59/84...
Put a funnel on the condenser. Add solvent with a beaker. Putting the glass sample tube with frit in the extraction glass chamber NOTE Instead of glass sample tube with frit it is possible to insert a thimble. 60/84 Operation Manual FatExtractor E-500...
R Make sure that the configuration Soxhlet Extraction is selected. R Make sure that there are no extraction beakers in the instrument. R Make sure that no defective sealings or glass parts are used. R Make sure that the tank bottle is empty. Operation Manual FatExtractor E-500 61/84...
R The sample is prepared (in the glass sample tube or in the paper thimbles). Switch on the connected chiller. Pull the chamber rack out of the instrument. Place the prepared glass sample tube or paper thimble with the corresponding holder in the extraction chamber. 62/84 Operation Manual FatExtractor E-500...
9.3.7 Shutting down the instrument Navigation path ➔ Precondition: R The extraction process has ended. Switch the On/Off master switch to Off. Performing a Hot Extraction 9.4.1 Preparing the instrument for a Hot Extraction Precondition: Operation Manual FatExtractor E-500 63/84...
R The sample is prepared (in the glass sample tube or in the paper thimbles). Switch on the connected chiller. Place the prepared glass sample tube or paper thimble with the corresponding holder in the beaker. 64/84 Operation Manual FatExtractor E-500...
R The extraction process has ended. Switch the On/Off master switch to Off. Performing an Economic Continuous Extraction 9.5.1 Preparing the instrument for an Economic Continuous Extraction Precondition: R All commissioning operations have been completed. See Chapter 5 "Installation", page 26 Operation Manual FatExtractor E-500 65/84...
4 Make sure that the solvent is at ambient temperature. Navigation path ➔ Precondition: R The instrument has been prepared. R A method is selected. R The sample is prepared (in the glass sample tube or in the paper thimbles). 66/84 Operation Manual FatExtractor E-500...
Empty the solvent tank. See Chapter 12.2 "Removing the tank bottle", page 73 9.5.6 Shutting down the instrument Navigation path ➔ Precondition: R The extraction process has ended. Switch the On/Off master switch to Off. Exporting extraction parameters 9.6.1 Exporting extraction parameters without cloud connection Precondition: Operation Manual FatExtractor E-500 67/84...
Scan the QR code on the display with the camera function of the App. 9.6.2 Exporting extraction parameters with cloud connection Precondition: R The App is installed on a mobile device. R An extraction is finished. The extraction parameters are automatically transferred to the App. 68/84 Operation Manual FatExtractor E-500...
ð The display shows a dialog with an alphanumeric input box. Enter a name for the solvent. Tap the function [ Save ] on the function bar. ð The new name is saved. ð The dialog box closes. Operation Manual FatExtractor E-500 69/84...
The following port has to be enabled in the firewall settings on the internet gate- way: TCP (HTTPS) traffics through remote port 443 In order to use the BUCHI Cloud a DNS server must be configured on the instru- ment. NOTE If there is no DNS server available enter the IP address for the BUCHI Cloud connec- tion manually.
Mobile connection | 11 11.4 Connecting the instrument and app using a password Navigate to the action [ Mobile connection password ]. ð The display shows a password. Enter the password in the app. Tap [ OK ] Operation Manual FatExtractor E-500 71/84...
Any servicing and repair work which involves opening up the casing may only be car- ried out by BUCHI service technicians. Use only genuine BUCHI consumables and spare parts in order to ensure correct operation of the device and preserve the warranty.
Switch the On/Off master switch to Interface Wipe down the display with a damp Monthly cloth. 12.2 Removing the tank bottle Switch the On/Off master switch to Off. Open the knurled nut at the ball joint clamp. Operation Manual FatExtractor E-500 73/84...
ð The display shows the view Valves. Select the position of the tank valve you wish to open. Open the tank valve. Wait until the all solvent is in the tank bottle. Close the tank valve. 74/84 Operation Manual FatExtractor E-500...
Heater power is set too low Adapt the heating power to the ambient conditions (temperature, level above sea). Choose correct solvent Not enough solvent Add solvent. See Chap- ter 9.1 "Adding solvent through the condenser", page 60 Operation Manual FatExtractor E-500 75/84...
Wait until the extraction glass chamber is empty. Close Soxhlet valve. 13.3 Replacing the gliding elements Positions of the magnet Replacing procedure Attach the back part of the gliding ele- ments to the protection shield. 76/84 Operation Manual FatExtractor E-500...
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BÜCHI Labortechnik AG Help with faults | 13 NOTICE! Insert the magnet at the positions indicated. Insert the magnet. Attach the front part of the gliding ele- ments to the protection shield. Operation Manual FatExtractor E-500 77/84...
When disposing, observe the disposal regulations of the materials used. Materials used see Chapter 3.5 "Technical data", page 22 14.3 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Depart- ment. https://www.buchi.com/contact 78/84 Operation Manual FatExtractor E-500...
Water out Connection Condenser CW OUT Condenser Condenser Flow meter (inside instrument) Condenser Condenser Connection CW Tank OUT Condenser solvent tank CW IN Condenser Connection solvent tank CW Tank IN CW OUT Fig. 16: Condenser circuit Operation Manual FatExtractor E-500 79/84...
Fig. 17: Solvent circuit 15.2 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.
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Allows for turning the instrument for easier access. Set of gliding elements including magnets, 10 11067827 pcs. Soxhlet assembly cpl. 11067065 One part constiting of magnetic valve and level sensor for extraction glass chamber Soxhlet Operation Manual FatExtractor E-500 81/84...
Meant to be used with a vacuum controller/interface. 15.2.2 Conversion kits The FatExtractor E-500 can be converted into another configuration with an easy change of glass assembly. Choose the needed conversion kit according to the matrix. Order no. Conversion kit from Soxhlet Extraction and Economic Continuous Ex-...
Order no. Image Extraction thimbles 25 x 100 mm, 25 pcs. 018105 Extraction thimbles 33 x 94 mm, 25 pcs. 11058983 Extraction thimbles, Set. 25 pcs, 43 x 118 mm, 018106 cellulose For Soxhlet extraction unit. Operation Manual FatExtractor E-500 83/84...
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