Dispensing By Volume: How To Get Started - Parker SciLog LabTec Installation, Operating, & Maintenance Instructions

4.0 Dispensing by Volume: How to get started

Select your pump tubing; use #24 or #15 Silicone tubing if you have a 1082 peristaltic pump head.
Select #24 tubing if you intend to dispense aliquots larger than 10 mL, use #15 pump tubing if you
intend to dispense aliquots smaller than 10 mL.
Consult, Volume Dispense Mode: Edit, go to the following display:
After having connected the tubing to the solution reservoir, fill the tubing with solution and expel all
air from the tubing by pressing the "PRIME" key at the LabTec™ front panel. You are now ready to
setup your dispense using the built-in calibration; go to the following display and press "Edit" key.
Select the following parameters for "Edit 1":
"DISP VOLUME"
"TUBING"
"SNIFFLE"
"SLOW FACTOR"
"PUMP RATE"
"TIME DELAY"
"COUNT"
First press "Exit", then press "Exec" (key "A"), and then "Select" for Exec 1. The following display
will appear:
When you press the "RUN" key, the LabTec™ will dispense one (1) 50.00 mL aliquot.
At this point you may want to check the accuracy of the LabTec™. For this purpose use an electronic
scale with 0.01-gram readability, e.g. Mettler PG5002-S or equivalent. Dispense at least three
50.00 mL aliquots of distilled water, record the weight of each aliquot, and then determine the aver-
age aliquot weight. For example, assume that you obtained the following results:
After you have dispensed the third aliquot, press the RE-CAL key, the LabTec™ will show the following
display:
Use the "Incr." And "Decr." Keys until AV = 50.80, the average aliquot size, then press "Select". The
Calibration has now been corrected and will compensate for the inaccuracy.
enter "50.00 mL"
choose "24"
enter "0.3"
enter "2.5 mL"
enter "80%"
enter "0.1 sec"
enter "1"
VOLUME SET:
Press RUN When Ready
Trial 1 50.80g
Trial 2 50.50g
Trial 3 51.10g
AV = Average Value: 50.80g
DV: 50.00
Incr.
Edit Prime
50:00 mL
AV: 50.00
Decr. Select
73