Error Messages - Promega Quantus Fluorometer Operating Manual

5. Troubleshooting (continued)
Symptoms
Quantus™ Fluorometer powered up,
but the screen was blank or could not
be deciphered or did not respond
when buttons are pressed
Quantus™ Fluorometer was plugged in, Instrument entered sleep mode. Press any
but the screen was dark
5.B. Error Messages
Codes or Messages
Incomplete (on Calibration screen)
Invalid (on Calibration screen)
Valid (on Calibration screen)
Lower than Blank (on the Main screen) The unknown sample was lower than that of the
Saturated (on the Main screen)
Higher than Standard
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Part# TM396
Page 16
Causes and Comments
Unplug USB Cord from the instrument, and
plug the cord back in, making sure no buttons
are pressed during this time.
button to wake up the instrument.
Causes and Comments
The protocol was not calibrated. Calibrate the
protocol from the Calibration screen. See
Section 3.A or 3.C.
The expected standard-to-blank ratio was not
met. Check the preparation of standard and
blank samples to ensure that they were
prepared correctly. Prepare new blank and
standard samples if necessary. Measure
fluorescence again. Press "Save" after
measuring the fluorescence of the new standard
and blank samples to retain the calibration
values.
The standard-to-blank ratio was within the
parameters expected by the instrument. Press
"Save" to retain the calibration values, and
proceed to measure fluorescence of the
unknown samples.
blank sample. LOW will appear on the History
screen, but the raw RFU values will still be
displayed. This indicates no sample was present
in the tube holder or there was a problem with
sample preparation.
Fluorescence was beyond the linear range of the
instrument. SAT will appear on the History
screen for both the concentration and raw RFU
value. Dilute the sample or standard, and
measure fluorescence again.
The unknown sample was over 10% higher than
that of the standard sample. The raw RFU values
will still be displayed on the history screen.
Printed in USA.
Revised 1/20