Buchi Lyovapor L-300 Operation Manual
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Buchi Lyovapor L-300 Operation Manual

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Lyovapor™ L-300/L-300 Pro
Operation Manual

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Summary of Contents for Buchi Lyovapor L-300

  • Page 1 Lyovapor™ L-300/L-300 Pro Operation Manual...
  • Page 2 CH-9230 Flawil 1 E-Mail: quality@buchi.com BUCHI reserves the right to make changes to the manual as deemed necessary in the light of experience, especially with respect to structure, illustrations and technical details. This manual is copyrighted. Information from it may neither be reproduced, distributed, or used for competitive purposes, nor made available to third parties.

  • Page 3: Table Of Contents

    Büchi Labortechnik AG Table of contents Table of contents About this document...................... 7 Connected devices ........................ 7 Warning notices in this document.................. 7 Symbols.......................... 7 1.3.1 Warning symbols ......................  7 1.3.2 Mark-ups and symbols .....................  8 Trademarks ........................... 8 Safety............................ 9 Proper use .......................... 9 Use other than that intended .................... 9 Staff qualification ........................ 9 Location of warning signs on the product ................
  • Page 4 Requirements for local network settings.............. 35 5.4.2 Preparing the instrument for app using .............. 36 5.4.3 Enabling BUCHI Cloud access................ 36 Insert SD card (Pro control panel only) ................ 36 Operating the control panel.................... 38 Layout of the control panel .................... 38 Function bar......................... 39...
  • Page 5 Büchi Labortechnik AG Table of contents Operating Pro control panel ..................... 52 Layout of Pro control panel.................... 52 Function bar......................... 52 Other symbols on the control panel.................. 54 Menu bar .......................... 54 7.4.1 Start menu ...................... 54 7.4.2 Favourites menu.....................  55 7.4.3 Method menu...................... 55 7.4.4 Configuration menu ....................
  • Page 6 Table of contents Büchi Labortechnik AG Cleaning and servicing ..................... 97 Regular maintenance work.................... 97 Performing a vacuum test.................... 98 Performing a leak test...................... 99 9.3.1 Performing a leak test with a drying chamber ............ 99 9.3.2 Performing leak test with a manifold drying rack .......... 101 Cleaning the ice condenser .................... 103 Help with faults ........................ 104 10.1...

  • Page 7: About This Document

    If you have any questions after reading this operation manual: Contact BÜCHI Labortechnik AG Customer Service. https://www.buchi.com/contact Connected devices In addition to these operating instructions, follow the instructions and specifications in the documentation for the connected devices.

  • Page 8: Mark-Ups And Symbols

    Büchi Labortechnik AG 1 | About this document Symbol Meaning Hot surface Explosive substances Dangerous electrical voltage Flammable substances Instrument damage 1.3.2 Mark-ups and symbols NOTE This symbol draws attention to useful and important information. R This character draws attention to a requirement that must be met before the instructions below are carried out.

  • Page 9: Safety

    Büchi Labortechnik AG Safety | 2 Safety Proper use The Lyovapor™ L-300 is used for freeze-drying solid materials in ampoules, vials, trays, round-neck or wide-neck flasks and is exclusively intended for that purpose. The Lyovapor™ L-300 can be used in laboratories for the following tasks: —...

  • Page 10: Location Of Warning Signs On The Product

    — Safety-related incidents that occur while using the instrument should be reported to the manufacturer (quality@buchi.com). BUCHI service technicians Service technicians authorized by BUCHI have attended special training courses and are authorized by BÜCHI Labortechnik AG to carry out special servicing and repair measures.

  • Page 11: Location Of Warning Signs On The Ice Condenser

    Büchi Labortechnik AG Safety | 2 Fig. 2: Location of warning signs on the product General warning Dangerous electrical voltage Flammable substances Keep at least 40 cm clearance to pre- vent overheating 2.4.3 Location of warning signs on the ice condenser Fig. 3: Location of warning signs on the product Hand injuries Hot surface...

  • Page 12: Residual Risks

    Büchi Labortechnik AG 2 | Safety Residual risks The device has been developed and manufactured using the latest technological advances. Nevertheless, risks to persons, property or the environment can arise if the device is used incorrectly. Appropriate warnings in this manual serve to alert the user to these residual dangers.

  • Page 13: Modifications

    Unauthorized modifications can affect safety and lead to accidents. Use only genuine BUCHI accessories, spare parts and consumables. Carry out technical changes only with prior written approval from BUCHI. Only allow changes to be made by BUCHI service technicians. BUCHI accepts no liability for damage, faults and malfunctions resulting from unauthorized modifications.

  • Page 14: Product Description

    Büchi Labortechnik AG 3 | Product description Product description Description of function The Lyovapor™ is a freeze-dryer in which frozen samples can be gently dried. The basis of freeze-drying is sublimation. Sublimation refers to the process whereby a substance transforms directly from the solid to the gaseous state. The physical process of sublimation can be explained using the solvent water as an example.

  • Page 15: Secondary-Drying Phase

    Büchi Labortechnik AG Product description | 3 If a drying chamber with heating is used, heat transfer takes place additionally by direct contact. The temperature of the heatable shelves is controllable. Control of the thermal energy transferred is then possible. Controlling the heat transfer prevents the following critical temperatures for amorphous and crystalline materials in the solidified sample being reached: —...
  • Page 16 Büchi Labortechnik AG 3 | Product description Fig. 4: Front View Connections for heated shelf Intermediate valve (Lyovapor™ L-300 Pro only) Ice condenser Optional mounting position for con- trol panel Ventilation slots Castors Castor brake On/Off master switch User interface 16/118 Operation Manual Lyovapor™...

  • Page 17: Rear View

    Büchi Labortechnik AG Product description | 3 3.2.2 Rear view Fig. 5: Rear view Power isolating switch Type plate/Refrigerant specifications Connections for pressure sensors Side connections (option) Connection for fresh water Connection for condensate drain hose I Connection for condensate drain Ventilation slots hose II Gas connection for venting valve...

  • Page 18: Side Connections

    Connection for pressure sensor Connection for pressure sensor (option) (option) Automatic stoppering connection Connection for fresh water sensor Connection for receiving vessel sen- Connection for external pressure sensors (optional) LAN port Standard BUCHI communication port (COM) 18/118 Operation Manual Lyovapor™ L-300/L-300 Pro...

  • Page 19: Control Panel

    Büchi Labortechnik AG Product description | 3 3.2.4 Control panel Fig. 7: Control panel View Function buttons Navigation control 3.2.5 Pro control panel Fig. 8: Pro control panel Touch-screen display Navigation control Type plate The type plate identifies the instrument. The type plate is located at the rear of the instrument.

  • Page 20: Scope Of Delivery

    Büchi Labortechnik AG 3 | Product description Buchi Operations India Private Limited 394230 Surat / India Type xxxxxxxx Volt xxx-xxx Freq Power xxxx Built 20xx Designed by BUCHI in Switzerland Made in India Fig. 9: Type Plate Company name and address...

  • Page 21: Refrigerant Specification (High Temperature)

    Büchi Labortechnik AG Product description | 3 L-xxx xxHz LT Cooling System Refrigerant: Rxxx Filling: xxx g Global Warming Potential (GWP): xx tCO High Side Design Pressure: xxxx Low Side Design Pressure: xxxx Fig. 10: Refrigerant details Instrument name Refrigerant details Filling capacity Global warming potential High-pressure system design pres-...

  • Page 22: Lyovapor™ L-300

    Büchi Labortechnik AG 3 | Product description Specification L-300 for 50Hz L-300 for 60Hz Electrical connector CEE 400 V 16 A (IEC NEMA L21-20, 4- 60309),3P+N+PE , 6h, pole/5-wire, 20 A, 3Ø (Specified according to UK standard) 208 V Max. current for all connections 0.5 A 0.5 A Voltage per connection...

  • Page 23: Ambient Conditions

    Büchi Labortechnik AG Product description | 3 Specification L-300 for 50Hz L-300 for 60Hz Volume-based leakage rate* Typically ≤ 0.001 mbar Typically ≤ 0.001 mbar x L/sec x L/sec Minimum system vacuum Typically ≤ 30 mTorr Typically ≤ 30 mTorr (with default vacuum pump / with- out samples) Vacuum control range...
  • Page 24 Büchi Labortechnik AG 3 | Product description Component Materials of construction Condensate drain valve Steel 1.4301/304 with EPDM sealings Sili- con drain tubing Venting valve Brass with FKM seal Regulating valve EN 1.4301 Silicone Steam generation EN 1.4301 EPDM seal Silicone 24/118 Operation Manual Lyovapor™...

  • Page 25: Transport And Storage

    Büchi Labortechnik AG Transport and storage | 4 Transport and storage Transport NOTICE Risk of breakage due to incorrect transportation Make sure that the instrument is fully dismantled. Pack every instrument components properly to prevent breakage. Use the original packaging whenever possible. Avoid sharp movements during transit.

  • Page 26: Installation

    Büchi Labortechnik AG 5 | Installation Installation Installation site The installation site must meet the following requirements: DANGER Risk of explosion from flammable gas-and-air mixtures The potential consequences are death or extremely serious injury. Do not damage the refrigerant circuit piping. Store and operate the instrument in a room with a volume of at least 16.6 m that an ignitable gas-and-air mixture cannot develop.

  • Page 27: Putting The Instrument In Operation

    Büchi Labortechnik AG Installation | 5 Fig. 12: Castor brake NOTE Make sure that the power supply can be disconnected at any time in an emergency. NOTE Depending on the ambient conditions, condensation may form on any cold surface on the unit. Putting the instrument in operation NOTICE Instrument damaged if restarted too early...

  • Page 28: Fitting The Fresh Water Connections

    Büchi Labortechnik AG 5 | Installation 2. Be designed for the load imposed by the instruments connected. 3. Be equipped with suitable fuses and electrical safety features. 4. Be equipped with a proper earthing. Make sure that all connected devices are earthed. Make sure that the power plug is freely accessible at all times.

  • Page 29: Fitting Fill-Level Sensor In Water Tank (Optional)

    Büchi Labortechnik AG Installation | 5 R Depending on the volume of the sample and the environmental conditions 3 L to 8.5 L water in 24 h is necessary. Switch the On/Off master switch to Off. Fit the inlet tube (4) onto the connection marked Fresh Water (5). Fit the fresh water level sensor (optional).

  • Page 30: Fitting The Fill-Level Sensor On The Receiving Vessel (Optional)

    Büchi Labortechnik AG 5 | Installation Fig. 15: Fitting the drain tube Side connections Drain tube See Chapter 3.2.3 "Side connec- tions", page 18 Connection for condensate drain tube Switch the On/Off master switch to Off. Fit the drain tube (4) onto the connection marked Drain 1 (5). Fix the drain tube in place with a hose clip.

  • Page 31: Connecting The Coolant

    Büchi Labortechnik AG Installation | 5 Plug the receiving vessel sensor electrical connection into the socket marked Waste Water. 5.2.7 Connecting the coolant Fig. 17: Connecting the coolant Coolant inlet connection Coolant outlet connection Drain tube Inlet tube Precondition: R The coolant connection complies with the specified parameters. See Chapter 3.6 "Technical data", page 21 Make sure that the instrument is not connected to the power supply.
  • Page 32 Büchi Labortechnik AG 5 | Installation Fig. 18: Fitting the pressure sensors Pressure sensor Pressure sensor Clamp, ISO-KF 16 Clamp, ISO-KF 16 Seal, ISO-KF 16 Connection for pressure sensor Seal, ISO-KF 16 Connection for pressure sensor Connection 10 Connection Fitting pressure sensor one Switch the On/Off master switch to Off.

  • Page 33: Connecting Inert Gas (Option)

    Büchi Labortechnik AG Installation | 5 5.2.9 Connecting inert gas (option) Fig. 19: Connecting inert gas Inert gas tube Tube clip Gas connection for pressure regulat- Gas connection for venting valve ing valve Tube clip Inert gas tube Connecting the inert gas to the pressure regulating valve Precondition: R The gas connection complies with the specified parameters.

  • Page 34: Fitting An Air Filter (Option)

    Büchi Labortechnik AG 5 | Installation 5.2.10 Fitting an air filter (option) Fig. 20: Fitting an air filter Air filter Tube clip Tubing Tube clip Gas connection for pressure regulat- Gas connection for venting valve ing valve Tube clip Tubing Tube clip 10 Air filter Fitting an air filter for the pressure regulating valve Precondition:...

  • Page 35: Commissioning The Vacuum Pump

    Büchi Labortechnik AG Installation | 5 Commissioning the vacuum pump The vacuum pump evacuates the top-mount drying rack during the freeze-drying process. NOTICE Open gas ballast valve. A closed gas ballast valve when using solvents can cause damage to the instrument. Open the gas ballast valve.

  • Page 36: Preparing The Instrument For App Using

    Büchi Labortechnik AG 5 | Installation In order to use the BUCHI Cloud a DNS server must be configured on the instrument. NOTE If there is no DNS server available enter the IP address for the BUCHI Cloud connection manually.
  • Page 37 Büchi Labortechnik AG Installation | 5 Fold the control panel forward. Insert the SD card on the underside. Switch on the instrument. ð The status bar shows the SD card symbol. The following data is stored on the SD card: —...

  • Page 38: Operating The Control Panel

    Büchi Labortechnik AG 6 | Operating the control panel Operating the control panel This section describes the operation of the instrument using the control panel. CAUTION Risk of injury from glass splinters Sharp objects can damage the display. Keep sharp objects away from the display. NOTICE Unnecessary operation hours can affect the lifetime of the instrument.

  • Page 39: Function Bar

    Büchi Labortechnik AG Operating the control panel | 6 Function bar The function bar shows available functions according to the current operation. The functions on the function bar are executed by tapping the relevant function buttons or pressing the navigation control. General function buttons Symbol Description...

  • Page 40: Start Menu

    Büchi Labortechnik AG 6 | Operating the control panel Menu Meaning Submenu/Action symbol [Start] menu — Process control parameters — Bookmarks for individual entry points [Favourites] menu — Process settings [Configuration] menu — Settings — Servicing — Service — System information —...
  • Page 41 Action Option Explanation [Mobile connection View The control panel shows a password password] for entry in the BUCHI Monitor app. [Mobile connection View The control unit shows a QR code for QR code] reading by the BUCHI Monitor app. [Language]...
  • Page 42 Display illumination level in %: 0 - [Network] Enter value The following parameters can be edited: Device name/MAC address/DHCP/ System IP address/Subnet mask/ Gateway/DNS server/BUCHI Cloud/ Server IP address [Delete app connec- Confirmation question Resets external connections to the tion] instrument.
  • Page 43 Büchi Labortechnik AG Operating the control panel | 6 Action Option Explanation [Refrigerant circuit] View The following information on the re- frigerant circuit is available: — Operating hours — Compressor high temperature — Compressor low temperature — Inlet temperature, ice condenser 1 —...
  • Page 44 Büchi Labortechnik AG 6 | Operating the control panel Action Option Explanation [Vacuum system] View The following information on the vacuum system is available: — Operating hours pump — Pump oil hours of duty — Pressure, ice condenser 1 — Pressure, ice condenser 2 —...
  • Page 45 Büchi Labortechnik AG Operating the control panel | 6 Action Option Explanation [Defrosting system] View The following information on the de- frosting system is available: — Duty hours — Steam generator — Water pump — Drain valve 1 — Drain valve 2 —...

  • Page 46: Messages Menu

    Büchi Labortechnik AG 6 | Operating the control panel Action Option Explanation [Instrument] View The following information is avail- able: — Serial number — Firmware version — Hours of duty — Status — Board Temperature — 48V power supply — 24V power supply —...

  • Page 47: Status Bar

    (> 15 min). The current parameters of the freeze-drying process are being re-established. Symbols on the status bar Symbol Status The instrument is connected to the BUCHI Cloud. The instrument is defrosting. Operation Manual Lyovapor™ L-300/L-300 Pro 47/118...

  • Page 48: Carrying Out Freeze-Drying

    Büchi Labortechnik AG 6 | Operating the control panel Symbol Status The instrument is starting up. The instrument is in energy-saving mode. Before the freeze-drying process: Load the top-mount drying rack with a frozen sample. After the freeze-drying process: Remove the dried sample from the top-mount drying rack.

  • Page 49: Starting Freeze-Drying

    Büchi Labortechnik AG Operating the control panel | 6 Tap the function [[Start conditioning]] on the function bar. ð The temperature in the ice condenser cools down to operating temperature. ð The vacuum pump is being brought up to operating temperature. ð...

  • Page 50: Ending Freeze-Drying

    Büchi Labortechnik AG 6 | Operating the control panel 6.5.4 Ending freeze-drying Navigation path ➔ Start Precondition: R The sample is dry. Navigate to the [Start] menu via the navigation path. Tap the function [Aerate] on the function bar. Answer YES to the confirmation question. ð...

  • Page 51: Switching Off The Instrument

    Büchi Labortechnik AG Operating the control panel | 6 6.5.6 Switching off the instrument Precondition: R The instrument has been shut down. See Chapter 6.5.5 "Shutting down the instrument", page 50 Switch the On/Off master switch to Off. Operation Manual Lyovapor™ L-300/L-300 Pro 51/118...

  • Page 52: Operating Pro Control Panel

    Büchi Labortechnik AG 7 | Operating Pro control panel Operating Pro control panel This section describes the operation of the instrument using the Pro control panel. CAUTION Risk of injury from glass splinters Sharp objects can damage the display. Keep sharp objects away from the display. NOTICE Unnecessary operation hours can affect the lifetime of the instrument.
  • Page 53 Büchi Labortechnik AG Operating Pro control panel | 7 The functions on the function bar are executed by tapping the relevant function buttons or pressing the navigation control. General function buttons Symbol Description Meaning [Back] The display reverts to the previous view.

  • Page 54: Other Symbols On The Control Panel

    Büchi Labortechnik AG 7 | Operating Pro control panel Symbol Description Meaning [[Deactivate sample pro- Manually deactivates sample protec- UNPROT tection]] tion. COPY [Copy] Copies the selected method. OPEN [Open] Opens the selected valve. CLOSE [Close] Closes the selected valve. Other symbols on the control panel Symbol Description...

  • Page 55: Favourites Menu

    Büchi Labortechnik AG Operating Pro control panel | 7 Tap the function [Edit] on the function bar. ð The control panel highlights the selected parameter in black. To increase or decrease the figure, use the dialog box with a numeric input. Press the navigation control.
  • Page 56 Action Option Explanation [Mobile connection View The control panel shows a password password] for entry in the BUCHI Monitor app. [Mobile connection View The control unit shows a QR code for QR code] reading by the BUCHI Monitor app. [Language]...
  • Page 57 Display illumination level in %: 0 - [Network] Enter value The following parameters can be edited: Device name/MAC address/DHCP/ System IP address/Subnet mask/ Gateway/DNS server/BUCHI Cloud/ Server IP address [Delete app connec- Confirmation question Resets external connections to the tion] instrument.
  • Page 58 Büchi Labortechnik AG 7 | Operating Pro control panel Action Option Explanation [Refrigerant circuit] View The following information on the re- frigerant circuit is available: — Operating hours — Compressor high temperature — Compressor low temperature — Inlet temperature, ice condenser 1 —...
  • Page 59 Büchi Labortechnik AG Operating Pro control panel | 7 Action Option Explanation [Vacuum system] View The following information on the vacuum system is available: — Operating hours pump — Pump oil hours of duty — Pressure, ice condenser 1 — Pressure, ice condenser 2 —...
  • Page 60 Büchi Labortechnik AG 7 | Operating Pro control panel Action Option Explanation [Defrosting system] View The following information on the de- frosting system is available: — Duty hours — Steam generator — Water pump — Drain valve 1 — Drain valve 2 —...

  • Page 61: Messages Menu

    Büchi Labortechnik AG Operating Pro control panel | 7 Action Option Explanation [Instrument] View The following information is avail- able: — Serial number — Firmware version — Hours of duty — Status — Board Temperature — 48V power supply — 24V power supply —...

  • Page 62: Status Bar

    Büchi Labortechnik AG 7 | Operating Pro control panel Symbol Description Meaning > Received The display shows a message. Status bar The status bar shows the status of the instrument. The following statuses are possible: Indication on status bar View Status Unload / Load Conditioning is completed.
  • Page 63 The instrument is starting up. The instrument is in the course of a manual freeze-drying process. The instrument is connected to the BUCHI Cloud. Sample protection is active. Reason: the pressure is outside the pressure lim- its.

  • Page 64: Editing A Method

    Büchi Labortechnik AG 7 | Operating Pro control panel Symbol Status The ice condenser is white: — Attach the sample. The ice condenser is yellow: — The ice condenser is preparing for switch over. — Sample attachment is possible as long p and T settings are stable.

  • Page 65: Setting The Sample Collapse Temperature

    Büchi Labortechnik AG Operating Pro control panel | 7 Tap the setting [Name]. ð The control panel shows a blank box with an alphanumeric input box. Enter a name for the method. Tap the function [Save] on the function bar. ð...

  • Page 66: Setting The Steps Of A Method

    Büchi Labortechnik AG 7 | Operating Pro control panel Tap the name of the method that you wish to edit. ð The control panel highlights the selected method in green. Tap the action [General]. ð The control panel shows the action [General]. Tap the action [Shelf load temp.] ð...

  • Page 67: Setting The Phases Of A Method

    Büchi Labortechnik AG Operating Pro control panel | 7 Setting Option Meaning [Pressure limit] Enter value Absolute value for divergence from the set pressure before the sample protection function is ac- tivated. [Pressure duration] Enter value Sets the period of time that the pressure is allowed to exceed the pressure limit before the sample protection function is ac-...
  • Page 68 Büchi Labortechnik AG 7 | Operating Pro control panel Phase Setting Option Meaning [Primary [Pressure ac- None/Sample None: no action is carried out. drying] tion] protection/ Sample protection: if the pressure is Message too high, heating of the shelves is paused.

  • Page 69: Deleting A Method

    Büchi Labortechnik AG Operating Pro control panel | 7 Phase Setting Option Meaning [Stopper- [Pressure Regulated/Mini- Regulated: the settings for pressure ing] range] and pressure limits are applied. Minimum: the lowest possible vac- uum pressure is applied. [Pressure] Enter value Sets a target value for the regulated pressure.

  • Page 70: Pressure Difference Test

    Büchi Labortechnik AG 7 | Operating Pro control panel It is possible to use a single test or combine these tests. It is possible to use the tests for automatic transition to the next phase. Settings: [Continue] action setting Explanation The test is used for automatic transition.

  • Page 71: Temperature Difference Test

    Büchi Labortechnik AG Operating Pro control panel | 7 Setting Option Explanation [[Pressure dif- Enter value Specifies the difference between ference limit]] the two sensor readings below which the end point is reached. [[Duration]] Enter value The period of time during which the difference is not to be ex- ceeded.

  • Page 72: Pressure Rise Test

    Büchi Labortechnik AG 7 | Operating Pro control panel Tap the setting [End point definition]. ð The control panel shows the setting End point definition. Tap [Temperature difference test]. ð The control panel shows the temperature difference test. The following settings are available: Setting Option Explanation...
  • Page 73 Büchi Labortechnik AG Operating Pro control panel | 7 Navigate to the [Method] menu via the navigation path. Tap the name of the method that you wish to edit. ð The control panel highlights the selected method in green. Tap the action [Phase]. ð...

  • Page 74: Performing Freeze-Drying Using A Method [Pro Control Panel]

    Büchi Labortechnik AG 7 | Operating Pro control panel Setting Option Explanation [Continue] Yes/No Yes : the method switches to the next phase. No: the phase is ended when the set levels are reached. [Message] Yes/No The control panel shows or does not show a message as soon as the temperature difference test is passed.

  • Page 75: Starting Freeze-Drying

    Büchi Labortechnik AG Operating Pro control panel | 7 7.9.3 Starting freeze-drying CAUTION Risk of skin burns from touching parts of the ice condenser after completion of conditioning. Wear protective gloves when working on the instrument after the conditioning phase. NOTE The freeze-drying process can be cancelled by tapping the functions [Manual] and [Aerate] on the [Start] menu.

  • Page 76: Changing Parameters While The Process Is Running

    Büchi Labortechnik AG 7 | Operating Pro control panel 7.9.4 Changing parameters while the process is running NOTE Coming step can be deleted. Select the step you wish to delete. Tap the [Delete] button on the function bar. 7.9.5 Ending freeze-drying Navigation path ➔...

  • Page 77: Switching Off The Instrument

    Büchi Labortechnik AG Operating Pro control panel | 7 Tap the function [Shut down] on the function bar. ð The instrument is shutting down. ð The status bar shows the remaining time and the status Shutting down. ð After the instrument has shut down, the status bar shows the remaining time and the status Defrosting.

  • Page 78: 7.10.3 Editing Parameters While The Process Is Running

    Büchi Labortechnik AG 7 | Operating Pro control panel Navigation path ➔ Start Precondition: R The instrument has been prepared. Fit a top-mount drying rack. See Chapter 8 "Operating top-mount drying racks", page 81. Load the top-mount drying rack with frozen samples. Navigate to the [Start] menu via the navigation path.

  • Page 79: 7.10.5 Ending Freeze-Drying

    Büchi Labortechnik AG Operating Pro control panel | 7 Navigation path ➔ Configuration ➔ End point determination ➔ Pressure rise test Precondition: R The process has been started. Navigate to the action [Pressure rise test] via the navigation path. On the line [Pressure limit] enter the pressure increase (delta p) within the running test.

  • Page 80: 7.10.7 Switching Off The Instrument

    Büchi Labortechnik AG 7 | Operating Pro control panel Navigation path ➔ Start Precondition: R The freeze-drying process has ended. R The gas ballast on the vacuum pump is open. R Make sure that there is sufficient distilled water available. Navigate to the [Start] menu via the navigation path.

  • Page 81: Operating Top-Mount Drying Racks

    Büchi Labortechnik AG Operating top-mount drying racks | 8 Operating top-mount drying racks Operating stoppering acrylic drying chamber Make sure that the groove above the ice condenser is clean, free of dust and not scratched. Check the O-ring for damage. Place the O-ring into the groove on the ice condenser.
  • Page 82 Büchi Labortechnik AG 8 | Operating top-mount drying racks Place the intermediate plate on the main plate. Loosen all fixing screws. Align the slots for the shelves. Tighten the fixing screws. Place the rack on the intermediate plate. 82/118 Operation Manual Lyovapor™ L-300/L-300 Pro...
  • Page 83 Büchi Labortechnik AG Operating top-mount drying racks | 8 Slide the shelves into the rack. Press the plug onto the shelf connection and turn the ring counterclockwise at the same time. (option) Turn the temperature sensor electrical connector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical connector onto the connection.

  • Page 84: Operating Manifold Acrylic Drying Chamber (Heatable Shelves)

    Büchi Labortechnik AG 8 | Operating top-mount drying racks Make sure that the hooks on the rack are engaged. Carry out freeze-drying. Precondition: R The status bar shows the status Stoppering. Turn the hand wheel, until all samples are sealed. Confirm the verification question on the control panel.
  • Page 85 Büchi Labortechnik AG Operating top-mount drying racks | 8 Make sure that the groove on the ice condenser is clean, free of dust and not scratched. Check the 300 mm dia. O-ring for damage. Place the 300mm dia. O-ring in the groove in the main plate.
  • Page 86 Büchi Labortechnik AG 8 | Operating top-mount drying racks Place the rack on the intermediate plate. Slide the shelves into the rack. Press the plug onto the shelf connection and turn the ring counterclockwise at the same time. (option) Turn the temperature sensor electrical connector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical...

  • Page 87: Operating Manifold Acrylic Drying Chamber (Non-Heatable Shelf)

    Büchi Labortechnik AG Operating top-mount drying racks | 8 Fit manifold valve onto the connection on the top-mount drying rack. Check the O-ring for damage. Place the O-ring into the groove of the cover. Place the manifold cover on the cylinder. Carry out freeze-drying.
  • Page 88 Büchi Labortechnik AG 8 | Operating top-mount drying racks Place the main plate on the ice condenser. Make sure that the groove on the ice condenser is clean, free of dust and not scratched. Check the 300 mm dia. O-ring for damage. Place the 300mm dia.
  • Page 89 Büchi Labortechnik AG Operating top-mount drying racks | 8 Locate the cylinder in the groove above the main plate. Fit manifold valve onto the connection on the top-mount drying rack. Check the O-ring for damage. Place the O-ring into the groove of the cover. Place the manifold cover on the cylinder.

  • Page 90: Operating Acrylic Drying Chamber (Heatable Shelf)

    Büchi Labortechnik AG 8 | Operating top-mount drying racks Operating acrylic drying chamber (heatable shelf) Make sure that the groove above the ice condenser is clean, free of dust and not scratched. Check the O-ring for damage. Place the O-ring into the groove on the ice condenser.
  • Page 91 Büchi Labortechnik AG Operating top-mount drying racks | 8 Align the slots for the shelves. Tighten the fixing screws. Place the rack on the intermediate plate. Slide the shelves into the rack. Press the plug onto the shelf connection and turn the ring counterclockwise at the same time.

  • Page 92: Operating Acrylic Drying Chamber (Non-Heatable Shelf)

    Büchi Labortechnik AG 8 | Operating top-mount drying racks (option) Turn the temperature sensor electrical connector so that the marks on the connector and the heatable shelf are parallel Press the temperature sensor electrical connector onto the connection. Locate the cylinder in the groove above the main plate.
  • Page 93 Büchi Labortechnik AG Operating top-mount drying racks | 8 Make sure that the groove on the ice condenser is clean, free of dust and not scratched. Check the 300 mm dia. O-ring for damage. Place the 300mm dia. O-ring in the groove in the main plate.

  • Page 94: Operating Manifold Drying Rack

    Büchi Labortechnik AG 8 | Operating top-mount drying racks Check the O-ring for damage. Place the O-ring into the groove of the cover. Place the cover on the cylinder. Carry out freeze-drying. Operating manifold drying rack Make sure that the groove above the ice condenser is clean, free of dust and not scratched.
  • Page 95 Büchi Labortechnik AG Operating top-mount drying racks | 8 Fit manifold valve onto the connection on the top-mount drying rack. Place the base plate on the main plate. Place the seal on the connection. Fit the manifold drying rack onto the seal and fix it with the clamp.

  • Page 96: Determining The Number Of Flasks

    Büchi Labortechnik AG 8 | Operating top-mount drying racks Determining the number of flasks NOTE Do not overfill flasks Maximum volume of the samples ≤ half of the flask volume NOTE The number of flasks used should be determined individually for every process. The number of flasks used is dependent on the following factors: —...

  • Page 97: Cleaning And Servicing

    NOTE Users may only carry out the servicing and cleaning operations described in this section. Any servicing and repair work which involves opening up the casing may only be carried out by BUCHI service technicians. Regular maintenance work Component Action...

  • Page 98: Performing A Vacuum Test

    Büchi Labortechnik AG 9 | Cleaning and servicing Component Action Interval Heat exchanger Remove dust and foreign objects from Monthly the ventilation slots using compressed Heater air or a vacuum cleaner. View Wipe down the display with a damp Monthly cloth.

  • Page 99: Performing A Leak Test

    Büchi Labortechnik AG Cleaning and servicing | 9 Possible cause Action Top-mount drying rack is damaged Check function of PMMA parts, replace manifold valves, clean drain valve. O-rings dirty Wipe down the O-rings with a damp cloth. O-rings damaged Check O-rings and replace if necessary. Groove of O-rings dirty Wipe groove of O-rings with a damp cloth.
  • Page 100 Büchi Labortechnik AG 9 | Cleaning and servicing Ice condenser 1 24.4L 47.2L 54.1L Acrylic drying chamber Acrylic drying chamber (with 4 drying shelves) (with 6 drying shelves) Ice condenser 2 24.4L 47.2L 54.1L Acrylic drying chamber Acrylic drying chamber (with 4 drying shelves) (with 6 drying shelves) Both ice condensers...

  • Page 101: Performing Leak Test With A Manifold Drying Rack

    Büchi Labortechnik AG Cleaning and servicing | 9 ð The leak test is passed if the measured leakage rate is less than the pre-set rate of 10.10 mbar*L/h. Troubleshooting after failed leak test Possible cause Action Top-mount drying rack not correctly fit- Fit the top-mount drying rack correctly.
  • Page 102 Büchi Labortechnik AG 9 | Cleaning and servicing 24.4L 24.4L 33.5L Tap the function [Start] on the function bar. ð The leak test starts. ð The status bar shows the status Leak Test. ð When the leak test is completed, the [Leak test] tab indicates whether the leak test was successful.

  • Page 103: Cleaning The Ice Condenser

    Büchi Labortechnik AG Cleaning and servicing | 9 Cleaning the ice condenser NOTICE Do not use force to remove the ice from the ice condenser. Opening intermediate valve Navigation path ➔ Configuration ➔ Service ➔ Ice condenser Precondition: R The instrument is in standby mode. See Chapter 7.9.6 "Shutting down the instrument", page 76 Navigate to the view via the navigation path.

  • Page 104: Help With Faults

    Clean the filter at pump outlet in a an ultrasonic bath. Drain valve is leaking. Check whether closed drain tube contracts. If it does, contact BUCHI Service. 104/118 Operation Manual Lyovapor™ L-300/L-300 Pro...

  • Page 105: Locating Leaking Manifold Valve

    Büchi Labortechnik AG Help with faults | 10 Malfunction Possible cause Remedy Instrument is not The connection for the gas Make sure that gas is able to vented supply is blocked. flow into the instrument through the gas supply connection. Clean the air filter (option).

  • Page 106: Error Messages

    Büchi Labortechnik AG 10 | Help with faults Defrosting the ice condenser Navigation path ➔ Configuration ➔ Service ➔ Defrosting system Precondition: R A receiving vessel is installed. See Chapter 5.2.5 "Fitting the drain tubes", page 29 Pour approx. 5 L of warm water into the ice condenser through the open intermediate valve.
  • Page 107 Büchi Labortechnik AG Help with faults | 10 Error Possible cause Solution message 5041 Only Pro instrument. Choose lower shelf set temperature. The actual pressure in ice con- Resolve any leakages. denser is above defined safety pressure during a method run. 5071 Motor driver of intermediate Aerate the drying chamber by...
  • Page 108 Büchi Labortechnik AG 10 | Help with faults Error Possible cause Solution message 5275 Pressure of 500 mbar cannot be Resolve any leaks. reached within 30s. 5278 Pressure increases above level of Resolve any leaks. 1.5 mbar during leak test. Clean and dry condenser chamber.
  • Page 109 5570 Heatable shelf is disconnected. Connect a functioning shelf at the same connector position. Defective component (e.g. heat- Contact BUCHI Customer Service. ing coil, cable, plug). 5571 Sample temperature sensor is Connect a functioning PT-1000 at disconnected. the same connector position.

  • Page 110: Taking Out Of Service And Disposal

    Dispose of the appliance properly, if necessary using a professional disposal service. When disposing of equipment observe the local regulations and statutory requirements regarding waste disposal. 11.2 Returning the instrument Before returning the instrument, contact the BÜCHI Labortechnik AG Service Department. https://www.buchi.com/contact 110/118 Operation Manual Lyovapor™ L-300/L-300 Pro...

  • Page 111: Appendix

    Appendix 12.1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct, safe and reliable operation of the system. NOTE Any modifications of spare parts or assemblies are only allowed with the prior written permission of BUCHI.

  • Page 112: 12.1.3 Spare Parts

    Büchi Labortechnik AG 12 | Appendix Order no. Image SD-Card 1 GB 11064730 Connection cable, for vacuum pump 11064934 12.1.3 Spare parts Description Order no. Image User interface 11063580 User interface Pro 11063581 O-Ring Ø 300 mm 11065367 O-Ring Ø 430 mm 11065343 Vacuum hose, KF 25, L 1000 mm 11066031...
  • Page 113 Büchi Labortechnik AG Appendix | 12 Description Order no. Image Vacuum seal KF 16 11063455 Vacuum seal KF 25 11063457 Vacuum seal KF 40 11063659 O-Ring, Silicone, 94.61 x 6.99 mm 11062420 O-Ring, Silicone, 161.3 x 5.33 mm 11062421 Edward vacuum pump outlet filter 11068459 Blind flange KF 16 11064902...

  • Page 114: 12.1.4 Top-Mount Drying Rack Accessories

    Büchi Labortechnik AG 12 | Appendix Description Order no. Image Blind flange KF 40 11063661 12.1.4 Top-mount drying rack accessories Order no. Image Drying chamber tube, PMMA, for 4 shelves 11063278 L 368 mm, Ø 300 mm Drying chamber tube, PMMA, for 6 shelves 11065093 L 480 mm, Ø...
  • Page 115 Büchi Labortechnik AG Appendix | 12 Order no. Image Ferrule, 11065816 Ø 218 mm, H 40 mm Drying rack manifold, stainless steel, with 12 11063664 valves H 340 mm, W 777 mm Drip pan, for manifold 11066358 Support for rack, stainless steel 11063789 H 4 mm Baseplate, stainless steel, for manifold rack...

  • Page 116: 12.1.5 Software

    Büchi Labortechnik AG 12 | Appendix Order no. Image Flask beaker for manifold 1200 mL 11066143 with cap adapter and integrated filter Manifold flask adapter set 11066144 with 12 adapters, incl. filter paper Manifold flask adapter set 11067334 with 6 adapters, incl. filter paper Manifold flask adapter set, US joint size 11066171 with 12 adapters, incl.
  • Page 118 We are represented by more than 100 distribution partners worldwide. Find your local representative at: www.buchi.com...

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