Related Manuals for Bruker minispec LF Series
Summary of Contents for Bruker minispec LF Series
- Page 1 minispec ● minispec LF-series User Manual Version 002 Innovation with Integrity...
- Page 2 Product names used are trademarks or registered trademarks of their re- spective holders. © May 16, 2019 Bruker BioSpin GmbH Document Number: 10000058222 P/N: E164809...
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Page 3: Table Of Contents
Contents Contents Introduction............................ 5 Policy Statement ......................... 7 Safety.............................. 9 Intended Use........................ 9 Qualified Personnel...................... 9 Correct Usage........................ 9 General Workplace Dangers..................... 10 2.4.1 Lifting Safety ........................ 10 2.4.2 Safety When Working with Glassware................ 10 Dangers from Electrical Power .................. 11 Dangers from Magnetic Fields .................. 12 2.6.1 Example Stray Field Plots.................... 12 Transport, Packaging and Storage .................... 15 Symbols on the Packaging .................... 15 Inspection at Delivery...................... 16... - Page 4 Contents 6.2.4.6 The Parameter Menu ...................... 37 6.2.4.7 The View Menu ......................... 40 6.2.4.8 The Process Menu...................... 44 6.2.4.9 The Window Menu ...................... 45 6.2.4.10 The Help Menu ......................... 46 Running minispec Plus Software .................. 47 6.3.1 Daily Check........................ 48 General Measurement Handling .................. 49 Basic NMR Principles........................ 51 NMR Parameters.......................... 53 Scans .......................... 53 Recycle Delay Time ...................... 53...
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Page 5: Introduction
LF-series is also used for a diverse range of applications in research and development (R&D) laboratories in industry, universities, and medical research facilities. This manual provides instructions on the initial installation of the instrument by Bruker Service or personnel authorized by Bruker, including: •... - Page 6 The figures shown in this manual are designed to be general and informative and may not represent the specific Bruker model, component or software/firmware version you are working with. Options and accessories may or may not be illustrated in each figure.
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Page 7: Policy Statement
Introduction Policy Statement It is Bruker’s policy to improve products as new techniques and components become available. Bruker reserves the right to change specifications at any time. Every effort has been made to avoid errors in text and figure presentation in this publication. - Page 8 Introduction 8 / 72 E164809_2_002...
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Page 9: Safety
Correct Usage This device and its components may only be used in the way discussed with a Bruker specialist and in connection with devices or components from other manufacturers which have been approved or recommended by Bruker. -
Page 10: General Workplace Dangers
Safety General Workplace Dangers 2.4.1 Lifting Safety The standard magnet unit may weigh up to 150 kg depending on the type of analyzer, to ensure safety the follow safety instructions must be followed: CAUTION Risk of injury from lifting heavy objects. The unit is very heavy and may lead to injury when lifting. -
Page 11: Dangers From Electrical Power
No other components within the spectrometer should be touched (even with the power off) without first contacting a Bruker service representative to obtain all necessary instructions, to ensure user and spectrometer safety. E164809_2_002... -
Page 12: Dangers From Magnetic Fields
Safety Dangers from Magnetic Fields The instrument contains strong magnetic fields, some of which extend beyond the magnet unit house (see stray field diagram below). These pose no danger to routine users, but are hazardous to persons with heart pacemakers or other medical implantations. WARNING Risk to life due to high magnetic fields A magnetic field of more than 0.5 mT (5 Gauss) is life-threatening for people with... -
Page 13: Figure 2.2: Example Of A Stray Field Plot For The Lf90 - Top View
Safety Figure 2.2: Example of a Stray Field Plot for the LF90 - Top View E164809_2_002 13 / 72... - Page 14 Safety 14 / 72 E164809_2_002...
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Page 15: Transport, Packaging And Storage
Installation, initial commissioning, retrofitting, repairs, adjustments or dismantling of the device must only be carried out by Bruker Service or personnel authorized by Bruker. Damage due to servicing that is not authorized by Bruker is not covered by your warranty. Symbols on the Packaging The following symbols are affixed to the packaging material. -
Page 16: Inspection At Delivery
Transport, Packaging and Storage Do not Damage Air-tight The packaging is air-tight. Damage to the Packaging barrier layer may render the contents unusable. Do not pierce. Do not use sharp objects to open. Component Sensitive to The packaging contains components which Electrostatic Charge are sensitive to an electrostatic charge. -
Page 17: Storage
Transport, Packaging and Storage It is recommended to store the packaging for later use in the event that you need to return the unit to Bruker. Handling Packaging Materials Dispose of packaging material in accordance with the relevant applicable legal requirements... - Page 18 Transport, Packaging and Storage 18 / 72 E164809_2_002...
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Page 19: Design And Function
Design and Function Design and Function The minispec LF-series is a benchtop system for time-domain nuclear magnetic resonance (TD-NMR) measurements primarily assigned for quality control. The system consists of a magnet and an electronic unit, a PC workstation, the user software and optionally a pulsed gradient unit, automation, a printer, a balance or a bar-code reader. -
Page 20: Movable Working Table (Cart)
– or so-called cart. The cart is standard with the shipment of all minispec LF-systems. As special feature: There is a larger cart upgrade available for minispec LF90II and LF110. During the installation the Bruker Service representative will assemble this cart. 20 / 72 E164809_2_002... -
Page 21: Installation
Installation, initial commissioning, retrofitting, repairs, adjustments or dismantling of the device must only be carried out by Bruker Service or personnel authorized by Bruker. Damage due to servicing that is not authorized by Bruker is not covered by your warranty. Siting Considerations... - Page 22 Installation Care should be taken to avoid the following hostile influences: • Direct sunlight. • Air draughts. • Rapidly changing room temperature. • Vibration or mechanical shock. • Strong static magnetic fields (i.e. close to other NMR instruments). • Variable magnetic fields (e.g. 50-60 Hz mains transformers, overhead power lines). •...
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Page 23: Operation
If the magnet was cold, this can take up to 1.5 days. Start the PC and subsequently the software Bruker the minispec NF (minispec.exe). The right LED on the electronics unit turns green as soon as the software has recognized the system successfully. -
Page 24: Selecting Applications And Connections
Operation The ‘wake-up’ application can also be selected for future program start-up. When the user has finished with the selections, the OK button is used to start the software. To change the configuration, press the Setup button. The configuration is protected by a simple log-in mechanism. -
Page 25: The Signal Window
Operation Figure 6.1: The View of the Signal Window, Result Window and the Spread Sheet. For the pre-configuration of measurements and a simplified data output a Spread sheet/ database Window is integrated in some applications. Furthermore, the interface layout is adjusted to the individual needs of the analysis for most of the recent standard application fields. -
Page 26: Minispec.exe Menu Functions
Operation Normally the tables are executed after RUN is selected, beginning from row one to the end of the entries. But you can also measure single rows of the table by marking these lines and then selecting RUN. This may be useful if not all samples are prepared at the beginning of the analysis or if several measurements should be repeated. -
Page 27: The File Menu
Operation Adjustment of the bars (i.e. the possibility to move them) depends on the configuration of analyzer type you are working in. Repositioning of bars and windows is only allowed for the relaxation time and the NMR Analyzer. Figure 6.2: The Default View The arrangement of the different bars supports an intuitive measurement handling. - Page 28 Operation The Open Command Shortcuts: Standard Tool Bar: Keys: CTRL+O Use this command to open an existing document in a new window. One can select either: • A complete minispec application (filename extension .app) consisting of maximum four windows: an editor window with ASCII-text in ExpSpel programming language; the Signal Window with the measured signals;...
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Page 29: Figure 6.4: The Dialog Box For Data Saving Setup
Operation The Save Setup Command The Result box displays results derived from the NMR data (after evaluation steps defined in the minispec application). Results are automatically saved as ASCII text at intervals (file extension: .mdt ). To set the parameters for the save procedure, select Save Setup from the result box file menu: Figure 6.4: The Dialog Box for Data Saving Setup Select Default Storing to backup every 100 result box lines of measured data to the file... - Page 30 Operation The Print Command Use this command to print a document. It opens a Print Dialog Box, where you may specify the range of pages to be printed, the number of copies, the destination printer, and other printer setup options. Shortcuts: Standard Tool Bar: Keys: CTRL+P The Print Preview Command...
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Page 31: The Edit Menu
Operation 6.2.4.3 The Edit Menu The list of commands in the Edit pull-down menu depends on the active window (see figure below). In the following description all commands are included to ensure a complete documentation. Figure 6.5: The Edit Pull-Down Menus for Different Active Windows In the figure above, the left side shows the menu when the Signal Window is active, and the right side when the Results Window is active. -
Page 32: The Minispec Menu
The minispec serial number must have the form NFxxxx for mq-systems. The minispec serial number, which is the host name of the minispec, is used to identify the BRUKER minispec, controlled by the minispec software that is running on a PC in a TCP/IP network. -
Page 33: Figure 6.7: The Minispec Settings Table
Operation The Instrument Settings Command Minispec instrument settings are instrument dependent values such as the pulse lengths and the magnetic field at NMR resonance. Instrument settings differ from minispec to minispec, and must be tuned the first time a minispec is connected. You can use this command to change the settings of the instrument with manual input. - Page 34 Operation The Update Settings Command An update may be necessary after the system has been installed, relocated or the probe assembly has been changed. An instrument settings update is also recommended if the Daily Check fails and no other cause of the failure can be localized. One should first check the sample, the cable connections and the sample position inside the probe before running Update Settings.
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Page 35: The Measurement Menu
Use this menu to open the license number input box, which prompts the user to input the license number. After a successful license procedure, the menu entry is checked and grayed. Have ready the minispec instrument's system number (see Status Bar) and contact BRUKER for information or license numbers. 6.2.4.5... - Page 36 Operation Measurement Tool Bar: The command Continue is used to leave an intermediate process in order to continue running the minispec application (e.g. during a waiting time, a data acquisition or a fit display). Shortcuts: Standard Tool Bar: Measurement Tool Bar: Keys: ESC The Calibrate Command A typical minispec application detects NMR signals of the sample and then calculates the...
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Page 37: The Parameter Menu
Operation A flag in the Info Toolbar indicates a calibrated minispec application. An uncalibrated minispec application is labeled with the following flag: The Detect raw Signal Command In some cases, for example to adjust the acquisition parameters, it might be helpful to detect the signal without data processing. -
Page 38: Figure 10: The Acquisition Parameter Table
Operation Experiment parameters that can be edited through the Acquisition Parameters menu: Figure 6.10: The Acquisition Parameter Table Scans (1 – 32000) Scans is used to set the number of times the NMR experiment should be repeated for signal averaging before the result is evaluated. The required number of scans depends on the signal strength, the desired signal-to-noise (which will limit the precision of the measurement), and the desired speed of analysis (time between scans is set by the Recycle Delay). - Page 39 Operation Dummy Shots (0 - 32000) Pulses before the measurement can be made prior to the experiments, in order to ensure that the first scan has the same magnetization history as all subsequent scans. Static Gradient (x, y, z, -100…+100) If a Pulse Gradient Unit is attached, this parameter (typically x) is used to control a static gradient generated with a constant current to the gradient coils.
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Page 40: The View Menu
Operation Repetitive measurements require several control parameters. When this command is selected, a table appears where the following parameters can be specified: the waiting time in seconds before a repetitive minispec measurement begins, the delay between two successive repetitions, and the maximum number of repetitive measurements. 6.2.4.7 The View Menu Each user can configure the screen for the special requirements of a measurement. -
Page 41: Figure 6.12: The Statistics Box
Operation – Electronic Control Unit temperature status (grayed if Control Unit temperature detection is set OFF. – External Gradient Unit status. – Possibility of attenuated and shaped pulses. The entries are marked with a checkmark ( ) when the corresponding item status is OK. Shortcut: Info Tool Bar: 3. -
Page 42: Figure 6.13: Display Modes
Operation 4. Fit Info: You can display a window containing all information of a current fit. An active Fit Window is marked with a check mark. The Display Modes Command Different display modes are available for the measurement signal. Possible modes are: Figure 6.13: Display Modes You can activate the desired display mode with a mouse click. - Page 43 Note: Zooming, data, distance and marker cursors can only be used exclusively. The Welcome Box Command On the BRUKER minispec program start, the software wakes up with the Welcome Box (see also section Configuration of the Software, the Welcome Box). The Welcome Box offers the possibility of adapting some software settings to the user’s purposes (e.g.
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Page 44: The Process Menu
Operation Some of the functions described might only be available in certain minispec.exe software versions. 6.2.4.8 The Process Menu The Process item is only displayed when a Signal Window is active. Different commands for data processing are summarized in this pull-down menu: Figure 6.14: The Process Pull-Down Menu Within the context not all commands may be reasonable, in which case they will be grayed. -
Page 45: The Window Menu
Operation 6.2.4.9 The Window Menu Figure 6.15: The Window Pull-Down Menu Default, Cascade, Tile Horizontal and Tile Vertical Commands You can arrange the windows (see figure) as Cascade in an overlapping fashion, or you may arrange multiple open windows Horizontally or Vertically side by side. To reset the window alignment, use the Default command. -
Page 46: The Help Menu
Operation 6.2.4.10 The Help Menu For a user friendly handling the minispec software includes a detailed help function. You have the choice of different entries to receive help on your questions (see figure). Green entries in the help text are cross references to related topics. One may switch to them by clicking on the green text. -
Page 47: Running Minispec Plus Software
Operation Running minispec Plus Software Double-click on the minispec Plus icon to start the software. Figure 6.17: minispec Plus NF Icon The software will start and come to the log-in screen. Figure 6.18: The minispec Plus Log-in Dialog To get full access to all the menus of minispec Plus, type ADMIN and press the Enter button. You will now be transferred to the main menu of minispec Plus. -
Page 48: Daily Check
Operation 6.3.1 Daily Check As a first action, run the Daily Check function to validate the system. The software will then guide you through the procedure, requesting the Daily Check sample. Insert the corresponding samples with care into your NMR system. Finally, the test should conclude, and the instrument should be valid for 24 hours. -
Page 49: General Measurement Handling
Operation General Measurement Handling The most important commands that are used to control measurements are: the Run command to start a measurement and the Stop command to interrupt a measurement in progress. Measurements can be started through a number of commands and by activating a number of software icons or features. - Page 50 Operation 50 / 72 E164809_2_002...
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Page 51: Basic Nmr Principles
Basic NMR Principles Basic NMR Principles NMR (Nuclear Magnetic Resonance) is made possible by the properties possessed by some atoms that cause their nuclei (part of every atom) to have a magnetic moment. The atom most commonly investigated by NMR is Hydrogen ( H), the nucleus of which is composed of a single proton. -
Page 52: Figure 7.2: Decay Of The Nmr Signal Due To Transverse Relaxation
(SFC) measurements. As a consequence such materials as foodstuff, cosmetics, chemical products and much more can be investigated. For a more detailed description refer to the application descriptions available from Bruker. Furthermore, the intensity of the NMR signal is a function of the number of atomic moments contributing to the macroscopic magnetization. -
Page 53: Nmr Parameters
NMR Parameters NMR Parameters When defining the calibration/method in minispec Plus software, the software prompts typical values for the NMR sequence parameters. However, it might be necessary to adjust these parameters, especially if the method of test differs from the default approach. Then it is useful to adjust the NMR parameters accordingly. -
Page 54: Acquisition Pre-Delay Time
NMR Parameters Acquisition Pre-Delay Time In some cases a defined delay is desired between the time a sample is inserted and the time of measurement start (e.g. if time is needed to allow the temperature of the sample to equilibrate in the magnet). The Acquisition Pre-Delay Time option allows such a delay to be set (in seconds). -
Page 55: Maintenance
Maintenance Maintenance Cleaning Before Cleaning Disconnect the main cable from the unit before cleaning. Cleaning the Main Unit Use a clean damp cloth to wipe off any residue from the outside of the unit. Wipe off excess moisture immediately with a dry cloth. For non water soluble marks, wipe using a cloth with a few drops of isopropyl alcohol. - Page 56 Maintenance 56 / 72 E164809_2_002...
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Page 57: 10 Replacement Of Parts
10.1 Returning the Unit for Repair If the Bruker Hotline diagnoses an instrument failure that requires a part to be returned for repair, please follow the procedure listed here: 1. Contact your local Bruker office to start the repair process (see Contact). Repair is always handled by your local Bruker office. - Page 58 Replacement of Parts 58 / 72 E164809_2_002...
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Page 59: 11 Dismantling And Disposal
Installation, initial commissioning, retrofitting, repairs, adjustments or dismantling of the device must only be carried out by Bruker Service or personnel authorized by Bruker. Damage due to servicing that is not authorized by Bruker is not covered by your warranty. 11.1... -
Page 60: Disposal In Usa And Other (Non-Eu) Countries
European directive WEEE 2012/19/EU. Bruker BioSpin offers to take back the equipment (only for deliveries after 23.03.2006) after termination of use at the customer site upon request by the customer. Additional costs for dismantling and transport service will apply! Only 100% pre-decontaminated equipment can and will be accepted by Bruker BioSpin. -
Page 61: 12 Technical Data
Technical Data 12 Technical Data 12.1 General Information Series Type Weight Length Width Heigth (kg) (cm) (cm) (cm) LF Series LF50 LF Series LF50H* LF Series LF65** LF Series LF90** LF Series LF110** Table 12.1: Technical Data: General Information All specifications above are without the PC. Weights and dimensions apply to the instruments, not to the packaged systems. - Page 62 Technical Data 15-Pin SUB-D Connection 3 2-65 MHz maximum 20 µs 250W- 2.2W CW (CW = continuous power) Input RF receive signal (0 dBm 1 mW) 28V/10.7A DC (Lamda Power Supply) CAN High +5V/DC maximum 150 mA (control board) -5V/DC maximum 150 mA (control board) GND (Lamda power supply) CAN Low GND (control board)
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Page 63: Operating Conditions
Technical Data 12.3 Operating Conditions Environment Series Type Temperatur Temper Relative Decreasing e Range, ature Humidity at Linear Till Safety Maximum/ Range, 31 °C, Relative Class °C Ideal/°C Maximum/% Humidity < 50% at 40°C, maximum/% LF Series LF50 18-28 22-25 80 (non- <... -
Page 64: Figure 12.1: An Example Of A Minispec Rating Plate
Technical Data Figure 12.1: An Example of a minispec Rating Plate 64 / 72 E164809_2_002... -
Page 65: 13 Contact
WEEE DE43181702 Bruker BioSpin Hotlines Contact our Bruker BioSpin service centers. Bruker BioSpin provides dedicated hotlines and service centers, so that our specialists can respond as quickly as possible to all your service requests, applications questions, software or technical needs. - Page 66 Contact 66 / 72 E164809_2_002...
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Page 67: List Of Figures
List of Figures List of Figures Figure 2.1: Example of a Stray Field Plot for the LF90 - Front View..........Figure 2.2: Example of a Stray Field Plot for the LF90 - Top View..........Figure 4.1: The minispec LF50 Analyzer ..................Figure 4.2: The minispec LF50H Analyzer.................. - Page 68 List of Figures 68 / 72 E164809_2_002...
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Page 69: Index
Menu functions .......... 24 Analyzer type............ 23 Application programs........ 24 NMR analyzer........... 24 NMR signal display........... 24 Balance Settings command...... 34 Basic NMR Principles ........ 51 Bruker Service ............ 6 Open Command .......... 28 Operating Environment ........ 63 Calibrate command ........ 36, 49 Cleaning the Main Unit ........ 55 Close Command.......... 28 Parameters .......... 24, 37 Connect/Disconnect Command...... 32... - Page 70 Index Technical Data: .......... 61 Toolbars ............ 26 Transport and handling ........ 15 Unselect All command........ 31 Update Settings .......... 23 Update Settings command ....... 34 User interface ........... 24 Wake-up configuration ........ 23 Welcome Box ........... 23 70 / 72 E164809_2_002...
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