Table Of Contents - GE AxiChrom 50 Operating Instructions Manual

Table of Contents

1 Introduction ..........................................................................................................
1.1 General introduction ...........................................................................................................................
1.2 Scope of this Operating Instructions ...........................................................................................
1.3 Important user information .............................................................................................................
1.4 Regulatory information ......................................................................................................................
2 Safety instructions ...............................................................................................
2.1 Safety precautions ...............................................................................................................................
2.2 Column pressure and over-pressure ...........................................................................................
2.3 Labels .........................................................................................................................................................
3 Specifications and characteristics ....................................................................
3.1 Principles of column function .........................................................................................................
3.2 Primary specifications ........................................................................................................................
3.3 Column lengths and bed support materials ............................................................................
3.4 Materials ...................................................................................................................................................
3.5 Chemical resistance ............................................................................................................................
3.6 Packing flow rates ................................................................................................................................
4 Getting started .....................................................................................................
4.1 Checking the packing list ..................................................................................................................
4.2 Identifying the main components of the column ..................................................................
4.3 Bed supports ...........................................................................................................................................
4.4 Wetting instructions for plastic bed supports .........................................................................
4.5 Other equipment ...................................................................................................................................
4.6 Routine procedures .............................................................................................................................
4.7 Over-pressure situation .....................................................................................................................
4.8 Preparing the column for a run ......................................................................................................
4.9 Installation in basic configuration ................................................................................................
4.10 Installing the UNICORN Intelligent Packing strategy on ÄKTAexplorer and ÄKTApi-
lot .................................................................................................................................................................
4.11 Selecting the Intelligent Packing strategy and component ..............................................
5 Operation ..............................................................................................................
5.1 Intelligent Packing of the column with ÄKTAexplorer and ÄKTApilot ...........................
5.2 Intelligent packing of the column with ÄKTA avant 150 .....................................................
5.2.1 Create a column type .......................................................................................................................
5.2.2 Creating an Intelligent Packing method using ÄKTA avant 150 ....................................
5.2.3 Preparing the slurry, priming and packing the column using ÄKTAavant ................
5.3 Manual packing of the column ......................................................................................................
5.4 Performance evaluation of the column .....................................................................................
5.5 Unpacking a packed column ..........................................................................................................
5.6 Cleaning-in-place (CIP) ......................................................................................................................
5.7 Sanitization ..............................................................................................................................................
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
Table of Contents
5
5
5
6
8
10
10
14
15
17
17
22
23
23
26
29
30
30
31
32
34
35
36
50
53
57
60
61
62
63
79
80
84
93
94
100
102
103
103
3