Hygiena UltraSnap US2020 Quick Start Manual

UltraSnap Surface ATP Test
For use with Hygiena ATP Monitoring Systems
Part No: US2020 (100 tests)
Description/ Intended Use:
UltraSnap Surface ATP Test is a self-contained device for use with
Hygiena luminometers. The test device and luminometer create a
system used for monitoring hygienic status of surfaces on processing
equipment and other environments in a wide range of industries. The
system works by measuring adenosine triphosphate (ATP), the
universal energy molecule found in all animal, plant, bacterial, yeast,
and mold cells. Product residues from organic matter left on surfaces
contain ATP. Microbial contamination on a surface contains ATP but
typically in smaller amounts. After proper cleaning, all sources of ATP
should be significantly reduced. When a sample is collected and ATP
is brought into contact with the unique liquid stable Luciferase/
Luciferin reagent in the UltraSnap test device, light is emitted in direct
proportion to the amount of ATP present in the sample. The
luminometer measures generated light and reports results in Relative
Light Units (RLU). RLU result provides information on the level of
contamination within seconds. The higher the RLU number, the more
ATP present, and the dirtier the surface. It is important to note that
UltraSnap is designed to detect invisible/trace amounts of residue.
Overloading the swab with physical matter by swabbing a visibly dirty
surface will inhibit the bioluminescent reaction and produce
inaccurate results.
For water samples such as Clean In Place (CIP) rinse water testing,
use AquaSnap ATP Test Devices. Visit
information.
Directions:
Instructional Video: www.youtube.com/HygienaTV
Before beginning testing, turn on luminometer. If luminometer has
been programmed with test locations, select appropriate location
before running test.
Allow UltraSnap to equilibrate to room temperature (21 – 25 °C)
1.
before use. Holding swab tube firmly, twist and pull top of swab
out of swab tube. Condensation may be visible on inside of
swab tube; this is normal.
2.
Thoroughly swab a standard 10 x 10 cm (4 x 4 inches) area for
a typical flat surface. Swab tip is pre-moistened for maximum
sample collection. For irregular surfaces, ensure swabbing
technique remains consistent for each test and swab a large
enough area to collect a representative sample.
Important swabbing technique tips:

Do not touch swab or inside of sample device with fingers.

Rotate swab while collecting sample to maximize sample
collection on swab tip.

Apply sufficient pressure to create flex in swab shaft.

Swab in a crisscross pattern vertically, horizontally, and in
both diagonal directions.

Refer to instructional video for demonstration:
www.youtube.com/HygienaTV
3. After swabbing, replace swab back in swab tube.
4. To activate device, hold swab tube firmly and use thumb and
forefinger to break Snap-Valve by bending bulb forward and
backward. Squeeze bulb twice, expelling all liquid down swab
shaft.
Bathe swab bud in liquid by shaking for 5 – 10 seconds. Once
5.
activated, sample must be read in luminometer within 30
seconds.
6.
Holding luminometer upright, insert entire UltraSnap device into
Hygiena luminometer.
Close lid and press "OK" to initiate measurement. Refer to
7.
instrument manual for operating instructions. Results will be
displayed in 15 seconds.
INS0039 EN JUN 2014
www.hygiena.com for more
Rev. B
Interpretation of Results:
Hygiena luminometers are preset with Pass & Fail RLU limits of 10
and 30 RLU. These limits are based on industry standards and
published study recommendations. When using default settings,
readings less than 10 RLU indicate surface is considered clean.
Readings between 11-29 RLU indicate a warning, surface is not
adequately clean. If reading is greater than 30 RLU, surface is
considered dirty. Hygiena recommends setting RLU thresholds
according to standards of your facility. Visit
view recommended practices or call our technical representatives
for guidance.
Calibration Control:
It is advisable to run positive and negative controls according to
Good Laboratory Practices. Hygiena offers the following controls:

(Part # PCD4000) Calibration Control Kit for Hygiena
Luminometers

(Part # CK25) ATP Positive Control Kit for ATP Test Devices
Storage & Shelf Life:

Store at 2 – 8 °C (36 – 46 °F)

Test devices will tolerate temperature abuse for 4 weeks at
room temperature (< 25 °C)

Store UltraSnap devices out of direct sunlight.

Devices have a 15-month shelf life. Refer to expiration date
on label.
Disposal:
UltraSnap devices are made of 100% recyclable plastic and may
be discarded accordingly.
Safety & Precautions:
Components of UltraSnap do not pose any health risk when used
in accordance with standard laboratory practice and procedures of
this insert.

UltraSnap test devices are for one-time use. Do not reuse.
For further safety instruction, refer to Safety Data Sheet (SDS).
Hygiena Liability:
Hygiena will not be liable to user or others for any loss or damage
whether direct or indirect, incidental or consequential from use of
this device. If this product is proven to be defective, Hygiena's sole
obligation will be to replace product or at its discretion, refund the
purchase price. Promptly notify Hygiena within 5 days of discovery
of any suspected defect and return product to Hygiena. Please
contact Customer Service for a Returned Goods authorization
number.
Contact Information:
If more information is required, please visit us at
or contact us at:
Hygiena - Americas Hygiena - International
Phone: 805.388.8007 Phone: +44 (0)1923 818821
Email: info@hygiena.com
Email:
www.hygiena.com to
www.hygiena.com
enquiries@hygiena.com