Agilent Technologies 1200 Series Reference Manual page 40

A Safety Information
Solvent Information
Observe the following recommendations on the use of solvents.
Flow Cell
Long term operation at pH > 11 should be avoided. Never leave strongly
alkaline solutions in the flow cell without flow.
Solvents
Always filter solvents through 0.4 µm filters, small particles can permanently
block filters, frits and capillaries. Avoid the use of the following steel-corrosive
solvents:
• Solutions of alkali halides and their respective acids (for example, lithium
• High concentrations of inorganic acids like sulfuric acid, especially at
• Halogenated solvents or mixtures which form radicals and/or acids, for
• Chromatographic grade ethers, which can contain peroxides (for example,
• Solutions of organic acids (acetic acid, formic acid, and so on) in organic
• Mixtures of carbon tetrachloride with 2-propanol or THF. dissolve stainless
40
iodide, potassium chloride, and so on).
higher temperatures (replace, if your chromatography method allows, by
phosphoric acid or phosphate buffer which are less corrosive against
stainless steel).
example:
→
2CHCl + O 2COCl
3 2
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
THF, dioxane, di-isopropylether) such ethers should be filtered through dry
aluminium oxide which adsorbs the peroxides.
solvents. For example, a 1-% solution of acetic acid in methanol may attack
steel.
steel.
+ 2HCl
2
1200 Series MI Reference Manual