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Hygiena Dualo 32 Product Instructions
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Hygiena Dualo 32 Product Instructions

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Product Instructions
Dualo 32
®
Product No. MCH230008

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Summary of Contents for Hygiena Dualo 32

  • Page 1 Product Instructions Dualo 32 ® Product No. MCH230008...
  • Page 2 Dualo 32 ® Product No.: MCH230008 Product Instructions: Revision A, September 2023...

  • Page 3: Table Of Contents

    ® TABLE OF CONTENTS TABLE OF CONTENTS This manual will teach you what you need to know to start running your Dualo 32 instrument. ® It describes everything from connecting your instrument to the network to data analysis for your qPCR experiments.

  • Page 4: Setup

    2. Power Supply Unit and Mains Cable 3. Ethernet Cable 4. USB Drive Please keep the flight case and outer box the Dualo 32 came in, in case you need to protect your Dualo 32 during storage or shipment in future. USB Port The lid ensures that tubes are seated correctly in the wells, provides optical isolation, includes a heated lid to reduce condensation, and prevents dust falling into empty wells.
  • Page 5 Cyan Experiment complete Red-Flashing Fault detected Dualo 32 Connections The Dualo 32 has three connections. One in the front and two in back as shown below: Ethernet Port Power Power Ethernet Port This is to connect your Dualo 32 to the This is for connecting your Dualo 32 to provided power supply unit.
  • Page 6 After a few seconds, your instrument will turn on. The display LEDs will light up blue and then turn green if a lid is closed or red if not. Your Dualo 32 is ready to run. The Dualo 32 uses a 3-pin IEC mains connector.
  • Page 7 Dualo 32 Heated Lid The Dualo 32 heated lid will get hot. Please do not touch it. The heated lid will be preheated to 105 ºC if user activity is detected. This enables your run to start as soon as possible. After 5 minutes of inactivity, the heated lid will be turned off to conserve energy.
  • Page 8 MCH230008 - Dualo 32 ® SETUP Configuring your Dualo 32 Please select Configuration Please select Dualo 32 To add your new Dualo 32, select the Add button. You will now be presented with a list of available instruments.
  • Page 9 MCH230008 - Dualo 32 ® SETUP Double-click on the instrument you wish to connect to, or press Select with the instrument selected. The instrument will now be added to the list of Registered Instruments for use in the software.

  • Page 10: Tips

    MCH230008 - Dualo 32 ® TIPS 2. TIPS Here are some great tips to keep in mind while running the Dualo 32 instrument. 1. Lid gets warm 2. Do not leave the lid open 3. Spin the tubes 4. Remove all bubbles 5.
  • Page 11 MCH230008 - Dualo 32 ® TIPS Remove all bubbles Bubbles can cause optical artefacts as shown in the graph below. Ensure that no bubbles are present in reaction volumes. Keep the lab clean Please keep your work space clean, including all lab equipment like surfaces, pipettes and tube racks.

  • Page 12: Experiment

    EXPERIMENT 3. EXPERIMENT This section will teach you everything you need to know to get started with Dualo 32 experiments. You will learn how to create, save, open and close experiments. You will also learn how to set up sample information. Finally we will show you how to run your new experiment from the software, and from the USB drive provided.
  • Page 13 MCH230008 - Dualo 32 ® EXPERIMENT Choose a Template from the list by highlighting it, then click Select. By using a template you will be selecting pre-set parameters - temperature profile, optical and analysis settings to run an experiment. Choose Add to put a new Template to the list.
  • Page 14 MCH230008 - Dualo 32 ® EXPERIMENT Experiment Summary Once you have chosen a new experiment from a template, the Experiment Summary will be displayed as shown below: By default, the experiment name will be “New Experiment” with a date and time stamp, which can be edited.
  • Page 15 MCH230008 - Dualo 32 ® EXPERIMENT Open an Experiment By selecting Open, you can open an experiment as shown below: USB Run To open a USB run, navigate to the USB location using the Open dialog window shown below. Select Experiment File in the Files of Type drop down menu and then double click on the USB run.
  • Page 16 MCH230008 - Dualo 32 ® EXPERIMENT Saving an Experiment Once a run has finished, it can either be automatically saved to a pre-set location on your computer or manually saved by you. The experiment must be saved before the software is closed to ensure that data is not lost.
  • Page 17 MCH230008 - Dualo 32 ® EXPERIMENT Samples You will now learn how to set up samples which can be defined as follows: Samples or targets do not need to be defined before starting a run. They can be added to an experiment, while it is running or once it has finished.
  • Page 18 Starting a Run from the Software To start a run from the Dualo 32 software, select Start Run. You will be presented with the auto-save options (unless you have chosen not to be prompted) and then be asked to choose an instrument from the list of Registered Instruments.
  • Page 19 ® EXPERIMENT Loading Strips into your Dualo 32 In order to ensure that the heated lid is balanced, please make sure that the mount contains at least a strip in rows A and D (as shown in blue below) or single tubes in wells A1, A8, D1 and...

  • Page 20: Analysis

    MCH230008 - Dualo 32 ® ANALYSIS 4. ANALYSIS Depending on the chosen template, different types of analysis are pre-set. Auto Quantification Click on the Auto. Quant. analysis in the Analysis pane on the left hand side of the screen. Quantifiers If you have assigned standards with different known quantifiers to your experiment, for example a dilution series, you will be able to see your reaction efficiency by selecting Quantifiers.
  • Page 21 MCH230008 - Dualo 32 ® ANALYSIS Auto Tm Calling (Melting) Select the analysis Automatic Tm Calling to identify and characterize melting peaks. You can see the results below. Click on the Tm (A) analysis in the Analysis pane on the left hand side of the screen.

  • Page 22: Export

    ® ANALYSIS 5. EXPORT When you have finished analyzing your data in the Dualo 32 software, you can export the results in a variety of ways, from raw data to user defined custom reports. Data Export You can export data from panes with results in tabular format by clicking the Export button.

  • Page 23: Maintenance

    MCH230008 - Dualo 32 ® MAINTENANCE 6. MAINTENANCE This section will help you take care of your Dualo 32. It covers the following areas: 1. Cleaning 2. Environmental Conditions 3. Disassembly Cleaning Day-to-Day For day-to-day cleaning, wipe the external surface of your instrument with a damp, soft, lint-free cloth.
  • Page 24 Your Work Space Your Dualo 32 should be placed on a surface that is flat, dry, and not subject to drafts. Do not install your Dualo 32 instrument directly in the flow of air from an air conditioner or fan. Do not install your Dualo 32 instrument in a dusty environment.

  • Page 25: Troubleshooting

    MCH230008 - Dualo 32 ® TROUBLESHOOTING 7. TROUBLESHOOTING This section will help you troubleshoot your Dualo 32 if you think something is wrong. FAQs Here are some frequently asked questions and answers. Can I leave my Dualo 32 Yes. It is safe to leave your Dualo 32 overnight. It will enter a powered on overnight? low power standby mode.
  • Page 26 TROUBLESHOOTING Error Messages Your Dualo 32 will let you know when something is wrong by displaying an error. Most errors are reported as messages in the Status Bar in the Dualo 32 software. Some errors are reported by the instrument display LEDs.
  • Page 27 MCH230008 - Dualo 32 ® TROUBLESHOOTING Error Messages - Displayed in Status Bar Message Action Run Prepare Error Please follows the instructions in the error (followed by one of the following messages) message and continue as normal. a) No tubes loaded.
  • Page 28 For software issues, go to Configuration > About and select Save Software Logs to File. Hardware Errors For Hardware faults, go to Configuration > Dualo 32 and select Retrieve Instrument Report Files. Once exported, please make sure you send the error file, the serial number and the experiment file that contained the error to Technical Support.

  • Page 29: Warranty And Disclaimer Of Liability

    (2) Hygiena Diagnostics GmbH does not warrant its product against any and all defects when: the defect is as a result of material or workmanship not provided by Hygiena Diagnostics GmbH; defects caused by misuse or use contrary to the instructions supplied, or if the product is contaminated by improper storage or handling.
  • Page 30 MCH230008 - Dualo 32 ® TROUBLESHOOTING Ordering Information Contact your local Hygiena Diagnostics GmbH representative for ordering information. Order Number Product Name MCH230008 Dualo 32 real-time PCR instrument ® Trademarks Dualo 32 is a registered trademark of Hygiena Diagnostics GmbH.
  • Page 31 Hygiena ® Camarillo, CA 93012 diagnostics.support@hygiena.com Manufactured by Hygiena Diagnostics GmbH Hermannswerder 17 14473 Potsdam Germany www.hygiena.com...

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Mch230008