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Summary of Contents for Hygiena foodproof RoboPrep 32
- Page 1 RoboPrep® 32 Operation Manual Product No. MCH230000 Revision A, March 2024 www.hygiena.com INS-MCH230000-REVA...
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Page 2: Table Of Contents
Operation Procedure ......................... 17 5.2.2 Pause and Restart Procedure ......................21 5.2.3 Stop Procedure ..........................21 Protocol Editing ............................... 22 Editing Menu ............................22 Editing Options ............................23 Cleaning and Maintenance ..........................24 Cleaning Procedures ..........................24 foodproof RoboPrep 32 INS-MCH230000-REVA... - Page 3 Maintenance Schedule ........................... 24 Troubleshooting .............................. 25 Supplementary Information ..........................25 Liability ..............................25 Trademarks ............................26 Copyrights .............................. 26 Contact and Support ..........................26 Reference Number ..........................26 10. Change Index ..............................26 foodproof RoboPrep 32 INS-MCH230000-REVA...
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Page 4: Introduction
RoboPrep 32 is equipped with an exclusive temperature control module that perfectly connects with the 96 Deep-Well Plate to enhance lysis and elution efficiency. For further information or assistance, contact your distributor or the Hygiena® Diagnostics team. 1.3 Applications RoboPrep 32 purifies DNA and RNA from a variety of samples that are broadly used in molecular biology, genetic screening, sequencing, food safety and other applications. -
Page 5: Safety Information
Shipping/Transportation Instructions for detailed information). Never attempt to modify the RoboPrep 32 system without the manufacturer's permission. Do not place or drop objects on the RoboPrep 32 system. Also, refrain from bumping or knocking into it. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 6: Poisonous And Harmful Substances
The RoboPrep 32 system should only be repaired by authorized representatives or agents who use original spare parts certified by Hygiena Diagnostics. If the equipment is used in a manner not specified by the manufacturer, the protection provided by ... -
Page 7: Intended Use And Specifications
–20 to 55 °C (–4 to 131 °F) Relative Humidity <80 °C (<176 °F) Dimension and Weight Dimension 400 x 420 x 440 mm (15.7 x 16.5 x 17.3 in) Weight 25 kg (55.1 lb) EN61326-1 Safety EN60101-1, EN60101-2 foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 8: Instrument Overview
3. Instrument Overview Front View Touch Panel Handler Door Window Rear View Fixing Block Sensor USB Port Rear Cover Power Switch Power Inlet foodproof RoboPrep 32 INS-MCH230000-REVA... - Page 9 Inside View UV Lamp Magnetic Pillar Mixing Sleeves Track Mixing Sleeves Reagent Plate Temperature Control foodproof RoboPrep 32 INS-MCH230000-REVA...
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Page 10: Installation
Ensure all components are free of damage upon receipt. If any damage is found, contact your local representative for support. Note: Hygiena Diagnostics’ warranty does not cover damage from either transportation or improper handling and operation. Labware Use MLD230045, a consumable pack of 12 x DWP (deep well plates) and 24 x 8 Tip Combs, PP (polypropylene) Note: Labware must be purchased separately. -
Page 11: Initial Setup
Do not hold the door or the plastic outer covering while transporting the instrument. Caution: Improper handling of the RoboPrep 32 will lead to instrument damage. foodproof RoboPrep 32 INS-MCH230000-REVA... - Page 12 Use a Phillips-head screwdriver to release the screws. b. Remove the Fixing Block. c. Place the Fixing Block in the upper part of the back of the instrument in the Fixing Block Sensor Spot. This is required for the instrument to operate. foodproof RoboPrep 32 INS-MCH230000-REVA...
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Page 13: Transportation And Shipping Of Roboprep 32
2. Disconnect the plug from the outlet and remove all attached parts (e.g., power cord) from the instrument. Warning: A damaged power cable may cause device failure, fire, injury or electric shock. 3. Pack the RoboPrep 32 instrument, including accessories, in its original shipping box or another equivalent packaging. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 14: Functional Descriptions
Functional Descriptions 4.6.1 Main Menu Home UV Light Light Protocol Settings Hibernate Edit foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 15: Protocol Editing
4.6.2 Protocol Editing After pressing , the icon will change colors , and you can select ‘1’ to ‘12’ to edit your protocol. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 16: Led Light
UV Light After pressing , press ′30 minute′ to start the default 30-minute decontamination process or press ′User Setting′ to set your custom time. 4.6.5 Settings After pressing , you can adjust, check or test the instrument. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 17: Sleep Mode
Operation 5.2.1 Operation Procedure 1. Ensure that the power cable is securely connected to the RoboPrep 32 and the electrical outlet. foodproof RoboPrep 32 INS-MCH230000-REVA... - Page 18 2. Turn the power switch on and wait for the Touch Panel to display ′RoboPrep 32 Initializing′. The system will automatically run self-testing procedures and go into ready mode. Note: The system must complete self-testing before the main functions can be run. 3. Open the door. foodproof RoboPrep 32 INS-MCH230000-REVA...
- Page 19 4. Set up the mixing sleeves on the mixing sleeve track. Caution: Make sure the sleeves have entered completely. 5. Add samples and reagents to the 96 Deep-Well Plate as required for your experiment. 6. Place the reagent plate(s) into the instrument. foodproof RoboPrep 32 INS-MCH230000-REVA...
- Page 20 9. When the extraction procedure is finished, transfer the extracted products located in wells 6 and 12 into nuclease-free tubes. Note: Store the purified nucleic acid at 4 °C (short-term, less than 10 days) or aliquot and store at –70 °C (long-term) before performing any downstream analysis. foodproof RoboPrep 32 INS-MCH230000-REVA...
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Page 21: Pause And Restart Procedure
2. To restart the procedure, press 5.2.3 Stop Procedure 1. If you want to stop the procedure, press 2. Then, press 3. A prompt to Interrupt the program will be displayed. 4. Press ‘Yes’ to end the program. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 22: Protocol Editing
. The icon will change colors , and you can select ‘1’ to ‘12’ to edit your protocol. 2. Select the desired procedure by using the touch screen to make edits or create a new procedure. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 23: Editing Options
Volume The reagent buffer volume of each well. Mix Speed The speed of mixing sleeves during the mixing procedure; choose from 3 different speeds. The time for which the magnetic pillar adsorbs the magnetic beads. foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 24: Cleaning And Maintenance
Two types of maintenance must be performed on the RoboPrep 32 instrument as described in the following table: Maintenance Type Performed by Schedule Routine cleaning of the instrument body Outside User Bi-weekly Inside User Daily Preventive Service engineer Annually foodproof RoboPrep 32 INS-MCH230000-REVA... -
Page 25: Troubleshooting
(1) The product is used according to the guidelines and instructions set forth in the product literature. (2) Hygiena Diagnostics GmbH does not warrant its product against any and all defects when: the defect is as a result of material or workmanship not provided by Hygiena Diagnostics GmbH; defects caused by misuse or use contrary to the instructions supplied, or if the product is contaminated by improper storage or handling. -
Page 26: Trademarks
(6) Hygiena Diagnostics GmbH reserves the right to replace or allow credit for any modules returned under this warranty. Trademarks foodproof®, microproof®, vetproof®, ShortPrep®, StarPrep®, RoboPrep® and LyoKit® are registered trademarks of Hygiena Diagnostics GmbH. Hygiena® is a registered trademark of Hygiena. Other brand or product names are trademarks of their respective holders. - Page 27 Hygiena® Camarillo, CA 93012 diagnostics.support@hygiena.com Manufactured by Hygiena Diagnostics GmbH Hermannswerder 17 14473 Potsdam Germany www.hygiena.com foodproof RoboPrep 32 INS-MCH230000-REVA...
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