Quantitating Samples - Promega Quantus Fluorometer Operating Manual

3.C. Calibrating the Quantus™ Fluorometer for Use With Other Dye Chemistries
(continued)
2. Place the blank sample into the tube holder, and close the lid. Select "Read
Blank", and the fluorescence in relative fluorescence units (RFU) for the
blank sample will be displayed on the screen.
3. Place the standard sample into the tube holder, and close the lid. Select
"Read Std", and the fluorescence RFU for the standard sample will be
displayed on the screen.
Note: Once both the blank and standard samples are measured, the
standard-to-blank ratio is automatically calculated. If the standard-to-blank
ratio is less than 3, the screen will display "Invalid", and the instrument
will not quantitate unknown samples until the blank and standard samples
are prepared properly (see Section 3.A). User-defined protocols use a
minimum standard-to-blank ratio of 3 as the quality check.
4. Save the calibration data by selecting "Save". Proceed to Section 3.D.
3.D. Quantitating Samples
Note: If quantitating samples of higher concentration than the standard, dilute
the sample to ensure it is within the linear range of the standard.
1. Press any button to wake up the Quantus™ Fluorometer. The Home screen
will appear, displaying the last protocol used, the last concentration
measurement and a list of menu items for navigation.
2. Navigate to the right side of the screen, and select "Protocol" from the
menu list on the Home Screen.
3. Select the desired protocol.
4. Navigate to and select the Volume/Units button to enter the volume of
sample (i.e., template volume) added to the assay.
5. To enter the sample volume, press the up or down buttons to the desired
volume. Choose from 1–10, 15, 20, 25, 50, 100, 150 or 200µl.
Note: This volume is the amount of sample that was added to the assay.
6. To select the concentration units used to display the data, press the up or
down button. Choose ng/μl, ng/ml, µg/ml, mg/ml or Auto.
Note: If Auto is selected, the instrument will automatically assign units that
place the concentration value in the 1.0 to 1,000.0 range.
7. When the desired units are highlighted, press "Enter".
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM396
Page 12
Printed in USA.
Revised 1/20