Hygiena Autosampler III User Manual
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Hygiena Autosampler III User Manual

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Autosampler III User Manual
www.hygiena.com
INS4088-REVA

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Summary of Contents for Hygiena Autosampler III

  • Page 1 Autosampler III User Manual www.hygiena.com INS4088-REVA...
  • Page 2 The following aspects must be considered: before carrying out the work, this manual must first be carefully examined. This prevents the Autosampler III from being used incorrectly. It is also advisable to keep this manual with the Autosampler III so that the operator always has access to it.
  • Page 3 Automatic starting machine biological material Warning Warning Warm surface Beware of rotating parts Warning Warning Optical radiation Beware of injuries to the hand Note Reserved for certified engineers Special explanation Interesting tips Operations to be carried out only by certified personnel www.hygiena.com...

  • Page 4: Table Of Contents

    3.1.1. Internal transport ....................... 11 3.1.2. External transport ...................... 11 3.1.3. Lifting points ......................11 3.2. Packaging ........................... 13 3.2.1. Items included with Autosampler III ................13 3.2.2. Preparing for transport ....................14 3.2.3. Packaging exterior Autosampler III ................14 3.3. Storage ..........................14 4.
  • Page 5 5.16. Description of the operator's workplace ................22 5.17. Personal protective equipment ..................22 5.18. Restrictions on the use of the Autosampler III ..............22 6. Standard operating procedure - Autosampler III ................. 23 6.1. Prepare the compressor for use: ..................23 6.1.1.
  • Page 6 11.2.3. Switch cabinets ....................... 65 11.3. Maintenance Instructions pneumatic components ............66 11.3.1. Cylinder........................66 11.3.2. Air service unit ......................66 11.3.3. Couplings and valves ....................66 11.3.4. Air tubing ......................... 66 12. Maintenance service panel visualizations ................. 67 12.1. Introduction ........................67 www.hygiena.com...
  • Page 7 12.10.3. Plate input ......................77 12.10.4. Plate output ......................78 12.10.5. Pipetting arm ......................78 13. Camera jobs ..........................78 13.1. Create new camera job ....................78 13.2. Modify existing camera job ....................86 13.3. Parameter overview ......................88 www.hygiena.com...
  • Page 8 15.1. Definitions ......................... 94 15.2. Lockout rules ........................95 15.3. Sequence to lockout or tagout ..................95 15.4. Sequence to restore the Autosampler III to normal production operations ..... 96 16. Patch management ........................97 16.1. Xperimate ......................... 97 16.2. HMI ........................... 97 16.3.

  • Page 9: Introduction

    This prevents the user from entering the Autosampler III while the system is running and forces the user to make a controlled stop of the Autosampler III. Besides the main lockable access door on the front, the user can also access the pipetting cabinet from the side and from the back.

  • Page 10: Technical Specifications

    Autosampler III, the system's various liquid reservoirs must be filled. Both power connections can be found on the back of the Autosampler III and are connected by means of a power plug and a pressure fitting. For correct functioning of the Autosampler III the drain of the system needs to be attached to the sewage at floor level.

  • Page 11: Pneumatic

    The Autosampler III is equipped with anodized aluminum and stainless steel. Chlorinated agents can affect these areas with prolonged use. When the Autosampler III is cleaned using wet, dry and or chemical means, the system must always be switched off by means of the main switch. Cleaning of the Autosampler III is addressed in Chapter 9 Cleaning the Autosampler III.

  • Page 12: Internal Transport

    3.1.1. Internal transport A pallet lift can be used to move the Autosampler III within a building. Please note the sizes specified in this user manual. If the Autosampler III is to be moved through doors or narrow and/or low passages, this should be done at appropriate speed to avoid damage.

  • Page 13: Packaging

    The Autosampler III can also be lifted from the left and right side, which requires two pallet trucks. Position the forks of the pallet truck below the lifting points on both the left and right side of the Autosampler III. The forks of the pallet truck on the left side need to be placed at a depth of 550-650 mm.

  • Page 14: Preparing For Transport

    Remove the samples and plates from the Autosampler III. Remove all parts that can be dismantled without using tools. 3.2.3. Packaging Exterior Autosampler III To prevent damage to the outside of the Autosampler III, it should be packed with the following materials. Material Image...

  • Page 15: Assembly And Installation

    The assembly and installation of the Autosampler III is carried out by personnel of Hygiena Partners or by an external body that has been authorized to do so by Hygiena. The final check is carried out by Hygiena Partners. The assembly and installation is carried out by qualified and experienced technicians. This chapter is written for the technicians who assemble, install and test the Autosampler III.

  • Page 16: Commissioning Instructions General

    • Make sure the Autosampler III is matched to the correct products. • In order to ensure the safe operation of the Autosampler III, the protective devices, such as the door locks and emergency stop, must be checked. • The fixed guards such as windows and panels must be properly mounted or installed before commissioning.
  • Page 17 Samples enter the machine from an external rolling conveyor. The system separates the carton boxes from each other and aligns the box to the side rail (green) with a push bar (blue). This allows the Autosampler III to work with various box and sample sizes. As the cardboard box is slightly tilted at an angle, filling of the partially filled boxes with material to keep them in place may be needed.

  • Page 18: Machine Control

    LIMS ID. 5.5. Smart vision camera The Autosampler III is equipped with a Cognex smart camera. The camera is used to detect the piercing positions and the grid layout of a box. A barcode camera is placed outside the pipetting cabin to read the barcodes on the boxes containing the products.

  • Page 19: Pipetting

    After the samples and the reagents have been added to the microtiter plate, the samples are homogenized in a shaking routine of five minutes. The shaker conveyor belt allows for each sample to have nearly the exact same chemical reaction time. www.hygiena.com...

  • Page 20: Plate Output

    The Autosampler III has an output buffer to enable continuous processing of products. The output buffer is filled one by one with the push mechanism described above. The buffer is accessible by opening the transparent panels that close off the output buffer.

  • Page 21: Stacklight

    5.12. Stacklight The Autosampler III is equipped with a stacklight stop behind the control panel. The stacklight is used to inform the user on the current status of the machine. This can be used to monitor the system's status when the user is not near the HMI panel (where this information is also displayed).

  • Page 22: Conditions Of Use

    5.18. Restrictions on the use of the Autosampler III The Autosampler III can be used for pipetting sample material from specified packaging to a shallow well plate. Liquids to be pipetted must be water-based and must not contain flammable substances.

  • Page 23: Standard Operating Procedure - Autosampler Iii

    6. Standard operating procedure Autosampler III This chapter describes the daily operations that are to be carried out in order to properly run the Autosampler III. It must be noted that working with any kind of equipment may be dangerous. The Autosampler III is equipped with safety functions that allow for safe working with the system.
  • Page 24 Main switch off Main switch on • Make sure there is sufficient air pressure on the Autosampler III (at least 5 bar is required). This can be read from the manometer which indicates the pressure. Insufficient pressure Sufficient pressure >5 bar •...

  • Page 25: Shutting Down Of The Compressor

    The compressor is now turned off. 6.2. Starting up the Autosampler III In order to start up the Autosampler III and get the Autosampler III ready for a run, please carefully follow the steps listed below. Step 1: Power on the Autosampler III.
  • Page 26 Step 3: Wait for the Autosampler III to start up. After pressing the "Reset" button, the Autosampler III will need up to 45 seconds to start. The status light will be blue when the Autosampler III's start-up is finished. During start-up, the status light will be blinking red, this is due to the soft start of the pressure regulator.

  • Page 27: Initializing The Autosampler Iii

    Before the Autosampler III can run in production mode, the Autosampler III needs to be initialized. The initialization routine is a procedure carried out by the Autosampler III to make sure all electronic parts are in a proper position and are ready to receive commands given by the Xperimate control software. As the initialization routine requires movements to be carried out, the Autosampler III needs to be free from products, dust and samples (spills).
  • Page 28 Perform inspection: • Check if the Autosampler III contains no more products on the conveyor belt. Empty conveyor belt • Check to confirm no plates are in the outfeed roller conveyor is clear from (jammed up) boxes of product. Free roller outfeed •...
  • Page 29 • Check the plate outfeed. Outfeed • Check to confirm surfaces of the Autosampler III are clean according to Chapter 9. • Check that the wash bath is clean according to Chapter 9. • Check that the needles are free from damage.
  • Page 30 Close the pipetting cabinet. Door 1 front side Door 2 rear side Door 3 right side • Close the shaker panel. Shaker panel • Make sure the cover panels are attached. Cover panel 2 Autosampler III infeed. Cover panel 1 outfeed www.hygiena.com...
  • Page 31 • On the interface, press "Lock Doors". If the Autosampler III is locking, a clicking sound is heard from the locking pins. Autosampler III will be energized, air will flow in and moving parts will be turned on. "Lock" button •...

  • Page 32: Starting Autosampler Iii Initialization

    Press the "Start" button. All parts of the Autosampler III will start to initialize and move to their home positions. This means that the conveyor will move slightly forward, the pipetting channels will move up and to the back of the Autosampler III, the shaker will initialize and shake for a short duration.

  • Page 33: Priming The Pumps

    Before samples can be pipetted and reagents can be dosed, the Autosampler III needs to be primed. The priming routine in the Autosampler III ensures that no air is present inside the tubing connecting the liquid containers to the pump and the pump to either the dispense manifold or the pipetting needle.

  • Page 34: Start Priming Method

    The priming method will initialize the pumps and pumps liquid through the tubing to prime the channels. During priming of the pumps, the status light will be blinking blue. When the pump priming is finished, the status light will be blue, indicating the Autosampler III is ready to be used. Autosampler III priming method 6.6.
  • Page 35 Load the plates in the input destacker, the plates can be placed on top of the destacker lift. Make sure the plate barcodes are facing to the right (when standing in front of the Autosampler III). Make sure that plates are properly stacked so that no plate is at an angle.

  • Page 36: Pre-Run

    ATP file is loaded from the LIMS system and made available for the Autosampler III. The ATP file is used to let the Autosampler III know which boxes should be run and information from the ATP is used to create the export file for the Innovate.

  • Page 37: Starting A Run

    After the doors are locked, the operator needs to wait for the air pressure to reach proper level before being able to initialize the Autosampler III and start runs. This is indicated by a blue status light. 6.9. Starting a run In order to start a run, the following procedure should be followed: •...

  • Page 38: Main Run

    Fill the stacker with plates using the "03-LoadPlates" method (see Chapter 6.6 Loading plates). Make sure the plate barcodes are facing towards the right. Place the boxes on the Autosampler III. Make sure the barcode on the box is facing towards the barcode scanner.

  • Page 39: Dummies

    The samples that will be skipped will start from the end of the box. Dummies should be placed from right to left, from bottom to top. Box with dummies should be placed in the Autosampler III as shown above. Dummies can be created using stickers with the following symbol:...

  • Page 40: Manual Po

    The Manual PO method is not standardly activated for use, to activate this method please contact the supplier. After performing all the initializing steps, the Autosampler III is ready to start pipetting samples. For manual sampling of boxes without barcodes, follow the steps listed below.
  • Page 41 Press "Start" to start the method on the Autosampler III. Fill in the PO number and the number of boxes to run. Make sure the boxes numbers are in the ATP file, otherwise the boxes cannot be processed. After the manual PO is finished, the last processed box will automatically move out and the plate will continue automatically.

  • Page 42: Exchange Of Liquids Procedure

    The Autosampler III is equipped with various pumps to perform the liquid handling steps within the Autosampler III. This section explains which pumps are used and how to identify them. This is important to connect the correct chemical container to a pump.

  • Page 43: Chemical Containers

    6.13.2.1. Buffer The chemical (buffer) is placed into the Autosampler III by means of a 50 ml tube. The 50 ml tube has a blue cap that has a hole through which the tubing can be fed.
  • Page 44 6.13.2.2. ATX The chemical, ATX, is placed into the Autosampler III by means of a 50 ml tube. The 50 ml tube has a blue cap that has a hole through which the tubing can be fed. The 50 ml tube is placed in the cooling block. It is important to ensure that the tubing is fed through all the way to the bottom.
  • Page 45 Autosampler III when replacing the chemicals. (See Chapter 6.5) 6.13.2.3. Microwash for pipetting The chemical, microwash, is placed into the Autosampler III by means of a 4-liter container. The 4-liter container has a red cap containing 4 holes through which the tubing can be fed. The 4-liter microwash container is placed in the fluid bay on the left side.
  • Page 46 A plastic bag, tube or vial can be used to prevent liquid from flowing out of the tubing while exchanging the container. Remove the cap from the container and replace the used container for a new one. Contact Hygiena for information regarding the reuse of remaining chemicals.
  • Page 47 Remove the cap from the container and replace the used container for a new one. Information regarding the reuse of chemicals can be requested to Hygiena. When the container is replaced, the cap containing the hole can be screwed back onto the new container.

  • Page 48: End Of Day Procedure

    6.14. End of day procedure This section explains how to take the Autosampler III out of production at the end of a production day. This procedure needs to be performed by the operator after 8 hours of usage or at the end of the day. This process may take up to 30 minutes to perform.

  • Page 49: Daily Validation Procedure

    • The Autosampler III is initialized according to the SOP (Chapter 6.3). • The Autosampler III is primed according to the SOP (Chapter 6.5). • Set up the correct shaker time and speed in the HMI settings (Chapter 10.10). •...

  • Page 50: Replacing Needle(S)

    Step 1: Make sure the Autosampler III is initialized and ready Before replacing the needles, the Autosampler III should be initialized. This ensures the needles can move, to a position where they can easily be replaced. The Autosampler III should also be in idle state, indicated by the blue light.
  • Page 51 Step 7: Check tubing inside the arm Before fastening the needle, make sure the tubing inside the arm is straight and not bent. If the tubing is bent, it can result in positioning errors of the channels. Correct tubing inside arm Incorrect tubing inside arm www.hygiena.com...

  • Page 52: Cleaning The Autosampler Iii

    9. Cleaning the Autosampler III This chapter describes how and when to clean the Autosampler III. For correct functioning of the Autosampler III, it is important to always keep the system clean. Before cleaning the Autosampler III, turn the system off (Chapter 6.14).

  • Page 53: Deck/Stainless Steel

    Window cleaners such as Glassex may also be used. When using ethanol, make sure that the Autosampler III is always turned off. To prevent leaving scratch marks, use a lint free towel and preferably a microfiber cloth. When the stainless steel turns dull overtime, stainless steel cleaning agents may be used to polish the surfaces and make them shiny again.

  • Page 54: Shaker

    9.4. Shaker The shaker is a delicate part of the Autosampler III due to the accuracy required to shake properly. Cleaning of the shaker can be done as described in the stainless steel section. However, take care to not apply too much liquid, i.e., use a damp cloth and not a soaked cloth.

  • Page 55: Pumps (Syringes)

    Clean the inside of the locknut using a lint free towel. Demineralized water can be used. Optionally, ethanol can be used, but only when the Autosampler III is turned off. The goal of the cleaning is to remove any particles such as formed crystals or dust.

  • Page 56: Conveyor Belt

    10. HMI / Xperimate operations panel This chapter will explain the visual parts of the HMI Autosampler III. The HMI is part of the Xperimate platform developed by Synchron. The HMI is an enclosed user interface that will not have access to all the functionalities of Xperimate.

  • Page 57: Sidebar

    • Pre-Run: Always enabled when the Autosampler III is idle and disabled during a run. • Start: Always enabled when the Autosampler III is idle or when a run is paused. The button is disabled during a run. • Pause: Disabled when the Autosampler III is idle. The button will be enabled during a run.

  • Page 58: Recipe Manager

    If the "Cancel" button is pressed, all changes will be discarded. When the "Ok" button is pressed all changes will be applied to the Autosampler III. Each row in the table corresponds to a separate recipe file. When a row is selected, the "Remove"...

  • Page 59: Top Bar

    10.5. General overview In the general tab, a simplified overview of the Autosampler III is visible. In this overview, the data of two boxes will be displayed: •...
  • Page 60 Each reagent has a warning and error parameter in the settings that configures when a corresponding warning or error will be displayed (reagent low or critically low). When the reagents are empty, the "Reset" button should be pressed to let the Autosampler III know that the reagents have been refilled. www.hygiena.com...

  • Page 61: Plate Detail Overview

    In this overview a simplified well plate is displayed. When the window is opened it will always display the current microtiter plate (MTP) in the Autosampler III. The plate is displayed in the orientation in which it will be processed.

  • Page 62: Alarm Manager Overview

    In the alarm overview, a table with all alarms is displayed. Displayed here are which alarms occurred and from where they originated. In order to start a method on the Autosampler III, all alarms need to be acknowledged. The acknowledging of an alarm should only occur when the alarm has been investigated and resolved to ensure that no damage will be done to any personnel or to the Autosampler III.

  • Page 63: User Manager

    In the image to the right, every part of the Autosampler III is colored green. In the case of any errors or alarms, the specific part that encounters problems will be colored differently to reflect the current status. Status colors: •...

  • Page 64: Settings

    Wear the appropriate personal protective equipment such as protective clothing, goggles and gloves. • Handles and the floor around the Autosampler III should be free of dust and liquids to prevent slipping. • Broken or damaged parts must be replaced as soon as possible, consult Hygiena.

  • Page 65: Maintenance Instructions Electrical Components

    When the maintenance work has been completed, the person responsible for this, such as the lab manager, must be satisfied that the work has been completed, that the Autosampler III is back in service, that the protective devices have been installed and that there are no persons within the range of the Autosampler III protections.

  • Page 66: Maintenance Instructions Pneumatic Components

    Reserved for certified engineers. Operations to be carried out only by certified personnel. The Autosampler III has several pneumatic parts that require periodic maintenance and need to be checked regularly for optimal use. This part of the manual explains this in detail.

  • Page 67: Maintenance Service Panel Visualizations

    Every visualization is password protected and will contain a "Main" tab allow login or logout. Username: Admin Password: prepare 12.2.1. Login • Press "Login" to log onto the Autosampler III. • Fill in "Username". • Fill in "Password". • Press "Ok" to login...

  • Page 68: Logout

    12.2.2. Logout • Press "Logout" to logout of the Autosampler III. Now all controls will be disabled again, and it will no longer be possible to control the Autosampler III using the different buttons. 12.3. Actuators Using this visualization, all the different actuators can be controlled.

  • Page 69: Sensors

    1 - Green • 2 - Red • 4 - Blue • 5 - Orange Using the buttons for "Blinking", stack light blinking can be turned on or off. 12.5. Cylinders Using this tab, the different cylinders can be controlled. www.hygiena.com...

  • Page 70: Cylinder Control

    Clamps plates onto the shaker, so they are stuck while shaking. 12.5.2. Stacker pins The stacker pins control is a little different in that there are two pins that can be controlled separately using the "In" and "Out" buttons. www.hygiena.com...

  • Page 71: Pumps

    Toggle control word from 0-129 - Set Anybus active 12.7.3. Status word Show that the status word of the selected NodeID, where Byte 1 is the status of the selected NodeID and Byte 2 is the error byte, indicating which Node has an error. www.hygiena.com...

  • Page 72: Network

    Toggle to let the channel move to an absolute position or a relative position from Abs/rel its current position. (0 = absolute movement, 1 = relative movement) Shaker clamp Clamps plates onto the shaker, so they are stuck while shaking. www.hygiena.com...

  • Page 73: All

    Toggle to let the channel move to an absolute position or a relative position from Deceleration its current position (0-10000 rpm/s). Reset Position Using this button will reset the movement block of the pipetting channels in case of an error. www.hygiena.com...

  • Page 74: Motors

    Sets the specific velocity with which the motor should move. Deceleration Sets the specific acceleration with which the motor should reach target velocity. Jerk Sets the specific deceleration with which the motor should break. Actual Sets the specified jerk (rate of change in acceleration over time). www.hygiena.com...

  • Page 75: Lift Stacker

    3000 12.9.3. Pusher input Using this tab, the pusher input, which moves a plate so the Autosampler III can pipet in the right wells, can be controlled directly. See Chapter 12.9.1. Pusher Input is controlled in the same way.

  • Page 76: Snare Belt

    20000 12.10. Routines Using this visualization, all routines on the PLC can be run. There are 6 tabs: • Main (see Chapter 12.2 Main) • Box Handling • Shaker • Plate Input • Plate Output • Pipetting Arm www.hygiena.com...

  • Page 77: Box Handling

    Reset Init - Resets the homing of the parts used for handling boxes. Get Box - Gets a box from the input and places it under the camera. Move Box Out - Outputs a box from the Autosampler III. Status • Yellow - Warning during routine •...

  • Page 78: Plate Output

    Step 1: Make sure the machine is on and initialized Make sure the Autosampler III and the compressor are switched on and initialized. The Autosampler III should not be running and the Xperimate application needs to be closed, before connecting to the Cognex camera.
  • Page 79 The job can now be saved with the desired name. The location of the job should be on the camera's SD card, otherwise the Autosampler III will not be able to load the job. After saving the template as a new job, check if the new job is opened and start working from there.
  • Page 80 Step 10: Teach pattern to detect Double click on the teaching pattern for the sample (cell "J1") to open the pattern teaching menu. Hide the spreadsheet to have a better view of the captured image, the pattern teaching menu will stay open. www.hygiena.com...
  • Page 81 Incorrect pattern (too much detail) Step 10.3: Reuse training image If the pattern is correct, make sure to select the "Reuse training image" checkbox. Otherwise, the detection pattern will not be saved. Confirm the pattern settings by pressing "Ok". www.hygiena.com...
  • Page 82 Double click on the teaching pattern for the dummy (cell "M1") to open the pattern teaching menu for the dummy detection. Repeat Steps 10.1 until 10.4 to teach the dummy pattern. Use the cells that are listed for the dummy detection. www.hygiena.com...
  • Page 83 Do not lower the Z-axis below 150 mm, otherwise the needles will not be able to position. It is recommended to use low speed and acceleration/deceleration settings during Z-axis movement (see figure below). Moving down the Z-axis should be done with small steps (1-10 mm). www.hygiena.com...
  • Page 84 For the Y positions (needles) a repeating pattern with 4 Y-coordinates can be seen, since the 4 positions om each row will be similar to the 4 positions on the next row. Only the belt distance (X position) will change to move the box to the next row. www.hygiena.com...
  • Page 85 Cognex job. The coordinate in cell "A77" (20|29) corresponds with the 1st position in the box (X1, Y1). The last coordinate of the box (position 24) is located in cell "A100" (312|203) in the example below. www.hygiena.com...

  • Page 86: Modify Existing Camera Job

    Step 1: Make sure the machine is on and initialized Make sure the Autosampler III and the compressor are switched on and initialized. The Autosampler III should not be running and the Xperimate application needs to be closed, before connecting to the Cognex camera.
  • Page 87 To open a job, click on "File" and "Open Job" and select the job that needs change. The selected job will now be opened. Step 6: Change the parameters Change the parameters that need to be adjusted. For a list of parameters and their description see Chapter 2. www.hygiena.com...

  • Page 88: Parameter Overview

    >0 (dummy pattern) see Chapter 13 Step 10.4 Acceptance threshold for dummy Accept threshold detection. Must be higher than 75 to prevent 75-100 (dummy patterns) false detections. Using a too high value might cause some samples not being detected. www.hygiena.com...

  • Page 89: Errors And Warnings

    A warning is indicated by a blinking yellow light but does not stop the system. The Autosampler III continues to run if the warning is not blocking the Autosampler III to continue. All warnings are added to the alarm manager. If user input is required, a pop-up will appear on the HMI screen, indicating the actions that need to be taken.
  • Page 90 Process is stopped. • Remove box. • Acknowledge alarm in the alarm manager. • Initialize the system before Autosampler III can be restarted. • Popup in HMI with message "Box output blocked." • Remove box. Box stuck at output Warning •...
  • Page 91 • Acknowledge alarm in the alarm manager. Error movement error • Initialize the system before Autosampler III can be restarted. • If problem persists, contact support. • Warning is added to the alarm manager "Pipetting + Belt loop mismatch, check boxes for missed samples."...
  • Page 92 Error with shaker • If problem persists, contact support. • Autosampler III can still be run using the method for running without shaker. • Error is added to Alarm Manager "Shaker could not initialize". • Alarm needs to be acknowledged before Autosampler III can be restarted.
  • Page 93 Error • Acknowledge alarm in the alarm manager. reagent pumps • Initialize the system before Autosampler III can be restarted. • If problem exists, contact support. • Warning is added to Alarm Manager "Warning: Reagent volume 1, Reagent 1 empty Warning less than 'volume' mL available".

  • Page 94: Lockout Tagout

    Lockout is the preferred method of isolating the Autosampler III from energy sources. It shall be used to ensure that the Autosampler III is isolated from all potentially hazardous energy and locked out or tagged out before associates perform any servicing or maintenance activities where the unexpected energization, start-up or release of stored energy could cause bodily injury and/or damage the Autosampler III or the environment.

  • Page 95: Lockout Rules

    This is the placement of a tagout device on an energy-isolating device, according to an established procedure, clearly marked by means of a tag that states who has the Autosampler III shut down and that the Autosampler III must not be operated until the tagout device is removed by the associate who place it there.

  • Page 96: Sequence To Restore The Autosampler Iii To Normal Production Operations

    Autosampler III to ensure that no one is exposed. After all tools have been removed from the Autosampler III, guards have been reinstalled and associates are in the clear, remove all lockout or tagout devices. Notify all affected persons that the lockout or tagout has been removed.

  • Page 97: Patch Management

    Autosampler III should be verified to ensure that all components are working correctly. This should be done during a run of the Autosampler III. This run should be at least one full MTP. If specific behavior is changed with the patch, that behavior should also be specifically tested.

  • Page 98: List Of Drivers

    16.5. List of drivers To ensure the correct working of the Autosampler III, all the required drivers for the machine should also be installed and configured. This list can be found in the next section. Driver name Application/Configuration COM9: Pipetting pumps Advantech EKI Device Configuration Utility v3.02...

  • Page 99: Decommissioning And Disposal

    19. Decommissioning and disposal As a rule, Hygiena will not provide any patches for the Autosampler III. If the customer deems a patch Special operations are often necessary to temporarily put installations out of operation. Safety plays an important role in this.

  • Page 100: Appendix I: Camera Flow Including Barcode Processing

    Add fixed pierce Operator to confirm positions from camera the amount of job to samples dummies is correct “Box cancelled. Moving box out. Pierce box “Box ok according to user. Process box.” Outfeed box and do not pierce Actor www.hygiena.com...
  • Page 101 Start Split barcode in PO number and Box number Combination PO number and Box PO number found number found in in ATP file ATP file Load recipe Outfeed box and do not pierce. Give warning to operator and log. www.hygiena.com...

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