Promega Quantus Fluorometer Operating Manual page 10

2. Prepare the QuantiFluor
follows. For example, to make a 1:400 dilution, combine 10µl of
QuantiFluor
QuantiFluor
ONE dsDNA (Cat.# E4871), single-
concentration standard curve
dsDNA (Cat.# E2670), single-
concentration standard curve
RNA (Cat.# E3310), high-
concentration standard curve
RNA (Cat.# E3310), low-
concentration standard curve
ssDNA (Cat.# E3190), high-
concentration standard curve
ssDNA (Cat.# E3190), low-
concentration standard curve
3. Prepare the nucleic acid standard in a 0.5ml PCR tube. Use the volume of
supplied standard and volume of 1X TE buffer indicated in the table below
to prepare the standard.
QuantiFluor ® Dye System
ONE dsDNA (Cat.# E4871)
dsDNA (Cat.# E2670)
RNA (Cat.# E3310), high-
concentration standard curve
RNA (Cat.# E3310), low-
concentration standard curve
ssDNA (Cat.# E3190), high-
concentration standard curve
ssDNA (Cat.# E3190), low-
concentration standard curve
Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA
Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com
Printed in USA.
Revised 1/20
® Dye working solution with 1X TE buffer as
® Dye with 3,990µl of 1X TE buffer, and mix.
® Dye System
Starting
Standard
Concentration
400ng/µl
100ng/µl
100ng/µl
100ng/µl
100ng/µl
100ng/µl
Dilution of QuantiFluor
no dilution necessary
(dye is pre-diluted)
1:400
1:400
1:2,000
1:400
1:2,000
Volume of
Volume of Working
Standard Solution
1µl 200µl
2µl 200µl
5µl 200µl
First dilute
1:100 in
1X TE 200µl
Buffer, then
use 10µl
4µl 200µl
First dilute
1:100 in
1X TE 200µl
Buffer, then
use 10µl
® Dye
Final
Standard
Calibration
(per tube)
400ng
200ng
500ng
10ng
400ng
10ng
Part# TM396
Page 9